公开(公告)号：US20030185801A1

公开(公告)日：2003-10-02

申请号：US10002750

申请日：2001-11-15

发明人： Ronald Vogels ,  Menzo Jans Emco Havenga ,  Majid Mehtali

IPC分类号： A61K048/00 ,  C12N007/00 ,  C12N015/861 ,  C12N005/06 ,  A01N063/00 ,  C12N007/01 ,  C12N005/00 ,  C12N005/02 ,  C12N015/86

CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N7/025 ,  C12N15/861 ,  C12N2710/10041 ,  C12N2710/10051 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/00 ,  C12N2840/20

摘要： A packaging cell line capable of complementing recombinant adenoviruses based on serotypes from subgroup B, preferably adenovirus type 35. The cell line is preferably derived from primary, diploid human cells (e.g., primary human retinoblasts, primary human embryonic kidney cells and primary human amniocytes) which are transformed by adenovirus E1 sequences either operatively linked on one DNA molecule or located on two separate DNA molecules, the sequences being operatively linked to regulatory sequences enabling transcription and translation of encoded proteins. Also disclosed is a cell line derived from PER.C6 (ECACC deposit number 96022940), which cell expresses functional Ad35 E1B sequences. The Ad35-E1B sequences are driven by the E1B promoter or a heterologous promoter and terminated by a heterologous poly-adenylation signal. The new cell lines are useful for producing recombinant adenoviruses designed for gene therapy and vaccination. The cell lines can also be used for producing human recombinant therapeutic proteins such as human growth factors and human antibodies. In addition, the cell lines are useful for producing human viruses other than adenovirus such as influenza virus, herpes simplex virus, rotavirus, measles virus.

摘要翻译： 能够基于来自亚组B，优选腺病毒35的血清型补充重组腺病毒的包装细胞系。细胞系优选衍生自原代，二倍体人细胞（例如，原代人成视网膜细胞，原代人胚胎肾细胞和原代人羊膜细胞） 其通过可操作地连接在一个DNA分子上或位于两个分离的DNA分子上的腺病毒E1序列转化，所述序列可操作地连接到能够转录和翻译编码的蛋白质的调控序列。 还公开了衍生自PER.C6（ECACC保藏号96022940）的细胞系，该细胞表达功能性Ad35 E1B序列。 Ad35-E1B序列由E1B启动子或异源启动子驱动，并通过异源多聚腺苷酸化信号终止。 新的细胞系可用于产生用于基因治疗和疫苗接种的重组腺病毒。 细胞系也可以用于生产人重组治疗蛋白质，例如人生长因子和人抗体。 此外，细胞系可用于生产除腺病毒如流感病毒，单纯疱疹病毒，轮状病毒，麻疹病毒之外的人类病毒。

公开(公告)号：US20030170211A1

公开(公告)日：2003-09-11

申请号：US10357095

申请日：2003-02-03

发明人： Jaap Goudsmit ,  Jerald C. Sadoff ,  Wayne Koff

IPC分类号： A61K048/00 ,  C12N001/21 ,  A01N063/00 ,  C12N001/20 ,  C12N015/00 ,  C12N015/09 ,  C12N015/63 ,  C12N015/70 ,  C12N015/74

CPC分类号： C12N15/63 ,  A61K39/00 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/523 ,  A61K2039/5256 ,  C12N15/85 ,  C12N15/86 ,  C12N15/87 ,  C12N2740/16034 ,  C12N2760/14034 ,  C12N2770/36143 ,  Y02A50/476 ,  Y02A50/484

摘要： The present invention is directed to a bacterial delivery system for delivering alphavirus replicon DNA into an animal or animal cells with the replicon encoding one or more heterologous genes to be expressed in the animal or the animal cells. The bacteria are invasive bacteria or attenuated invasive bacteria engineered to contain a DNA vector that encodes the alphavirus replicon in a eukaryotic expression cassette. The heterologous gene can encode an antigen, a therapeutic agent, an immunoregulatory agent, an anti-sense RNA, a catalytic RNA, a protein, a peptide, an antibody or an antigen-binding fragment of an antibody. In a preferred embodiment, the heterologous gene encodes one or more antigens useful as a vaccine for HIV. In addition to the bacterial delivery system, the invention provides methods of introducing and expressing the heterologous gene(s) in animal or animal cells and methods of stimulating or inducing an immune response.

