公开(公告)号：US07892784B2

公开(公告)日：2011-02-22

申请号：US11710612

申请日：2007-02-26

申请人： Hiroshi Takagi ,  Yoshimitsu Hamano

发明人： Hiroshi Takagi ,  Yoshimitsu Hamano

IPC分类号： C12P1/00 ,  C12P17/00 ,  C12P17/16 ,  C12P15/00

CPC分类号： C12N9/86 ,  C12P19/28

摘要： An enzyme capable of reducing the antibiotic activity (i.e., toxicity) of streptothricin with respect to eukaryotic cells without sacrificing antibiotic activity with respect to prokaryotic cells is provided along with a streptothricin derivative having reduced toxicity with respect to eukaryotic cells while retaining antibiotic activity with respect to prokaryotic cells, and a manufacturing method thereof. By opening the lactam ring of streptothricin, a protein having the amino acid sequence according to SEQ ID NO: 2, 4, 6, 8, 10, 12, 14 or 16 of the invention can reduce the antibiotic activity of streptothricin D with respect to eukaryotic cells without sacrificing antibiotic activity with respect to prokaryotic cells.

摘要翻译： 提供能够降低链霉素相对于真核细胞的抗生素活性（即毒性）而不牺牲相对于原核细胞的抗生素活性的酶以及相对于真核细胞毒性降低的链球菌素衍生物，同时保持抗生素活性 原核细胞及其制造方法。 通过打开链霉素的内酰胺环，具有根据本发明的SEQ ID NO：2,4,6,8,10,12,14或16的氨基酸序列的蛋白质可以降低链球菌D的抗生素活性，相对于 真核细胞，而不牺牲相对于原核细胞的抗生素活性。

公开(公告)号：US20100093045A1

公开(公告)日：2010-04-15

申请号：US12635404

申请日：2009-12-10

申请人： Hiroshi Takagi ,  Shigeru Nakamori ,  Masaru Wada ,  Hirotada Mori

发明人： Hiroshi Takagi ,  Shigeru Nakamori ,  Masaru Wada ,  Hirotada Mori

IPC分类号： C12P13/12

CPC分类号： C12P13/12

摘要： L-cysteine is produced by culturing an Escherichia bacterium having L-cysteine producing ability and containing a gene encoding an O-acetylserine sulphydrylase B or MalY regulatory protein that is modified so that cysteine desulfhydrase activity is reduced or eliminated. The bacterium is cultured in a medium to produce and cause accumulation of L-cysteine in the medium, and collecting L-cysteine from the medium.

摘要翻译： 通过培养具有L-半胱氨酸生产能力的埃希氏菌属细菌并含有编码O-乙酰丝氨酸巯基酶B或MalY调节蛋白的基因来产生L-半胱氨酸，所述基因被修饰以使得半胱氨酸脱硫酸酶活性被降低或消除。 将细菌培养在培养基中以产生并引起培养基中L-半胱氨酸的积累，并从培养基中收集L-半胱氨酸。

公开(公告)号：US20080076163A1

公开(公告)日：2008-03-27

申请号：US11070084

申请日：2005-03-03

申请人： Hiroshi Takagi ,  Shigeru Nakamori ,  Masaru Wada ,  Hirotada Mori

发明人： Hiroshi Takagi ,  Shigeru Nakamori ,  Masaru Wada ,  Hirotada Mori

IPC分类号： C12P13/12 ,  C12N1/21

CPC分类号： C12P13/12

摘要： L-cysteine is produced by culturing an Escherichia bacterium having L-cysteine producing ability and containing a gene encoding an O-acetylserine sulphydrylase B or MalY regulatory protein that is modified so that cysteine desulfhydrase activity is reduced or eliminated. The bacterium is cultured in a medium to produce and cause accumulation of L-cysteine in the medium, and collecting L-cysteine from the medium.

