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    CONFIGURATION OF INITIAL CONTROL PARAMETERS IN PHOTODETECTORS FOR MULTI-COLOR FLOW CYTOMETRY
    82.
    发明申请
    CONFIGURATION OF INITIAL CONTROL PARAMETERS IN PHOTODETECTORS FOR MULTI-COLOR FLOW CYTOMETRY 审中-公开
    用于多色流动细胞计数的光电子体中的初始控制参数的配置

    公开(公告)号：US20100256943A1

    公开(公告)日：2010-10-07

    申请号：US12752387

    申请日：2010-04-01

    申请人： Albert David Donnenberg ,  Vera Svobodova Donnenberg

    发明人： Albert David Donnenberg ,  Vera Svobodova Donnenberg

    IPC分类号： G12B13/00 ,  G06F19/00

    CPC分类号： G01N15/1012 ,  G01J3/10 ,  G01J3/36 ,  G01N15/1425 ,  G01N2015/1477

    摘要： System(s) and method(s) for initial configuration of photodectors in a multi-color flow cytometer to mitigate spectral compensation. Configuration is iterative and automated, and optimizes calibration of control parameters or amplification control parameters subject to constraints that ensure satisfactory, e.g., optimal or within predetermined threshold(s), spectral compensation. Iterative configuration can include at least a first loop for constrained minimization of spectral overlap for a set of operational condition, and a second loop for configured settings acceptance.

    摘要翻译： 用于多色流式细胞仪中的光电探测器初始配置的系统和方法，以减轻光谱补偿。 配置是迭代和自动的，并且优化控制参数或放大控制参数的校准，受限于确保令人满意的，例如最佳或在预定阈值内的频谱补偿。 迭代配置可以包括用于一组操作条件的频谱重叠的约束最小化的第一循环和用于配置的设置接受的第二循环。

    DROPLET FORMATION APPARATUS AND METHODS FOR FORMING DROPLET AND CALIBRATING PARTICLE SIZE MEASUREMENT APPARATUS
    85.
    发明申请
    DROPLET FORMATION APPARATUS AND METHODS FOR FORMING DROPLET AND CALIBRATING PARTICLE SIZE MEASUREMENT APPARATUS 审中-公开
    成型装置及其制备方法和校准颗粒尺寸测量装置

    公开(公告)号：US20090033934A1

    公开(公告)日：2009-02-05

    申请号：US12166747

    申请日：2008-07-02

    申请人： Hidetoshi Katou ,  Makoto Yamaguchi ,  Yoshinori Yamashita

    发明人： Hidetoshi Katou ,  Makoto Yamaguchi ,  Yoshinori Yamashita

    IPC分类号： G01N15/02

    CPC分类号： G01N15/1012 ,  B41J2/1433 ,  B41J2/175 ,  B41J2/17556 ,  B41J2/19

    摘要： A droplet formation apparatus is provided for calibrating a particle size measurement apparatus. A vessel stores sample liquid. A pressure unit applies predetermined pressure to the sample liquid in the vessel. An oscillator is provided to one surface of the vessel for applying oscillation, which has a predetermined frequency, to the sample liquid in the vessel. An orifice is provided to an other surface of the vessel. The orifice has at least one discharge hole. The orifice is configured to form a droplet, which has a predetermined particle size, from the sample liquid and configured to discharge the droplet in accordance with the predetermined pressure and the predetermined frequency applied to the sample liquid in the vessel.

    摘要翻译： 提供了用于校准粒度测量装置的液滴形成装置。 船舶储存样品液体。 压力单元对容器中的样品液体施加预定的压力。 振荡器设置在容器的一个表面上，用于向容器中的样品液体施加具有预定频率的振荡。 孔口设置在容器的另一个表面上。 孔口具有至少一个排出孔。 孔口构造成从样品液体形成具有预定粒度的液滴，并且构造成根据预定压力和施加到容器中的样品液体的预定频率排出液滴。

    Methods for controlling one or more parameters of a flow cytometer type measurement system
    87.
    发明授权
    Methods for controlling one or more parameters of a flow cytometer type measurement system 有权
    用于控制流式细胞仪型测量系统的一个或多个参数的方法

