公开(公告)号：US11860169B2

公开(公告)日：2024-01-02

申请号：US17015006

申请日：2020-09-08

Applicant: Chu Sainte-Justine

Inventor： Alain Moreau ,  Marie-Yvonne Akoume Ndong

IPC: G01N33/68 ,  G01N33/487 ,  G01N33/53 ,  A61F5/02 ,  A61K31/4045 ,  A61K33/04 ,  A61K45/06 ,  A61K31/713 ,  A61K38/19 ,  C12N15/113 ,  A61K38/08

CPC classification number: G01N33/6893 ,  A61F5/02 ,  A61F5/026 ,  A61K31/4045 ,  A61K31/713 ,  A61K33/04 ,  A61K38/19 ,  A61K45/06 ,  C12N15/1138 ,  G01N33/48728 ,  A61K38/08 ,  C12N2310/14 ,  C12N2320/30 ,  G01N33/53 ,  G01N2333/04 ,  G01N2333/726 ,  G01N2800/10 ,  G01N2800/50 ,  G01N2800/52 ,  A61K31/4045 ,  A61K2300/00 ,  A61K31/713 ,  A61K2300/00 ,  A61K33/04 ,  A61K2300/00

Abstract: The present invention provides a method of treating a subject in need thereof comprising classifying the subject into functional group FG1, FG2 or FG3, wherein i) when the subject is classified into the FG1 functional group, (A) the level of OPN or the activity of OPN in said subject is increased; (B) the subject is not treated with a brace; or (C) a combination of (A) and (B); and ii) when the subject is classified into the FG2 or FG3 functional group, (A) the level of OPN or the activity of OPN in said subject is decreased; (B) the subject is treated with a brace; or (C) a combination of (A) and (B).

公开(公告)号：US20190178896A1

公开(公告)日：2019-06-13

申请号：US16277565

申请日：2019-02-15

Applicant: Chu Sainte-Justine

Inventor： Alain Moreau ,  Marie - Yvonne Akoume Ndong

IPC: G01N33/68 ,  A61K38/19 ,  A61K45/06 ,  A61K31/713 ,  A61F5/02 ,  G01N33/487 ,  A61K33/04 ,  C12N15/113 ,  A61K31/4045

Abstract: A method of classifying a subject having idiopathic scoliosis (IS) or at risk of developing IS comprising: determining the cellular response to Gi stimulation in a cell sample from the subject in the presence of OPN; determining the cellular response to Gi stimulation in a cell sample from the subject in the absence of OPN; and comparing the cellular response obtained in the presence of OPN with the cellular response obtained in the absence of OPN, whereby the comparing step enables the classification of the subject into one IS functional group. Also provided is the use of the foregoing method to classify borderline subjects and kits for applying the methods.

公开(公告)号：US10073101B2

公开(公告)日：2018-09-11

申请号：US14807450

申请日：2015-07-23

Applicant: CHU Sainte-Justine

Inventor： Alain Moreau

IPC: G01N33/50 ,  G01N33/533 ,  G01N33/536 ,  G01N33/541 ,  G01N33/68 ,  A61K33/04 ,  A61F5/01 ,  A23L33/00

CPC classification number: G01N33/6893 ,  A23L33/30 ,  A23V2002/00 ,  A61F5/0102 ,  A61K33/04 ,  G01N33/6872 ,  G01N2800/108 ,  G01N2800/50

Abstract: A method for the prevention or treatment of scoliosis in a human subject comprising: (a)(i) measuring osteopontin (OPN) protein expression in a biological fluid sample from the subject over time; or (ii) measuring osteopontin (OPN) protein expression in a biological fluid sample from the subject and comparing the OPN protein expression to an OPN protein expression in a control biological fluid sample; (b) identifying the subject as being at risk of developing scoliosis when OPN protein expression increases in the subject sample over time; or when OPN protein expression is higher in the subject sample than that in the control sample; and (c) reducing OPN protein levels in the subject identified as being at risk of developing a scoliosis, thereby aiding in the prevention or treatment of scoliosis.

公开(公告)号：US20160291016A1

公开(公告)日：2016-10-06

申请号：US14898904

申请日：2014-06-17

Applicant: Chu Sainte-Justine

Inventor： Alain Moreau ,  Marie - Yvonne Akoume Ndong ,  Mohamed Elbakry

IPC: G01N33/573 ,  C12N15/113 ,  C12Q1/68

CPC classification number: G01N33/573 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2320/30 ,  C12Q1/42 ,  C12Q1/485 ,  C12Q1/6883 ,  C12Q2600/118 ,  C12Q2600/158 ,  C12Y207/01068 ,  C12Y301/03048 ,  G01N33/5047 ,  G01N33/5091 ,  G01N33/6893 ,  G01N2333/912 ,  G01N2333/91215 ,  G01N2333/916 ,  G01N2800/10 ,  G01N2800/50 ,  G01N2800/56

Abstract: Methods of stratifying a subject having or at risk for developing adolescent idiopathic scoliosis (AIS) into diagnostically or clinically useful subclasses are provided. The stratification is based on the subject's PTPμ expression and/or activity and/or PIPK1γ expression and/or activity. Also provided are methods of predicting the risk of developing a scoliosis also based on the subject's PTPμ expression and/or activity and/or PIPK1γ expression and/or activity; and methods of increasing GiPCR signaling in cells of a subject in need thereof comprising administering to the subject's cells an effective amount of an inhibitor of PIPK1γ tyrosine phosphorylation; an activator of PIPK1Y tyrosine dephosphorylation; and/or an inhibitor of PIPK1γ expression and/or activity.

