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公开(公告)号：US08374474B2
公开(公告)日：2013-02-12
申请号：US12971088
申请日：2010-12-17
Applicant: Kuei-Huang Chou , Shing-wu Paul Tzeng , Chih-Yu Wu , Sheng-Hsiang Hsu
Inventor： Kuei-Huang Chou , Shing-wu Paul Tzeng , Chih-Yu Wu , Sheng-Hsiang Hsu
CPC classification number: G02B6/02395
Abstract: A structure for optical fiber with single layer coating suitable for field termination process is provided, including a glass core, a cladding layer, and a permanent coating protective layer. The thickness of the permanent coating ranges preferably from about 4 um to 8 um, and remains on the optical fiber during the field termination process to provide protection to the optical fiber after the buffer layer is striped off. In addition, the optical fiber structure of the present invention still conforms to the specification of the standard optical fiber. The optical fiber of the structure according to the present invention can simplify the field termination process so that the quality efficiency of the deployment is improved.
Abstract translation: 提供了适用于场终端工艺的具有单层涂层的光纤结构，包括玻璃芯，包覆层和永久性涂层保护层。 永久涂层的厚度优选为约4μm至8μm，并且在场终止处理期间保留在光纤上，以在缓冲层被剥离之后为光纤提供保护。 此外，本发明的光纤结构仍然符合标准光纤的规格。 根据本发明的结构的光纤可以简化现场终止处理，从而提高部署的质量效率。
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公开(公告)号：US20130001515A1
公开(公告)日：2013-01-03
申请号：US13346152
申请日：2012-01-09
Applicant: Lain-Jong Li , Ching-Yuan Su , Ang-Yu Lu , Chih-Yu Wu , Keng-Ku Liu
Inventor： Lain-Jong Li , Ching-Yuan Su , Ang-Yu Lu , Chih-Yu Wu , Keng-Ku Liu
IPC: H01L29/775 , C23C16/44 , B05D3/00 , B05D5/00 , B05D5/12 , H01L21/04 , B05D1/36 , B05D3/02 , B82Y40/00
CPC classification number: H01L21/0262 , B82Y30/00 , B82Y40/00 , C01B32/184 , H01L21/02381 , H01L21/0242 , H01L21/02422 , H01L21/02444 , H01L21/02488 , H01L21/02502 , H01L21/02527 , H01L21/02612
Abstract: Graphene layers can be formed on a dielectric substrate using a process that includes forming a copper thin film on a dielectric substrate; diffusing carbon atoms through the copper thin film; and forming a graphene layer at an interface between the copper thin film and the dielectric substrate.
Abstract translation: 可以使用包括在电介质基板上形成铜薄膜的工艺在电介质基板上形成石墨烯层; 通过铜薄膜扩散碳原子; 以及在所述铜薄膜和所述电介质基板之间的界面处形成石墨烯层。
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公开(公告)号：US20090213629A1
公开(公告)日：2009-08-27
申请号：US12098512
申请日：2008-04-07
Applicant: Hsing-Fu LIU , Chin Sun , Chih-Yu Wu
Inventor： Hsing-Fu LIU , Chin Sun , Chih-Yu Wu
IPC: H02M7/04
CPC classification number: H02M3/155 , H02M7/155 , H02M7/2176
Abstract: A powering circuit of an AC-DC converter, for converting a high AC input voltage into a low DC output voltage to provide a load voltage in a stable DC bias range, includes a rectifier, a sensing circuit, a control switching circuit, and a voltage regulating capacitor. The rectifier has a primary side coupled to an AC power supply and a secondary side for outputting a DC power supply. The sensing circuit compares the AC input voltage with a preset reference voltage, and turns on a second switch in the control switching circuit when the AC input voltage is lower than the reference voltage, thereby providing a low DC output voltage. The control switching circuit sustains the DC output voltage in a stable DC bias range. Therefore, in addition to reducing the power consumption of the second switch, this circuit structure is simple and can achieve the purpose of circuit integration.
