公开(公告)号：US20220235101A1

公开(公告)日：2022-07-28

申请号：US17595894

申请日：2020-05-30

申请人： Whitehead Institute for Biomedical Research

发明人： Ben Waldman ,  Sebastian Lourido

IPC分类号： C07K14/45 ,  C12N1/10 ,  A61K39/002 ,  A61P33/02 ,  C12N15/74 ,  A61P37/04

摘要： The present disclosure provides genetically altered protozoan parasites comprising a mutation in a bradyzoite formation deficient 1 (BFD1) gene, wherein the mutation inhibits differentiation of the parasite into a bradyzoite. The genetically altered protozoan parasites can be utilized in vaccine compositions and in methods of treating apicomplexan parasite infection.

公开(公告)号：US11286455B2

公开(公告)日：2022-03-29

申请号：US16824302

申请日：2020-03-19

申请人： Nancy Leland

发明人： Nancy Leland

IPC分类号： C12M1/00 ,  C12N1/02 ,  C12N1/10 ,  C12N1/12 ,  C12N1/20 ,  C12N13/00

摘要： Methods, devices and kits for the physical separation of plankton into its component parts utilizing phototactic behavior are described. The methods utilize positive phototactic behavior and negative contrast orientation of the zooplankton for maximal in situ separation of phytoplankton and zooplankton for use in further studies and evaluation of separation efficiency. The devices provide effective conditions for use in the separation of plankton into component parts.

公开(公告)号：US11253559B2

公开(公告)日：2022-02-22

申请号：US16252338

申请日：2019-01-18

申请人： UNIVERSITY OF PITTSBURGH—OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION

发明人： Stephen Howard Thorne ,  Daniel J. Byrd

IPC分类号： A61K35/768 ,  A61P35/00 ,  A61K9/00 ,  C07K14/07 ,  C07K14/16 ,  C07K14/47 ,  C12N15/62 ,  C12N9/16 ,  C12N9/10 ,  C12N1/10 ,  C07K16/28

摘要： This disclosure relates to modified viruses, e.g., oncolytic vaccinia viruses, which have been modified to contain an exogenous nucleic acid that expresses a protein that modulates STAT3 activity. It is based, at least in part, on the discovery that vaccinia viruses modified to contain nucleic acid encoding PIAS3 and that express PIAS3 or a fragment thereof can inhibit STAT3 activity and enhance the anti-cancer activity of the vaccinia virus. Accordingly, this disclosure provides for oncolytic vaccinia viruses and methods of using them in the treatment of cancers.

公开(公告)号：US20210332322A1

公开(公告)日：2021-10-28

申请号：US17367231

申请日：2021-07-02

申请人： Huvepharma Inc.

发明人： Mary Ann Pfannenstiel ,  Jennifer Albrecht ,  Glen M. Wilkinson

IPC分类号： C12N1/10 ,  A61K39/012 ,  C12N1/04

摘要： Compositions are provided that contain sporulated coccidial oocysts, wherein the compositions are free of antibiotics and comprise formalin at a concentration sufficient to inhibit microbial growth for at least 12 months while maintaining oocyst viability. Methods of preparing such compositions are also provided.

公开(公告)号：US20210164991A1

公开(公告)日：2021-06-03

申请号：US16954187

申请日：2019-01-16

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

发明人： Stephen JOHNSTON ,  Lu WANG

IPC分类号： G01N33/68 ,  C12N7/00 ,  C12N1/10 ,  C12N1/20 ,  C07K14/47

摘要： A method of inducing a generalized immune response, includes administering to a subject an immunologically effective amount of one or more isolated immunogenic peptides comprising an amino acid sequence of SEQ. ID NOs: 1-46, thereby inducing a generalized immune response to infection by a pathogen in a subject. A prophylactic or therapeutic composition for inducing a generalized immune response, includes an immunologically effective amount of one or more isolated immunogenic peptides comprising an amino acid sequence of SEQ. ID NOs: 1-46. A method of distinguishing a subject infected with a pathogen from an uninfected subject, includes detecting an antibody in the subject that selectively binds one or more isolated immunogenic peptides comprising an amino acid sequence of SEQ. ID NOs: 1-46, wherein the presence of the antibody indicates that the subject is infected with the pathogen. A general method to discover broadly protective components for a vaccine is also disclosed.

