公开(公告)号：US06911333B2

公开(公告)日：2005-06-28

申请号：US10401436

申请日：2003-03-26

申请人： David A. Estell

发明人： David A. Estell

IPC分类号： C11D3/386 ,  C12N1/21 ,  C12N9/18 ,  C12N9/48 ,  C12N9/56 ,  C12N15/75 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00 ,  C12P21/04

CPC分类号： C12N9/54 ,  C11D3/386 ,  C11D3/38663 ,  C12N9/18 ,  C12N9/48 ,  C12N15/75

摘要： The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.

公开(公告)号：US06881562B2

公开(公告)日：2005-04-19

申请号：US10402067

申请日：2003-03-26

申请人： David A. Estell

发明人： David A. Estell

IPC分类号： C11D3/386 ,  C12N1/21 ,  C12N9/18 ,  C12N9/48 ,  C12N9/56 ,  C12N15/75 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00 ,  C12P21/04

CPC分类号： C12N9/54 ,  C11D3/386 ,  C11D3/38663 ,  C12N9/18 ,  C12N9/48 ,  C12N15/75

摘要： The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.

摘要翻译： 本发明涉及革兰氏阳性微生物中新型丝氨酸蛋白酶的鉴定。 本发明提供枯草芽孢杆菌丝氨酸蛋白酶SP1，SP2，SP3，SP4和SP5的核酸和氨基酸序列。 本发明还提供具有编码SP1，SP2，SP3，SP4和SP5的基因的部分或全部的突变或缺失的宿主细胞。 本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了包含本发明的丝氨酸蛋白酶的清洁组合物。

公开(公告)号：US06835550B1

公开(公告)日：2004-12-28

申请号：US09060872

申请日：1998-04-15

申请人： David A. Estell ,  Fiona A. Harding

发明人： David A. Estell ,  Fiona A. Harding

IPC分类号： C12N500

CPC分类号： G01N33/6878 ,  C12N9/54 ,  G01N33/505

摘要： The present invention relates to a novel improved protein mutant which produces low allergenic response in humans compared to the parent of that mutant. Specifically, the present invention comprises neutralizing or reducing the ability of T-cells to recognize epitopes and thus prevent sensitization of an individual to the protein.

摘要翻译： 本发明涉及与该突变体的亲本相比，在人类中产生低变应原反应的新型改良蛋白质突变体。 具体地，本发明包括中和或降低T细胞识别表位的能力，从而防止个体对蛋白质的致敏。

公开(公告)号：US06833265B2

公开(公告)日：2004-12-21

申请号：US09932183

申请日：2001-08-17

申请人： David A. Estell

发明人： David A. Estell

IPC分类号： C12N1575

CPC分类号： C12N9/54 ,  A23K20/189 ,  C11D3/386 ,  C12N15/75 ,  D06M16/003

摘要： The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus (MP). The present invention also provides host cells having mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.

摘要翻译： 本发明涉及革兰阳性微生物中新型金属蛋白酶（MP）的鉴定。 本发明提供芽孢杆菌（MP）的核酸和氨基酸序列。 本发明还提供具有编码MP的基因的部分或全部的突变或缺失的宿主细胞。 本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了包含本发明的MP的清洁组合物。

公开(公告)号：US06596525B1

公开(公告)日：2003-07-22

申请号：US09255501

申请日：1999-02-23

申请人： David A. Estell ,  Fiona A. Harding

发明人： David A. Estell ,  Fiona A. Harding

IPC分类号： A61K3848

CPC分类号： G01N33/6878 ,  C12N9/54 ,  G01N33/505

摘要： The present invention relates to a novel improved protein mutant which produces low allergenic response in humans compared to the parent of that mutant. Specifically, the present invention comprises neutralizing or reducing the ability of T-cells to recognize epitopes and thus prevent sensitization of an individual to the protein.

摘要翻译： 本发明涉及与该突变体的亲本相比，在人类中产生低变应原反应的新型改良蛋白质突变体。 具体地，本发明包括中和或降低T细胞识别表位的能力，从而防止个体对蛋白质的致敏。

公开(公告)号：US06316241B1

公开(公告)日：2001-11-13

申请号：US09355166

申请日：1999-07-20

申请人： David A. Estell

发明人： David A. Estell

IPC分类号： C12N950

CPC分类号： C11D3/386 ,  A23K20/189 ,  C11D3/38 ,  C12N9/14

摘要： The present invention relates to the identification of novel hydrolases in gram positive microorganisms. The present invention provides amino acid sequences for the hydrolase. The present invention provides host cells which comprise nucleic acid encoding the hydrolase. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the hydrolase of the present invention.

摘要翻译： 本发明涉及革兰氏阳性微生物中新型水解酶的鉴定。 本发明提供水解酶的氨基酸序列。 本发明提供了包含编码水解酶的核酸的宿主细胞。 本发明还提供了用于处理包括本发明的水解酶的纺织品的清洁组合物，动物饲料和组合物。

公开(公告)号：US5441882A

公开(公告)日：1995-08-15

申请号：US521010

申请日：1990-05-09

申请人： David A. Estell ,  James A. Wells ,  Richard R. Bott

发明人： David A. Estell ,  James A. Wells ,  Richard R. Bott

IPC分类号： C11D3/386 ,  C12N9/54 ,  C12N15/10 ,  C12N15/75 ,  C12R1/07 ,  C12N15/00 ,  C12N15/57

CPC分类号： C12R1/07 ,  C11D3/386 ,  C12N15/102 ,  C12N15/75 ,  C12N9/54

摘要： There are described methods for making mutant subtilisins, the methods comprising obtaining a DNA fragment from a Bacillus subtilisin and introducing a mutation into the fragment by substituting at least one amino acid, transforming a suitable host cell with the mutated DNA, recovering a mutant subtilisin and screening the mutant subtilisin for certain altered enzymatic properties.

