公开(公告)号：US20200032227A1

公开(公告)日：2020-01-30

申请号：US16514578

申请日：2019-07-17

申请人： Codexis, Inc. ,  Tate & Lyle Ingredients Americas LLC

发明人： Jonathan Vroom ,  Stephanie Sue Galanie ,  Jack Liang ,  Joyce Liu ,  Nikki Dellas ,  Melissa Ann Mayo ,  David Entwistle

IPC分类号： C12N9/10 ,  C12P19/56 ,  C07H15/252

摘要： The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.

公开(公告)号：US20200017845A1

公开(公告)日：2020-01-16

申请号：US16441458

申请日：2019-06-14

申请人： Codexis, Inc.

发明人： Scott J. Novick ,  Ravi David Garcia ,  Charlene Ching ,  Nikki Dellas ,  David Entwistle ,  Vesna Mitchell

IPC分类号： C12N9/88

摘要： The present invention provides engineered phenylalanine ammonia lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia lyase (PAL) polypeptides. Methods for producing PAL enzymes are also provided. In some embodiments, the engineered PAL polypeptides are optimized to provide enhanced catalytic activities that are useful under industrial process conditions for the production of pharmaceutical compounds.

公开(公告)号：US20180187180A1

公开(公告)日：2018-07-05

申请号：US15861849

申请日：2018-01-04

申请人： Codexis, Inc.

发明人： Oscar Alvizo ,  David Elgart ,  Robert Kevin Orr ,  James Nicholas Riggins ,  Anna Fryszkowska ,  Katrina W. Lexa ,  Xiang Yi ,  Da Duan ,  Courtney Dianne Moffett ,  Nikki Dellas ,  Vesna Mitchell

IPC分类号： C12N9/84 ,  C12P21/02 ,  C07K14/62

摘要： The present invention provides engineered penicillin G acylase (PGA) enzymes, polynucleotides encoding the enzymes, compositions comprising the enzymes, and methods of using the engineered PGA enzymes.

公开(公告)号：US20170321202A1

公开(公告)日：2017-11-09

申请号：US15588378

申请日：2017-05-05

申请人： Codexis, Inc.

发明人： Jovana Nazor ,  Vesna Mitchell ,  David Elgart ,  Katrina W. Lexa ,  Nikki Dellas ,  Robert Kevin Orr ,  Oscar Alvizo ,  Ravi David Garcia ,  Judy Victoria Antonio Viduya ,  Courtney Dianne Moffett

IPC分类号： C12N9/84 ,  C12P21/02

摘要： The present invention provides engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.