公开(公告)号：US08494779B2

公开(公告)日：2013-07-23

申请号：US12519791

申请日：2007-12-21

Applicant: Sang Yup Lee ,  Hawoong Jeong ,  Tae Yong Kim ,  Pan-Jun Kim ,  Kwang Ho Lee

Inventor： Sang Yup Lee ,  Hawoong Jeong ,  Tae Yong Kim ,  Pan-Jun Kim ,  Kwang Ho Lee

IPC: G06F19/10 ,  G06F19/12

CPC classification number: C12Q1/18 ,  G01N33/5038

Abstract: The present invention disclosed is a method for screening metabolites essential for the growth of microorganism using metabolic flux analysis. More specifically, the present invention relates to the method for screening metabolites essential for the growth of microorganism, by selecting a target microorganism, constructing a metabolic network model of the selected microorganism, inactivating the consumption reaction of each of metabolites in the constructed metabolic network model, analyzing the metabolic flux of the metabolites to select metabolites essential for the growth of the microorganism, and confirming the selected metabolites using the utilization of each of the metabolites, defined as flux sum (Φ). According to the present invention, metabolites essential for the growth of microorganism, and genes involved in the essential metabolites, can be screened in a convenient manner, and drug-target genes against pathogenic microorganisms can be predicted by deleting genes associated with the metabolites screened according to the method.

Abstract translation: 所公开的本发明是使用代谢通量分析来筛选微生物生长所必需的代谢物的方法。 更具体地说，本发明涉及通过选择靶微生物，构建所选择的微生物的代谢网络模型，在构建的代谢网络模型中灭活各代谢物的消费反应来筛选微生物生长所必需的代谢物的方法 分析代谢物的代谢通量以选择对于微生物生长至关重要的代谢物，并且使用定义为通量总和（Phi）的每种代谢物来确认所选择的代谢物。 根据本发明，可以以便利的方式筛选微生物生长所必需的代谢物和基本代谢物所涉及的基因，并且可以通过删除与代谢物相关的基因来预测针对病原微生物的药物靶基因 的方法。

公开(公告)号：US08486670B2

公开(公告)日：2013-07-16

申请号：US13658369

申请日：2012-10-23

Applicant: Kwang Ho Lee ,  Jae Yeong Ju ,  Ji Sun Lee ,  Young Bin Hwang ,  Sung Hoo Jhon ,  Eun Sung Koh ,  Chul Ha Kim ,  Soo An Shin

Inventor： Kwang Ho Lee ,  Jae Yeong Ju ,  Ji Sun Lee ,  Young Bin Hwang ,  Sung Hoo Jhon ,  Eun Sung Koh ,  Chul Ha Kim ,  Soo An Shin

IPC: C12P13/08

CPC classification number: C12P13/08 ,  C12N9/1085 ,  C12N9/88

Abstract: An L-threonine-producing Escherichia coli in which a promoter of a phosphoenolpyruvate carboxylase (ppc) gene on the chromosome is substituted with a promoter of a cysteine synthase (cysK) gene and a method of producing L-threonine by using the same are disclosed. The recombinant Escherichia coli may produce L-threonine in a high yield, and thus may be widely used in medical, pharmaceutical, and feed industries, particularly for an animal feed.

Abstract translation: 公开了其中染色体上的磷酸烯醇式丙酮酸羧化酶（ppc）基因的启动子被半胱氨酸合成酶（cysK）基因的启动子取代的产生L-苏氨酸的大肠杆菌以及通过使用它们产生L-苏氨酸的方法 。 重组大肠杆菌可以以高产率生产L-苏氨酸，因此可广泛用于医药，制药和饲料工业，特别是用于动物饲料。

公开(公告)号：US08261397B2

公开(公告)日：2012-09-11

申请号：US12298515

申请日：2007-04-20

Applicant: Kwang Ho Lee

Inventor： Kwang Ho Lee

IPC: B08B9/02

CPC classification number: B08B9/045

Abstract: The present invention relates to a flexible wire (100), which is inserted in a pipe and rotated in a high speed to remove scale produced in a pipe by striking. A flexible wire (100) for removing pipe scales comprising: a main wire (110) having plural wires twisted in a spiral direction as one body, wherein the main wire (110) has an overall straight shape; and an auxiliary wire (120) having plural wires twisted in a spiral direction as one body, of which a diameter is substantially smaller than a diameter of the main wire (110), wherein the auxiliary wire (120) is secured to the main wire (110) and has an overall repeated S-shape.

