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公开(公告)号:US07253335B2
公开(公告)日:2007-08-07
申请号:US10916247
申请日:2004-08-11
申请人: Stephen M. Allen , Perry G. Caimi , Johan M. Stoop
发明人: Stephen M. Allen , Perry G. Caimi , Johan M. Stoop
CPC分类号: C12N9/1051 , C12N15/8246
摘要: This invention relates to isolated nucleic acid fragments encoding fructosyltransferases. More specifically, this invention relates to polynucleotides encoding 1-FFTs, 6-SFTs, or 1-SSTs. The invention also relates to the construction of a recombinant DNA constructs encoding all or a portion of the fructosyltransferases, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the fructosyltransferases in a transformed host cell.
摘要翻译: 本发明涉及编码果糖基转移酶的分离的核酸片段。 更具体地,本发明涉及编码1-FFT,6-SFT或1-SST的多核苷酸。 本发明还涉及在正义或反义方向上构建编码全部或一部分果糖基转移酶的重组DNA构建体,其中重组DNA构建体的表达导致转化宿主细胞中果糖基转移酶水平的变化。
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公开(公告)号:US07176009B2
公开(公告)日:2007-02-13
申请号:US10641991
申请日:2003-08-14
CPC分类号: C12N9/1066 , C12N15/8245 , C12Y204/01014
摘要: This invention relates to an isolated nucleic acid fragment encoding a sucrose phosphate synthase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the sucrose phosphate synthase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the sucrose phosphate synthase in a transformed host cell.
摘要翻译: 本发明涉及编码蔗糖磷酸合成酶的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或部分蔗糖磷酸合酶的嵌合基因,其中嵌合基因的表达导致在转化的宿主细胞中产生蔗糖磷酸合酶的改变水平 。
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公开(公告)号:US07022895B2
公开(公告)日:2006-04-04
申请号:US10734698
申请日:2003-12-12
申请人: Saverio Carl Falco , Stephen M. Allen , J. Antoni Rafalski , William D. Hitz , Anthony John Kinney , Lynn Marie Abell , Catherine J. Thorpe
发明人: Saverio Carl Falco , Stephen M. Allen , J. Antoni Rafalski , William D. Hitz , Anthony John Kinney , Lynn Marie Abell , Catherine J. Thorpe
IPC分类号: A01H1/00 , C07H21/04 , C07K14/415 , C12N5/14 , C12N9/00
CPC分类号: C12N9/001 , C12N9/1085 , C12N9/88 , C12N9/90 , C12N15/8251 , C12N15/8253 , C12N15/8254
摘要: This invention relates to an isolated nucleic acid fragment encoding a plant enzyme that catalyze steps in the biosynthesis of lysine, threonine, methionine, cysteine and isoleucine from aspartate, the enzyme a member selected from the group consisting of: dihydrodipicolinate reductase, diaminopimelate epimerase, threonine synthase, threonine deaminase and S-adenosylmethionine synthetase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the enzyme in a transformed host cell.
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公开(公告)号:US07002057B2
公开(公告)日:2006-02-21
申请号:US10978538
申请日:2004-11-01
申请人: Stephen M. Allen , John D. Everard
发明人: Stephen M. Allen , John D. Everard
CPC分类号: C12N9/0036 , C07K14/415 , C12N15/8241 , C12N15/8242
摘要: This invention relates to an isolated nucleic acid fragment encoding a thioredoxin protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the thioredoxin protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the thioredoxin protein in a transformed host cell.
摘要翻译: 本发明涉及编码硫氧还蛋白蛋白质的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或部分硫氧还蛋白蛋白质的嵌合基因,其中嵌合基因的表达导致转化宿主细胞中硫氧还蛋白蛋白质水平的变化。
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公开(公告)号:US06677502B1
公开(公告)日:2004-01-13
申请号:US09614912
申请日:2000-07-12
申请人: Stephen M. Allen , Anthony J. Kinney , J. Antoni Rafalski , Emil M. Orozco, Jr. , Guo-Hua Miao , Omolayo O. Famodu , Jian-Ming Lee , Karin N. Lohman , Alan R. Rendina , Hajime Sakai , Zude Weng , Perry G. Caimi , Yiwen Fang , Jennie Bih-Jien Shen , Ilham L. Zoughi , Shawn L. Anderson , Jinrui Shi , Guihua Lu , Timothy G. Helentjaris , Chun Ping Li
发明人: Stephen M. Allen , Anthony J. Kinney , J. Antoni Rafalski , Emil M. Orozco, Jr. , Guo-Hua Miao , Omolayo O. Famodu , Jian-Ming Lee , Karin N. Lohman , Alan R. Rendina , Hajime Sakai , Zude Weng , Perry G. Caimi , Yiwen Fang , Jennie Bih-Jien Shen , Ilham L. Zoughi , Shawn L. Anderson , Jinrui Shi , Guihua Lu , Timothy G. Helentjaris , Chun Ping Li
IPC分类号: A01H300
CPC分类号: C12N15/8297 , C12N9/78 , C12N15/8243 , C12Y305/04025
摘要: This invention relates to an isolated nucleic acid fragment encoding a GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate synthase protein. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the protein in a transformed host cell.
