公开(公告)号：US6001628A

公开(公告)日：1999-12-14

申请号：US596257

申请日：1996-04-18

申请人： Jens Kossmann ,  Michael Emmermann ,  Ivar Virgin

发明人： Jens Kossmann ,  Michael Emmermann ,  Ivar Virgin

IPC分类号： C12N15/09 ,  C12N5/10 ,  C12N9/44 ,  C12N15/56 ,  C12N15/82 ,  C12P19/04 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12N15/8245 ,  C12N9/2451

摘要： DNA sequences are described which on the codogenic strand code plant debranching enzymes, whose transcripts formed in transgenic plants code new proteins with the enzymatic activity of debranching enzymes which in transgenic plants reduce the degree of branching of amylopectin starch and DNA sequences which on the codogenic strand code plant debranching enzymes, whose transcripts formed in transgenic plants prevent the synthesis of proteins with the enzymatic activity of debranching enzymes, which in the transgenic plants increases the degree of branching of amylopectin starch, as well as plasmids on which these DNA sequences are localized, which can be introduced into plant cells and plants. Also described is a process for the production of plants changed by genetic engineering whose amylopectin starch is modified, and the modified starch obtainable from these plants.

摘要翻译： PCT No.PCT / EP94 / 02623 Sec。 371日期：1996年4月18日 102（e）1996年4月18日PCT PCT 1994年8月8日PCT公布。 出版物WO95 / 04826 日期1995年2月16日描述了在编码植物脱支酶的编码基因编码植物脱支酶上的DNA序列，其在转基因植物中形成的转录物用转基因植物的酶活性代​​谢新的蛋白质，其在转基因植物中降低支链淀粉和DNA序列的支化度 其在代码链编码植物脱支酶上，其在转基因植物中形成的转录物阻止蛋白质的合成，其具有脱支酶的酶活性，其在转基因植物中增加了支链淀粉淀粉的支化度，以及这些质粒 DNA序列是局部化的，其可以被引入植物细胞和植物中。 还描述了由支链淀粉改性的遗传工程改变的植物的生产方法，以及可从这些植物获得的改性淀粉。

公开(公告)号：US5985622A

公开(公告)日：1999-11-16

申请号：US785396

申请日：1997-01-21

申请人： Ralf Mattes ,  Kathrin Klein ,  Hubert Schiweck ,  Markwart Kunz ,  Mohammed Munir

发明人： Ralf Mattes ,  Kathrin Klein ,  Hubert Schiweck ,  Markwart Kunz ,  Mohammed Munir

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/24 ,  C12N9/44 ,  C12N9/90 ,  C12N15/56 ,  C12N15/61 ,  C12P19/16 ,  C12P19/24 ,  C12R1/19 ,  C12P19/12

CPC分类号： C12N9/2451 ,  C12N9/90 ,  C12P19/16 ,  C12P19/24

摘要： The invention relates to sucrose isomerases, to DNA sequences coding therefor, and to novel processes for the production of non-cariogenic sugars.

摘要翻译： 本发明涉及蔗糖异构酶，对其编码的DNA序列以及用于生产非致龋糖的新方法。

公开(公告)号：US5912413A

公开(公告)日：1999-06-15

申请号：US410784

申请日：1995-03-24

申请人： Alan M. Myers ,  Martha Graham James

发明人： Alan M. Myers ,  Martha Graham James

IPC分类号： C12N9/44 ,  C12N15/29 ,  C12N15/82 ,  A01H4/00 ,  A01H5/00

CPC分类号： C12N9/2457 ,  C12N15/8245 ,  C12N9/2451 ,  C12N9/246 ,  C12Y302/01041 ,  C12Y302/01068

摘要： SU1, a starch debranching enzyme active in maize endosperm (Zea mays), and the cDNA and gene sequences encoding SU1 are disclosed. The amino acid sequence is significantly similar to that of bacterial isoamylases, enzymes that hydrolyze .alpha.-(1.fwdarw.6) glycosidic bonds. Amino acid sequence similarity establishes SU1 as a member of the .alpha.-amylase superfamily of starch hydrolytic enzymes. Also disclosed are antibodies reactive with the SU1 protein, methods of producing antibodies to the SU1 protein, methods of producing fusion proteins including SU1 and methods of producing transgenic plants with a modified su1 gene. The expressed SU1 protein can serve as a replacement for the bacterial and fungal enzymes currently used in the starch processing industry.

