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    ENGINEERED GLYCOSYLTRANSFERASES AND STEVIOL GLYCOSIDE GLUCOSYLATION METHODS
    1.
    发明申请
    ENGINEERED GLYCOSYLTRANSFERASES AND STEVIOL GLYCOSIDE GLUCOSYLATION METHODS 审中-公开

    公开(公告)号：US20200032227A1

    公开(公告)日：2020-01-30

    申请号：US16514578

    申请日：2019-07-17

    申请人： Codexis, Inc. ,  Tate & Lyle Ingredients Americas LLC

    发明人： Jonathan Vroom ,  Stephanie Sue Galanie ,  Jack Liang ,  Joyce Liu ,  Nikki Dellas ,  Melissa Ann Mayo ,  David Entwistle

    IPC分类号： C12N9/10 ,  C12P19/56 ,  C07H15/252

    摘要： The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.

    ENGINEERED GLYCOSYLTRANSFERASES AND STEVIOL GLYCOSIDE GLUCOSYLATION METHODS
    7.
    发明申请
    ENGINEERED GLYCOSYLTRANSFERASES AND STEVIOL GLYCOSIDE GLUCOSYLATION METHODS 有权

    公开(公告)号：US20210054348A1

    公开(公告)日：2021-02-25

    申请号：US17081904

    申请日：2020-10-27

    申请人： Codexis, Inc.

    发明人： Jonathan Vroom ,  Stephanie Sue Galanie ,  Nikki Dellas ,  Jack Liang ,  Joyce Liu ,  David Entwistle ,  Courtney Dianne Moffett

    IPC分类号： C12N9/10 ,  C12P19/18 ,  C12P19/56 ,  C12N9/88

    摘要： The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.