公开(公告)号：US20050196861A1

公开(公告)日：2005-09-08

申请号：US11010800

申请日：2004-12-13

申请人： Shigeru Nakamori ,  Hiroshi Takagi ,  Masakazu Takahashi ,  Kazuhisa Tsujimoto ,  Hideyuki Yamada

发明人： Shigeru Nakamori ,  Hiroshi Takagi ,  Masakazu Takahashi ,  Kazuhisa Tsujimoto ,  Hideyuki Yamada

IPC分类号： C07K7/06 ,  C07K14/435 ,  C12N5/00 ,  C12N5/07 ,  A61K38/18 ,  C07K14/475 ,  C12N5/02

CPC分类号： C07K14/43586 ,  C12N5/0018 ,  C12N5/0601 ,  C12N2501/998 ,  C12N2501/999

摘要： A peptide according to the present invention is selected from the group consisting of: (a) a peptide having the amino acid sequence represented by SEQ ID NO: 1; (b) a peptide having a modified amino acid sequence of the amino acid sequence described in (a) above, in which one or more amino acid residues are deleted, substituted, inserted or added, and having cell death-preventing activity and/or cell growth-promoting activity; and (c) a peptide which has an amino acid sequence having at least 80% homology with the peptide consisting of the amino acid sequence described in (a) above and has cell death-preventing activity and/or cell growth-promoting activity. The peptide has cell death-preventing activity and/or cell growth-promoting activity, is highly safe, and can be produced without difficulty. The peptide is useful as a culture supplement or as an effective component for a cell culture medium.

摘要翻译： 根据本发明的肽选自：（a）具有由SEQ ID NO：1表示的氨基酸序列的肽; （b）具有上述（a）中所述的氨基酸序列的修饰氨基酸序列的肽，其中一个或多个氨基酸残基被缺失，取代，插入或添加并具有细胞死亡预防活性和/或 细胞生长促进活性; 和（c）具有与由上述（a）中所述的氨基酸序列组成的肽具有至少80％同源性的氨基酸序列并具有细胞死亡预防活性和/或细胞生长促进活性的肽。 肽具有细胞死亡预防活性和/或细胞生长促进活性，是高度安全的，可以毫无困难地制备。 该肽可用作培养物补充物或作为细胞培养基的有效成分。

公开(公告)号：US07314745B2

公开(公告)日：2008-01-01

申请号：US10469486

申请日：2002-03-01

申请人： Shigeru Nakamori ,  Hiroshi Takagi ,  Masakazu Takahashi ,  Kazuhisa Tsujimoto ,  Hideyuki Yamada

发明人： Shigeru Nakamori ,  Hiroshi Takagi ,  Masakazu Takahashi ,  Kazuhisa Tsujimoto ,  Hideyuki Yamada

IPC分类号： C12N9/64 ,  C12N15/57 ,  C12N15/79 ,  C12N15/85

CPC分类号： C12N9/64 ,  C12N9/6402

摘要： An objective of the present invention is to provide a new protein derived from the silkworm middle silk gland and a DNA encoding said protein. The present invention relates to a protein of the following (a) or (b): (a) a protein comprising the amino acid sequence represented by SEQ ID NO: 1; (b) a protein comprising a modified amino acid sequence of the amino acid sequence described in (a) above, in which one or more amino acid residues are deleted, substituted, inserted or added, and having protease activity.

摘要翻译： 本发明的目的是提供一种来自蚕丝绸的新蛋白质和编码所述蛋白质的DNA。 本发明涉及以下（a）或（b）的蛋白质：（a）包含SEQ ID NO：1所示的氨基酸序列的蛋白质; （b）包含上述（a）中描述的氨基酸序列的修饰氨基酸序列的蛋白质，其中一个或多个氨基酸残基被缺失，取代，插入或添加并具有蛋白酶活性。

公开(公告)号：US07329726B2

公开(公告)日：2008-02-12

申请号：US11010800

申请日：2004-12-13

申请人： Shigeru Nakamori ,  Hiroshi Takagi ,  Masakazu Takahashi ,  Kazuhisa Tsujimoto ,  Hideyuki Yamada

发明人： Shigeru Nakamori ,  Hiroshi Takagi ,  Masakazu Takahashi ,  Kazuhisa Tsujimoto ,  Hideyuki Yamada