公开(公告)号：US10920261B2

公开(公告)日：2021-02-16

申请号：US14882742

申请日：2015-10-14

申请人： Mara Renewables Corporation

发明人： Kevin Berryman ,  Zhiyong Sun ,  Michael Milway ,  Mercia Valentine ,  Roberto E. Armenta

IPC分类号： C12Q1/06 ,  C12N1/10 ,  C12N1/04 ,  C12P7/64

摘要： Provided herein are methods of culturing a microorganism. The method includes providing a container comprising one or more microorganisms and medium, wherein the microorganisms and medium form a start volume. The microorganisms and medium are cultured until the culture reaches a threshold indicator, wherein culturing comprises feeding one or more carbon sources to the culture and wherein the culture is at a threshold volume when the threshold indicator is reached. The method also includes harvesting a portion of the threshold volume to leave a residual volume that is 40% or less of the start volume and adding fresh medium to the container in an amount to return the volume of the culture to the start volume.

公开(公告)号：US20200205413A1

公开(公告)日：2020-07-02

申请号：US16537436

申请日：2019-08-09

申请人： NO SELF S.R.L.

发明人： Stefano Mazzoleni

IPC分类号： A01N57/16 ,  C12M1/00 ,  C12N1/10 ,  C12N1/12 ,  C12N1/14 ,  C12N1/20 ,  C12N5/04

摘要： The present disclosure describes a DNA fragment mixture for the prevention or for the treatment of at least one pathogenic, parasitic or infesting species of plants or of the environment, wherein the DNA mixture consists of random fragments of total DNA of at least one pathogenic, parasitic, or infesting species, and/or at least one phylogenetically similar species, against which the prevention and treatment are directed. Further, the disclosure describes a process and related system for improvement of the production/growth of microorganisms at high yield in bioreactors or photobioreactors, or of plants in different culture systems, where the nucleic acids of the organisms produced/grown by such a process are removed from the culture medium and the culture medium, deprived of these nucleic acid, is used again in the process.

公开(公告)号：US10676762B2

公开(公告)日：2020-06-09

申请号：US16264009

申请日：2019-01-31

申请人： Easel Biotechnologies, LLC

发明人： Yi-Xin Huo ,  Kwang Myung Cho

IPC分类号： C12N1/00 ,  C12N1/10 ,  C12P7/64 ,  C12P7/16 ,  C12P39/00 ,  C12M1/40 ,  C12M1/00 ,  C12P13/06 ,  C12P7/56 ,  C12P13/08

摘要： Methods and systems for the production of alcohols are described. A two stage process is utilized, where fermentation in a first stage produces an intermediate product, such as an amino acid or organic acid, from a carbon containing feedstock. A second stage produces alcohol by fermentation of this intermediate product.

公开(公告)号：US10662418B2

公开(公告)日：2020-05-26

申请号：US15918786

申请日：2018-03-12

申请人： MARA Renewables Corporation

发明人： Alexandra Merkx-Jacques ,  David Woodhall ,  Mark Scaife ,  Roberto E. Armenta ,  Denise Muise ,  Holly Rasmussen ,  Jeremy Benjamin

IPC分类号： C12N9/92 ,  C12N9/12 ,  C07K14/40 ,  C12P7/64 ,  C07K14/38 ,  C12N1/12 ,  C12N1/10

摘要： Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.

公开(公告)号：US10557176B2

公开(公告)日：2020-02-11

申请号：US16313906

申请日：2016-08-05

申请人： Beijing Institute of Radiation Medicine

发明人： Ying Yang ,  Shengqi Wang ,  Zhe Zhou ,  Min Chen ,  Peng Wang ,  Zhen Li ,  Zongwei Li

IPC分类号： C12Q1/6895 ,  C12N15/10 ,  C12Q1/6869 ,  C12N1/10

摘要： A method for detecting trace fungi using single-cell sequencing and a fungi detection kit prepared by the method are disclosed. The method includes the steps of obtaining trace fungal cells, extracting fungal protoplasm by breaking the cell walls of fungi, extracting gDNA from trace fungal protoplasm and amplifying the gDNA, constructing trace gDNA library, genome sequencing, bioinformatics analysis and comparison, and determining the species of detected fungi, etc. The method realizes the high-efficiency detection of trace fungi, and can be directly applied to the isolation, detection, identification of trace and difficult-to-identify fungal samples or mixed samples, and in-depth study of genetic information. The fungi detection method and the kit are applicable to industrial production, environmental monitoring, air testing, soil testing, water quality testing, food testing, drug testing, cosmetics testing, health care products testing and medical testing, and other fields.