公开(公告)号：US5630924A

公开(公告)日：1997-05-20

申请号：US425828

申请日：1995-04-20

申请人： Martin Fuchs ,  Wassim A. Nashabeh ,  Dieter R. Schmalzing

发明人： Martin Fuchs ,  Wassim A. Nashabeh ,  Dieter R. Schmalzing

IPC分类号： G01N33/533 ,  C12Q1/68 ,  G01N27/447 ,  G01N33/537 ,  G01N33/561 ,  G01N33/563 ,  G01N37/00 ,  C25B7/00

CPC分类号： G01N27/44726 ,  C12Q1/68 ,  G01N33/5375 ,  G01N33/561 ,  G01N2333/59

摘要： Compositions, methods, and apparatus for performing ultrafast binding assays by capillary electrophoresis or other electroseparation techniques are disclosed. In one embodiment, a first binding partner carries a detectable label and a second binding partner is modified to be highly charged. When used in combination with a sample containing an analyte with which both binding partners can interact and bind thereto, a three-membered complex is formed. The electrophoretic mobility difference between the unbound and complex-bound forms of labeled first binding partner is such that electroseparation and subsequent detection of an analyte can be accomplished. The compositions, methods, and apparatus disclosed herein also permit quantitative determination of the concentration of an analyte in a sample.

摘要翻译： 公开了通过毛细管电泳或其他电离分离技术进行超快速结合测定的组合物，方法和装置。 在一个实施方案中，第一结合伴侣携带可检测标记，第二结合伴侣被修饰为高度带电。 当与含有两个结合配偶体可以与其结合的分析物的样品组合使用时，形成三元复合物。 标记的第一结合配偶体的未结合和复合结合形式之间的电泳迁移率差异可以实现电分离和随后的分析物检测。 本文公开的组合物，方法和装置还允许定量测定样品中分析物的浓度。

公开(公告)号：US06991713B2

公开(公告)日：2006-01-31

申请号：US09768075

申请日：2001-01-23

申请人： Aram S. Adourian ,  Daniel J. Ehrlich ,  Lance B. Koutny ,  Paul T. Matsudaira ,  Dieter R. Schmalzing

发明人： Aram S. Adourian ,  Daniel J. Ehrlich ,  Lance B. Koutny ,  Paul T. Matsudaira ,  Dieter R. Schmalzing

IPC分类号： G01N27/26 ,  G01N27/447

CPC分类号： G01N27/44743 ,  B01L3/0275 ,  B01L3/0289 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/0657 ,  B01L2300/0803 ,  B01L2300/0816 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/043 ,  B01L2400/0442 ,  G01N27/44782 ,  G01N35/10 ,  Y10T436/2575

摘要： A technique processes a sample of biomolecular analyte. The technique uses an apparatus having a support assembly that receives and supports a test module, a load assembly that loads the sample of biomolecular analyte onto the test module, an electrophoresis assembly that applies a current to the test module such that components within the sample separate by electrophoresis, and a controller that controls operations of the load assembly and the electrophoresis assembly. The load assembly and the electrophoresis assembly are coupled to the support assembly. The controller controls the operation of the load assembly in an automated manner. Preferably, the test module includes a dielectric plate member having an upper planar surface and a lower planar surface that is spaced apart from and coplanar with the upper planar surface. The dielectric plate member has at least one set of channels that includes an injection channel and a separation channel. The injection channel extends from the upper planar surface to the lower planar surface. The separation channel extends within the dielectric plate member in a plane parallel with the upper and lower planar surfaces and intersects the injection channel.

摘要翻译： 一种技术处理生物分子分析物的样品。 该技术使用具有支撑组件的装置，其接收和支撑测试模块，将生物分子分析物的样品加载到测试模块上的负载组件，电泳组件，其将电流施加到测试模块，使得样品中的组分分离 通过电泳，以及控制负载组件和电泳组件的操作的控制器。 负载组件和电泳组件联接到支撑组件。 控制器以自动方式控制负载组件的操作。 优选地，测试模块包括具有上平坦表面和与上平坦表面间隔开并与之共面的下平面表面的电介质板构件。 电介质板构件具有包括注入通道和分离通道的至少一组通道。 注入通道从上平面延伸到下平面。 分离通道在平坦于上平面和下平面的平面内在电介质板构件内延伸，并与注入通道相交。