IPC分类号： C07K7/06 ,  A61K38/08 ,  C12N5/02

CPC分类号： C07K14/43586 ,  C12N5/0018 ,  C12N5/0601 ,  C12N2501/998 ,  C12N2501/999

摘要： A peptide according to the present invention is selected from the group consisting of: (a) a peptide having the amino acid sequence represented by SEQ ID NO: 1; (b) a peptide having a modified amino acid sequence of the amino acid sequence described in (a) above, in which one or more amino acid residues are deleted, substituted, inserted or added, and having cell death-preventing activity and/or cell growth-promoting activity; and (c) a peptide which has an amino acid sequence having at least 80% homology with the peptide consisting of the amino acid sequence described in (a) above and has cell death-preventing activity and/or cell growth-promoting activity. The peptide has cell death-preventing activity and/or cell growth-promoting activity, is highly safe, and can be produced without difficulty. The peptide is useful as a culture supplement or as an effective component for a cell culture medium.

摘要翻译： 根据本发明的肽选自：（a）具有由SEQ ID NO：1表示的氨基酸序列的肽; （b）具有上述（a）中所述的氨基酸序列的修饰氨基酸序列的肽，其中一个或多个氨基酸残基被缺失，取代，插入或添加并具有细胞死亡预防活性和/或 细胞生长促进活性; 和（c）具有与由上述（a）中所述的氨基酸序列组成的肽具有至少80％同源性的氨基酸序列并具有细胞死亡预防活性和/或细胞生长促进活性的肽。 肽具有细胞死亡预防活性和/或细胞生长促进活性，是高度安全的，可以毫无困难地制备。 该肽可用作培养物补充物或作为细胞培养基的有效成分。

公开(公告)号：US4601983A

公开(公告)日：1986-07-22

申请号：US504471

申请日：1983-06-15

申请人： Shigeru Nakamori ,  Masaaki Ishida ,  Hiroshi Takagi ,  Kiyoshi Miwa ,  Koichi Ito ,  Konosuke Sano

发明人： Shigeru Nakamori ,  Masaaki Ishida ,  Hiroshi Takagi ,  Kiyoshi Miwa ,  Koichi Ito ,  Konosuke Sano

IPC分类号： C12N9/04 ,  C12N15/00 ,  C12N15/09 ,  C12N15/77 ,  C12P13/06 ,  C12P13/08 ,  C12R1/13 ,  C12R1/15 ,  C12R1/125

CPC分类号： C12N9/0006 ,  C12N15/77 ,  C12P13/06 ,  C12P13/08 ,  Y10S435/84 ,  Y10S435/843

摘要： A genetic sequence coding for the production of a protein having the activity of homoserine dehydrogenase and having two Pst I cleavage sites in its DNA chain and a molecular weight of 2.24 Md, is incorporated into a vehicle capable of replication in Coryneform bacteria and used to produce L-threonine and L-isoleucine by fermentation.

摘要翻译： 编码生产具有高丝氨酸脱氢酶活性并且在其DNA链中具有两个Pst I切割位点并且分子量为2.24Md的蛋白质的遗传序列被并入能够在棒状细菌中复制的载体中，并用于产生 L-苏氨酸和L-异亮氨酸通过发酵。

公开(公告)号：US08114649B2

公开(公告)日：2012-02-14

申请号：US12635404

申请日：2009-12-10

申请人： Hiroshi Takagi ,  Shigeru Nakamori ,  Masaru Wada ,  Hirotada Mori

发明人： Hiroshi Takagi ,  Shigeru Nakamori ,  Masaru Wada ,  Hirotada Mori

IPC分类号： C12N9/00 ,  C12N9/10 ,  C12P13/12 ,  C12N1/20

CPC分类号： C12P13/12

摘要： L-cysteine is produced by culturing an Escherichia bacterium having L-cysteine producing ability and containing a gene encoding an O-acetylserine sulphydrylase B or MalY regulatory protein that is modified so that cysteine desulfhydrase activity is reduced or eliminated. The bacterium is cultured in a medium to produce and cause accumulation of L-cysteine in the medium, and collecting L-cysteine from the medium.

