PHOTOERADICATION OF MICROORGANISMS WITH PULSED PURPLE OR BLUE LIGHT

    公开(公告)号:US20220193445A1

    公开(公告)日:2022-06-23

    申请号:US17687904

    申请日:2022-03-07

    摘要: The present invention is directed to a system and method for photoeradication of microorganisms from a target. The method includes the step of obtaining test data for a plurality of experiments each of which comprises irradiating test microorganisms with a plurality of light pulses having a wavelength that ranges from 380 nm to 500 nm. The light pulses have a plurality of pulse parameters (peak irradiance, pulse duration, and off time between adjacent light pulses) and are provided at a radiant exposure that ranges from 0.5 J/cm2 to 60 J/cm2 during each of a plurality of irradiation sessions. The test data comprises a survival rate for the test microorganisms after irradiation with the light pulses. The method also includes the step of analyzing the test data to identify the pulse parameters for the light pulses and the radiant exposure for each of the irradiation sessions that result in a desired survival rate for the test microorganisms. The method further includes the step of irradiating the microorganisms of the target with light pulses having the identified pulse parameters at the identified radiant exposure for each of the irradiation sessions so as to photoeradicate all or a portion of the microorganisms.

    COMPOUNDS FOR FLUORESCENCE SENSING OF DUPLEX FORMATION

    公开(公告)号:US20200262862A1

    公开(公告)日:2020-08-20

    申请号:US16346708

    申请日:2017-11-09

    摘要: Herein reported are new tricyclic cytidine compounds, such as 8-diethylamino-tC (8-DEA-tC), that respond to DNA and/or RNA duplex formation with up to a 20-fold increase in fluorescent quantum yield as compared with the free nucleoside, depending on neighboring bases. This turn-on response to duplex formation is by far the greatest of any reported nucleoside analogue that can participate in Watson-Crick base pairing. Measurements of the quantum yield of 8-DEA-tC mispaired with adenosine and, separately, opposite an abasic site show that there is almost no fluorescence increase without the formation of correct Watson-Crick hydrogen bonds. Kinetic isotope effects from the use of deuterated buffer show that the duplex protects 8-DEA-tC against quenching by excited state proton transfer. DFT calculations provide a rationale for the observed photophysical properties that is dependent on duplex integrity and the electronic structure of the analogue.

    Hybrid Metal and Carbon or Glassy Carbon MEMS u-ECOG Electrode and Microelectrode Structures
    10.
    发明申请
    Hybrid Metal and Carbon or Glassy Carbon MEMS u-ECOG Electrode and Microelectrode Structures 审中-公开
    混合金属和碳或玻璃碳MEMS u-ECOG电极和微电极结构

    公开(公告)号:US20160073920A1

    公开(公告)日:2016-03-17

    申请号:US14855738

    申请日:2015-09-16

    摘要: Microelectromechanical system are disclosed that include at least one electrode, microelectrode or combination thereof, wherein the at least one electrode comprises a carbon material, a glassy carbon material or a combination thereof. Contemplated systems are suitable for μ-ECoG arrays. Additional microelectromechanical systems are disclosed that include at least one electrode, microelectrode or combination thereof, wherein the at least one electrode comprises a carbon material, a glassy carbon material or a combination thereof; at least one substrate, surface, layer or a combination thereof, wherein the at least one electrode, microelectrode or combination thereof is disposed on, coupled with or otherwise layered on the at least one substrate, surface, layer or a combination thereof; and at least one bump pad, wherein the at least one electrode, microelectrode or combination thereof is coupled with the at least one bump pad via at least one conductive metal. A method of making a microelectromechanical system includes patterning a polymer precursor, a carbon-containing material or a combination thereof onto a surface, a substrate, at least one layer or a combination thereof; and heating or pyrolysing the polymer precursor, a carbon-containing material or a combination thereof in order to form a glassy carbon material. Uses of microelectromechanical systems are also contemplated to measure at least one electrical property in a mammal or for electrocorticography.

    摘要翻译: 公开了包括至少一个电极,微电极或其组合的微机电系统,其中所述至少一个电极包括碳材料,玻璃碳材料或其组合。 考虑的系统适用于μ-ECoG阵列。 公开了包括至少一个电极,微电极或其组合的附加微机电系统,其中所述至少一个电极包括碳材料,玻璃碳材料或其组合; 至少一个衬底,表面,层或其组合,其中所述至少一个电极,微电极或其组合设置在所述至少一个衬底,表面,层或其组合上,耦合或以其他方式分层; 和至少一个凸块焊盘,其中所述至少一个电极,微电极或其组合经由至少一个导电金属与所述至少一个凸块焊盘耦合。 一种制造微电子机械系统的方法包括将聚合物前体,含碳材料或其组合图案化到表面,基底,至少一层或其组合上; 并加热或热解聚合物前体,含碳材料或其组合以形成玻璃碳材料。 还考虑使用微机电系统来测量哺乳动物中的至少一种电性质或电皮质。