公开(公告)号：US06207031B1

公开(公告)日：2001-03-27

申请号：US09153215

申请日：1998-09-14

申请人： Aram S. Adourian ,  Daniel J. Ehrlich ,  Lance B. Koutny ,  Paul T. Matsudaira ,  Dieter R. Schmalzing

发明人： Aram S. Adourian ,  Daniel J. Ehrlich ,  Lance B. Koutny ,  Paul T. Matsudaira ,  Dieter R. Schmalzing

IPC分类号： G01N2726

CPC分类号： G01N27/44743 ,  B01L3/0275 ,  B01L3/0289 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/0657 ,  B01L2300/0803 ,  B01L2300/0816 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/043 ,  B01L2400/0442 ,  G01N27/44782 ,  G01N35/10 ,  Y10T436/2575

摘要： A technique processes a sample of biomolecular analyte. The technique uses an apparatus having a support assembly that receives and supports a test module, a load assembly that loads the sample of biomolecular analyte onto the test module, an electrophoresis assembly that applies a current to the test module such that components within the sample separate by electrophoresis, and a controller that controls operations of the load assembly and the electrophoresis assembly. The load assembly and the electrophoresis assembly are coupled to the support assembly. The controller controls the operation of the load assembly in an automated manner. Preferably, the test module includes a dielectric plate member having an upper planar surface and a lower planar surface that is spaced apart from and coplanar with the upper planar surface. The dielectric plate member has at least one set of channels that includes an injection channel and a separation channel. The injection channel extends from the upper planar surface to the lower planar surface. The separation channel extends within the dielectric plate member in a plane parallel with the upper and lower planar surfaces and intersects the injection channel.

摘要翻译： 一种技术处理生物分子分析物的样品。 该技术使用具有支撑组件的装置，其接收和支撑测试模块，将生物分子分析物的样品加载到测试模块上的负载组件，电泳组件，其将电流施加到测试模块，使得样品中的组分分离 通过电泳，以及控制负载组件和电泳组件的操作的控制器。 负载组件和电泳组件联接到支撑组件。 控制器以自动方式控制负载组件的操作。 优选地，测试模块包括具有上平坦表面和与上平坦表面间隔开并与之共面的下平面表面的电介质板构件。 电介质板构件具有包括注入通道和分离通道的至少一组通道。 注入通道从上平面延伸到下平面。 分离通道在平坦于上平面和下平面的平面内在电介质板构件内延伸，并与注入通道相交。

公开(公告)号：US5948231A

公开(公告)日：1999-09-07

申请号：US853031

申请日：1997-05-08

申请人： Martin Fuchs ,  Wassim A. Nashabeh ,  Dieter R. Schmalzing

发明人： Martin Fuchs ,  Wassim A. Nashabeh ,  Dieter R. Schmalzing

IPC分类号： G01N33/533 ,  C12Q1/68 ,  G01N27/447 ,  G01N33/537 ,  G01N33/561 ,  G01N33/563 ,  G01N37/00 ,  G01N27/26

CPC分类号： G01N27/44726 ,  C12Q1/68 ,  G01N33/5375 ,  G01N33/561 ,  G01N2333/59

摘要： Compositions, methods, and apparatus for performing ultrafast binding assays by capillary electrophoresis or other electroseparation techniques are disclosed. In one embodiment, a first binding partner carries a detectable label and a second binding partner is modified to be highly charged. When used in combination with a sample containing an analyte with which both binding partners can interact and bind thereto, a three-membered complex is formed. The electrophoretic mobility difference between the unbound and complex-bound forms of labeled first binding partner is such that electroseparation and subsequent detection of an analyte can be accomplished. The compositions, methods, and apparatus disclosed herein also permit quantitative determination of the concentration of an analyte in a sample.

摘要翻译： 公开了通过毛细管电泳或其它电离分离技术进行超快速结合测定的组合物，方法和装置。 在一个实施方案中，第一结合伴侣携带可检测标记，第二结合伴侣被修饰为高度带电。 当与含有两个结合配偶体可以与其结合的分析物的样品组合使用时，形成三元复合物。 标记的第一结合配偶体的未结合和复合结合形式之间的电泳迁移率差异可以实现电分离和随后的分析物检测。 本文公开的组合物，方法和装置还允许定量测定样品中分析物的浓度。