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34 条记录 (耗时 0.019 秒)

    Solid support having ligand immobilized thereon by using photocleavable linker
    1.
    发明授权
    Solid support having ligand immobilized thereon by using photocleavable linker 有权
    通过使用可光致发光的接头，其上固定有配体的固体支持物

    公开(公告)号：US07456022B2

    公开(公告)日：2008-11-25

    申请号：US11572972

    申请日：2005-07-28

    申请人： Masayuki Haramura ,  Akito Tanaka

    发明人： Masayuki Haramura ,  Akito Tanaka

    IPC分类号： B01J20/26 ,  G01N33/547

    CPC分类号： B01J20/289 ,  B01J20/261 ,  B01J20/286 ,  B01J20/321 ,  B01J20/3219 ,  B01J20/3251 ,  B01J20/3253 ,  B01J20/3255 ,  B01J2220/54 ,  G01N33/54353 ,  Y10T436/207497

    摘要： The present invention provides a method of analyzing the interaction between a ligand and a target molecule, which uses a solid support wherein the ligand is immobilized via a linker cleavable by photoirradiation, particularly a method of searching and purifying a target molecule. By intervening a linker specifically cleavable by photoirradiation between the ligand molecule and the solid support, release and elution of the target molecule from the solid support without using a ligand or salt at a high concentration can be enabled, and suppression of release and elution of a nonspecific protein can be enabled.

    摘要翻译： 本发明提供了一种分析配体和靶分子之间的相互作用的方法，其使用固体支持物，其中通过光照射可切割的接头固定配体，特别是搜索和纯化靶分子的方法。 通过插入通过配体分子和固体支持体之间的光照射可以特异性地切割的接头，可以使得目标分子从固体支持物中释放和洗脱而不使用高浓度的配体或盐，并且抑制释放和洗脱 可以启用非特异性蛋白质。

    Affinity Carrier and Method for Producing Thereof
    4.
    发明申请
    Affinity Carrier and Method for Producing Thereof 审中-公开
    亲和载体及其生产方法

    公开(公告)号：US20090298954A1

    公开(公告)日：2009-12-03

    申请号：US12308151

    申请日：2007-06-08

    申请人： Ken Hosoya ,  Tomoko Mori ,  Akito Tanaka ,  Teruki Takahashi

    发明人： Ken Hosoya ,  Tomoko Mori ,  Akito Tanaka ,  Teruki Takahashi

    IPC分类号： A61K47/32

    CPC分类号： B01J20/26 ,  B01J20/264 ,  B01J20/265 ,  B01J20/267 ,  C08F220/26 ,  C08F220/36 ,  C08F222/1006

    摘要： An affinity carrier not of a particle aggregation type but in a gel state having a bicontinuous structure composed of pores having an average diameter of micrometer size arranged continuously in a three-dimensional network structure and a skeletal phase abundant in organic matters, comprisingat least one compound having at least two functionalities as a crosslinking agent of a vinyl monomer compound, a methacrylate compound or an acrylate compound; anda copolymer of at least one monofunctional hydrophilic monomer as a ligand-carrying monomer and at least one monofunctional thinning monomer as a thinning agent for arbitrarily controlling a hydrophilic/hydrophobic environment in which a ligand carried by said ligand-carrying monomer is put,wherein a volume ratio of said crosslinking agent to “said copolymer of the monofunctional hydrophilic monomer and the mono functional thinning monomer” in said affinity carrier is 100 to 10:0 to 90.

    摘要翻译： 不具有粒子聚集型但凝胶状态的亲和载体，其具有由三维网状结构中连续排列的平均微米尺寸直径的孔和有机物丰富的骨架相构成的双连续结构，所述亲和载体包含至少一个 具有至少两种官能团的化合物作为乙烯基单体化合物，甲基丙烯酸酯化合物或丙烯酸酯化合物的交联剂; 和至少一种单官能亲水单体作为配体携带单体和至少一种单功能稀释单体的共聚物，作为用于任意控制所述配体携带单体携带的配位体的亲水/疏水环境的稀释剂，其中 所述亲和载体中所述交联剂与单功能亲水单体和单官能疏水单体的所述共聚物的体积比为100〜10：0〜90。

    Carboxylic acid compound having cyclopropane ring
    6.
    发明授权
    Carboxylic acid compound having cyclopropane ring 有权
    具有环丙烷环的羧酸化合物

    公开(公告)号：US07468389B2

    公开(公告)日：2008-12-23

    申请号：US10450816

    申请日：2001-12-18

    申请人： Tomoyuki Nishizaki ,  Akito Tanaka

    发明人： Tomoyuki Nishizaki ,  Akito Tanaka

    IPC分类号： A61K31/215 ,  C07C61/00 ,  C07C61/04

    CPC分类号： C07C61/04 ,  C07C53/134 ,  C07C57/26 ,  C07C61/12 ,  C07C61/28 ,  C07C61/35

    摘要： A carboxylic acid compound having cyclopropane ring(s) of the formula (I): wherein R is alkyl or alkenyl optionally having one or more 1,2-cyclopropylene in a carbon chain and/or optionally having cyclopropyl at the end of a chain,X is a single bond or alkylene, wherein the total number of carbon less the number of cyclopropane ring is 10-25, and a pharmaceutically acceptable salt thereof are provided. The compound (I) shows an LTP-like potentiation of synaptic transmission, allows slow metabolism in the living body, show a stable LTP-like potentiation of synaptic transmission, and is useful as an agent for LTP-like potentiation of synaptic transmission, a cognition-enhancing drug or an agent for the prophylaxis and treatment of dementia, a learning and memory disorder and a neurotransmitter release disorder.