摘要翻译: 本发明涉及编码GTP环化水解酶II / 3,4-二羟基-2-丁酮-4-磷酸合成酶蛋白质的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码蛋白质的全部或大部分的嵌合基因,其中嵌合基因的表达导致转化宿主细胞中蛋白质水平的改变。
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公开(公告)号:US06525244B1
公开(公告)日:2003-02-25
申请号:US09433241
申请日:1999-11-04
IPC分类号: A01H300
CPC分类号: C12N9/1096
摘要: This invention relates to an isolated nucleic acid fragment encoding a histidine biosynthetic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the histidine biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the histidine biosynthetic enzyme in a transformed host cell.
摘要翻译: 本发明涉及编码组氨酸生物合成酶的分离的核酸片段。 本发明还涉及在正义或反义方向上编码编码全部或部分组氨酸生物合成酶的嵌合基因的构建,其中嵌合基因的表达导致转化的宿主细胞中组氨酸生物合成酶水平的改变 。
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公开(公告)号:US06441271B1
公开(公告)日:2002-08-27
申请号:US09425578
申请日:1999-10-22
申请人: Stephen M. Allen , William D. Hitz
发明人: Stephen M. Allen , William D. Hitz
IPC分类号: A01H100
CPC分类号: C12N15/8251 , C12N9/88
摘要: This invention relates to an isolated nucleic acid fragment encoding a histidine biosynthetic enzyme, imidazoleglycerol-phosphate dehydratase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the histidine biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the histidine biosynthetic enzyme in a transformed host cell.
摘要翻译: 本发明涉及编码组氨酸生物合成酶,咪唑甘油 - 磷酸脱水酶的分离的核酸片段。 本发明还涉及在正义或反义方向上编码编码全部或部分组氨酸生物合成酶的嵌合基因的构建,其中嵌合基因的表达导致转化的宿主细胞中组氨酸生物合成酶水平的改变 。
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公开(公告)号:US06262345B1
公开(公告)日:2001-07-17
申请号:US09347801
申请日:1999-07-02
申请人: Stephen M. Allen , Jian-Ming Lee
发明人: Stephen M. Allen , Jian-Ming Lee
IPC分类号: A01H900
CPC分类号: C12N9/1205 , C12N15/8241
摘要: This invention relates to an isolated nucleic acid fragment encoding a protein kinase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the protein kinase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the protein kinase in a transformed host cell.
摘要翻译: 本发明涉及编码蛋白激酶的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或部分蛋白激酶的嵌合基因,其中嵌合基因的表达导致转化宿主细胞中蛋白激酶水平的改变。
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公开(公告)号:US6165792A
公开(公告)日:2000-12-26
申请号:US370253
申请日:1999-08-09
IPC分类号: C07K14/415 , C12N15/29 , C12N15/82 , C12N15/63 , C12N15/74
CPC分类号: C12N15/8254 , C07K14/415 , C12N15/8251
摘要: This invention relates to an isolated nucleic acid fragment encoding a amino acid transporter. The invention also relates to the construction of a chimeric gene encoding all or a portion of the amino acid transporter, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the amino acid transporter in a transformed host cell.
摘要翻译: 本发明涉及编码氨基酸转运蛋白的分离的核酸片段。 本发明还涉及在正义或反义方向上编码全部或部分氨基酸转运蛋白的嵌合基因的构建,其中嵌合基因的表达导致转化的宿主细胞中氨基酸转运蛋白水平的改变 。
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公开(公告)号:US08993840B2
公开(公告)日:2015-03-31
申请号:US12917024
申请日:2010-11-01
CPC分类号: C12N15/8247 , C07K14/415 , C12N9/1029 , C12Q1/6895 , C12Q2600/13 , C12Q2600/16 , C12Y203/0102
摘要: This invention relates to polynucleotide sequences encoding SUT2 or SUT4 sucrose transporter genes. Methods for increasing seed oil content and evaluating increased oil content in a plant seed are described. The compositions and methods disclosed herein employ a variety of sequences that encode sucrose transporters and a variety of sequences that influence fatty acid accumulation, including for example, DGAT, Lec1 and ODP1 transcription factor. In specific embodiments, overexpression of SUT2 and/or SUT4 sucrose transporters in combination with DGAT genes further increase plant seed oil production compared to a high oil plant comprising recombinant DNA constructs that do not overexpress SUT2 or SUT4 transporters.
摘要翻译: 本发明涉及编码SUT2或SUT4蔗糖转运蛋白基因的多核苷酸序列。 描述了增加种子油含量和评估植物种子中增加的油含量的方法。 本文公开的组合物和方法使用编码蔗糖转运蛋白的多种序列和影响脂肪酸积累的多种序列,包括例如DGAT,Lec1和ODP1转录因子。 在具体实施方案中,与包含不过表达SUT2或SUT4转运蛋白的重组DNA构建体的高油植物相比,SUT2和/或SUT4蔗糖转运蛋白与DGAT基因的过表达进一步增加植物种子油产量。
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