Abstract translation: 提供了将患有发展青少年特发性脊柱侧弯（AIS）或具有发展青少年特发性脊柱侧凸（AIS）的危险性的受试者分为诊断或临床有用的亚类的方法。 分层是基于受试者的PTPμ表达和/或活性和/或PIPK1γ表达和/或活性。 还提供了基于受试者的PTPμ表达和/或活性和/或PIPK1γ表达和/或活性来预测发展脊柱侧凸的风险的方法; 以及在有需要的受试者的细胞中增加GiPCR信号传导的方法，包括向受试者的细胞施用有效量的PIPK1γ酪氨酸磷酸化抑制剂; PIPK1Y酪氨酸去磷酸化的激活剂; 和/或PIPK1γ表达和/或活性的抑制剂。

公开(公告)号：US20160258947A1

公开(公告)日：2016-09-08

申请号：US15159222

申请日：2016-05-19

Applicant: CHU SAINTE-JUSTINE

Inventor： ALAIN MOREAU

IPC: G01N33/566 ,  G01N33/74

CPC classification number: G01N33/566 ,  C12Q1/02 ,  C12Q1/527 ,  G01N33/56966 ,  G01N33/6893 ,  G01N33/74 ,  G01N2333/726 ,  G01N2800/10

Abstract: A composition for classifying a human pediatric subject having adolescent idiopathic scoliosis (AIS), comprising (a) a cell sample of the subject; and (b) a known melatonin-signaling pathway agonist, wherein said melatonin signaling pathway in said cell sample has been artificially induced by said melatonin-signaling pathway agonist, wherein the cell sample is selected from the group consisting of blood cell sample, osteoblast cell sample, osteoclast cell sample and myoblast cell sample, and whereby the composition enables the detection of the presence or absence of at least one impairment in the melatonin-signaling pathway in cells from said cell sample and thereby enables the classification of subject.

Abstract translation: 一种用于分类具有青少年特发性脊柱侧凸（AIS）的人类儿科受试者的组合物，其包含（a）所述受试者的细胞样品; 和（b）已知的褪黑激素信号通路激动剂，其中所述细胞样品中的所述褪黑激素信号通路已被所述褪黑激素信号通路激动剂人为诱导，其中所述细胞样品选自血细胞样品，成骨细胞 样品，破骨细胞样品和成肌细胞样品，并且由此组合物能够检测来自所述细胞样品的细胞中褪黑激素信号传导途径中至少一种损伤的存在或不存在，从而能够对受试者进行分类。

公开(公告)号：US20160223567A1

公开(公告)日：2016-08-04

申请号：US14917786

申请日：2014-09-09

Applicant: CHU SAINTE-JUSTINE

Inventor： Alain Moreau ,  Marie-Yvonne Akoume Ndong

IPC: G01N33/68 ,  G01N33/487 ,  C12N15/113 ,  A61F5/02

CPC classification number: G01N33/6893 ,  A61F5/02 ,  A61F5/026 ,  A61K31/4045 ,  A61K31/713 ,  A61K33/04 ,  A61K38/19 ,  A61K45/06 ,  C12N15/1138 ,  C12N2310/14 ,  C12N2320/30 ,  G01N33/48728 ,  G01N2333/47 ,  G01N2333/726 ,  G01N2800/10 ,  G01N2800/50 ,  G01N2800/52 ,  A61K2300/00

Abstract: The present invention provides a method of predicting brace treatment outcome in a subject in need thereof comprising i) classifying the subject into functional group FG1, FG2 or FG3, wherein the classification enables the prediction of brace treatment outcome. Also provided are methods of determining risk of developing scoliosis, methods of treating Idiopathic scoliosis and kits for applying the methods.

Abstract translation: 本发明提供了一种在有需要的受试者中预测支架治疗结局的方法，包括：i）将受试者分为功能组FG1，FG2或FG3，其中所述分类使得能够预测支具治疗结果。 还提供了确定发展脊柱侧凸风险的方法，治疗特发性脊柱侧凸的方法和应用该方法的试剂盒。

公开(公告)号：US20160139146A1

公开(公告)日：2016-05-19

申请号：US14898766

申请日：2014-06-16

Applicant: CHU SAINTE-JUSTINE

Inventor： Alain Moreau ,  Marie-Yvonne Akoume Ndong

IPC: G01N33/68

CPC classification number: G01N33/6872 ,  A61K31/18 ,  A61K31/4188 ,  A61K31/4427 ,  A61K31/4745 ,  G01N33/6893 ,  G01N2333/4704 ,  G01N2440/14 ,  G01N2800/10 ,  G01N2800/38 ,  G01N2800/50 ,  G01N2800/52

