公开(公告)号：US20240318216A1

公开(公告)日：2024-09-26

申请号：US18733787

申请日：2024-06-04

申请人： Chr. Hansen HMO GmbH

发明人： Stefan Jennewein ,  Dirk Wartenberg

IPC分类号： C12P19/18 ,  C12N9/10 ,  C12N15/70

CPC分类号： C12P19/18 ,  C12N9/1051 ,  C12N15/70 ,  C12Y204/01146

摘要： The present invention relates to methods for the production of oligosaccharides in genetically modified bacterial host cells, as well as to the genetically modified host cells used in the methods. The genetically modified host cell comprises at least one recombinant glycosyltransferase, and at least one nucleic acid sequence coding for a protein enabling the export of the oligosaccharide.

公开(公告)号：US12037622B2

公开(公告)日：2024-07-16

申请号：US17323737

申请日：2021-05-18

申请人： Chr. Hansen HMO GmbH

发明人： Stefan Jennewein ,  Dirk Wartenberg

IPC分类号： C12P19/18 ,  C12N1/20 ,  C12N9/10 ,  C12N15/70

CPC分类号： C12P19/18 ,  C12N9/1051 ,  C12N15/70 ,  C12Y204/01146

摘要： The present invention relates to methods for the production of oligosaccharides in genetically modified bacterial host cells, as well as to the genetically modified host cells used in the methods. The genetically modified host cell comprises at least one recombinant glycosyltransferase, and at least one nucleic acid sequence coding for a protein enabling the export of the oligosaccharide.

公开(公告)号：US11981947B2

公开(公告)日：2024-05-14

申请号：US17814333

申请日：2022-07-22

申请人： Chr. Hansen HMO GmbH

发明人： Stefan Jennewein

IPC分类号： C12P19/12 ,  C07H1/00 ,  C07H1/06 ,  C12N1/20 ,  C12N15/72 ,  C12P19/00 ,  C12P19/14 ,  C12P19/26 ,  C12R1/19

CPC分类号： C12P19/26 ,  C07H1/00 ,  C07H1/06 ,  C12N1/205 ,  C12N15/72 ,  C12P19/00 ,  C12P19/12 ,  C12P19/14 ,  C12Y302/01 ,  C12Y302/01023 ,  C12N2800/101 ,  C12R2001/19

摘要： The present invention relates to the use of one or more glycosidases in the process for the production and/or purification of a produced desired oligosaccharide. The process is preferably a microbial fermentation process using a host microorganism, which may also comprise nucleic acids expressing sugar catabolic pathway proteins suitable for the degradation of saccharides otherwise hindering the purification of the desired oligosaccharide.

公开(公告)号：US20240110213A1

公开(公告)日：2024-04-04

申请号：US18267958

申请日：2021-12-22

申请人： Chr. Hansen HMO GmbH

发明人： Henning Frerigmann ,  Christian Trötschel ,  Dirk Wartenberg ,  Stefan Jennewein

IPC分类号： C12P19/04 ,  C07K14/245 ,  C12N15/52

CPC分类号： C12P19/04 ,  C07K14/245 ,  C12N15/52

摘要： Disclosed are genetically engineered microbial cells for the production of an oligosaccharide of interest, wherein said microbial cells possess a variant of the E. coli lactose permease LacY which exhibits a reduced transport activity for lactose, and methods for producing an oligosaccharide by using said genetically engineered microbial cells.

公开(公告)号：US20230399669A1

公开(公告)日：2023-12-14

申请号：US18207453

申请日：2023-06-08

申请人： Chr. Hansen HMO GmbH

发明人： Stefan Jennewein ,  Dirk Wartenberg ,  Katja Parschat

IPC分类号： C12P19/12 ,  C12N1/20 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/90 ,  C12P19/04 ,  C12P19/18

CPC分类号： C12P19/12 ,  C12N1/20 ,  C12N9/1051 ,  C12N9/1205 ,  C12N9/16 ,  C12N9/90 ,  C12P19/04 ,  C12P19/18 ,  C12N2500/34 ,  C12Y204/01038 ,  C12Y207/01004 ,  C12Y207/01011 ,  C12Y207/01056 ,  C12Y207/07009 ,  C12Y301/03011 ,  C12Y501/03002 ,  C12Y504/02002

摘要： Disclosed are genetically engineered microbial cells for the production of oligosaccharides comprising a galactose-β1,4-glucose moiety at their reducing end, wherein said microbial cells are able to produce said oligosaccharides in the absence of exogenously added lactose, and a method of producing said oligosaccharides using said microbial cells.