摘要翻译： 本发明涉及一种细菌递送系统，用于将编码在动物或动物细胞中表达的一种或多种异源基因的复制子递送至动物或动物细胞中的甲病毒复制子DNA。 细菌是侵入性细菌或减毒侵入性细菌，其被工程化以含有编码真核表达盒中的甲病毒复制子的DNA载体。 异源基因可编码抗体的抗原，治疗剂，免疫调节剂，反义RNA，催化RNA，蛋白质，肽，抗体或抗原结合片段。 在优选的实施方案中，异源基因编码可用作HIV疫苗的一种或多种抗原。 除了细菌递送系统之外，本发明提供在动物或动物细胞中引入和表达异源基因的方法以及刺激或诱导免疫应答的方法。

公开(公告)号：US20030152554A1

公开(公告)日：2003-08-14

申请号：US10324240

申请日：2002-12-19

发明人： Haim B. Gunner ,  Ming-Jung Coler ,  William A. Torello

IPC分类号： A01N063/00 ,  C12N001/20

CPC分类号： C12R1/07 ,  A01N63/00 ,  C12N11/14

摘要： A composition comprising bacteria and an inert carrier is disclosed. The carrier can be porous, ceramic particles. The composition can also include a growth medium. Bacteria in such a composition can comprise a novel strain designated APM-1. APM-1 is a Gram-positive, aerobic, motile rod, and appears to be most closely related to Bacillus spp. Also disclosed are methods of using the disclosed compositions for controlling plant fungal diseases.

摘要翻译： 公开了包含细菌和惰性载体的组合物。 载体可以是多孔的陶瓷颗粒。 组合物还可以包括生长培养基。 这种组合物中的细菌可以包含称为APM-1的新菌株。 APM-1是一种革兰氏阳性，需氧，活动杆，似乎与芽孢杆菌最相关。 还公开了使用所公开的组合物来控制植物真菌疾病的方法。

公开(公告)号：US20030138410A1

公开(公告)日：2003-07-24

申请号：US09939537

申请日：2001-08-24

发明人： Brian Seed ,  Babak Banapour ,  Charles Romeo ,  Waldemar Kolanus

IPC分类号： A61K048/00 ,  C12Q001/70 ,  A01N063/00 ,  C12N005/06 ,  C12N005/16

CPC分类号： A61K35/15 ,  A61K35/13 ,  A61K35/17 ,  A61K35/545 ,  A61K38/00 ,  C07K14/705 ,  C07K14/70503 ,  C07K14/7051 ,  C07K14/70514 ,  C07K14/70535 ,  C07K19/00 ,  C07K2319/00 ,  C12N9/1205 ,  C12N2799/023

摘要： Disclosed is a method of directing a cellular immune response against an HIV-infected cell in a mammal involving administering to the mammal an effective amount of therapeutic cells which express a membrane-bound, proteinaceous chimeric receptor comprising (a) an extracellular portion which includes a fragment of CD4 which is capable of specifically recognizing and binding the HIV-infected cell but which does not mediate HIV infection and (b) an intracellular portion which is capable of signalling the therapeutic cell to destroy the receptor-bound HIV-infected cell. Also disclosed are cells which express the chimeric receptors and DNA and vectors encoding the chimeric receptors.

摘要翻译： 公开了一种在哺乳动物中引导针对HIV感染细胞的细胞免疫应答的方法，包括向哺乳动物施用有效量的表达膜结合蛋白质嵌合受体的治疗细胞，所述受体细胞包含（a）细胞外部分，其包括 能够特异性识别和结合HIV感染细胞但不介导HIV感染的CD4片段，和（b）能够发信号通知治疗细胞破坏受体结合的HIV感染细胞的细胞内部分。 还公开了表达嵌合受体的细胞和编码嵌合受体的DNA和载体。

公开(公告)号：US20030124096A1

公开(公告)日：2003-07-03

申请号：US10260451

申请日：2002-10-01

申请人： Western Health Care Network

发明人： Stephen A. Locarnini ,  Angeline I. Bartholomeusz ,  Thein T. Aye ,  Robert A. de Man