摘要翻译： 描述了制备突变型枯草杆菌蛋白酶的方法，所述方法包括从枯草杆菌蛋白酶获得DNA片段，并通过用至少一个氨基酸取代突变进入该片段，用突变的DNA转化合适的宿主细胞，回收突变型枯草杆菌蛋白酶和 筛选突变的枯草杆菌蛋白酶以获得某些改变的酶特性。

公开(公告)号：US5310675A

公开(公告)日：1994-05-10

申请号：US805605

申请日：1991-12-10

申请人： David A. Estell ,  Eugenio Ferrari ,  Dennis J. Henner ,  James A. Wells

发明人： David A. Estell ,  Eugenio Ferrari ,  Dennis J. Henner ,  James A. Wells

IPC分类号： C11D3/386 ,  C12N9/54 ,  C12N15/10 ,  C12N15/75 ,  C12N15/52

CPC分类号： C12R1/07 ,  C11D3/386 ,  C12N15/102 ,  C12N15/75 ,  C12N9/54

摘要： Recombinant host bacteria and plasmids for making the bacteria using recombinant DNA techniques are described. The plasmids contain DNA coding for subtilisin with an amino acid substitution. Expression of the plasmid DNA results in production of a modified subtilisin.

摘要翻译： 描述了使用重组DNA技术制备细菌的重组宿主细菌和质粒。 质粒含有编码具有氨基酸取代的枯草杆菌蛋白酶的DNA。 质粒DNA的表达导致修饰的枯草杆菌蛋白酶的产生。

公开(公告)号：US5204015A

公开(公告)日：1993-04-20

申请号：US958885

申请日：1992-10-09

申请人： Robert M. Caldwell ,  David A. Estell ,  Thomas P. Graycar

发明人： Robert M. Caldwell ,  David A. Estell ,  Thomas P. Graycar

IPC分类号： C11D3/386 ,  C12N9/54 ,  C12N9/56 ,  C12N15/10 ,  C12N15/75

CPC分类号： C12R1/07 ,  C11D3/386 ,  C12N15/102 ,  C12N15/75 ,  C12N9/54

摘要： Novel carbonyl hydrolase mutants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The mutant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of one or more amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such mutant carbonyl hydrolases have properties which are different from those of the precursor hydrolase and are especially useful in detergent formulations. The substituted amino acid residues correspond to position +123 and/or +274 in Bacillus amyloliquefaciens subtilisin.

公开(公告)号：US09084734B2

公开(公告)日：2015-07-21

申请号：US11919717

申请日：2006-04-25

申请人： Katherine D. Collier ,  Anthony Day ,  Hans de Nobel ,  David A. Estell ,  Grant C. Ganshaw ,  Marc Kolkman ,  Raj Lad ,  Jeffrey V. Miller ,  Christopher J. Murray ,  Scott D. Power ,  Brian Schmidt ,  Anita van Kimmenade ,  Gudrun Vogtentanz

发明人： Katherine D. Collier ,  Anthony Day ,  Hans de Nobel ,  David A. Estell ,  Grant C. Ganshaw ,  Marc Kolkman ,  Raj Lad ,  Jeffrey V. Miller ,  Christopher J. Murray ,  Scott D. Power ,  Brian Schmidt ,  Anita van Kimmenade ,  Gudrun Vogtentanz

IPC分类号： A61K8/02 ,  A61K8/64 ,  A61K8/66 ,  A61Q1/02 ,  A61Q1/04 ,  A61Q1/10 ,  A61Q7/02 ,  A61Q9/00 ,  A61Q15/00 ,  A61Q17/04 ,  A61Q19/02 ,  A61Q19/04 ,  A61Q19/10 ,  C07K7/06

CPC分类号： A61K8/64 ,  A61K8/66 ,  A61Q1/02 ,  A61Q1/04 ,  A61Q1/10 ,  A61Q7/02 ,  A61Q9/00 ,  A61Q15/00 ,  A61Q17/04 ,  A61Q19/00 ,  A61Q19/02 ,  A61Q19/04 ,  A61Q19/10 ,  C07K7/06

摘要： The present invention provides peptides and supported peptides for treating various diseases and conditions. In particularly preferred embodiments, the present invention provides compositions and methods for personal care. In some embodiments the present invention provides compositions for use in skin and/or hair care, as well as cosmetic compositions. IN alternative particularly preferred embodiments, the present invention provides peptides and supported peptides for treating diseases of the skin, such as rosacea. In some particularly preferred embodiments, the supported peptides of the present invention are anti-VEGF peptides. In alternative particularly preferred embodiments, the anti-VEGF peptides are expressed on a scaffold protein. In some most preferred embodiments, the scaffold protein comprises BBI.

摘要翻译： 本发明提供用于治疗各种疾病和病症的肽和负载肽。 在特别优选的实施方案中，本发明提供用于个人护理的组合物和方法。 在一些实施方案中，本发明提供用于皮肤和/或毛发护理以及化妆品组合物的组合物。 在替代的特别优选的实施方案中，本发明提供用于治疗皮肤疾病的肽和负载的肽，例如红斑痤疮。 在一些特别优选的实施方案中，本发明的负载型肽是抗VEGF肽。 在替代的特别优选的实施方案中，抗VEGF肽在支架蛋白上表达。 在一些最优选的实施方案中，支架蛋白包含BBI。