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    Stable liquid composition comprising botulinum toxin
    2.
    发明授权
    Stable liquid composition comprising botulinum toxin 有权

    公开(公告)号：US11185503B2

    公开(公告)日：2021-11-30

    申请号：US16477889

    申请日：2017-10-17

    申请人： DAEWOONG CO., LTD.

    发明人： Hyeona Yim ,  Cheong-Sei Kim

    IPC分类号： A61K9/08 ,  A61K38/48 ,  A61K9/00 ,  A61K47/18 ,  A61K47/38 ,  A61K47/36 ,  A61K47/26

    摘要： A stable liquid composition comprising a botulinum toxin as an active ingredient is described, which is ready-to-use without the need for a reconstitution process, and thus, it is possible to improve user's convenience, and reduce a deviation of botulinum toxin activity due to dilution errors in the reconstitution process. Further, the liquid composition efficiently prevents the botulinum toxin from aggregating even at a low botulinum toxin concentration to thereby have extremely excellent storage stability, and efficiently prevents adsorption of the botulinum toxin to a container, thereby constantly maintaining an activity of the botulinum toxin for each batch or for each liquid vial.

    Method for production of botulinum toxin
    4.
    发明授权
    Method for production of botulinum toxin 有权
    生产肉毒杆菌毒素的方法

    公开(公告)号：US09512418B2

    公开(公告)日：2016-12-06

    申请号：US14654819

    申请日：2014-05-07

    申请人： DAEWOONG Co., LTD.

    发明人： Chung Sei Kim ,  Kwan Young Song ,  Kyoung Min Min ,  Yeong Duk An

    IPC分类号： C07K14/33 ,  C12N9/52

    CPC分类号： C12N9/52 ,  C07K14/33 ,  C12Y304/24069

    摘要： A method of making botulinum toxin comprises treating a culture of a botulinum toxin-producing strain with acid to precipitate a botulinum toxin; adding buffer to the precipitated botulinum toxin, followed by treatment with a protease inhibitor and nuclease, thereby extracting the botulinum toxin; treating the extracted botulinum toxin with acid to precipitate the botulinum toxin and dissolving the precipitate in buffer; and purifying the botulinum toxin by anion exchange chromatography. The use of the method makes it possible to produce a high-purity botulinum toxin by a simple process. The botulinum toxin produced by the method has high purity, and thus has an increased ability to act in a local area. Thus, the systemic circulation of the botulinum toxin is reduced to increase the safety. Accordingly, the botulinum toxin can be used for treatment of neuromuscular disorders, removal of wrinkles, and treatment of spastic hemiplegia and cerebral palsy.

    摘要翻译： 制备肉毒毒素的方法包括用酸处理产生肉毒杆菌毒素菌株的培养物以沉淀肉毒杆菌毒素; 向沉淀的肉毒杆菌毒素中加入缓冲液，然后用蛋白酶抑制剂和核酸酶处理，从而提取肉毒杆菌毒素; 用酸处理提取的肉毒毒素以沉淀肉毒杆菌毒素并将沉淀溶解在缓冲液中; 并通过阴离子交换色谱法纯化肉毒杆菌毒素。 使用该方法可以通过简单的方法生产高纯度肉毒杆菌毒素。 通过该方法产生的肉毒杆菌毒素具有高纯度，因此具有增强的局部作用能力。 因此，肉毒杆菌毒素的全身循环减少以增加安全性。 因此，肉毒杆菌毒素可用于治疗神经肌肉疾病，去除皱纹，以及治疗痉挛性偏瘫和脑瘫。

    Medium composition for preparing botulinum toxin
    5.
    发明授权
    Medium composition for preparing botulinum toxin 有权

    公开(公告)号：US10465179B2

    公开(公告)日：2019-11-05

    申请号：US15893297

    申请日：2018-02-09

    申请人： DAEWOONG CO., LTD.

    发明人： Kyoung-Yun Kim ,  Hye-Young Sul ,  Kyoung-Min Min

    IPC分类号： C12N1/20 ,  C12N9/52 ,  C07K14/33 ,  C12N5/00

    摘要： The present invention relates to a medium composition for production of botulinum toxin and, more particularly, to a medium composition for culture of Clostridium sp. capable of producing botulinum toxin. The medium composition of the present invention comprises at least one plant-derived peptone selected from the group consisting of a garden pea hydrolysate, a cotton seed hydrolysate and a wheat gluten hydrolysate. When the medium according to the present invention, which contains plant-derived peptones and minerals, is used for culture of Clostridium botulinum, the growth rate of the bacterium in the medium is about 1.5-2 times higher than that in the medium that is in current use. In addition, when botulinum toxin is produced by culturing the bacterium in the medium, infection with transmissible spongiform encephalopathy (TSE) or the like can be prevented by blocking introduction of animal-derived components.

