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公开(公告)号：US08021875B2
公开(公告)日：2011-09-20
申请号：US10229786
申请日：2002-08-27
CPC分类号： C12N15/85 , C07K14/765 , C12N2830/30 , C12N2830/34 , C12N2830/42 , C12N2830/50 , C12N2830/85
摘要： The present invention relates to compositions and methods for increasing long term expression in vitro and in vivo, comprising sequences and gene expression cassettes that increase expression of genes to which they are operably linked.
摘要翻译： 本发明涉及用于在体外和体内增加长期表达的组合物和方法，其包含增加其可操作连接的基因表达的序列和基因表达盒。
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公开(公告)号：US20110207799A1
公开(公告)日：2011-08-25
申请号：US13032029
申请日：2011-02-22
申请人： David B. Rozema , David L. Lewis , Darren H. Wakefield , Torsten Hoffmann , Erik Kitas , Peter Mohr , Philipp Hadwiger , Wilma Thuer , Linda Valis
发明人： David B. Rozema , David L. Lewis , Darren H. Wakefield , Torsten Hoffmann , Erik Kitas , Peter Mohr , Philipp Hadwiger , Wilma Thuer , Linda Valis
IPC分类号： A61K31/7105 , A61K31/711 , A61K31/713 , A61P1/16 , A61P35/00 , A61P31/12 , A61P3/00 , A61P3/10 , A61P3/06
CPC分类号： C12N15/113 , A61K9/08 , A61K31/713 , A61K47/32 , A61K47/549 , A61K47/60 , A61K47/65 , A61K48/0041 , C12N15/111 , C12N15/87 , C12N2310/14 , C12N2310/141 , C12N2310/3515 , C12N2320/32
摘要： The present invention is directed compositions for targeted delivery of RNA interference (RNAi) polynucleotides to hepatocytes in vivo. Targeted RNAi polynucleotides are administered together with co-targeted delivery polymers. Delivery polymers provide membrane penetration function for movement of the RNAi polynucleotides from outside the cell to inside the cell. Reversible modification provides physiological responsiveness to the delivery polymers.
摘要翻译： 本发明涉及用于将体内RNA干扰（RNAi）多核苷酸靶向递送至肝细胞的组合物。 靶向RNAi多核苷酸与共靶向递送聚合物一起施用。 递送聚合物提供膜穿透功能，用于将RNAi多核苷酸从细胞外部移动到细胞内。 可逆修饰提供对递送聚合物的生理反应性。
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公开(公告)号：US07816337B2
公开(公告)日：2010-10-19
申请号：US10780484
申请日：2004-02-17
CPC分类号： C12N15/111 , A61K47/58 , A61K48/00 , A61K48/0041 , C12N15/87 , C12N2310/351 , C12N2320/32
摘要： Described is a process for delivering a biologically active compound to a cell by reversibly linking the compound to a membrane active polymer. In particular, polymer-polynucleotide conjugates are described. Methods for reversibly modifying the polymers to decrease cellular toxicity and improve efficacy are provided.
摘要翻译： 描述了通过可逆地将化合物与膜活性聚合物连接而将生物活性化合物递送至细胞的方法。 特别地，描述了聚合物 - 多核苷酸缀合物。 提供了可逆修饰聚合物以降低细胞毒性并提高功效的方法。
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公开(公告)号：US07524680B2
公开(公告)日：2009-04-28
申请号：US10095681
申请日：2002-03-11
IPC分类号： C12N15/88
CPC分类号： C12N15/88 , A61K47/58 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041 , C12N15/87
摘要： An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
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5.发明申请Reversible Hydrophobic Modification of Drugs for Improved Delivery to Cells 审中-公开用于改善细胞递送的药物的可逆疏水改性公开(公告)号：US20090074885A1
公开(公告)日：2009-03-19
申请号：US12260679
申请日：2008-10-29
IPC分类号： A61K31/704 , A61K33/24 , A61K31/337 , A61P35/04 , A61K31/195
CPC分类号： A61K31/58 , A61K9/0019 , A61K31/195 , A61K31/337 , A61K31/695 , A61K31/704 , A61K47/54 , A61K47/542 , C07F7/10 , C07F7/1804 , C07J1/00
摘要： Described are drug formulations that increase regional delivery of the drugs to cells. Methods for reversibly increasing the hydrophobicity of a drug through hydrolytically labile attachment of a hydrophobic moiety and methods for delivering the modified drug to cells are described. Hydrophobic modification increases drug delivery, while lability minimizes entry of the drug into non-target cells.