Abstract translation: AC-DC转换器的供电电路，用于将高交流输入电压转换成低直流输出电压以提供稳定的直流偏置范围内的负载电压，包括整流器，感测电路，控制开关电路和 调压电容器。 整流器具有耦合到AC电源的初级侧和用于输出DC电源的次级侧。 感测电路将交流输入电压与预设参考电压进行比较，当交流输入电压低于参考电压时，接通控制开关电路中的第二开关，从而提供低直流输出电压。 控制开关电路在稳定的直流偏置范围内维持直流输出电压。 因此，除了降低第二开关的功耗外，该电路结构简单，可以达到电路集成的目的。
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公开(公告)号：US20080299653A1
公开(公告)日：2008-12-04
申请号：US11839500
申请日：2007-08-15
Applicant: Chung-Hsiung WANG , Chih-Yu Wu , Shih-Chia Yeh
Inventor： Chung-Hsiung WANG , Chih-Yu Wu , Shih-Chia Yeh
IPC: C12N5/06
CPC classification number: C12N5/0601
Abstract: The present invention relates to Lymantria xylina cell lines established from pupal tissues of L. xylina Swinhoe, including NTU-LY-1, NTU-LY-2, NTU-LY-3, and NTU-LY-4. These four cell lines were confirmed to be newly established cell lines derived from L. xylina by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) and isozyme analysis. The genotypes and characteristics of the abovementioned cell lines are totally different from other insect cell lines. In addition, these four L. xylina cell lines are susceptible to insect baculovirus of L. xylina multiple nucleopolyhedrovirus (LyxyMNPV), LdMNPV-like virus, PenuMNPV, as well as microsporidia of Nosema sp. (isolated from Eurema blanda) and N. bombycis and the like. Therefore the invention can be applied in multiplication of the abovementioned insect-pathogenic microorganisms to produce biopesticides in pest control. In addition, the cell lines can also be used as hosts for the expression vectors of said baculovirus to produce recombinant proteins.
Abstract translation: 本发明涉及由木霉菌Swinhoe的蛹组织构成的Lymantria xylina细胞系，包括NTU-LY-1，NTU-LY-2，NTU-LY-3和NTU-LY-4。 通过随机扩增多态性DNA聚合酶链反应（RAPD-PCR）和同工酶分析证实这四种细胞系是新建立的来自木霉菌的细胞系。 上述细胞系的基因型和特征与其他昆虫细胞系完全不同。 此外，这四种木霉素细胞系对L.xylina多核苷酸多角体病毒（LyxyMNPV），LdMNPV样病毒，PenuMNPV以及Nosema sp。的微孢子虫的昆虫杆状病毒敏感。 （分离自Eurema blanda）和N. bombycis等。 因此，本发明可以应用于上述昆虫病原微生物的繁殖，以在病虫害防治中产生生物杀虫剂。 此外，细胞系也可以用作所述杆状病毒表达载体的宿主以产生重组蛋白。
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公开(公告)号：US20070065924A1
公开(公告)日：2007-03-22
申请号：US11524428
申请日：2006-09-20
Applicant: Tzong-Yuan Wu , Chung-Hsiung Wang , Chih-Yu Wu , Ying-Ju Chen
Inventor： Tzong-Yuan Wu , Chung-Hsiung Wang , Chih-Yu Wu , Ying-Ju Chen
CPC classification number: C12N15/86 , C12N2770/32043 , C12N2830/00 , C12N2830/60 , C12N2840/203
Abstract: Provided herein is an isolated polynucleotide sequence with internal ribosome entry site (IRES) activity, which directs translation initiation in an insect expression system in a cap-independent manner. In particular, the invention relates to an isolated polynucleotide comprises the 5′ UTR of perina nuda Picorna-like virus (PnV) that possesses IRES activity. Methods of identifying a polynucleotide with IRES activity and methods of expressing at least two polypeptides in an insect system are also disclosed herein.