    Medium composition for preparing botulinum toxin
    6.
    发明授权
    Medium composition for preparing botulinum toxin 有权

    公开(公告)号：US09926549B2

    公开(公告)日：2018-03-27

    申请号：US15521817

    申请日：2016-04-28

    申请人： DAEWOONG CO., LTD.

    发明人： Kyoung-Yun Kim ,  Hye-Young Sul ,  Kyoung-Min Min

    IPC分类号： C12N1/20 ,  C12N9/52

    CPC分类号： C12N9/52 ,  C07K14/33 ,  C12N1/20 ,  C12N5/0025 ,  C12N2500/76 ,  C12Y304/24069

    摘要： The present invention relates to a medium composition for production of botulinum toxin and, more particularly, to a medium composition for culture of Clostridium sp. capable of producing botulinum toxin. The medium composition of the present invention comprises at least one plant-derived peptone selected from the group consisting of a garden pea hydrolysate, a cotton seed hydrolysate and a wheat gluten hydrolysate. When the medium according to the present invention, which contains plant-derived peptones and minerals, is used for culture of Clostridium botulinum, the growth rate of the bacterium in the medium is about 1.5-2 times higher than that in the medium that is in current use. In addition, when botulinum toxin is produced by culturing the bacterium in the medium, infection with transmissible spongiform encephalopathy (TSE) or the like can be prevented by blocking introduction of animal-derived components.

    Medium composition for preparing botulinum toxin
    9.
    发明授权
    Medium composition for preparing botulinum toxin 有权

    公开(公告)号：US10117927B2

    公开(公告)日：2018-11-06

    申请号：US15521833

    申请日：2016-04-28

    申请人： DAEWOONG CO., LTD.

    发明人： Kyoung-Yun Kim ,  Hye-Young Sul ,  Kyoung-Min Min

    IPC分类号： A61K39/385 ,  A61K39/39 ,  C12N1/20

    摘要： The present invention relates to a medium composition for production of botulinum toxin and, more particularly, to a medium composition for culture of Clostridium sp. capable of producing botulinum toxin. The medium composition of the present invention comprises a casein hydrolysate and at least one plant-derived peptone selected from the group consisting of a garden pea hydrolysate, a cotton seed hydrolysate and a wheat gluten hydrolysate. When the medium according to the present invention, which contains plant-derived peptones, casein hydrolysates and minerals, is used for culture of Clostridium botulinum, the growth rate of the bacterium in the medium is higher than that in each of the medium that is in current use and the medium comprising plant-derived peptones alone. In addition, when the medium of the present invention is used, a high concentration of botulinum toxin can be produced by culturing the bacterium in a safe manner.

    MEDIUM COMPOSITION FOR PREPARING BOTULINUM TOXIN
    10.
    发明申请
    MEDIUM COMPOSITION FOR PREPARING BOTULINUM TOXIN 审中-公开

    公开(公告)号：US20170252431A1

    公开(公告)日：2017-09-07

    申请号：US15521833

    申请日：2016-04-28

    申请人： DAEWOONG CO., LTD.

    发明人： Kyoung-Yun Kim ,  Hye-Young Sul ,  Kyoung-Min Min

    IPC分类号： A61K39/385 ,  C12N1/20 ,  A61K39/39

    CPC分类号： A61K39/385 ,  A61K39/39 ,  C07K14/33 ,  C12N1/20

    摘要： The present invention relates to a medium composition for production of botulinum toxin and, more particularly, to a medium composition for culture of Clostridium sp. capable of producing botulinum toxin. The medium composition of the present invention comprises a casein hydrolysate and at least one plant-derived peptone selected from the group consisting of a garden pea hydrolysate, a cotton seed hydrolysate and a wheat gluten hydrolysate. When the medium according to the present invention, which contains plant-derived peptones, casein hydrolysates and minerals, is used for culture of Clostridium botulinum, the growth rate of the bacterium in the medium is higher than that in each of the medium that is in current use and the medium comprising plant-derived peptones alone. In addition, when the medium of the present invention is used, a high concentration of botulinum toxin can be produced by culturing the bacterium in a safe manner.