摘要翻译： 通过培养具有L-半胱氨酸生产能力的埃希氏菌属细菌并含有编码O-乙酰丝氨酸巯基酶B或MalY调节蛋白的基因来产生L-半胱氨酸，所述基因被修饰以使得半胱氨酸脱硫酸酶活性被降低或消除。 将细菌培养在培养基中以产生并引起培养基中L-半胱氨酸的积累，并从培养基中收集L-半胱氨酸。

公开(公告)号：US07332259B2

公开(公告)日：2008-02-19

申请号：US11102311

申请日：2005-04-08

申请人： Hiroshi Takagi

发明人： Hiroshi Takagi

IPC分类号： G03F7/00

CPC分类号： B41N3/038 ,  B41C1/1008 ,  B41C1/1016 ,  B41C1/1025 ,  B41C2201/02 ,  B41C2201/12 ,  B41C2201/14 ,  B41C2210/04 ,  B41C2210/06 ,  B41C2210/22 ,  B41C2210/24

摘要： Disclosed is a process for manufacturing an aluminum support for a light sensitive planographic printing plate material, the process comprising the steps of surface-roughening an aluminum plate, anodizing the surface-roughened aluminum plate, surface treating the anodized aluminum plate with an aqueous polyvinyl phosphonic acid solution, and drying the resulting plate at from 150 to 230° C. to obtain the aluminum support.

摘要翻译： 公开了一种用于制造光敏平版印刷版材料的铝支架的方法，该方法包括以下步骤：对铝板进行表面粗糙化，阳极氧化表面粗糙化的铝板，用阳离子化铝板表面处理聚乙烯基膦酸盐 酸溶液，并将所得板在150-230℃下干燥，得到铝载体。

公开(公告)号：US07329726B2

公开(公告)日：2008-02-12

申请号：US11010800

申请日：2004-12-13

申请人： Shigeru Nakamori ,  Hiroshi Takagi ,  Masakazu Takahashi ,  Kazuhisa Tsujimoto ,  Hideyuki Yamada

发明人： Shigeru Nakamori ,  Hiroshi Takagi ,  Masakazu Takahashi ,  Kazuhisa Tsujimoto ,  Hideyuki Yamada

IPC分类号： C07K7/06 ,  A61K38/08 ,  C12N5/02

CPC分类号： C07K14/43586 ,  C12N5/0018 ,  C12N5/0601 ,  C12N2501/998 ,  C12N2501/999

摘要： A peptide according to the present invention is selected from the group consisting of: (a) a peptide having the amino acid sequence represented by SEQ ID NO: 1; (b) a peptide having a modified amino acid sequence of the amino acid sequence described in (a) above, in which one or more amino acid residues are deleted, substituted, inserted or added, and having cell death-preventing activity and/or cell growth-promoting activity; and (c) a peptide which has an amino acid sequence having at least 80% homology with the peptide consisting of the amino acid sequence described in (a) above and has cell death-preventing activity and/or cell growth-promoting activity. The peptide has cell death-preventing activity and/or cell growth-promoting activity, is highly safe, and can be produced without difficulty. The peptide is useful as a culture supplement or as an effective component for a cell culture medium.

摘要翻译： 根据本发明的肽选自：（a）具有由SEQ ID NO：1表示的氨基酸序列的肽; （b）具有上述（a）中所述的氨基酸序列的修饰氨基酸序列的肽，其中一个或多个氨基酸残基被缺失，取代，插入或添加并具有细胞死亡预防活性和/或 细胞生长促进活性; 和（c）具有与由上述（a）中所述的氨基酸序列组成的肽具有至少80％同源性的氨基酸序列并具有细胞死亡预防活性和/或细胞生长促进活性的肽。 肽具有细胞死亡预防活性和/或细胞生长促进活性，是高度安全的，可以毫无困难地制备。 该肽可用作培养物补充物或作为细胞培养基的有效成分。

公开(公告)号：US20060037506A1

公开(公告)日：2006-02-23

申请号：US11187398

申请日：2005-07-22

申请人： Hiroshi Takagi

发明人： Hiroshi Takagi

IPC分类号： B41N1/00

CPC分类号： B41C1/1008 ,  B41C1/1016 ,  B41C2201/02 ,  B41C2201/12 ,  B41C2201/14 ,  B41C2210/02 ,  B41C2210/04 ,  B41C2210/22 ,  B41C2210/24 ,  B41N1/083 ,  B41N3/034

