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    Methods for genetic analysis of DNA to detect sequence variances
    9.
    发明授权
    Methods for genetic analysis of DNA to detect sequence variances 失效
    遗传分析DNA检测序列差异的方法

    公开(公告)号：US07659054B1

    公开(公告)日：2010-02-09

    申请号：US09697028

    申请日：2000-10-25

    申请人： Jeffrey Olson ,  Vincent P. Stanton, Jr.

    发明人： Jeffrey Olson ,  Vincent P. Stanton, Jr.

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/686 ,  C12Q1/6858 ,  C12Q2525/313

    摘要： The presently claimed invention concerns a method for biasing the amplification of the DNA molecules in a sample such that a nucleic acid molecule having a specific nucleotide at a selected position (e.g., a polymorphic site) is preferentially amplified relative to an otherwise identical nucleic acid molecule not having the specific nucleotide at the selected position. The method entails amplification of target nucleic acid molecules using a pair of primers one of which causes the incorporation into the amplification product of a sequence that interferes with amplification of nucleic acid molecules not having the specific nucleotide at the selected position. The method is useful for preferentially amplifying at least a portion of one allele of a gene relative to another, different allele of the gene in a sample containing both alleles of the gene.

    摘要翻译： 本发明涉及一种用于偏置样品中DNA分子的扩增的方法，使得在选定位置（例如，多态性位点）具有特定核苷酸的核酸分子相对于其它相同的核酸分子优先扩增 在选定位置没有特异性核苷酸。 该方法需要使用一对引物扩增靶核酸分子，其中一个引物引入掺入在选定位置不具有特定核苷酸的核酸分子的扩增的序列的扩增产物。 该方法可用于相对于含有该基因的两个等位基因的样品中该基因的另一不同等位基因优先扩增一个等位基因的至少一部分。