公开(公告)号：US20020146733A1

公开(公告)日：2002-10-10

申请号：US10077621

申请日：2002-02-15

申请人： Board of Regents, The University of Texas System

发明人： Kathryn F. Sykes ,  Stephen Albert Johnston

IPC分类号： C12Q001/68 ,  G01N033/53 ,  C12P019/34 ,  C12N015/87 ,  C12P021/02

CPC分类号： C12Q1/6897 ,  C12N15/63 ,  C12N15/64 ,  C12N15/82

摘要： The present invention relates to linear expression elements (LEEs) and circular expression elements (CEEs), which are useful in a variety of molecular biology protocols. Specifically, the invention relates to the use of LEEs and CEEs to screen for gene function, biological effects of gene function, antigens, and promoter function. The invention also provides methods of producing proteins, antibodies, antigens, and vaccines. Also, the invention relates to methods of making LEEs and CEEs, and LEEs and CEEs produced by such methods.

公开(公告)号：US20020132347A1

公开(公告)日：2002-09-19

申请号：US10112090

申请日：2002-03-29

发明人： Jian-yun Dong

IPC分类号： C12Q001/70 ,  C07H021/04 ,  C12P021/06 ,  C12N013/00 ,  C12N015/00 ,  C12N015/09 ,  C12N015/63 ,  C12N015/70 ,  C12N015/74 ,  C12N005/00 ,  C12N005/02 ,  C12N005/06 ,  C12N005/16 ,  C12N015/85 ,  C12N015/87

CPC分类号： C12Q1/6883 ,  C12Q1/703 ,  C12Q2600/158 ,  G01N33/56988

摘要： a method is provided for detecting a presence of HIV virus in a sample comprising: taking a culture of recombinant cells which (a) are capable of cell division, (b) express CD4 receptor and one or more additional cell surface receptors necessary to allow the HIV virus to infect, (c) enable the HIV virus to replicate and infect the noninfected cells in the cell culture, and (d) comprise a reporter sequence introduced into the recombinant cells comprising a reporter gene whose expression is regulated by a protein specific to HIV viruses which is expressed from a genome of an HIV virus upon infection of the recombinant cell by the HIV virus; contacting the cell culture with a sample to be analyzed for the presence of HIV virus in the sample; and detecting a change in a level of expression of the reporter gene in cells in the recombinant cell culture. The method can be used to detect the presence of HIV virus in a sample, detect the presence of different strains of HIV virus in a sample, detect HIV drug resistance in a sample, determine what combination of one or more anti-HIV agents would be effective in treating a patient, and screen compositions for anti-HIV activity.

摘要翻译： 提供了一种用于检测样品中HIV病毒存在的方法，其包括：（a）能够细胞分裂的重组细胞的培养，（b）表达CD4受体和一种或多种另外的细胞表面受体，其允许 HIV病毒感染，（c）使HIV病毒复制并感染细胞培养物中的未感染细胞，（d）包含引入重组细胞的报道序列，其包含报道基因，其表达受特异性蛋白质 HIV病毒在HIV病毒感染重组细胞时由HIV病毒基因组表达的HIV病毒; 使细胞培养物与待分析样品中样品中HIV病毒的存在接触; 并检测重组细胞培养物中细胞中报道基因表达水平的变化。 该方法可用于检测样品中HIV病毒的存在，检测样品中HIV病毒的不同菌株的存在，检测样品中的HIV耐药性，确定一种或多种抗HIV药物的组合将是 有效治疗患者，筛选组合物用于抗HIV活性。

公开(公告)号：US20020132342A1

公开(公告)日：2002-09-19

申请号：US10013587

申请日：2001-12-10

发明人： David Ronald Fitzpatrick ,  Eileen Rose Roux ,  Charles Richard Maliszewski ,  Jacques Jean Peschon

IPC分类号： C12N005/08 ,  C12N015/87

CPC分类号： G01N33/5088 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0331 ,  A01K2267/0381 ,  A61K2035/124 ,  A61K2039/5154 ,  A61K2039/5158 ,  C12N5/0639 ,  C12N15/8509 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/26 ,  C12N2503/02 ,  C12N2510/04 ,  C12N2517/02 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5026 ,  G01N33/5029 ,  G01N33/5047 ,  G01N33/5073 ,  G01N33/5094

摘要： Immortalized mammalian dendritic cell lines are provided that have characteristics of functional, mature dendritic cells; defined by surface phenotype, constitutive and inducible gene expression patterns, and functional effects on T lymphocytes. The cell lines are capable of continuous in vitro proliferation for extended periods of time. Of particular interest is the ability of the cell lines to present antigen in a stimulatory manner to naive T cells. The dendritic cell lines are useful in gene discovery, the generation of dendritic cell subset-specific antibodies and probes; defining stages of dendritic cell differentiation and maturation; for induction of antigen specific stimulation of lymphocytes; for dissection of antigen specific anergy or tolerance in lymphocytes; for characterization of dendritic cell-natural killer cell interactions; for in vitro and in vivo analyses of dendritic cell-endothelial cell interactions; and for screening assays.