摘要： An object of the invention is to provide a planographic printing plate material exhibiting excellent dot reproduction and printing durability and a support for the planographic printing plate material. Disclosed is a support for a planographic printing plate material having a surface roughened via electrolytic surface-roughening treatment and anodization treatment conducted to one surface of an aluminum plate, wherein a) average surface roughness (Ra) of the roughened surface is 0.30-0.55 μm, and b) the roughened surface possesses a surface profile in which a ratio of Xa/Xb is 0.40-0.70, where Xa is the width expanding to the shallow region side and Xb is the width expanding to the deep region side at the position reaching peak depth in amplitude frequency.

摘要翻译： 本发明的目的是提供一种平版印刷版材料，其具有优异的点再现性和印刷耐久性，并且对平版印刷版材料的支持。 公开了通过经由电解表面粗糙化处理进行表面粗糙化并对铝板的一个表面进行阳极氧化处理的平版印刷版材的支撑体，其中，a）粗糙面的平均表面粗糙度（Ra）为0.30〜0.55μm ，b）粗糙表面具有Xa / Xb的比例为0.40-0.70的表面轮廓，其中Xa是向浅区域侧扩展的宽度，Xb是在到达的位置处扩展到深区域侧的宽度 振幅频率峰值深度。

公开(公告)号：US20050233250A1

公开(公告)日：2005-10-20

申请号：US11102311

申请日：2005-04-08

申请人： Hiroshi Takagi

发明人： Hiroshi Takagi

IPC分类号： G03F7/09 ,  B41C1/10 ,  B41N1/08 ,  B41N3/03 ,  G03F7/00

CPC分类号： B41N3/038 ,  B41C1/1008 ,  B41C1/1016 ,  B41C1/1025 ,  B41C2201/02 ,  B41C2201/12 ,  B41C2201/14 ,  B41C2210/04 ,  B41C2210/06 ,  B41C2210/22 ,  B41C2210/24

摘要： Disclosed is a process for manufacturing an aluminum support for a light sensitive planographic printing plate material, the process comprising the steps of surface-roughening an aluminum plate, anodizing the surface-roughened aluminum plate, surface treating the anodized aluminum plate with an aqueous polyvinyl phosphonic acid solution, and drying the resulting plate at from 150 to 230 ° C. to obtain the aluminum support.

摘要翻译： 公开了一种用于制造光敏平版印刷版材料的铝支架的方法，该方法包括以下步骤：对铝板进行表面粗糙化，阳极氧化表面粗糙化的铝板，用阳离子化铝板表面处理聚乙烯基膦酸盐 酸溶液，并将所得板在150-230℃下干燥，得到铝载体。

公开(公告)号：US20050221453A1

公开(公告)日：2005-10-06

申请号：US10957828

申请日：2004-10-05

申请人： Hiroshi Takagi ,  Shigeru Nakamori ,  Akihito Yamaguchi ,  Kunihiko Nishino

发明人： Hiroshi Takagi ,  Shigeru Nakamori ,  Akihito Yamaguchi ,  Kunihiko Nishino

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12P13/06 ,  C12P13/12

CPC分类号： C12P13/12 ,  C12P13/06

摘要： L-Cysteine is produced by culturing a microorganism having an ability to produce L-cysteine and modified so that expression of emrAB, emrKY, yojIH, acrEF, bcr, or cusA gene should be enhanced in a medium to produce and accumulate L-cysteine in the medium and collecting the L-cysteine from the medium. Genes coding for novel L-cysteine-excreting proteins are identified, and utilized for breeding of L-cysteine-producing microorganism to provide a novel method of producing L-cysteine.