公开(公告)号：US20240199673A1

公开(公告)日：2024-06-20

申请号：US18417034

申请日：2024-01-19

申请人： Chr. Hansen HMO GmbH

发明人： Stefan JENNEWEIN ,  Markus HELFRICH ,  Benedikt ENGELS

IPC分类号： C07H1/06 ,  A23L33/00 ,  A23L33/125 ,  A23L33/135 ,  C07H3/06

CPC分类号： C07H1/06 ,  A23L33/125 ,  A23L33/135 ,  A23L33/30 ,  C07H3/06

摘要： Disclosed is a method for purifying sialylated oligosaccharides from a fermentation broth, cell-lysate or biocatalytic reaction mixture for obtaining high amounts of desired sialylated oligosaccharides in high purity. The method is particular suitable for the large-scale economic purification of sialylated human milk oligosaccharides (such as 3′-sialyllactose, 6′-sialyllactose or sialylated lacto-N-tetraose derivatives) from microbial fermentation, using recombinant bacterial cells or yeast cells. The obtained material is of high purity and can be used for food or medical application such like medical nutrition products, infant formula, dietary supplements, general nutrition products (e.g. dairy drinks).

公开(公告)号：US20230304052A1

公开(公告)日：2023-09-28

申请号：US18013047

申请日：2021-06-11

申请人： Chr. Hansen HMO GmbH

发明人： Markus Englert ,  Stefan Jennewein ,  Henning Frerigmann ,  Katja Parschat ,  Christian Trötschel

IPC分类号： C12P19/04 ,  C12N15/70 ,  C12N15/52 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90

CPC分类号： C12P19/04 ,  C12N15/70 ,  C12N15/52 ,  C12N9/0006 ,  C12Y101/01271 ,  C12N9/1051 ,  C12Y204/01065 ,  C12N9/1205 ,  C12N9/1241 ,  C12Y207/07013 ,  C12N9/88 ,  C12Y402/01048 ,  C12N9/90 ,  C12Y504/02008 ,  C12N2800/101

摘要： Disclosed are genetically-engineered gram-negative bacterial cells for the production of an oligosaccharide of interest, wherein the bacterial cell possesses a saccharide transporter and/or a porin being expressed from a recombinant gene or a deregulated endogenous gene, thereby facilitating the translocation of the oligosaccharide of interest from the cell's cytoplasm into the cell's environment. Also disclosed are methods for producing an oligosaccharide of interest which used said genetically-engineered gram-negative bacterial cells.

公开(公告)号：US11732282B2

公开(公告)日：2023-08-22

申请号：US16765833

申请日：2018-11-15

申请人： Chr. Hansen HMO GmbH

发明人： Markus Helfrich ,  Stefan Jennewein

IPC分类号： C12P19/02 ,  A23L33/125 ,  A23L33/00 ,  A61K31/7012 ,  C12P19/26 ,  C07H1/08 ,  C07H3/08 ,  C07H1/06 ,  C07H7/027

CPC分类号： C12P19/02 ,  A23L33/125 ,  A23L33/40 ,  A61K31/7012 ,  C07H1/06 ,  C07H1/08 ,  C07H3/08 ,  C07H7/027 ,  C12P19/26

摘要： The present invention concerns an efficient way to isolate L-fucose from a fermentation broth. The L-fucose contained in the fermentation broth is produced by microbial fermentation (bacterial or yeasts). The inventive process comprises a step of removing biomass from the fermentation broth, a step of subjecting the resulting solution to at least one of a cationic ion exchanger treatment and an anionic ion exchanger treatment and a step of removing salts after the ion exchanger treatment. The process can provide L-fucose in powder form, in granulated form as well as in form of L-fucose crystals.

公开(公告)号：US20220403433A1

公开(公告)日：2022-12-22

申请号：US17814337

申请日：2022-07-22

申请人： Chr. Hansen HMO GmbH

发明人： Stefan Jennewein

IPC分类号： C12P19/26 ,  C12P19/14 ,  C07H1/00 ,  C07H1/06 ,  C12P19/12

摘要： The present invention relates to the use of one or more glycosidases in the process for the production and/or purification of a produced desired oligosaccharide. The process is preferably a microbial fermentation process using a host microorganism, which may also comprise nucleic acids expressing sugar catabolic pathway proteins suitable for the degradation of saccharides otherwise hindering the purification of the desired oligosaccharide.

公开(公告)号：US20220145342A1

公开(公告)日：2022-05-12

申请号：US17434967

申请日：2020-02-28

申请人： Chr Hansen HMO GmbH

发明人： Stefan JENNEWEIN ,  Dirk WARTENBERG

IPC分类号： C12P19/02 ,  C12P19/04 ,  C12P19/18

摘要： Disclosed are genetically engineered microbial cells for the production of a carbohydrate of interest, wherein the microbial cells possess an increased intracellular availability of at least one sugar phosphate, and produce the carbohydrate of interest when being cultivated in a culture medium containing a mixed monosaccharide feedstock as main carbon and energy source. Also disclosed is a fermentative method of producing a carbohydrate of interest by cultivating said genetically engineered microbial cell in the presence of a mixed monosaccharide feedstock as main carbon and energy source.