    公开(公告)号：US07318336B2

    公开(公告)日：2008-01-15

    申请号：US10918647

    申请日：2004-08-13

    申请人： Wayne D. Roth ,  Douglas E. Moore

    发明人： Wayne D. Roth ,  Douglas E. Moore

    IPC分类号： G01N21/00

    CPC分类号： G01N15/1404 ,  G01N15/1012 ,  G01N21/645 ,  G01N2015/1415 ,  G01N2015/1477 ,  G01N2201/12746

    摘要： Various methods for controlling one or more parameters of a flow cytometer type measurement system are provided. One embodiment includes monitoring parameter(s) of the measurement system during measurements of sample microspheres. The method also includes altering the parameter(s) in real time based on the monitoring. Another method includes monitoring a temperature proximate to the measurement system. One such method includes altering a bias voltage of an avalanche photo diode in response to the temperature using empirically derived data. A different such method includes altering output signals of a photomultiplier tube in response to the temperature using a characteristic curve. Some methods include monitoring a temperature of a fluid, in which sample microspheres are disposed, that will flow through the flow cytometer type measurement system. This method also includes determining a velocity of the sample microspheres in the measurement system from a viscosity of the fluid at the temperature.

    摘要翻译： 提供了用于控制流式细胞仪型测量系统的一个或多个参数的各种方法。 一个实施例包括在样品微球的测量期间监测测量系统的参数。 该方法还包括基于监视实时更改参数。 另一种方法包括监测靠近测量系统的温度。 一种这样的方法包括使用经验导出的数据来响应于温度来改变雪崩光电二极管的偏置电压。 不同的这种方法包括使用特性曲线响应于温度改变光电倍增管的输出信号。 一些方法包括监测将流过流式细胞仪类型测量系统的流体样品微球的设置温度。 该方法还包括从该温度下的流体的粘度确定测量系统中的样品微球的速度。

    Wide range constant concentration particle generating system
    88.
    发明申请
    Wide range constant concentration particle generating system 失效
    宽范围恒定浓度粒子发生系统

    公开(公告)号：US20070028662A1

    公开(公告)日：2007-02-08

    申请号：US11193642

    申请日：2005-07-29

    申请人： Qiang Wei ,  Ichiro Asano

    发明人： Qiang Wei ,  Ichiro Asano

    IPC分类号： G01N15/00

    CPC分类号： G01N15/1012 ,  G01N1/2211 ,  G01N15/0266 ,  G01N15/065 ,  G01N2001/2223 ,  G01N2001/2264

    摘要： A particle generating system includes an aerosol generator, an ejector diluter, and an aerosol diluter. The ejector diluter receives the generated aerosol and dilutes the aerosol to an expected raw concentration. The aerosol diluter further dilutes the aerosol to a concentration in the range of 0% to 100% of the expected raw concentration. The aerosol diluter includes a mini cyclone for diluting the aerosol. The particle generating system may be configured to provide variable concentrations of monodisperse or polydisperse aerosols for instrument calibration. The system may provide constant concentrations in the range of 0% to 100% of the raw concentration. The mini cyclone makes the system compact, and the system may be portable.

    摘要翻译： 颗粒产生系统包括气溶胶发生器，喷射稀释器和气溶胶稀释器。 喷射器稀释器接收产生的气溶胶并将气溶胶稀释至预期的原始浓度。 气溶胶稀释剂进一步将气溶胶稀释至浓度在预期原料浓度的0％至100％的范围内。 气溶胶稀释器包括用于稀释气溶胶的微型旋风分离器。 颗粒产生系统可以被配置为提供可变浓度的单分散或多分散气溶胶用于仪器校准。 该系统可以提供在原始浓度的0％至100％范围内的恒定浓度。 微型旋风使系统紧凑，系统可以便携。

    Determination of sperm concentration and viability for artificial insemination
    89.
    发明授权
    Determination of sperm concentration and viability for artificial insemination 有权
    人工授精精子浓度和生存力的测定