Abstract: Methods of stratifying a subject having or at risk for developing adolescent idiopathic scoliosis (AIS) into diagnostically or clinically useful subclasses are provided. The stratification is based on the subject's Giα protein serine phosphorylation profile and/or the degree of imbalance in G-protein coupled receptor responses to Giα and Gsα protein stimulation. In some embodiments, the methods involve detecting or determining the level of serine phosphorylated Giα1 and/or Giα3 proteins in the cell sample, and/or determining a ratio between the response to Giα protein stimulation and the response to Gsα protein stimulation from a biological sample from the subject. Methods for predicting the risk of an AIS subject for developing a severe scoliosis, for predicting the subject's responsiveness to bracing treatment, and for identifying therapeutically useful compounds are also provided, as well as kits therefor.

Abstract translation: 提供了将患有发展青少年特发性脊柱侧弯（AIS）或具有发展青少年特发性脊柱侧凸（AIS）的危险性的受试者分为诊断或临床有用的亚类的方法。 分层是基于受试者的Giα蛋白丝氨酸磷酸化谱和/或G-protein偶联受体对Giα和Gsα蛋白刺激的反应失衡程度。 在一些实施方案中，所述方法包括检测或确定细胞样品中丝氨酸磷酸化的Giα1和/或Giα3蛋白的水平，和/或确定对Giα蛋白刺激的反应与从生物样品对Gsα蛋白刺激的反应之间的比例 从主题。 还提供了用于预测AIS受试者发展严重脊柱侧凸的风险的方法，用于预测受试者对支架治疗的反应性以及用于鉴定治疗有用的化合物，以及用于其的试剂盒。

公开(公告)号：US20190195859A1

公开(公告)日：2019-06-27

申请号：US16327278

申请日：2017-08-23

Applicant: CHU Sainte-Justine

Inventor： Alain Moreau ,  Niaz Oliazadeh ,  Kristen Fay Gorman ,  Robert Eveleigh ,  Guillaume Bourque

IPC: G01N33/50 ,  C12Q1/6883

CPC classification number: G01N33/5005 ,  C07H21/04 ,  C12Q1/68 ,  C12Q1/6883 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/158 ,  C40B30/04 ,  C40B40/06 ,  G01N33/5044 ,  G01N2800/50

Abstract: Disclosed herein are novel molecular markers associated with idiopathic scoliosis (IS). Accordingly, the present invention concerns novel methods of identifying subjects at risk of developing IS or suffering from IS and of genotyping and classifying IS subjects into genetic and functional groups. Also provided are compositions, DNA chips and kits for applying the methods.

公开(公告)号：US20180224467A1

公开(公告)日：2018-08-09

申请号：US15910586

申请日：2018-03-02

Applicant: Chu Sainte-Justine

Inventor： Alain Moreau ,  Marie - Yvonne Akoume Ndong

IPC: G01N33/68 ,  A61F5/02 ,  C12N15/113 ,  A61K38/19 ,  A61K33/04 ,  A61K31/4045 ,  G01N33/487 ,  A61K45/06 ,  A61K31/713

CPC classification number: G01N33/6893 ,  A61F5/02 ,  A61F5/026 ,  A61K31/4045 ,  A61K31/713 ,  A61K33/04 ,  A61K38/19 ,  A61K45/06 ,  C12N15/1138 ,  C12N2310/14 ,  C12N2320/30 ,  G01N33/48728 ,  G01N2333/47 ,  G01N2333/726 ,  G01N2800/10 ,  G01N2800/50 ,  G01N2800/52 ,  A61K2300/00

Abstract: The present invention provides a method of treating a subject in need thereof comprising classifying the subject into functional group FG1, FG2 or FG3, wherein i) when the subject is classified into the FG1 functional group, (A) the level of OPN or the activity of OPN in said subject is increased; (B) the subject is not treated with a brace; or (C) a combination of (A) and (B); and ii) when the subject is classified into the FG2 or FG3 functional group, (A) the level of OPN or the activity of OPN in said subject is decreased; (B) the subject is treated with a brace; or (C) a combination of (A) and (B).

公开(公告)号：US20190091279A1

公开(公告)日：2019-03-28

申请号：US16082922

申请日：2016-03-09

Applicant: CHU SAINTE-JUSTINE ,  THE UNIVERSITY DE MONTREAL

Inventor： Sylvain CHEMTOB ,  Christiane QUINIOU ,  Sarah Anne ROBERTSON ,  Mathieu NADEAU-VALLÉE ,  William D. LUBELL ,  David OLSON ,  Peck Yin CHIN ,  Sylvie GIRARD ,  Doan-Ngoc DUC

IPC: A61K38/08 ,  A61P29/00

Abstract: A method for preventing or reducing the risk of perinatal or neonatal morbidity and mortality caused by antenatal inflammation in humans is described. This method is based on the administration of a compound of formula I or a pharmaceutically acceptable salt thereof to an expectant human mother suffering from antenatal fetal inflammation. The method prevents or reduces the risk of organ damages, including brain, lung and intestinal damages, and sequelae therefrom, in the neonates, as well as the risk of neonatal death.