摘要翻译： 描述了增加药物对细胞的区域递送的药物制剂。 描述了通过疏水部分的水解不稳定连接可逆地增加药物的疏水性的方法和将修饰的药物递送至细胞的方法。 疏水性修饰增加药物递送，同时使药物最小化进入非靶细胞。
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公开(公告)号：US07501394B2
公开(公告)日：2009-03-10
申请号：US11337121
申请日：2006-01-20
申请人： Alexander V. Sokoloff , So Wong , Jon A. Wolff , Sean D. Monahan , James Ludtke , Lori Higgs , Darren H. Wakefield , Magdolna G. Sebestyén
发明人： Alexander V. Sokoloff , So Wong , Jon A. Wolff , Sean D. Monahan , James Ludtke , Lori Higgs , Darren H. Wakefield , Magdolna G. Sebestyén
IPC分类号： A61K38/00
CPC分类号： C07K14/005 , A61K41/00 , C12N2795/10222
摘要： We describe compounds that bind to and are internalized by hepatocytes. Association of these compounds to other molecules or complexes can be used to target the molecules or complexes to hepatocytes in vivo or in vitro.
摘要翻译： 我们描述与肝细胞结合并被内化的化合物。 这些化合物与其他分子或复合物的结合可用于在体内或体外靶向肝细胞的分子或复合物。
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公开(公告)号：US07498017B2
公开(公告)日：2009-03-03
申请号：US11154494
申请日：2005-06-16
CPC分类号： A61K48/0041 , C12N15/87
摘要： Cyclic amidinium containing compounds and their methods of preparation are described. Compositions containing these compounds facilitate delivery of biologically active polymers to cells in vitro and in vivo.
摘要翻译： 描述了含有环脒的化合物及其制备方法。 含有这些化合物的组合物有助于将体内和体内的生物活性聚合物递送至细胞。
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公开(公告)号：US07473419B2
公开(公告)日：2009-01-06
申请号：US11268276
申请日：2005-11-07
CPC分类号： A61K48/0041 , A61K38/00 , A61K48/00 , A61K48/0008 , A61K48/0075 , A61K48/0083 , C12N15/87
摘要： Disclosed is a process for providing for expression of an exogenous nucleic acid in an extravascular parenchymal cell of a mammal. The nucleic acid is inserted into a vessel of a mammal and the permeability of the vessel is increased. Increasing permeability of the vessel allows delivery of the nucleic acid to an extravascular parenchymal cell.
摘要翻译： 公开了一种提供外源核酸在哺乳动物血管外实质细胞中表达的方法。 将核酸插入哺乳动物的容器中并增加血管的渗透性。 增加容器的渗透性允许将核酸递送至血管外实质细胞。
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公开(公告)号：US08017109B2
公开(公告)日：2011-09-13
申请号：US12140703
申请日：2008-06-17
申请人： Darren H. Wakefield , David B. Rozema , Sean D. Monahan , Lori Higgs , Jon A. Wolff , James E. Hagstrom
发明人： Darren H. Wakefield , David B. Rozema , Sean D. Monahan , Lori Higgs , Jon A. Wolff , James E. Hagstrom
IPC分类号： A61K31/74
CPC分类号： C12N15/87 , A01K2217/058 , A01K2267/0362 , A61K47/58
摘要： The present invention is directed to membrane active polymers useful for cellular delivery of compounds. Described are polyconjugates systems that incorporate targeting, anti-opsonization, anti-aggregation, and transfection activities into small biocompatible in vivo delivery conjugates. The use of reversible modification provides for physiologically responsive activity modulation.
摘要翻译： 本发明涉及可用于细胞递送化合物的膜活性聚合物。 描述了将靶向，抗调理作用，抗聚集和转染活性结合到小的生物相容性体内递送缀合物中的多偶联体系。 使用可逆修饰提供了生理反应活性调节。
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公开(公告)号：US07871818B2
公开(公告)日：2011-01-18
申请号：US10765668
申请日：2004-01-27
申请人： David B. Rozema , Darren Wakefield
发明人： David B. Rozema , Darren Wakefield
CPC分类号： A61K47/58
摘要： We describe a class of polymaleic anhydride polymers capable of disrupting cell membranes. Co-delivery of these polymers with biologically active compounds increases cellular cytoplasmic delivery of the compounds.
摘要翻译： 我们描述了一类能够破坏细胞膜的聚马来酸酐聚合物。 这些聚合物与生物活性化合物的共同递送增加化合物的细胞胞质递送。
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