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1.发明授权YLMPO1 gene derived from yarrowia lipolytica and a process for preparing a glycoprotein not being mannosylphosphorylated by using a mutated yarrowia lipolytica in which YLMPO1 gene is disrupted 有权衍生自解脂耶氏酵母的YLMPO1基因和通过使用其中YLMPO1基因被破坏的突变的解脂耶氏酵母的解脂耶氏酵母制备不被甘露糖基磷酸化的糖蛋白的方法公开(公告)号：US08003349B2
公开(公告)日：2011-08-23
申请号：US12598887
申请日：2007-11-30
申请人： Jeong-Nam Park , Yunkyoung Song , Jeong-Yoon Kim , Doo-Byoung Oh , Hyun Ah Kang
发明人： Jeong-Nam Park , Yunkyoung Song , Jeong-Yoon Kim , Doo-Byoung Oh , Hyun Ah Kang
CPC分类号： C12N15/815 , C12N9/1081
摘要： The present invention relates to a novel YlMPO1 gene which plays an important role in mannosylphosphorylation of an industrial yeast Yarrowia lipolytica, and to a method for preparing a host system capable of producing recombinant glycoproteins free of mannosylphosphate by disruption of the gene. The mannosylphosphorylation is suppressed by the disruption of YlMPO1 gene according to the present invention, thereby achieving humanization of glycosylation pathway of Yarrowia lipolytica.
摘要翻译： 本发明涉及一种在工业酵母解脂耶氏酵母（Yarrowia lipolytica）的甘露糖基磷酸化中起重要作用的新型YlMPO1基因，以及能够通过破坏基因产生不含磷酸甘露糖基的重组糖蛋白的宿主系统的方法。 通过根据本发明的YlMPO1基因的破坏来抑制甘露糖基磷酸化，从而实现解脂耶氏酵母的糖基化途径的人源化。
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2.发明申请NOVEL HANSENULA POLYMORPHA GENE CODING FOR DOLICHYL-PHOSPHATE- MANNOSE DEPENDENT ALPHA-1,3 MANNOSYLTRANSFERASE AND PROCESS FOR THE PRODUCTION OF RECOMBINANT GLYCOPROTEINS WITH HANSENULA POLYMORPHA MUTANT STRAIN DEFICIENT IN THE SAME GENE 有权新型HANSENULA多聚异戊二烯基因编码多赖氨酸 - 曼尼斯依赖性ALPHA-1,3人造丝素和用于生产与其相同基因中的汉逊酵母多聚突变体缺陷的重组型糖蛋白的方法公开(公告)号：US20120202248A1
公开(公告)日：2012-08-09
申请号：US13451223
申请日：2012-04-19
申请人： Hyun Ah Kang , Jeong Seok Park , Moo Woong Kim , Eun Jung Kim , Hye Yun Moon , Doo Byoung Oh , Joo Hyung Heo , Sang Ki Rhee
发明人： Hyun Ah Kang , Jeong Seok Park , Moo Woong Kim , Eun Jung Kim , Hye Yun Moon , Doo Byoung Oh , Joo Hyung Heo , Sang Ki Rhee
IPC分类号： C12P21/06
CPC分类号： C12N9/1051
摘要： The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a Hansenula polymorpha system. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent α-1,3-mannosyltransferase gene from H. polymorpha, constructing a H. polymorpha mutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan.
摘要翻译： 本发明涉及通过使用汉逊酵母多形汉逊酵母系统遗传操作参与糖基化的酶来生产具有降低的糖基化的人型糖蛋白的方法。 具体地说，本发明涉及通过鉴定多形汉逊酵母多糖基 - 磷酸 - 甘露糖依赖性α-1,3-甘露糖基转移酶基因来产生人型糖蛋白的方法，构建产生糖多糖的多形汉逊酵母突变菌株 通过破坏鉴定的基因来降低糖基化，并使突变株进行各种遗传操作以合成人型聚糖。
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3.发明授权Hansenula polymorpha gene coding for dolichyl-phosphate-mannose dependent alpha-1,3-mannosyltransferase and process for the production of recombinant glycoproteins with Hansenula polymorpha mutant strain deficient in the same gene 有权编码二酰基 - 磷酸 - 甘露糖依赖性α-1,3-甘露糖基转移酶的汉逊酵母多形汉逊酵母基因以及与相同基因缺失的多形汉逊酵母突变株产生重组糖蛋白的方法公开(公告)号：US08187858B2
公开(公告)日：2012-05-29
申请号：US12091217
申请日：2006-10-26
申请人： Hyun Ah Kang , Jeong Seok Park , Moo Woong Kim , Eun Jung Kim , Hye Yun Moon , Doo Byoung Oh , Joo Hyung Heo , Sang Ki Rhee
发明人： Hyun Ah Kang , Jeong Seok Park , Moo Woong Kim , Eun Jung Kim , Hye Yun Moon , Doo Byoung Oh , Joo Hyung Heo , Sang Ki Rhee
CPC分类号： C12N9/1051
摘要： The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a Hansenula polymorpha system. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent ¥á-1,3-mannosyltransferase gene from H. polymorpha, constructing a H. polymorpha mutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan.
