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    Protein structure-based protein language models
    83.
    发明授权
    Protein structure-based protein language models 有权

    公开(公告)号：US11538555B1

    公开(公告)日：2022-12-27

    申请号：US17533091

    申请日：2021-11-22

    申请人： Illumina, Inc. ,  Illumina Cambridge Limited

    发明人： Tobias Hamp ,  Hong Gao ,  Kai-How Farh

    IPC分类号： G16B30/00 ,  G16B20/50 ,  G16B20/20 ,  G16H50/20

    摘要： The technology disclosed relates to determining pathogenicity of nucleotide variants. In particular, the technology disclosed relates to specifying a particular amino acid at a particular position in a protein as a gap amino acid, and specifying remaining amino acids at remaining positions in the protein as non-gap amino acids. The technology disclosed further relates to generating a gapped spatial representation of the protein that includes spatial configurations of the non-gap amino acids, and excludes a spatial configuration of the gap amino acid, and determining a pathogenicity of a nucleotide variant based at least in part on the gapped spatial representation, and a representation of an alternate amino acid created by the nucleotide variant at the particular position.

    FLOW CELLS AND METHODS FOR MAKING THE SAME
    84.
    发明申请
    FLOW CELLS AND METHODS FOR MAKING THE SAME 有权

    公开(公告)号：US20220379305A1

    公开(公告)日：2022-12-01

    申请号：US17825945

    申请日：2022-05-26

    申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

    发明人： Brinda Kodira Cariappa ,  Wayne N. George ,  Sahngki Hong ,  Lewis J. Kraft

    IPC分类号： B01L3/00 ,  G03F7/039 ,  G03F7/11 ,  G03F7/038

    摘要： In an example of a method for making a flow cell, a metal material is sputtered over a transparent substrate including depressions separated by interstitial regions to form a metal film having a first thickness over the interstitial regions and having a second thickness over the depressions, the second thickness being about 30 nm or less and being at least ⅓ times smaller than the first thickness. A light sensitive material is deposited over the metal film; and the metal film is used to develop the light sensitive material through the transparent substrate to define an altered light sensitive material at a first predetermined region over the transparent substrate. The altered light sensitive material is utilized to generate a functionalized layer at the first predetermined region or at a second predetermined region over the transparent substrate.

    METHODS FOR IMPROVING NUCLEIC ACID CLUSTER CLONALITY
    85.
    发明申请
    METHODS FOR IMPROVING NUCLEIC ACID CLUSTER CLONALITY 有权

    公开(公告)号：US20220333178A1

    公开(公告)日：2022-10-20

    申请号：US17699573

    申请日：2022-03-21

    申请人： Illumina Cambridge Limited ,  Illumina, Inc.

    发明人： Gary Mark Skinner ,  Geraint Evans ,  Niall Gormley ,  Jonathan Boutell ,  Matthew W. Kellinger ,  Michael Previte ,  Molly He

    IPC分类号： C12Q1/6848 ,  C12Q1/70

    摘要： A method for seeding and amplifying target nucleic acids derived from a sample in a cluster at a site on a surface of a substrate includes retaining at least a portion of the target nucleic acids in an inactive form that cannot seed to provide a relatively low concentration of active form target nucleic acids available for seeding. As the active form target nucleic acids seed on the surface of the substrate, they may be amplified. Because the concentration of active form target nucleic acids is low, the likelihood is low that a second active form target nucleic acid will seed at the same site within the same cluster before the first active form target nucleic acid is sufficiently amplified to dominate. Accordingly, the likelihood that the cluster will pass filters is increased relative to traditional seeding and amplification methods employing a higher concentration of active form target nucleic acids.

    METHODS AND COMPOSITIONS FOR NUCLEIC ACID SEQUENCING
    87.
    发明申请
    METHODS AND COMPOSITIONS FOR NUCLEIC ACID SEQUENCING 有权

    公开(公告)号：US20220195518A1

    公开(公告)日：2022-06-23

    申请号：US17555945

    申请日：2021-12-20

    申请人： Illumina Cambridge Limited

    发明人： Xiaolin WU ,  Carole ANASTASI ,  Geraint EVANS ,  Xiaohai LiU

    IPC分类号： C12Q1/6874

    摘要： Embodiments of the present disclosure relate to methods, kits and compositions for two-channel nuclei acid sequencing using blue and violet light excitation (e.g., lasers at 450-460 nm and 400-405 nm respectively). In particular, the nucleotides may be directly labeled with a blue dye, a violet dye, or both a blue dye and a violet dye. Alternatively, one or more nucleotides for incorporation may be unlabeled and affinity reagents containing a blue dye, a violet dye, or both a blue dye and a violet dye may be used to bind specifically to each type of nucleotides incorporated.

    IMAGE SENSOR STRUCTURES AND RELATED METHODS
    88.
    发明申请
    IMAGE SENSOR STRUCTURES AND RELATED METHODS 有权

    公开(公告)号：US20220186307A1

    公开(公告)日：2022-06-16

    申请号：US17643454

    申请日：2021-12-09

    申请人： Illumina, Inc. ,  Illumina Cambridge Limited

    发明人： Lewis KRAFT ,  Craig Lee HETHERINGTON ,  Craig M. CIESLA ,  Michael BUREK ,  Jeffrey FISHER ,  Jason BETLEY

    IPC分类号： C12Q1/6869 ,  H01L27/146

    摘要： An image sensor structure includes an image layer having an array of light detectors disposed therein. A device stack is disposed over the image layer. An array of light guides is disposed in the device stack. Each light guide is associated with a light detector. An array of nanowells is disposed over the device stack. Each nanowell is associated with a first light guide of the array of light guides. A first primer set is disposed throughout a first well region of each nanowell. A second primer set is disposed throughout a second well region of each nanowell. The second well region is adjacent the first well region. The first and second primer sets are operable to attach a forward strand cluster of forward polynucleotide strands in the first well region and a reverse strand cluster of reverse polynucleotide strands in the second well region.

    HYDROGEL
    89.
    发明申请
    HYDROGEL 有权

    公开(公告)号：US20220185927A1

    公开(公告)日：2022-06-16

    申请号：US17600072

    申请日：2020-12-01

    申请人： ILLUMINA CAMBRIDGE LIMITED

    发明人： Gianluca Andrea Artioli ,  Andrew A. Brown ,  Wayne N. George ,  Colin Pilkington ,  Jem Pitcairn ,  Alexandre Richez ,  Xavier von Hatten

    IPC分类号： C08F220/60 ,  C08F2/38

    摘要： A hydrogel includes a dendritic core with 2 to 30 arms, and first and second acrylamide monomers incorporated into each arm. The first acrylamide monomer is: (I), wherein R1 and R2 are independently selected from an alkyl, an alkylamino, an alkylamido, an alkylthio, an aryl, a glycol, and optionally substituted variants thereof; and the second acrylamide monomer is: (II), wherein R3 and R4 are independently hydrogen or an alkyl; L is a linker including a linear chain of 2 to 20 atoms selected from carbon, oxygen, and nitrogen and optional substituents on the carbon and any nitrogen atoms; A is an N substituted amide: (III), where R5 is hydrogen or an alkyl; E is a linear chain of 1 to 4 atom(s) selected from carbon, oxygen and nitrogen, and optional substituents on the carbon and any nitrogen atoms; and Z is an optional nitrogen containing heterocycle.