Abstract translation: 本文提供了具有内部核糖体进入位点（IRES）活性的分离的多核苷酸序列，其以帽独立的方式指导昆虫表达系统中的翻译起始。 特别地，本发明涉及包含具有IRES活性的perina nuda Picorna样病毒（PnV）的5'UTR的分离的多核苷酸。 鉴定具有IRES活性的多核苷酸的方法和在昆虫系统中表达至少两种多肽的方法也在本文中公开。
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Patent Agency Ranking
公开(公告)号：US08685843B2
公开(公告)日：2014-04-01
申请号：US13346152
申请日：2012-01-09
Applicant: Lain-Jong Li , Ching-Yuan Su , Ang-Yu Lu , Chih-Yu Wu , Keng-Ku Liu
Inventor： Lain-Jong Li , Ching-Yuan Su , Ang-Yu Lu , Chih-Yu Wu , Keng-Ku Liu
IPC: H01L21/04
CPC classification number: H01L21/0262 , B82Y30/00 , B82Y40/00 , C01B32/184 , H01L21/02381 , H01L21/0242 , H01L21/02422 , H01L21/02444 , H01L21/02488 , H01L21/02502 , H01L21/02527 , H01L21/02612
Abstract: Graphene layers can be formed on a dielectric substrate using a process that includes forming a copper thin film on a dielectric substrate; diffusing carbon atoms through the copper thin film; and forming a graphene layer at an interface between the copper thin film and the dielectric substrate.
Abstract translation: 可以使用包括在电介质基板上形成铜薄膜的工艺在电介质基板上形成石墨烯层; 通过铜薄膜扩散碳原子; 以及在所述铜薄膜和所述电介质基板之间的界面处形成石墨烯层。
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公开(公告)号：US20100223683A1
公开(公告)日：2010-09-02
申请号：US12778408
申请日：2010-05-12
Applicant: Tzong-Yuan Wu , Chung-Hsiung Wang , Chih-Yu Wu , Ying-Ju Chen
Inventor： Tzong-Yuan Wu , Chung-Hsiung Wang , Chih-Yu Wu , Ying-Ju Chen
IPC: A01K67/033 , C12N15/74 , C12N5/10
CPC classification number: C12N15/86 , C12N2770/32043 , C12N2830/00 , C12N2830/60 , C12N2840/203
Abstract: Provided herein is an isolated polynucleotide sequence with internal ribosome entry site (IRES) activity, which directs translation initiation in an insect expression system in a cap-independent manner. In particular, the invention relates to an isolated polynucleotide comprises the 5′ UTR of perina nuda Picorna-like virus (PnV) that possesses IRES activity. Methods of identifying a polynucleotide with IRES activity and methods of expressing at least two polypeptides in an insect system are also disclosed herein.
Abstract translation: 本文提供了具有内部核糖体进入位点（IRES）活性的分离的多核苷酸序列，其以帽独立的方式指导昆虫表达系统中的翻译起始。 特别地，本发明涉及包含具有IRES活性的perina nuda Picorna样病毒（PnV）的5'UTR的分离的多核苷酸。 鉴定具有IRES活性的多核苷酸的方法和在昆虫系统中表达至少两种多肽的方法也在本文中公开。
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公开(公告)号：US08088619B2
公开(公告)日：2012-01-03
申请号：US11969799
申请日：2008-01-04
Applicant: Chung-Hsiung Wang , Chih-Yu Wu , Song-Tay Lee
Inventor： Chung-Hsiung Wang , Chih-Yu Wu , Song-Tay Lee
CPC classification number: C12N5/0601
Abstract: The present invention relates to Maruca vitrata cell lines established from pupal tissues of M. vitrata, including NTU-MV, two subclones of NTU-MV: NTU-MV-1 and NTU-MV-2. The NTU-MV cell line was confirmed to be derived from M. vitrata, and NTU-MV-1, and NTU-MV-2 were confirmed to be derived from NTU-MV by karyotype analysis, Internal transcribed spacer (ITS) analysis and isozyme analysis. The genotypes and characteristics of the abovementioned 3 cell lines are totally different from other insect cell lines so that they are newly established cell lines. In addition, these three MV cell lines are susceptible to Maruca vitrata multiple nucleopolyhedrovirus (MaviMNPV). Therefore the 3 cell lines according to invention can be applied in multiplication of MaviMNPV in vitro to produce biopesticides in pest control. In addition, the cell lines can also be used as hosts for the expression vectors of baculovirus to produce recombinant proteins.