Abstract translation: 本发明涉及一种柔性线（100），其插入管中并高速旋转以通过冲击除去在管中产生的垢。 一种用于除去管鳞的柔性线（100），包括：主线（110），其具有沿着螺旋方向扭曲的多条线作为一体，其中所述主线（110）具有整体直线形状; 以及辅助线（120），其具有沿着螺旋方向扭绞的多根线，作为一体，其直径大致小于所述主线（110）的直径，其中所述辅助线（120）固定到所述主线 （110）并且具有总体重复的S形。

公开(公告)号：US20120122163A1

公开(公告)日：2012-05-17

申请号：US13254072

申请日：2010-02-16

Applicant: Jae Yeong Ju ,  Kwang Ho Lee ,  Hyun Ae Bae

Inventor： Jae Yeong Ju ,  Kwang Ho Lee ,  Hyun Ae Bae

IPC: C12P13/08 ,  C12N1/21

CPC classification number: C12P13/04 ,  C07K14/26 ,  C12N9/1205 ,  C12P13/08

Abstract: The present invention relates to a microorganism belonging to the genus Escherichia sp. and a method for producing L-amino acid using the same. The microorganism belonging to the genus Escherichia sp. has a sucrose assimilability and L-amino acid producing ability, which is obtained by introducing a gene encoding a sucrose assimilative microorganism-derived sucrose metabolic enzyme to sucrose non-assimilative microorganism belonging to the genus Escherichia sp. having an L-amino acid producing ability and sucrose PTS (phosphoenolpyruvate dependent sucrose phosphotransferase system) activity.

Abstract translation: 本发明涉及属于埃希氏菌属的微生物。 以及使用其制造L-氨基酸的方法。 属于埃希氏菌属的微生物 具有蔗糖同化性和L-氨基酸产生能力，其通过将编码蔗糖同化微生物的蔗糖代谢酶的基因导入属于埃希氏菌属的蔗糖非同化微生物而获得。 具有L-氨基酸生产能力和蔗糖PTS（磷酸烯醇丙酮酸依赖性蔗糖磷酸转移酶系统）活性。

公开(公告)号：US20110116952A1

公开(公告)日：2011-05-19

申请号：US12847759

申请日：2010-07-30

Applicant: Jeawoong Yi ,  Seung Yong Lee ,  Gyuhwan Kim ,  Yun Seok Kim ,  Yong Sun Park ,  Tae-Sung Oh ,  Kyung-Hwan Kim ,  Jong-Hoon Lee ,  Kwang-Ho Lee

Inventor： Jeawoong Yi ,  Seung Yong Lee ,  Gyuhwan Kim ,  Yun Seok Kim ,  Yong Sun Park ,  Tae-Sung Oh ,  Kyung-Hwan Kim ,  Jong-Hoon Lee ,  Kwang-Ho Lee

IPC: F01P5/12 ,  H02K5/128

CPC classification number: F04D13/064 ,  H02K5/128

Abstract: An electric water pump apparatus may include a body having a stator chamber and a rotor chamber therein, a stator having a hollow cylindrical shape and being disposed in the stator chamber and generating a magnetic field, wherein the stator fluidly insulates the stator chamber and the rotor chamber, a rotor disposed in the rotor chamber and enclosed by the stator, wherein the rotor is rotated by the magnetic field, and a pump cover connected to the body and forming a volute chamber therein, wherein the volute chamber and the rotor chamber are fluidly-communicated through a connecting hole formed to the body and a coolant is supplied to the rotor chamber through the connection hole, wherein the stator includes a stator groove formed in an inner circumference therein and the stator groove is fluid-connected to the rotor chamber and the volute chamber through the connection hole.