摘要翻译： L-半胱氨酸通过培养具有产生L-半胱氨酸的能力的微生物并进行修饰以使得在培养基中增强emrAB，emrKY，yojIH，acrEF，bcr或cusA基因的表达以产生和积累L-半胱氨酸来产生L-半胱氨酸 培养基并从培养基中收集L-半胱氨酸。 鉴定出编码新型L-半胱氨酸分泌蛋白的基因，用于生产L-半胱氨酸的微生物的育种，以提供L-半胱氨酸的新方法。

公开(公告)号：US06891963B1

公开(公告)日：2005-05-10

申请号：US09869760

申请日：2000-01-05

申请人： Yoshihiro Goto ,  Hiroshi Takagi ,  Dai Shinohara

发明人： Yoshihiro Goto ,  Hiroshi Takagi ,  Dai Shinohara

IPC分类号： A61B6/03 ,  G06Q50/22 ,  G06Q50/24 ,  G06T1/00 ,  G06T11/00 ,  G06T11/80 ,  G06T15/00 ,  G06K9/00

CPC分类号： A61B6/032 ,  A61B6/4085 ,  A61B6/463 ,  A61B6/466 ,  A61B6/5223 ,  G06T15/08

摘要： In an image display apparatus capable of storing therein a small amount of images and also capable of displaying two sorts, or more of images within a short time period, an axial image 1, a sagittal image 2, and a coronal image 3, which are loaded from a magnetic disk, are displayed on a CRT monitor 117. Image position indication FIGS. 4, 5, 6 are indicated above the respective tomographic images, and positions of the respective tomographic images are indicated on the respective image position indication FIGS. 4, 5, 6 by employing heavy lines 4a, 5a, 6a, and triangular symbols 4b, 5b, 6b. The positions of the tomographic images can be adjusted by dragging the respective triangular symbols 4b, 5b, 6b by using a mouse 118. Image position input FIGS. 7, 8, 9 are indicated under the respective tomographic images, and the positions of the respective displayed tomographic images are indicated on the image position input FIGS. 7, 8, 9 by way of drag bars 7a, 8a, 9a. Alternatively, the positions of the tomographic images may be adjusted by dragging the drag bars 7a, 8a, 9a by using the mouse 118.

摘要翻译： 在能够在其中存储少量图像并且还能够在短时间内显示两种或多种图像的图像显示装置中，轴图像1，矢状图像2和冠状图像3是 显示在CRT显示器117上。 图像位置指示 在相应的断层图像上方表示4,5,6，并且在相应的图像位置指示图中指示各个断层图像的位置。 通过使用重线4a，5a，6a和三角形符号4b，5b，6b，4,5,6。 可以通过使用鼠标118拖动相应的三角形符号4b，5b，6b来调整断层图像的位置。 图像位置输入 在各断层图像下表示7,8,9，各显示的断层摄影图像的位置被指示在图像位置输入图中。 如图7,8,9所示，通过拖杆7a，8a，9a。 或者，可以通过使用鼠标118拖动牵引杆7a，9a，9a来调节断层图像的位置。

公开(公告)号：US06616631B2

公开(公告)日：2003-09-09

申请号：US10089707

申请日：2002-03-29

申请人： Hiroshi Takagi ,  Kentaro Takemae

发明人： Hiroshi Takagi ,  Kentaro Takemae

IPC分类号： A61M500

CPC分类号： A61M25/0631

摘要： A holder sleeve (1) incorporates an urging means (8) for urging an inner needle (12) to the proximal end side and an actuator (6) which engages the inner needle (12) at the near distal end portion of the holder sleeve (1) so that the inner needle (12) can be released and the tip of the inner needle is projected from the holder sleeve (1). This actuator (6) has an inner needle hub (12a) fixed at the front end thereof and is formed with an actuating member (6a) projected outside the holder sleeve (1). The holder sleeve (1) has a slit (2) which extends to the proximal end side so as to allow the actuating member (6a) of the actuator (6) to slide and assures the path of movement of the actuator (6) released from the near distal end portion of the holder sleeve (1). The inner needle (12) is adapted to be released from the holder sleeve (1) when the actuating member (6a) is moved away from the holder sleeve (1) within the same plane that includes the axis of the holder sleeve (1).