摘要翻译： 通过培养具有L-半胱氨酸生产能力的埃希氏菌属细菌并含有编码O-乙酰丝氨酸巯基酶B或MalY调节蛋白的基因来产生L-半胱氨酸，所述基因被修饰以使得半胱氨酸脱硫酸酶活性被降低或消除。 将细菌培养在培养基中以产生并引起培养基中L-半胱氨酸的积累，并从培养基中收集L-半胱氨酸。

公开(公告)号：US07148047B2

公开(公告)日：2006-12-12

申请号：US10060218

申请日：2002-02-01

申请人： Hiroshi Takagi ,  Masaru Wada ,  Shigeru Nakamori

发明人： Hiroshi Takagi ,  Masaru Wada ,  Shigeru Nakamori

IPC分类号： C12N9/10 ,  C12N1/12 ,  C12P13/12 ,  C07K1/00 ,  C07H21/02

CPC分类号： C12P13/12

摘要： L-cysteine is produced by culturing a coryneform bacterium in which intracellular serine acetyltransferase activity is increased by transformation with a gene coding for serine acetyltransferase, such modification of an expression control sequence of a gene coding for serine acetyltransferase that expression of the gene in a cell should be enhanced and for forth, preferably in which L-cysteine decomposition system is further suppressed, in a medium to produce and accumulate L-cysteine in culture and collecting the L-cysteine from the culture.

公开(公告)号：US06946268B2

公开(公告)日：2005-09-20

申请号：US10254763

申请日：2002-09-26

申请人： Hiroshi Takagi ,  Masaru Wada ,  Shigeru Nakamori

发明人： Hiroshi Takagi ,  Masaru Wada ,  Shigeru Nakamori

IPC分类号： C12N1/21 ,  C12P13/12 ,  C12R1/19 ,  C07H21/04 ,  C12N1/20 ,  C12N9/10 ,  C12N15/00

CPC分类号： C12R1/19 ,  C12N9/88 ,  C12P13/12

摘要： L-Cysteine is produced by culturing a bacterium belonging to the genus Escherichia having an L-cysteine producing ability and modified so that cystathionine-β-lyase activity or cystathionine-β-lyase activity and tryptophanase activity should be reduced or eliminated in a medium to produce and accumulate L-cysteine in the medium and collecting the L-cysteine from the medium.

摘要翻译： 通过培养具有L-半胱氨酸生产能力的埃希氏杆菌属细菌产生L-半胱氨酸，并进行修饰，以使得在培养基中减少或消除胱硫醚-β-裂解酶活性和色氨酸酶活性 在培养基中产生并积累L-半胱氨酸，并从培养基中收集L-半胱氨酸。

公开(公告)号：US20100093045A1

公开(公告)日：2010-04-15

申请号：US12635404

申请日：2009-12-10

申请人： Hiroshi Takagi ,  Shigeru Nakamori ,  Masaru Wada ,  Hirotada Mori

发明人： Hiroshi Takagi ,  Shigeru Nakamori ,  Masaru Wada ,  Hirotada Mori

IPC分类号： C12P13/12

CPC分类号： C12P13/12

摘要： L-cysteine is produced by culturing an Escherichia bacterium having L-cysteine producing ability and containing a gene encoding an O-acetylserine sulphydrylase B or MalY regulatory protein that is modified so that cysteine desulfhydrase activity is reduced or eliminated. The bacterium is cultured in a medium to produce and cause accumulation of L-cysteine in the medium, and collecting L-cysteine from the medium.

摘要翻译： 通过培养具有L-半胱氨酸生产能力的埃希氏菌属细菌并含有编码O-乙酰丝氨酸巯基酶B或MalY调节蛋白的基因来产生L-半胱氨酸，所述基因被修饰以使得半胱氨酸脱硫酸酶活性被降低或消除。 将细菌培养在培养基中以产生并引起培养基中L-半胱氨酸的积累，并从培养基中收集L-半胱氨酸。

公开(公告)号：US20080076163A1

公开(公告)日：2008-03-27

申请号：US11070084

申请日：2005-03-03

申请人： Hiroshi Takagi ,  Shigeru Nakamori ,  Masaru Wada ,  Hirotada Mori

发明人： Hiroshi Takagi ,  Shigeru Nakamori ,  Masaru Wada ,  Hirotada Mori

IPC分类号： C12P13/12 ,  C12N1/21

CPC分类号： C12P13/12

摘要： L-cysteine is produced by culturing an Escherichia bacterium having L-cysteine producing ability and containing a gene encoding an O-acetylserine sulphydrylase B or MalY regulatory protein that is modified so that cysteine desulfhydrase activity is reduced or eliminated. The bacterium is cultured in a medium to produce and cause accumulation of L-cysteine in the medium, and collecting L-cysteine from the medium.

摘要翻译： 通过培养具有L-半胱氨酸生产能力的埃希氏菌属细菌并含有编码O-乙酰丝氨酸巯基酶B或MalY调节蛋白的基因来产生L-半胱氨酸，所述基因被修饰以使得半胱氨酸脱硫酸酶活性被降低或消除。 将细菌培养在培养基中以产生并引起培养基中L-半胱氨酸的积累，并从培养基中收集L-半胱氨酸。