Abstract translation: 电动水泵装置可以包括具有定子室和转子室的主体，具有中空圆柱形状的定子，并且设置在定子室中并产生磁场，其中定子将定子室和转子 设置在转子室中并由定子包围的转子，其中转子通过磁场旋转，以及连接到主体并在其中形成蜗壳室的泵盖，其中涡流室和转子室是流体流动的 通过形成在车身上的连接孔，并通过连接孔将冷却剂供给到转子室，其中定子包括形成在其内周的定子槽，定子槽与转子室流体连接， 蜗壳室通过连接孔。

公开(公告)号：US20100303668A1

公开(公告)日：2010-12-02

申请号：US12445822

申请日：2006-12-28

Applicant: Kwang Ho Lee

Inventor： Kwang Ho Lee

IPC: C22C11/10 ,  C22C30/04

CPC classification number: C22C30/04 ,  C22C11/10 ,  F16K17/383

Abstract: The present invention relates to a fusible alloy for pressure relief devices (PRDs), and the present invention provides a fusible alloy for PRD, the alloy comprising 29.0 to 33.0% by weight of Bi, 14.0 to 21.0% by weight of Sn, 2.0 to 5.0% by weight of In, and substantially Pb for the balance.The fusible alloy for PRD of the invention has a melting point appropriate for use according to the 110° C.-grade standards, and has excellent wettability upon melting.

Abstract translation: 本发明涉及一种用于压力释放装置（PRD）的熔融合金，本发明提供一种用于PRD的可熔合金，该合金包含29.0至33.0重量％的Bi，14.0至21.0重量％的Sn，2.0至 5.0重量％，余量为大致Pb。 本发明的PRD用熔融合金，根据110℃级标准，具有适合使用的熔点，熔融时润湿性优异。

公开(公告)号：US20100089400A1

公开(公告)日：2010-04-15

申请号：US12385337

申请日：2009-04-06

Applicant: Kwang Ho Lee

Inventor： Kwang Ho Lee

IPC: A62B9/02

CPC classification number: F16K1/305 ,  Y10T137/2544 ,  Y10T137/87241

Abstract: Provided is a valve apparatus for a movable oxygen cylinder which has a function of a post valve for an oxygen generation system for household use and that of a post valve for general medical care, selectively. The valve apparatus includes: a valve body whose one side is combined with the movable oxygen cylinder, and which communicates with a discharge port at the other side thereof along a path from one side thereof lengthily therein, to thus discharge out the oxygen of the oxygen cylinder through the discharge port, and which includes an opening which opened outwards from an insertion space provided along the path; a post valve which is installed at the other side of the valve body and controls discharge of oxygen through the discharge port from the oxygen cylinder according to operation of a lever; and a charge adapter whose one side is detachably installed in the opening of the insertion space, and which charges oxygen supplied from the outside into the oxygen cylinder when the post valve closes the path, and discharges oxygen of the oxygen cylinder through the discharge port when the post valve opens the path.

Abstract translation: 提供一种用于可动氧气瓶的阀装置，其具有用于家用氧气产生系统的柱阀和用于一般医疗保健的后阀的功能。 阀装置包括：阀体，其一侧与可动氧气缸组合，并且在其另一侧与其长度方向上的排出口连通，从而排出氧气的氧气 圆柱体通过排出口，其包括从沿着路径设置的插入空间向外开口的开口; 一个安装在阀体另一侧的柱阀，根据杆的操作控制通过排出口的氧气从氧气瓶排出; 以及充电适配器，其一侧可拆卸地安装在插入空间的开口中，并且当柱阀闭合路径时将从外部供应的氧充入氧气瓶中，并且当氧气瓶通过排出口排出氧气时， 柱阀打开路径。

公开(公告)号：US07686036B1

公开(公告)日：2010-03-30

申请号：US12385337

申请日：2009-04-06

Applicant: Kwang Ho Lee

Inventor： Kwang Ho Lee

IPC: F15B13/04

CPC classification number: F16K1/305 ,  Y10T137/2544 ,  Y10T137/87241

Abstract: The valve apparatus includes: a valve body whose one side is combined with the movable oxygen cylinder, which communicates with a discharge port at the other side thereof along a path from one side thereof lengthily therein, to thus discharge out the oxygen of the oxygen cylinder through the discharge port. An opening which opens outwards from an insertion space provided along the path, and a post valve which is installed at the other side of the valve body and controls discharge of oxygen through the discharge port from the oxygen cylinder according to operation of a lever. A charge adapter whose one side is detachably installed in the opening of the insertion space, which charges oxygen supplied from the outside into the oxygen cylinder when the post valve closes the path, and discharges oxygen of the oxygen cylinder through the discharge port when the post valve opens the path.