摘要翻译： SU1是在玉米胚乳（Zea mays）中活性的淀粉脱支酶，并且公开了编码SU1的cDNA和基因序列。 氨基酸序列与细菌异戊酰酶（水解α-（1-> 6）糖苷键的酶显着相似。 氨基酸序列相似性建立了SU1作为淀粉水解酶的α-淀粉酶超家族的成员。 还公开了与SU1蛋白反应的抗体，产生SU1蛋白的抗体的方法，产生包括SU1的融合蛋白的方法和用修饰的su1基因产生转基因植物的方法。 表达的SU1蛋白可以用作淀粉加工业中目前使用的细菌和真菌酶的替代物。

公开(公告)号：US5882906A

公开(公告)日：1999-03-16

申请号：US765123

申请日：1996-09-09

申请人： Carsten Sj.o slashed.holm ,  Garabed Antranikian

发明人： Carsten Sj.o slashed.holm ,  Garabed Antranikian

IPC分类号： C12N9/28 ,  C12N9/44 ,  C12P7/06 ,  C12P19/14 ,  C12P19/16 ,  C12N1/12 ,  C13K1/06

CPC分类号： C12N9/2451 ,  C12N9/2417 ,  C12P19/14 ,  C12P19/16 ,  C12P7/06 ,  Y02E50/17

摘要： The present invention relates to an amylase isolated from Staphalothermus marinus, DSM 3639, having a temperature optima of 95.degree.-105.degree. C., determined at pH 5.5 and a pH optima of 4.5 to 5.5, determined at 95.degree. C. The amylase can be employed in the production of sweeteners and ethanol.

摘要翻译： PCT No.PCT / DK95 / 00096 Sec。 371日期1996年9月9日 102（e）日期1996年9月9日PCT 1995年3月2日PCT公布。 WO95 / 23851 PCT出版物 日期1995年9月8日本发明涉及一种从Staphalothermus marinus（DSM 3639）分离的淀粉酶，其温度最高为95〜-105℃，在pH5.5下测定，pH最佳值为4.5〜5.5，在95℃下测定 C.淀粉酶可用于生产甜味剂和乙醇。

公开(公告)号：US5811277A

公开(公告)日：1998-09-22

申请号：US391769

申请日：1995-02-21

申请人： Tsuei Yun Fang ,  Long Liu Lin ,  Wen Hwei Hsu

发明人： Tsuei Yun Fang ,  Long Liu Lin ,  Wen Hwei Hsu

IPC分类号： C12N9/44 ,  C12N9/24

CPC分类号： C12N9/246 ,  C12Y302/01068

摘要： The present invention relates to a method for recovery and purification of isoamylase by raw starch adsorption method. Various raw starches are used to recover the isoamylase produced by a bacterium such as Pseudomonas amyloderamosa. The method comprises using raw starch to adsorb isoamylase under conditions suitable for such adsorption and isolating the adsorbed enzyme. The isoamylase adsorption rate varied with the species of raw starch. Additionally, raw starch can be recycled.

