公开(公告)号：US06893822B2

公开(公告)日：2005-05-17

申请号：US09910469

申请日：2001-07-19

申请人： Markus Schweitzer ,  Richard R. Anderson ,  Michael D. Fiechtner ,  Jochen Müller ,  Stefan Raddatz ,  Christoph Brücher ,  Norbert Windhab ,  Jill M. Orwick ,  Eberhard Schneider ,  Marc Pignot ,  Stefan Kienle

发明人： Markus Schweitzer ,  Richard R. Anderson ,  Michael D. Fiechtner ,  Jochen Müller ,  Stefan Raddatz ,  Christoph Brücher ,  Norbert Windhab ,  Jill M. Orwick ,  Eberhard Schneider ,  Marc Pignot ,  Stefan Kienle

IPC分类号： G01N33/53 ,  B01J19/00 ,  B05D3/00 ,  C07H21/00 ,  C07H21/04 ,  C12M1/00 ,  C12M1/34 ,  C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  G01N33/543 ,  G01N33/566 ,  G01N37/00

CPC分类号： C07H21/00 ,  B01J19/0046 ,  B01J2219/00608 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00722 ,  C12Q1/6837 ,  G01N33/54353 ,  C12Q2565/518 ,  C12Q2525/117 ,  C12Q2525/113

摘要： The present invention relates to conjugates of synthetic binding units and nucleic acids. The present invention also relates to methods for sorting and immobilizing nucleic acids on support materials using such conjugates by specific molecular addressing of the nucleic acids mediated by the synthetic binding systems. Particularly, the present invention also relates to novel methods of utilizing conjugates of synthetic binding units and nucleic acids to in active electronic array systems to produce novel array constructs from the conjugates, and the use of such constructs in various nucleic acid assay formats. In addition, the present invention relates to various novel forms of such conjugates, improved methods of making solid phase synthesized conjugates, and improved methods of conjugating pre-synthesized synthetic binding units and nucleic acids. The present invention also relates to the use of conjugates of synthetic binding units and nucleic acids as substrates for various enzymatic reactions, including nucleic acid amplification reactions.

摘要翻译： 本发明涉及合成结合单元和核酸的缀合物。 本发明还涉及使用这种共轭物通过由合成结合系统介导的核酸的特异性分子寻址来分选和固定核酸的载体材料的方法。 特别地，本发明还涉及利用合成结合单元和核酸的缀合物在活性电子阵列体系中从缀合物产生新型阵列构建体的新方法，以及以各种核酸测定形式使用这种构建体。 此外，本发明涉及这种缀合物的各种新型形式，制备固相合成的缀合物的改进方法，以及与预合成的合成结合单元和核酸缀合的改进方法。 本发明还涉及合成结合单元和核酸的缀合物作为各种酶反应的底物的用途，包括核酸扩增反应。

公开(公告)号：US07129229B2

公开(公告)日：2006-10-31

申请号：US10344092

申请日：2001-08-10

申请人： Stefan Raddatz ,  Jochen Müller-Ibeler ,  Markus Schweitzer ,  Christoph Brücher ,  Norbert Windhab ,  John R. Havens ,  Thomas J. Onofrey ,  Charles H. Greef ,  Daguang Wang

发明人： Stefan Raddatz ,  Jochen Müller-Ibeler ,  Markus Schweitzer ,  Christoph Brücher ,  Norbert Windhab ,  John R. Havens ,  Thomas J. Onofrey ,  Charles H. Greef ,  Daguang Wang

IPC分类号： A61K31/66 ,  C07F9/02

CPC分类号： A61K31/66 ,  C07F9/2408 ,  C07F9/2429

摘要： This invention relates to attachment chemistries for binding macromolecules to a substrate surface or to other conjugation targets. More particularly, this invention relates to attachment chemistries involving branched or linear structures having one or more hydrazide attachment moieties for binding the macromolecules to a substrate surface, or for other conjugation reactions. Novel modifying reagents are provided for the introduction of protected hydrazide attachment moieties or precursor forms of such hydrazides to the macromolecule, either as a single hydrazide or as multiple hydrazides.

摘要翻译： 本发明涉及用于将大分子结合到底物表面或其它共轭靶的附着化学物质。 更具体地说，本发明涉及包含具有一个或多个用于将大分子结合到底物表面或其它共轭反应的一个或多个酰肼连接部分的支链或线性结构的附着化学物质。 提供了新的改性试剂，用于将受保护的酰肼连接部分或这种酰肼的前体形式引入高分子，作为单酰肼或多个酰肼。