Abstract translation: 本发明涉及由薇皮菌的蛹组织构成的马拉维氏玻璃体细胞系，包括NTU-MV，NTU-MV：NTU-MV-1和NTU-MV-2的两个亚克隆。 证实NTU-MV细胞系来源于玻璃体，NTU-MV-1和NTU-MV-2通过核型分析，内转录间隔（ITS）分析和NTN-MV- 同工酶分析。 上述3种细胞系的基因型和特征与其他昆虫细胞系完全不同，因此它们是新建立的细胞系。 此外，这三种MV细胞系对Maruca vitrata多重核苷酸多角体病毒（MaviMNPV）敏感。 因此，根据本发明的3种细胞系可以在MaviMNPV的体外增殖中应用，以在病虫害防治中产生生物杀虫剂。 此外，细胞系也可以用作杆状病毒表达载体的宿主以产生重组蛋白。
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公开(公告)号：US20090098649A1
公开(公告)日：2009-04-16
申请号：US11969799
申请日：2008-01-04
Applicant: Chung-Hsiung Wang , Chih-Yu Wu , Song-Tay Lee
Inventor： Chung-Hsiung Wang , Chih-Yu Wu , Song-Tay Lee
IPC: C12N5/00
CPC classification number: C12N5/0601
Abstract: The present invention relates to Maruca vitrata cell lines established from pupal tissues of M. vitrata, including NTU-MV, two subclones of NTU-MV: NTU-MV-1 and NTU-MV-2. The NTU-MV cell line was confirmed to be derived from M. vitrata, and NTU-MV-1, and NTU-MV-2 were confirmed to be derived from NTU-MV by karyotype analysis, Internal transcribed spacer (ITS) analysis and isozyme analysis. The genotypes and characteristics of the abovementioned 3 cell lines are totally different from other insect cell lines so that they are newly established cell lines. In addition, these three MV cell lines are susceptible to Maruca vitrata multiple nucleopolyhedrovirus (MaviMNPV). Therefore the 3 cell lines according to invention can be applied in multiplication of MaviMNPV in vitro to produce biopesticides in pest control. In addition, the cell lines can also be used as hosts for the expression vectors of said baculovirus to produce recombinant proteins.
Abstract translation: 本发明涉及由薇皮菌的蛹组织构成的马拉维氏玻璃体细胞系，包括NTU-MV，NTU-MV：NTU-MV-1和NTU-MV-2的两个亚克隆。 证实NTU-MV细胞系来源于玻璃体，NTU-MV-1和NTU-MV-2通过核型分析，内转录间隔（ITS）分析和NTN-MV- 同工酶分析。 上述3种细胞系的基因型和特征与其他昆虫细胞系完全不同，因此它们是新建立的细胞系。 此外，这三种MV细胞系对Maruca vitrata多重核苷酸多角体病毒（MaviMNPV）敏感。 因此，根据本发明的3种细胞系可以在MaviMNPV的体外增殖中应用，以在病虫害防治中产生生物杀虫剂。 此外，细胞系也可以用作所述杆状病毒的表达载体的宿主以产生重组蛋白。
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公开(公告)号：US20120155818A1
公开(公告)日：2012-06-21
申请号：US12971088
申请日：2010-12-17
Applicant: Kuei-Huang CHOU , Shing-Wu Paul Tzeng , Chih-Yu Wu , Sheng-Hsiang Hsu
Inventor： Kuei-Huang CHOU , Shing-Wu Paul Tzeng , Chih-Yu Wu , Sheng-Hsiang Hsu
IPC: G02B6/02
CPC classification number: G02B6/02395
Abstract: A structure for optical fiber with single layer coating suitable for field termination process is provided, including a glass core, a cladding layer, and a permanent coating protective layer. The thickness of the permanent coating ranges preferably from about 4 um to 8 um, and remains on the optical fiber during the field termination process to provide protection to the optical fiber after the buffer layer is striped off. In addition, the optical fiber structure of the present invention still conforms to the specification of the standard optical fiber. The optical fiber of the structure according to the present invention can simplify the field termination process so that the quality efficiency of the deployment is improved.
Abstract translation: 提供了适用于场终端工艺的具有单层涂层的光纤结构，包括玻璃芯，包覆层和永久性涂层保护层。 永久涂层的厚度优选为约4μm至8μm，并且在场终止处理期间保留在光纤上，以在缓冲层被剥离之后为光纤提供保护。 此外，本发明的光纤结构仍然符合标准光纤的规格。 根据本发明的结构的光纤可以简化现场终止处理，从而提高部署的质量效率。
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