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    Isolation and purification of the R18 antigen of HTLV-III
    74.
    发明授权
    Isolation and purification of the R18 antigen of HTLV-III 失效
    分离和纯化HTLV-III的R18抗原

    公开(公告)号：US5063053A

    公开(公告)日：1991-11-05

    申请号：US556999

    申请日：1990-07-25

    申请人： Flossie Wong-Stal ,  Pranab K. Chanda ,  John Ghrayeb

    发明人： Flossie Wong-Stal ,  Pranab K. Chanda ,  John Ghrayeb

    IPC分类号： A61K38/00 ,  C07K14/16 ,  C07K16/10

    CPC分类号： C07K14/005 ,  C07K16/10 ,  A61K38/00 ,  C12N2740/16322

    摘要： The isolation and purification of a newly discovered gene of the AIDS virus, HTLV-III, which encodes a portein which is immunogenic and recognized by sera of some HTLV-III seropositive people. Furthermore, the gene is highly conserved among all known HTLV-III isolates and exhibits a polymorphism at the 3' end which distinguishes molecular clones of the HTLV-IIIB cell line from viral genomes of related viruses.

    摘要翻译： 分离和纯化新发现的艾滋病病毒基因HTLV-III，其编码一种免疫原性并被一些HTLV-III血清阳性人的血清识别的portein。 此外，该基因在所有已知的HTLV-III分离物中是高度保守的，并且在3'末端显示出多态性，其区分HTLV-IIIB细胞系的分子克隆与相关病毒的病毒基因组。

    Virus-free in vitro assay for anti-HIV agents
    75.
    发明授权
    Virus-free in vitro assay for anti-HIV agents 失效
    抗病毒药物的无病毒体外试验

    公开(公告)号：US4910132A

    公开(公告)日：1990-03-20

    申请号：US49051

    申请日：1987-05-12

    申请人： David M. Knight ,  John Ghrayeb

    发明人： David M. Knight ,  John Ghrayeb

    IPC分类号： G01N33/569

    CPC分类号： G01N33/56988

    摘要： A virus-free assay to identify agents that interfere with the life cycle of HIV which comprises: (1) expressing in mammalian cells in the presence or absence of one or more of said agents, HIV gp120, the gene product of HIV tat, the gene product of HIV art/trs, all under the control of an HIV LTR promoter, and a control protein, not under the control of the HIV LTR promoter, such that infectious HIV particles are not produced; (2) quantitating the amount of the gp120 produced relative to the amount of control protein produced; and (3) comparing the relative amount of the gp120 produced in the presence of said one or more agents to the relative amount of the gp120 produced in the absence of said one or more agents. A plasmid capable of expressing the gene product of HIV art/trs in mammalian cells such that infectious HIV particles are not produced, which includes: (1) a DNA fragment comprising an HIV LTR promoter, and (2) and HIV cDNA restriction fragment carrying the art/trs gene but not the ATG start codon of the first coding exon for tat.

    摘要翻译： 用于鉴定干扰HIV生命周期的试剂的无病毒测定法，其包括：（1）在存在或不存在一种或多种所述药物HIV gp120，HIV tat的基因产物， 全部由HIV LTR启动子控制的HIV技术/ trs的基因产物和不在HIV LTR启动子控制下的对照蛋白，使得不产生感染性HIV颗粒; （2）定量相对于产生的对照蛋白的量产生的gp120的量; 和（3）将所述一种或多种试剂存在下产生的gp120的相对量与在所述一种或多种试剂不存在下产生的gp120的相对量进行比较。 能够在哺乳动物细胞中表达HIV art / trs的基因产物的质粒，使得不产生感染性HIV颗粒，其包括：（1）包含HIV LTR启动子的DNA片段，和（2）携带 艺术/ trs基因，但不是第一个编码外显子的ATG起始密码子。