摘要翻译： 提供具有功能性，成熟树突状细胞特征的永生化哺乳动物树突状细胞系; 由表面表型，组成型和诱导型基因表达模式定义，以及对T淋巴细胞的功能影响。 细胞系能够长时间连续的体外增殖。 特别令人感兴趣的是细胞系以刺激性方式将抗原呈递给初始T细胞的能力。 树突状细胞系可用于基因发现，树突状细胞亚群特异性抗体和探针的产生; 确定树突状细胞分化和成熟的阶段; 用于诱导淋巴细胞的抗原特异性刺激; 用于解剖淋巴细胞中的抗原特异性无反应性或耐受性; 用于表征树突状细胞 - 自然杀伤细胞相互作用; 用于体外和体内分析树突状细胞 - 内皮细胞相互作用; 并用于筛选测定。

公开(公告)号：US20020132257A1

公开(公告)日：2002-09-19

申请号：US10062707

申请日：2002-01-31

发明人： Tony Giordano ,  Catherine J. Pachuk ,  C. Satishchandran

IPC分类号： C12Q001/68 ,  C12N015/87 ,  C12N005/06

CPC分类号： C12Q1/6876 ,  C12N15/1086 ,  C12N15/1096 ,  C12Q1/6897 ,  C12Q2600/136 ,  C12Q2600/16 ,  C12Q2600/178 ,  G01N33/6866 ,  G01N2333/56 ,  G01N2333/565 ,  G01N2333/57 ,  G01N2500/10

摘要： Described herein are methods for identifying nucleic acid sequences that modulate the function of a cell, the expression of a gene in a cell, or the biological activity of a target polypeptide in a cell. The methods involve the use of double stranded RNA expression libraries, double stranded RNA molecules, and post-transcriptional gene silencing techniques.

摘要翻译： 本文描述了用于鉴定调节细胞功能，细胞中基因表达或细胞中靶多肽的生物活性的核酸序列的方法。 该方法涉及使用双链RNA表达文库，双链RNA分子和转录后基因沉默技术。

公开(公告)号：US20020127723A1

公开(公告)日：2002-09-12

申请号：US10090036

申请日：2002-02-28

发明人： Gianpiero D. Palermo

IPC分类号： C12M001/42 ,  C12N015/87

CPC分类号： C12M35/02 ,  C12N15/873

摘要： The present invention is directed to an electrofusion microelectrode used in the alignment, manipulation, fusion, or electroporation of cells. This device is particularly useful for transplantation of cells and cellular components.

摘要翻译： 本发明涉及用于细胞对准，操作，融合或电穿孔的电融合微电极。 该装置对细胞和细胞组分的移植特别有用。

公开(公告)号：US20020127715A1

公开(公告)日：2002-09-12

申请号：US09995452

申请日：2001-11-27

发明人： Nissim Benvenisty ,  Ofra Yanuka ,  Maya Schuldiner ,  Rachel Eiges-Avner

IPC分类号： C12N005/08 ,  C12N015/87

CPC分类号： C12N15/87 ,  A61K48/00 ,  C12N5/0606 ,  C12N2510/00

摘要： Methods are provided for altering gene expression in a population of human embryonic cells that include introducing a gene expression altering sequence into cells the cells retaining their pluripotent character, for purifying pluripotent embryonic stem cells from a heterogeneous population of cells, and for treating a human suffering from a deficiency of a selected cell type. Reagent cell populations are further provided for supplying material for transplantation consisting essentially of pluripotent human embryonic stem cells modified by foreign genetic material.

摘要翻译： 提供了用于改变人类胚胎细胞群体中的基因表达的方法，包括将保留其多能性的细胞的基因表达改变序列引入细胞，从异源细胞群中纯化多能胚胎干细胞，以及治疗人类的苦难 从选择的细胞类型的缺陷。 进一步提供试剂细胞群用于提供用于移植的材料，其基本上由外源遗传物质修饰的多能人胚胎干细胞组成。

公开(公告)号：US20020119129A1

公开(公告)日：2002-08-29

申请号：US10109885

申请日：2002-04-01

申请人： Yeda Research and Development Co. Ltd.