Abstract translation: 阀装置包括：阀体，其一侧与可动氧气缸组合，其沿着其一侧的长度方向上与其另一侧的排出口连通，从而排出氧气瓶的氧气 通过排放口。 从沿着路径设置的插入空间向外开口的开口，以及安装在阀体另一侧的柱阀，根据杆的操作，通过排气口从氧气瓶排出氧气。 一种充电适配器，其一侧可拆卸地安装在插入空间的开口中，当柱阀关闭路径时，将从外部供给的氧气充入氧气瓶中，并且当柱子在柱子上时通过排出口排出氧气瓶的氧气 阀打开路径。

公开(公告)号：US20100051951A1

公开(公告)日：2010-03-04

申请号：US12414932

申请日：2009-03-31

Applicant: Kwang-Ho Lee ,  Jang-Soo Kim ,  Hong-Suk Yoo ,  Sang-Soo Kim ,  Shi-Yul Kim ,  Jae-Hyoung Youn

Inventor： Kwang-Ho Lee ,  Jang-Soo Kim ,  Hong-Suk Yoo ,  Sang-Soo Kim ,  Shi-Yul Kim ,  Jae-Hyoung Youn

IPC: H01L33/00 ,  H01L21/28

CPC classification number: H01L27/124 ,  G02F1/13394 ,  G02F1/136209 ,  G02F1/136227 ,  G02F2001/136222 ,  H01L27/1248 ,  H01L27/1262

Abstract: A thin film transistor (“TFT”) array panel includes; an insulation substrate, a TFT disposed on the insulation substrate and including a drain electrode, a passivation layer covering the TFT and including a contact portion disposed therein corresponding to the drain electrode, a partition comprising an organic material disposed on the passivation layer, and including a transverse portion, a longitudinal portion, and a contact portion disposed on the drain electrode, a color filter disposed on the passivation layer and disposed in a region defined by the partition, an organic capping layer disposed on the partition and the color filter, and a pixel electrode disposed on the organic capping layer, and connected to the drain electrode through the contact portion of the passivation layer and the contact portion of the partition, wherein a contact hole is formed in the organic capping layer corresponding to the contact portion of the passivation layer.

Abstract translation: 薄膜晶体管（“TFT”）阵列面板包括： 绝缘基板，设置在所述绝缘基板上并包括漏电极的TFT，覆盖所述TFT的钝化层，并且包括设置在其中的所述漏电极对应的接触部分，所述隔板包括设置在所述钝化层上的有机材料，并且包括 横向部分，纵向部分和设置在漏电极上的接触部分，设置在钝化层上并设置在由隔板限定的区域中的滤色器，设置在隔板上的有机覆盖层和滤色器，以及 设置在所述有机覆盖层上的像素电极，并且通过所述钝化层的接触部分和所述隔离物的接触部分连接到所述漏电极，其中在所述有机覆盖层中形成接触孔，所述接触孔对应于所述钝化层的接触部分 钝化层。

公开(公告)号：US20090053779A1

公开(公告)日：2009-02-26

申请号：US12097099

申请日：2007-03-14

Applicant: Sang Yup Lee ,  Jin Hwan Park ,  Kwang Ho Lee ,  Tae Yong Kim

Inventor： Sang Yup Lee ,  Jin Hwan Park ,  Kwang Ho Lee ,  Tae Yong Kim

IPC: C12P13/08 ,  C12N15/01 ,  C12N15/80 ,  C12N15/81 ,  C12N15/74 ,  C12N1/14 ,  C12N1/15 ,  C12N1/16 ,  C12N1/19 ,  C12N1/20 ,  C12N1/21

CPC classification number: C12P13/08 ,  C12N9/1014 ,  C12Y201/02011

Abstract: The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site-specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine.

Abstract translation: 本发明涉及具有提高的支链氨基酸生产率的突变微生物，以及使用突变微生物生产支链氨基酸的方法。 更具体地，涉及通过减弱或缺失编码参与L-异亮氨酸生物合成的酶的基因，编码L-亮氨酸的酶的基因和编码L-亮氨酸的基因而产生的L-缬氨酸的生产率提高的突变体微生物 涉及D-泛酸生物合成的酶，以及编码参与L-缬氨酸生物合成的酶的基因的突变，使得其表达增加，以及使用突变微生物产生L-缬氨酸的方法。 通过位点特异性诱变和代谢途径工程产生的本发明的突变体微生物可以高效地产生支链氨基酸，特别是L-缬氨酸，因此可用作生产L-缬氨酸的工业微生物。