公开(公告)号：US06893822B2

公开(公告)日：2005-05-17

申请号：US09910469

申请日：2001-07-19

申请人： Markus Schweitzer ,  Richard R. Anderson ,  Michael D. Fiechtner ,  Jochen Müller ,  Stefan Raddatz ,  Christoph Brücher ,  Norbert Windhab ,  Jill M. Orwick ,  Eberhard Schneider ,  Marc Pignot ,  Stefan Kienle

发明人： Markus Schweitzer ,  Richard R. Anderson ,  Michael D. Fiechtner ,  Jochen Müller ,  Stefan Raddatz ,  Christoph Brücher ,  Norbert Windhab ,  Jill M. Orwick ,  Eberhard Schneider ,  Marc Pignot ,  Stefan Kienle

IPC分类号： G01N33/53 ,  B01J19/00 ,  B05D3/00 ,  C07H21/00 ,  C07H21/04 ,  C12M1/00 ,  C12M1/34 ,  C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  G01N33/543 ,  G01N33/566 ,  G01N37/00

CPC分类号： C07H21/00 ,  B01J19/0046 ,  B01J2219/00608 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00722 ,  C12Q1/6837 ,  G01N33/54353 ,  C12Q2565/518 ,  C12Q2525/117 ,  C12Q2525/113

摘要： The present invention relates to conjugates of synthetic binding units and nucleic acids. The present invention also relates to methods for sorting and immobilizing nucleic acids on support materials using such conjugates by specific molecular addressing of the nucleic acids mediated by the synthetic binding systems. Particularly, the present invention also relates to novel methods of utilizing conjugates of synthetic binding units and nucleic acids to in active electronic array systems to produce novel array constructs from the conjugates, and the use of such constructs in various nucleic acid assay formats. In addition, the present invention relates to various novel forms of such conjugates, improved methods of making solid phase synthesized conjugates, and improved methods of conjugating pre-synthesized synthetic binding units and nucleic acids. The present invention also relates to the use of conjugates of synthetic binding units and nucleic acids as substrates for various enzymatic reactions, including nucleic acid amplification reactions.

摘要翻译： 本发明涉及合成结合单元和核酸的缀合物。 本发明还涉及使用这种共轭物通过由合成结合系统介导的核酸的特异性分子寻址来分选和固定核酸的载体材料的方法。 特别地，本发明还涉及利用合成结合单元和核酸的缀合物在活性电子阵列体系中从缀合物产生新型阵列构建体的新方法，以及以各种核酸测定形式使用这种构建体。 此外，本发明涉及这种缀合物的各种新型形式，制备固相合成的缀合物的改进方法，以及与预合成的合成结合单元和核酸缀合的改进方法。 本发明还涉及合成结合单元和核酸的缀合物作为各种酶反应的底物的用途，包括核酸扩增反应。

公开(公告)号：US4912208A

公开(公告)日：1990-03-27

申请号：US67833

申请日：1987-06-29

申请人： Michael D. Fiechtner ,  Christopher Bieniarz ,  Mohamed Shipchandler ,  Maciej Adamczyk

发明人： Michael D. Fiechtner ,  Christopher Bieniarz ,  Mohamed Shipchandler ,  Maciej Adamczyk

IPC分类号： G01N33/544 ,  C07D493/10 ,  C07H15/26 ,  G01N33/542 ,  G01N33/543 ,  G01N33/58

CPC分类号： C07D493/10 ,  B82Y5/00 ,  C07H15/26 ,  G01N33/5432 ,  G01N33/586

摘要： The present invention provides novel fluorescein derivative compounds having fluorescence spectrum and quantum yield characteristics similar to those of fluorescein. The compounds are readily synthesized and purified and are readily soluble in water at self-quenching concentrations. Significantly, due to the presence of polar polyhydroxy group substituents and the absence of metal-chelating groups, these fluorescein derivatives are susceptible to minimal leakage across liposome membranes and have fluorescence characteristics minimally sensitive to the presence of metal ions. Compounds of the invention are thus exceptionally suitable for use in the development of highly storage stable liposome preparations to be employed in immunolytic assays involving human body fluid samples.

公开(公告)号：US5686316A

公开(公告)日：1997-11-11

申请号：US431398

申请日：1995-04-28

申请人： Michael D. Fiechtner ,  John M. Ramp ,  Barbara J. England ,  Mary J. Annino

发明人： Michael D. Fiechtner ,  John M. Ramp ,  Barbara J. England ,  Mary J. Annino

IPC分类号： G01N33/53 ,  G01N33/531 ,  G01N33/543 ,  G01N33/72 ,  G01N33/96 ,  G01N37/00 ,  G01N33/537 ,  G01N33/538 ,  G01N33/545

CPC分类号： G01N33/723 ,  G01N33/54353 ,  G01N33/96 ,  Y10S435/975 ,  Y10S436/805 ,  Y10S436/826 ,  Y10S436/827 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/105831 ,  Y10T436/106664 ,  Y10T436/25625

摘要： The invention is a rapid, continuous test for glycated hemoglobin using a non-equilibrium affinity binding method. Agarose beads derivatized with 3-aminophenylboronic acid specifically bind glycated hemoglobin. This solid phase is incorporated into a sample processor card, modified to mix and to separate the test solution from the solid phase prior to absorbance readings. Two absorbance readings are made on the test solution, one immediately after mixing the reagent/diluent with the specimen, and one after a significant amount of binding has occurred. A linear correlation between total glycated hemoglobin and hemoglobin A.sub.1c permits standardization and reporting of units equivalent to % hemoglobin A.sub.1c. Stable glycated hemoglobin solutions for use as standards in the assay, and a method for preparing the standards are also disclosed.