    公开(公告)号：US07070917B1

    公开(公告)日：2006-07-04

    申请号：US09914765

    申请日：2000-03-06

    申请人： Preben Christensen ,  Jens Peter Stenvang

    发明人： Preben Christensen ,  Jens Peter Stenvang

    IPC分类号： A01N1/02

    CPC分类号： G01N15/14 ,  G01N15/1012 ,  G01N2015/149

    摘要： A method for the simultaneous determination of the total concentration of sperm cells and the proportion of live sperm cells in a semen sample is provided. By selective staining of live, dead and/or dying sperm cells, for example using one or more fluorochromes, the detection means can distinguish between live, dead and/or dying sperm cells, for example by detection of the fluorescent response from the sperm cells. The selective staining may be performed at room temperature and in concentrations below standard concentrations. The determination may be performed by selective counting of cells of each fluorescent quality for example by using a flow cytometer having internal concentration standard means. Furthermore, the likelihood of fertilizing a female animal with an insemination dose, taken from a semen sample having a known total concentration of sperm cells and a known proportion of live sperm cells, is predicted on the basis of the thus determined total concentration of sperm cells and the proportion of live sperm cells.

    摘要翻译： 提供了同时测定精液细胞总浓度和活精子细胞在精液样品中的比例的方法。 通过例如使用一种或多种荧光染料选择性染色活的，死亡的和/或死亡的精子细胞，检测手段可以区分活的，死亡的和/或死亡的精子细胞，例如通过检测来自精子细胞的荧光反应 。 选择性染色可以在室温和低于标准浓度的浓度下进行。 可以通过例如通过使用具有内部浓度标准装置的流式细胞仪对每种荧光质量的细胞进行选择性计数来进行测定。 此外，基于由此确定的精子细胞总浓度预测从具有已知的精子细胞总浓度的精液样品和已知比例的活精子细胞获得授精剂量的雌性动物施用的可能性 和活精子细胞的比例。

    Procedure for controlling the enumeration of the absolute count of cells or other particles present in a sample
    90.
    发明授权
    Procedure for controlling the enumeration of the absolute count of cells or other particles present in a sample 失效
    用于控制样品中存在的细胞或其他颗粒的绝对计数的计数的程序

    公开(公告)号：US06913901B2

    公开(公告)日：2005-07-05

    申请号：US10078558

    申请日：2002-02-14

    申请人： José Alberto Orfao de Matos Correia e Vale

    发明人： José Alberto Orfao de Matos Correia e Vale

    IPC分类号： G01N15/10 ,  G01N15/14 ,  C12Q1/02 ,  C12P35/06

    CPC分类号： G01N15/1012 ,  G01N2015/1406

    摘要： Procedure for controlling the enumeration of the absolute count of cells (or other particles) present in a sample. The procedure consists of the following: to prepare, in known quantities and proportions, a mixture or stock solution of two or more populations of reference particles of differing characteristics; to add a known quantity of this mixture of reference particles to a known volume of the sample which contains the cells (or other particles) to be counted; to measure, in a flow cytometer, the sample containing both the cells (or other particles), the events of which are to be counted, and the mixture of different populations of reference particles; to calculate the absolute number of cells (or other particles) present in the sample to be counted and; to check that the proportion between the different reference particles present in the sample measured concurs with the proportion that exists between them in the initial mixture or stock solution which contained the different populations of reference particles prior to adding it to the sample.

    摘要翻译： 用于控制样品中存在的细胞（或其他颗粒）的绝对计数的计数的程序。 该方法包括以下步骤：以已知的量和比例制备具有不同特征的两个或更多个参比颗粒群的混合物或储备溶液; 将已知量的参考颗粒混合物添加到包含要计数的细胞（或其他颗粒）的样品的已知体积中; 在流式细胞仪中测量含有细胞（或其他颗粒），其事件将被计数的样品和不同参考颗粒群体的混合物; 计算待计数样品中存在的细胞（或其他颗粒）的绝对数; 以检查测量样品中存在的不同参考颗粒之间的比例与添加到样品中之前含有参考颗粒群的初始混合物或储备溶液中之间存在的比例是否一致。