IPC分类号： C12Q001/70 ,  C07H021/04 ,  C12N009/22 ,  C12N007/00 ,  A01N063/00 ,  A01N065/00 ,  A61K048/00 ,  C12N007/01

CPC分类号： C12N7/00 ,  C07K14/005 ,  C12N9/1276 ,  C12N2730/10122

摘要： The present invention relates generally to viral variants exhibiting reduced sensitivity to particular agents and/or reduced interactivity with immunological reagents. More particularly the present invention is directed to hepatitis B variants exhibiting complete or partial resistance to nucleoside analogues and/or reduced interactivity with antibodies to viral surface components. The present invention further contemplates assays for detecting such viral variants which assays are useful in monitoring anti-viral therapeutic regimes.

摘要翻译： 本发明一般涉及对特定试剂表现出降低的敏感性和/或降低与免疫试剂的相互作用的病毒变体。 更具体地，本发明涉及对核苷类似物表现出完全或部分抗性和/或与病毒表面组分的抗体降低的相互作用的乙型肝炎变体。 本发明还考虑了用于检测这种病毒变体的测定法，该测定可用于监测抗病毒治疗方案。

公开(公告)号：US20030118558A1

公开(公告)日：2003-06-26

申请号：US10259197

申请日：2002-09-27

发明人： Janakiraman Ramachandran ,  Sriram Padmanabhan ,  Bharathi Sriram

IPC分类号： A61K045/00 ,  C12N007/00 ,  A01N063/00 ,  C12N007/01

CPC分类号： C12N7/00 ,  A61K35/76 ,  C12N2795/10111 ,  C12N2795/10132 ,  C12N2795/10161 ,  Y02A50/475

摘要： The present invention features therapeutic bacteriophage deficient in the lysin protein (nullLys minusnull phage). Lys minus bacteriophage are incapable of facilitating efficient lysis of the bacterial host since the enzymatic activity of the lysin of the phage is needed for breaking down the peptidoglycan layer of the bacterial cell wall. Lys minus bacteriophage retain activity in invasion of its appropriate bacterial host, destruction of the bacterial genome, and replication, which are sufficient to inhibit bacterial growth and replication. Therefore, the therapeutic Lys minus phage stops the spread of infection by the bacterial pathogen without lysis of the bacterium. This approach is attractive as it also prevents the release of the phage progeny, thus reducing or eliminating the potential for generation of immune responses against the phage. The incapacitated bacterial pathogen is then removed by the normal defense systems such as phagocytes and macrophages.

摘要翻译： 本发明的特征在于溶酶蛋白（“Lys减”噬菌体）中缺乏的治疗性噬菌体。 Lys minus噬菌体不能促进细菌宿主的有效溶解，因为需要噬菌体溶素的酶活性来分解细菌细胞壁的肽聚糖层。 Lys减去噬菌体在其适当的细菌宿主的侵入中保留活性，破坏细菌基因组和复制，其足以抑制细菌生长和复制。 因此，治疗性Lys-噬菌体阻止了细菌病原体感染的扩散，而不会破坏细菌。 这种方法是有吸引力的，因为它也可以防止噬菌体后代的释放，从而减少或消除针对噬菌体产生免疫应答的可能性。 然后通过正常的防御系统如吞噬细胞和巨噬细胞去除失能的细菌病原体。

公开(公告)号：US20030091550A1

公开(公告)日：2003-05-15

申请号：US10195542

申请日：2002-07-16

申请人： INSTITUT PASTEUR

发明人： Alejandra Bravo ,  Didier Lereclus ,  Herve Agaisse ,  Sylvie Salamitou ,  Vincent Sanchis

IPC分类号： A01N063/00 ,  C12N001/21

CPC分类号： C12R1/075 ,  C07K14/325 ,  C07K2319/00

摘要： The present invention relates particularly to a strain of Bacillus thuringiensis characterized in that it expresses the gene sigma E (nullE) and does not sporulate at all or little sporulates or does not produce any viable spores. The invention also relates to a pesticide composition containing said strain of Bacillus thuringiensis.