摘要翻译： 本发明是使用非平​​衡亲和力结合方法对糖化血红蛋白的快速连续测试。 用3-氨基苯基硼酸衍生的琼脂糖珠特异性结合糖化血红蛋白。 该固相被并入样品处理器卡中，在吸光度读数之前被修改以混合并将测试溶液与固相分离。 在测试溶液上进行两次吸光度读数，一次在试剂/稀释剂与样品混合后立即进行，并且发生了大量的结合之后。 总糖化血红蛋白和血红蛋白A1c之间的线性相关性允许标准化和报告相当于％血红蛋白A1c的单位。 还公开了用作测定中的标准物的稳定的糖化血红蛋白溶液和制备标准品的方法。

公开(公告)号：US5589393A

公开(公告)日：1996-12-31

申请号：US427508

申请日：1995-04-24

申请人： Michael D. Fiechtner ,  John M. Ramp ,  Barbara J. England ,  Mary J. Annino

发明人： Michael D. Fiechtner ,  John M. Ramp ,  Barbara J. England ,  Mary J. Annino

IPC分类号： G01N33/53 ,  G01N33/531 ,  G01N33/543 ,  G01N33/72 ,  G01N33/96 ,  G01N37/00

CPC分类号： G01N33/723 ,  G01N33/54353 ,  G01N33/96 ,  Y10S435/975 ,  Y10S436/805 ,  Y10S436/826 ,  Y10S436/827 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/105831 ,  Y10T436/106664 ,  Y10T436/25625

摘要： The invention is a rapid, continuous test for glycated hemoglobin using a non-equilibrium affinity binding method. Agarose beads derivatized with 3-aminophenylboronic acid specifically bind glycated hemoglobin. This solid phase is incorporated into a sample processor card, modified to mix and to separate the test solution from the solid phase prior to absorbance readings. Two absorbance readings are made on the test solution, one immediately after mixing the reagent/diluent with the specimen, and one after a significant amount of binding has occurred. A linear correlation between total glycated hemoglobin and hemoglobin A.sub.1c permits standardization and reporting of units equivalent to % hemoglobin A.sub.1c. Stable glycated hemoglobin solutions for use as standards in the assay, and a method for preparing the standards are also disclosed.

摘要翻译： 本发明是使用非平​​衡亲和力结合方法对糖化血红蛋白的快速连续测试。 用3-氨基苯基硼酸衍生的琼脂糖珠特异性结合糖化血红蛋白。 该固相被并入样品处理器卡中，在吸光度读数之前被修改以混合并将测试溶液与固相分离。 在测试溶液上进行两次吸光度读数，一次在试剂/稀释剂与样品混合后立即进行，并且发生了大量的结合之后。 总糖化血红蛋白和血红蛋白A1c之间的线性相关性允许标准化和报告相当于％血红蛋白A1c的单位。 还公开了用作测定中的标准物的稳定的糖化血红蛋白溶液和制备标准品的方法。

公开(公告)号：US4970074A

公开(公告)日：1990-11-13

申请号：US367151

申请日：1989-07-24

申请人： Michael D. Fiechtner ,  Christopher Bieniarz ,  Mohamed Shipchandler ,  Maciej Adamczyk

发明人： Michael D. Fiechtner ,  Christopher Bieniarz ,  Mohamed Shipchandler ,  Maciej Adamczyk

IPC分类号： A61K9/127 ,  C07D493/10 ,  C07H15/26 ,  G01N33/543 ,  G01N33/58

CPC分类号： C07D493/10 ,  A61K9/127 ,  C07H15/26 ,  G01N33/5432 ,  G01N33/586

摘要： The present invention provides novel fluorescein derivative compounds having fluorescence spectrum and quantum yield characteristics similar to those of fluorescein. The compounds are readily synthesized and purified and are readily soluble in water at self-quenching concentrations. Significantly, due to the presence of polar polyhydroxy group substituents and the absence of metal-chelating groups, these fluorescein derivatives are susceptible to minimal leakage across liposome membranes and have fluorescence characteristics minimally sensitive to the presence of metal ions. Compounds of the invention are thus exceptionally suitable for use in the development of highly storage stable liposome preparations to be employed in immunolytic assays involving human body fluid samples.

摘要翻译： 本发明提供具有与荧光素相似的荧光光谱和量子产率特征的新型荧光素衍生物化合物。 化合物容易合成和纯化，并且在自熄浓度下易溶于水。 显着地，由于极性多羟基取代基的存在和不存在金属螯合基团，这些荧光素衍生物易受到脂质体膜上的最小渗漏的影响，并且具有对金属离子存在最不敏感的荧光特性。 因此，本发明的化合物特别适用于开发用于涉及人体液样品的免疫分析测定中的高度储存稳定的脂质体制剂。