摘要翻译： 本发明涉及通过生淀粉吸附法回收和纯化异淀粉酶的方法。 使用各种原料淀粉来回收由细菌如淀粉假单胞菌（Pseudomonas amyloderamosa）产生的异淀粉酶。 该方法包括使用生淀粉在适于吸附和分离吸附的酶的条件下吸附异淀粉酶。 异淀粉酶的吸附速率随生淀粉的种类而变化。 另外，可以回收生淀粉。

公开(公告)号：US5773277A

公开(公告)日：1998-06-30

申请号：US445206

申请日：1995-05-18

申请人： Nobukazu Hashimoto ,  Hideo Mochizuki

发明人： Nobukazu Hashimoto ,  Hideo Mochizuki

IPC分类号： C12N9/88 ,  C12N9/44

CPC分类号： C12N9/88

摘要： A crystallizable, purified chondroitinase ABC having a molecular weight of about 100,000 dalton by the measurement of the SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and the measurement by the gel permeation chromatography method, having alanine as the N-terminal amino acid and proline as the C-terminal amino acid is disclosed. A process for the purification of the crystallizable purified chondroitinase ABC comprising removing nucleic acid from an surfactant solution extract obtained from cells of chondroitinase ABC-producing microorganisms and chromatographically treating by concentration gradient elution using a weak cation exchange resin or a strong cation exchange resin is disclosed. A composition comprising a chondroitinase and serum albumin, gelatin, or a nonionic surfactant is disclosed. The enzyme is isolated from Proteus vulgaris ATCC 6896.

摘要翻译： 通过SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）的测量和通过凝胶渗透色谱法测定的具有约100,000道尔顿分子量的可结晶纯化的软骨素酶ABC，其具有丙氨酸作为N-末端氨基酸和脯氨酸 因为公开了C-末端氨基酸。 公开了一种纯化可结晶纯化软骨素酶ABC的方法，包括从由软骨素酶ABC产生的微生物的细胞获得的表面活性剂溶液提取物中除去核酸，并使用弱阳离子交换树脂或强阳离子交换树脂进行色谱法处理。 。 公开了包含软骨素酶和血清白蛋白，明胶或非离子表面活性剂的组合物。 该酶从普通变形杆菌ATCC 6896中分离。

公开(公告)号：US5750876A

公开(公告)日：1998-05-12

申请号：US476519

申请日：1995-06-07

申请人： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  Bradley Martin Krohn

发明人： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  Bradley Martin Krohn

IPC分类号： A01H5/00 ,  C08B30/00 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/12 ,  C12N9/28 ,  C12N9/44 ,  C12N15/09 ,  C12N15/54 ,  C12N15/55 ,  C12N15/82 ,  C12P19/04 ,  C12R1/125 ,  C12R1/19 ,  C12R1/91 ,  C12N15/31

CPC分类号： C12N9/246 ,  C12N15/8245 ,  C12N9/1241 ,  C12N9/2451 ,  C12Y302/01068

摘要： A method of producing plant products containing modified starch content, including higher ratios of amylose to amylopectin, increase in intermediate material, or amylopectin having fewer branches or altered branching pattern. Also provided are DNA constructs and transformed plant cells useful in that method. The preferred method uses isoamylase from a Flavobacterium sp., more preferably in combination with a gene encoding ADPglucose pyrophosphorylase. Also disclosed are the gene from Flavobacterium sp. and transformed bacterial and plant cells containing a derivative thereof.

摘要翻译： 一种生产含有改性淀粉含量的植物产品的方法，其包括较高比例的直链淀粉与支链淀粉，中间物质的增加或具有较少枝条或改变的分枝花样的支链淀粉。 还提供了在该方法中有用的DNA构建体和转化的植物细胞。 优选的方法使用来自黄杆菌属的异淀粉酶，更优选与编码ADP葡萄糖焦磷酸化酶的基因组合。 还公开了来自黄杆菌属（Flavobacterium sp。）的基因。 并转化含有其衍生物的细菌和植物细胞。

公开(公告)号：US5457037A

公开(公告)日：1995-10-10

申请号：US1797

申请日：1993-01-08

申请人： Angelo Tognoni ,  Paolo Carrera ,  Barbara Camerini ,  Giuliano Galli ,  Giuseppe Lucchese ,  Guido Grandi ,  Carlo Di Gennaro

发明人： Angelo Tognoni ,  Paolo Carrera ,  Barbara Camerini ,  Giuliano Galli ,  Giuseppe Lucchese ,  Guido Grandi ,  Carlo Di Gennaro