发明人： Michel Revel ,  Carolina Abramovitch ,  Judith E. Chebath

IPC分类号： A61K048/00 ,  C12N015/87

CPC分类号： C12N9/1007 ,  A61K38/21 ,  C07K14/4702 ,  C07K14/555 ,  A61K2300/00

摘要： Novel proteins IR1B1 and IR1B4 have been isolated which bind to the type I IFN receptor IFNAR1 and function in the cellular response to IFNs. DNA encoding such proteins in either the sense or anti-sense orientation can be administered to either enhance or inhibit the cellular response to IFNs. Antibodies to the proteins can be used for isolation of the new protein or for immunodetection thereof.

摘要翻译： 已经分离出新的蛋白质IR1B1和IR1B4，其结合I型IFN受体IFNAR1并在细胞对IFN的反应中起作用。 可以施用编码有义或反义方向的蛋白质的DNA，以增强或抑制对IFN的细胞应答。 蛋白质的抗体可用于分离新蛋白质或用于免疫检测。

公开(公告)号：US20020115217A1

公开(公告)日：2002-08-22

申请号：US09981121

申请日：2001-10-17

申请人： SmithKline Beecham Corporation

发明人： Frank Fan ,  Yinduo Ji ,  Howard Kallender ,  Tong Li ,  Damien McDevitt

IPC分类号： C12Q001/00 ,  C12Q001/68 ,  C12N015/87

CPC分类号： G01N33/5023 ,  C12N9/1096 ,  G01N33/5008

摘要： This invention relates to newly developed methods for discovering therapeutic compounds using a cell-based assay system. This invention also relates to compositions of matter useful in carrying out the methods of the invention as well as therapeutic compounds developed using such methods.

摘要翻译： 本发明涉及新开发的使用基于细胞的测定系统发现治疗化合物的方法。 本发明还涉及用于实施本发明方法的物质组合物以及使用这些方法开发的治疗化合物。

公开(公告)号：US20020115216A1

公开(公告)日：2002-08-22

申请号：US09861014

申请日：2001-05-19

发明人： Clifford J. Steer ,  Betsy T. Kren ,  Cheryle Linehan-Stieers ,  R. Scott McIvor ,  Perry B. Hackett

IPC分类号： A61K048/00 ,  C07H021/04 ,  C12N015/87

CPC分类号： A61K47/645

摘要： The present invention provides cationic polymers that include a primary amine and a targeting group covalently bound to the primary amine, wherein the targeting group targets a cell of interest by interacting with the surface of the cell. The invention also provides molecular complexes that include a polyethyleneimine and a targeting group covalently bound to a primary amine of the polyethyleneimine, and a biologically active compound. The invention further provides methods for delivering a biologically active compound to a vertebrate cell.

摘要翻译： 本发明提供阳离子聚合物，其包括伯胺和与伯胺共价结合的靶向基团，其中靶向组通过与细胞表面相互作用而靶向感兴趣的细胞。 本发明还提供分子复合物，其包括聚乙烯亚胺和共价结合聚乙烯亚胺的伯胺的靶向基团和生物活性化合物。 本发明还提供了将生物活性化合物递送至脊椎动物细胞的方法。

公开(公告)号：US20020111472A1

公开(公告)日：2002-08-15

申请号：US09030847

申请日：1998-02-26

发明人： JIAN NI ,  REINER GENTZ ,  STEVEN M. RUBEN

IPC分类号： C07H021/04 ,  C12N015/00 ,  C12N015/09 ,  C07K007/00 ,  C12N015/63 ,  C12N015/70 ,  C12N015/74 ,  C07K005/00 ,  C07K016/00 ,  C07K017/00 ,  A61K038/00 ,  C07K001/00 ,  C07K014/00 ,  C12P021/08 ,  C12N015/85 ,  C12N015/87

CPC分类号： C07K14/52 ,  C12N2799/026

摘要： The present invention relates to a novel T1-R ligand III protein which is a member of the IL-1RI ligand-like family. In particular, isolated nucleic acid molecules are provided encoding the human T1-R ligand III protein. T1-R ligand III polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of T1-R ligand III activity. Also provided are diagnostic methods for detecting immune system-related disorders and therapeutic methods for treating immune system-related disorders.

摘要翻译： 本发明涉及作为IL-1RI配体样家族成员的新型T1-R配体III蛋白。 特别地，提供编码人T1-R配体III蛋白的分离的核酸分子。 也提供T1-R配体III多肽，载体，宿主细胞和用于制备它们的重组方法。 本发明还涉及用于鉴定T1-R配体III活性的激动剂和拮抗剂的筛选方法。 还提供了用于检测免疫系统相关疾病的诊断方法和用于治疗免疫系统相关疾病的治疗方法。