利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

12 条记录 (耗时 0.016 秒)

    Method of determining sugar content of tobacco using a discrete analyzer
    5.
    发明授权
    Method of determining sugar content of tobacco using a discrete analyzer 失效
    使用离散分析仪测定烟草糖含量的方法

    公开(公告)号：US4777131A

    公开(公告)日：1988-10-11

    申请号：US2933

    申请日：1987-01-13

    申请人： Terence M. Long ,  David J. Newman ,  Susan J. Alsop

    发明人： Terence M. Long ,  David J. Newman ,  Susan J. Alsop

    IPC分类号： C12Q1/34 ,  C12Q1/54 ,  G01N31/22 ,  C12Q1/32 ,  C12Q1/42 ,  C12Q1/48

    CPC分类号： G01N31/22 ,  C12Q1/34 ,  C12Q1/54 ,  G01N2333/904 ,  G01N2333/9121 ,  Y10T436/200833 ,  Y10T436/201666 ,  Y10T436/203332

    摘要： A method of determining in a discrete analyzer the total sugars content of a tobacco sample containing sucrose, glucose and fructose, comprises the steps of, converting the sucrose to glucose and fructose, enzymatically converting the glucose and fructose to glucose-6-phosphate in the presence of hexokinase, reacting the glucose-6-phosphate with nicotinamide-adenine dinucleotide in the presence of glucose-6-phosphate dehydrogenase to give reduced nicotinamide-adenine dinucleotide, the amount of reduced nicotinamide-adenine dinucleotide produced being directly proportional to the total sugars content of the sample, measuring the concentration of reduced nicotinamide-adenine dinucleotide in the sample, and generating a signal indicative of the concentration of reduced nicotinamide-adenine dinucleotide and hence of the total sugars content of the sample. Deactivation of enzymes by any cyanogen bromide that may be present is prevented by the addition of dithioerythritol to the reaction mixture.

    摘要翻译： 在离散分析仪中测定含有蔗糖，葡萄糖和果糖的烟草样品的总糖含量的方法包括以下步骤：将蔗糖转化为葡萄糖和果糖，在葡萄糖和果糖中将葡萄糖和果糖转化为葡萄糖-6-磷酸 己糖激酶的存在，在葡萄糖-6-磷酸脱氢酶存在下使葡萄糖-6-磷酸与烟酰胺 - 腺嘌呤二核苷酸反应，得到还原的烟酰胺 - 腺嘌呤二核苷酸，产生的还原烟酰胺 - 腺嘌呤二核苷酸的量与总糖成正比 样品的含量，测量样品中还原型烟酰胺 - 腺嘌呤二核苷酸的浓度，并产生指示还原型烟酰胺 - 腺嘌呤二核苷酸浓度以及因此样品总糖含量的信号。 通过向反应混合物中加入二硫代赤藓糖可以防止可能存在的任何溴化氰对酶的失活。

    Pure cultures of Kibdelsporangium aridum Shearer gen. nov., sp. nov. ATCC 39323 and mutants thereof
    7.
    发明授权
    Pure cultures of Kibdelsporangium aridum Shearer gen. nov., sp. nov. ATCC 39323 and mutants thereof 失效
    Kibdelsporangium aridum Shearer gen的纯文化。 诺夫山脉 nov。 ATCC 39323及其突变体

    公开(公告)号：US4672036A

    公开(公告)日：1987-06-09

    申请号：US749029

    申请日：1985-06-26

    申请人： Betty A. Bowie ,  David J. Newman ,  Marcia C. Shearer

    发明人： Betty A. Bowie ,  David J. Newman ,  Marcia C. Shearer

    IPC分类号： C12P1/06 ,  C12N1/14 ,  C12R1/645

    CPC分类号： C12R1/04 ,  C12P1/06 ,  Y10S435/911

    摘要： A novel antibiotic complex of "vancomycin-like" antibiotics, AAD 216 complex, is produced by the cultivation of a fermentation broth containing Kibdelosporangium aridum Shearer gen. nov., sp. nov. ATCC 39323 microorganisms in an aqueous nutrient medium under submerged aerobic conditions. The AAD 216 complex and its major bioactive components; AAD 216A, AAD 216B, and AAD 216C exhibit antibiotic activity and growth promotant activity.

    摘要翻译： 通过培养含有Kibdelosporangium aridum Shearer gen的发酵液，产生“万古霉素样”抗生素AAD216复合物的新型抗生素复合物。 诺夫山脉 nov。 ATCC 39323微生物在含水营养培养基中浸没有氧条件。 AAD 216复合物及其主要生物活性成分; AAD 216A，AAD 216B和AAD 216C表现出抗生素活性和生长促进剂活性。