IPC分类号： C12N1/20 ,  C12N1/21 ,  C12N9/44 ,  C12N15/09 ,  C12N15/55 ,  C12N15/56 ,  C12P21/02 ,  C12R1/09 ,  C12R1/19 ,  C12R1/38 ,  C12N15/31 ,  C12N15/75

CPC分类号： C12Y302/01068 ,  C12N9/246

摘要： The structural gene encoding isoamylase from Pseudomonas SMP 1 is obtained. Also described are vectors comprising the isoamylase gene and host microorganisms transformed by these vectors. The transformed host microorganisms are capable of expressing and/or secreting mature isoamylase having biological activity.

摘要翻译： 获得编码来自假单胞菌SMP1的异淀粉酶的结构基因。 还描述了包含异淀粉酶基因和由这些载体转化的宿主微生物的载体。 转化的宿主微生物能够表达和/或分泌具有生物活性的成熟异淀粉酶。

公开(公告)号：US5147796A

公开(公告)日：1992-09-15

申请号：US825314

申请日：1992-01-27

申请人： Katsutoshi Ara ,  Katsuhisa Saeki ,  Kazuaki Igarashi ,  Susumu Ito

发明人： Katsutoshi Ara ,  Katsuhisa Saeki ,  Kazuaki Igarashi ,  Susumu Ito

IPC分类号： C12N9/44

CPC分类号： C12N9/2417 ,  C12N9/2457 ,  C12Y302/01041 ,  Y10S435/832

摘要： An isolated alkaline pullulanase Y having .alpha.-amylase activity; a microorganism producing the alkaline pullulanase Y; and a process for producing the alkaline pullulanase Y are disclosed. The alkaline pullulanase Y having .alpha.-amylase activity has its optimum pH at higher alkaline range than conventional alkaline pullulanases and exhibits excellent stability in a wide pH range. Further, the alkaline pullulanase Y has strong resistance to almost all detergent components such as surfactants, chelating agents, proteases for detergents, and the like. Thus the alkaline pullulanase Y can advantageously be used as a detergent component.

摘要翻译： 具有α-淀粉酶活性的分离的碱性支链淀粉酶Y; 产生碱性支链淀粉酶Y的微生物; 并公开了制备碱性支链淀粉酶Y的方法。 具有α-淀粉酶活性的碱性支链淀粉酶Y具有比常规碱性支链淀粉酶更高的碱性范围的最佳pH，并且在宽pH范围内表现出优异的稳定性。 此外，碱性支链淀粉酶Y对几乎所有的洗涤剂组分如表面活性剂，螯合剂，用于洗涤剂的蛋白酶等都具有很强的抗性。 因此，碱性支链淀粉酶Y可以有利地用作洗涤剂组分。

公开(公告)号：US5147795A

公开(公告)日：1992-09-15

申请号：US575434

申请日：1990-08-30

申请人： Katsutoshi Ara ,  Kazuaki Igarashi ,  Katsuhisa Saeki ,  Susumu Ito

发明人： Katsutoshi Ara ,  Kazuaki Igarashi ,  Katsuhisa Saeki ,  Susumu Ito

IPC分类号： C12N9/44

CPC分类号： C12Y302/01041 ,  C12N9/2457 ,  Y10S435/832

摘要： A novel alkaline pullulanase; a microorganism producing the alkaline pullulanase; and a process for producing the alkaline pullulanase are disclosed. The alkaline pullulanase of the present invention has a higher optimum pH range (pH 9.5-11) than conventional alkaline pullulanases and shows excellent stability in a wider pH range. It has also an optimum temperature of higher than 50.degree. C. and is thermally stable up to 40.degree. C. The alkaline pullulanase has also strong reistance to almost all detergent components such as surfactants, chelating agents, proteases for detergents, and the like. The alkaline pullulanase can advantageously be used as a detergent component. Also, it can be utilized for producing many kinds of oligosaccharides and also together with .alpha.-amylase for producing various monosaccharides.