摘要翻译： 本发明特别涉及苏云金芽孢杆菌菌株，其特征在于其表达基因σE（sigmaE），并且根本不发生孢子形成或少量孢子形成或不产生任何可行的孢子。 本发明还涉及含有所述苏云金芽孢杆菌菌株的农药组合物。

公开(公告)号：US20030064487A1

公开(公告)日：2003-04-03

申请号：US10192805

申请日：2002-07-09

申请人： Ultra Biotech Limited

发明人： Lingyu Zhang

IPC分类号： C12N013/00 ,  A01N063/00 ,  A01N063/04

CPC分类号： C05F11/08 ,  C12N1/14 ,  C12N13/00

摘要： The present invention provides biological fertilizer compositions that comprise yeast cells that have an enhanced ability to fix atmospheric nitrogen, decompose phosphorus minerals and compounds, decompose potassium minerals and compounds, decompose complex carbon compounds, over produce growth factors, and over produce ATP. The biological fertilizer composition of the invention can replace mineral fertilizers in supplying nitrogen, phosphorus, and potassium to crop plants. Methods of manufacturing the biological fertilizer compositions and methods of uses are also encompassed.

公开(公告)号：US20030049241A1

公开(公告)日：2003-03-13

申请号：US10140283

申请日：2002-05-08

发明人： Gunnard K. Jacobson ,  Setsuko O. Jolly ,  Joseph J. Sedmak ,  Thomas J. Skatrud ,  John M. Wasileski

IPC分类号： A01N063/04 ,  A01N063/00 ,  C12P001/00

CPC分类号： C12R1/645 ,  A23K20/179 ,  A23L5/44 ,  C12N13/00 ,  C12P23/00

摘要： Phaffia rhodozyma strains are described which produce greater than 3,000 ppm astaxanthin based on dry yeast solids when cultivated in a volume of nutrient medium of at least about 1,500 liters and containing in excess of 4 percent, preferably in excess of 6 percent, dry yeast solids. These and other strains are cultivated by an improved fermentation method comprising extending the maturation phase of the fermentation by one or more various techniques including exposing the yeast cells to a low-intensity light, slow feeding the cells with a rapidly metabolized energy source, e.g. glucose, and replacing the rapidly metabolized energy source with a slowly metabolized energy source, e.g. gylcerol. The cells of these strains are incorporated into animal feeds, particularly feeds for salmonid fishes, to impart or enhance the red pigmentation of these animals and products made from these animals.

摘要翻译： 描述了红酵母酵母菌株，当在至少约1,500升的营养培养基的体积中培养并含有超过4％，优选超过6％的干燥酵母固体时，其基于干酵母固体产生大于3,000ppm的虾青素。 这些和其它菌株通过改进的发酵方法培养，包括通过一种或多种各种技术延长发酵的成熟期，包括将酵母细胞暴露于低强度光，用快速代谢的能量来缓慢喂养细胞，例如， 葡萄糖，并用缓慢代谢的能量源代替快速代谢的能量源，例如， gylcerol。 这些菌株的细胞被并入动物饲料，特别是鲑鱼的饲料中，以赋予或增强这些动物的红色色素沉着和由这些动物制成的产品。

公开(公告)号：US20030041349A1

公开(公告)日：2003-02-27

申请号：US09991262

申请日：2001-11-20

发明人： Peter Daniel Christian ,  Karl Hienrich Julius Gordon ,  Terry Nelson Hanzlik

IPC分类号： A01H005/00 ,  C12N007/00 ,  A01N063/00

CPC分类号： C12N15/8286 ,  A01N63/00 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/40 ,  C07K2319/55 ,  C07K2319/61 ,  C07K2319/735 ,  C12N7/00 ,  C12N15/62 ,  C12N15/82 ,  C12N2710/14143 ,  C12N2770/00021 ,  C12N2770/00022 ,  C12N2770/00023 ,  Y02A40/162

摘要： The present invention relates to an isolated small RNA virus capable of infecting insect species including Heliothis species, and to the nucleotide sequences and proteins encoded thereby. The invention contemplates uses of the virus in controlling insect attack in plants.

摘要翻译： 本发明涉及能够感染包括Heliothis种类的昆虫物种的分离的小RNA病毒，以及由此编码的核苷酸序列和蛋白质。 本发明考虑了病毒在植物中防治昆虫攻击的用途。