    摘要翻译： 具有式（I）的环丙烷环的羧酸化合物：其中R是在碳链中任选具有一个或多个1,2-亚丙基的烷基或链烯基和/或在链末端任选具有环丙基的羧酸化合物， X是单键或亚烷基，其中提供环丙烷环数少于10-25的碳总数及其药学上可接受的盐。 化合物（I）显示突触传递的LTP样增强，允许活体中的代谢缓慢，表现出稳定的LTP样增强突触传递，并且可用作LTP样增强突触传递的药剂，a 认知增强药物或预防和治疗痴呆，学习和记忆障碍和神经递质释放障碍的药剂。

    Affinity resin
    8.
    发明申请
    Affinity resin 有权
    亲和树脂

    公开(公告)号：US20070167594A1

    公开(公告)日：2007-07-19

    申请号：US10573165

    申请日：2004-10-15

    申请人： Akito Tanaka ,  Takaaki Shiyama ,  Akira Yamazaki

    发明人： Akito Tanaka ,  Takaaki Shiyama ,  Akira Yamazaki

    IPC分类号： C08F26/00 ,  C08F126/00 ,  C08F226/00 ,  C07C69/73

    CPC分类号： G01N33/53 ,  A61K6/083 ,  B01D15/3804 ,  B01J20/267 ,  B01J20/289 ,  C08F220/36 ,  C08L33/10 ,  G01N33/543 ,  G01N33/545

    摘要： A resin which is prepared by polymerizing a monomer component incorporating a hydrophilic spacer, and a ligand-immobilized solid phase carrier obtained by immobilizing a ligand to the resin, are capable of reducing the non-specific adsorption of substances, other than the target molecule for the ligand, which mingle in the sample, to the resin and/or the ligand. Therefore, target molecule search, identification and the like with less noise are enabled.

    摘要翻译： 通过聚合包含亲水间隔基的单体组分和通过将配体固定在树脂上获得的配体固定的固相载体制备的树脂能够降低除了靶分子以外的物质的非特异性吸附 在样品中混合的配体与树脂和/或配体结合。 因此，能够实现具有较少噪声的靶分子搜索，识别等。

    Method of calibrating ligand specificity
    9.
    发明申请
    Method of calibrating ligand specificity 失效
    校准配体特异性的方法

    公开(公告)号：US20070122913A1

    公开(公告)日：2007-05-31

    申请号：US10573167

    申请日：2004-10-15

    申请人： Akito Tanaka ,  Akira Yamazaki

    发明人： Akito Tanaka ,  Akira Yamazaki

    IPC分类号： G01N33/558

    CPC分类号： G01N33/54313 ,  G01N33/54393 ,  Y10S435/973 ,  Y10T436/10 ,  Y10T436/25375

    摘要： In a method comprising comparing a solid phase carrier extract 1 obtained by pre-treating a sample with a ligand-immobilized solid phase carrier and a solid phase carrier extract 2 obtained by treating the pretreated sample again with a ligand-immobilized solid phase carrier in terms of the proteins contained therein, and identifying a protein whose content is remarkably decreased in the extract 2 compared to the extract 1, 1) the problem of the solubility of subject ligand, 2) the problem of the non-specific protein-denaturing effect of the subject ligand added, and the like, which have been problematic in antagonism experiments in target search using an affinity resin, have been solved.

    摘要翻译： 在包括将通过预处理样品获得的固相载体提取物1与固定配体的固相载体和固相载体提取物2进行比较的方法中，所述固相载体提取物2通过用配体固定的固相载体再次处理预处理的样品而得到 的蛋白质，并且鉴定与提取物1相比，提取物2中的含量显着降低的蛋白质，1）主体配体的溶解度的问题，2）非特异性蛋白质变性效应的问题 已经解决了使用亲和性树脂在靶搜索中的拮抗实验中存在问题的添加的受试者配体等。

    Reduced FK228 and use thereof
    10.
    发明授权
    Reduced FK228 and use thereof 有权
    减少FK228及其使用

    公开(公告)号：US07056884B2

    公开(公告)日：2006-06-06

    申请号：US10333063

    申请日：2001-07-09

    申请人： Hidenori Nakajima ,  Akito Tanaka ,  Minoru Yoshida ,  Sueharu Horinouchi

    发明人： Hidenori Nakajima ,  Akito Tanaka ,  Minoru Yoshida ,  Sueharu Horinouchi

    IPC分类号： A61K38/00 ,  C07K16/00

    CPC分类号： C07K11/02 ,  A61K38/00 ,  C07K5/101 ,  Y02P20/55

    摘要： The present invention relates to reduced FK228 of the formula (I) wherein R1 and R2 are the same or different and each is a hydrogen atom or a thiol-protecting group, or a salt thereof, and a histone deacetylase inhibitor containing this compound, an expression potentiator and a reactivation promoter of a transgene, and pharmaceutical agents containing them as active ingredients. The reduced FK228 or a salt thereof has a strong histone deacetylase inhibitory activity and this compound can be used for the prophylaxis or treatment of various diseases, in which histone deacetylation is involved, and for the gene therapy of such diseases.

    摘要翻译： 本发明涉及式（I）的还原型FK228，其中R 1和R 2相同或不同，各自为氢原子或硫醇保护基 或其盐，以及含有该化合物的组蛋白脱乙酰酶抑制剂，转基因的表达增强子和再活化启动子以及含有它们作为有效成分的药剂。 还原的FK228或其盐具有强的组蛋白脱乙酰酶抑制活性，该化合物可用于预防或治疗涉及组蛋白脱乙酰化的各种疾病和用于这些疾病的基因治疗。