摘要翻译： 本发明涉及通过使用汉逊酵母多形汉逊酵母系统遗传操作参与糖基化的酶来生产具有降低的糖基化的人型糖蛋白的方法。 具体地说，本发明涉及通过鉴定多形汉逊酵母多糖基 - 磷酸 - 甘露糖依赖性的α-1,3-甘露糖基转移酶基因来产生人型糖蛋白的方法，构建产生糖蛋白的多形汉逊酵母突变菌株 通过破坏鉴定的基因表现出降低的糖基化，并使突变株进行各种遗传操作以合成人型聚糖。
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4.发明申请NOVEL GENE FROM HANCENULA POLYMORPHA CAPABLE OF CONTROLLING UNFOLDED PROTEIN RESPONSE AND METHOD FOR INCREASING EFFECT OF SECRETION USING THE SAME 有权来自可控制不相关蛋白质反应的HANCENULA多聚体的新基因和使用其分泌增加效果的方法公开(公告)号：US20100297694A1
公开(公告)日：2010-11-25
申请号：US12304354
申请日：2007-06-13
申请人： Hyun Ah Kang , Hye-Yun Moon , Doo-Byoung Oh , Sang Ki Rhee
发明人： Hyun Ah Kang , Hye-Yun Moon , Doo-Byoung Oh , Sang Ki Rhee
摘要： The present invention relates to a novel H. polymorpha gene regulating Unfolded Protein Response (UPR) and a method for improving the efficiency of secretory expression of a recombinant protein using the same, more particularly, a method for improving the efficiency of secretory expression of a recombinant protein by identifying, modifying, and optimizing the HpHAC1 gene, which encodes a key transcription regulatory factor of the UPR mechanism in H. polymorpha, and a regulatory mechanism mediated by the gene. According to the present invention, the secretory expression system of H. polymorpha can be used for the mass-production of secretory proteins for industrial and medical applications, thereby producing a large amount of the proteins at low cost. Accordingly, the method is employed in the production of the proteins for industrial and medical use, so as to reduce economic burden for a patient, and contribute to the improvement in the welfare of all mankind.
摘要翻译： 本发明涉及调节未折叠蛋白质反应（UPR）的新型多形汉逊酵母基因和提高重组蛋白的分泌表达效率的方法，更具体地说，涉及提高分泌表达效率的方法 重组蛋白通过鉴定，修饰和优化HpHAC1基因，其编码多形汉逊酵母中UPR机制的关键转录调节因子，以及由该基因介导的调节机制。 根据本发明，多形汉逊酵母的分泌表达系统可用于大规模生产用于工业和医疗应用的分泌蛋白质，从而以低成本生产大量的蛋白质。 因此，该方法被用于生产用于工业和医疗用途的蛋白质，以减少患者的经济负担，并有助于改善全人类的福利。
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公开(公告)号：US08236520B2
公开(公告)日：2012-08-07
申请号：US12952116
申请日：2010-11-22
申请人： Hyun Ah Kang , Jae Kap Jeong , Ohsuk Kwon , Doo-Byoung Oh , Seonghun Kim
发明人： Hyun Ah Kang , Jae Kap Jeong , Ohsuk Kwon , Doo-Byoung Oh , Seonghun Kim
CPC分类号： C12Y302/01023 , A61K38/00 , C12N9/2471
摘要： The present invention relates to beta-galactosidase derived from Streptococcus pneumoniae, a BgaC protein exhibiting the enzyme activity, and a method for using the same. The protein can be used in the modification and analysis of sugar chain and used as an anti-cancer agent.
摘要翻译： 本发明涉及来源于肺炎链球菌的β-半乳糖苷酶，表现出酶活性的BgaC蛋白质及其使用方法。 该蛋白质可用于糖链的修饰和分析，并用作抗癌剂。
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6.发明申请Novel YLMPO1 Gene Derived From Yarrowia Lipolytica and A Process for Preparing a Glycoprotein Not Being Mannosylphosphorylated By Using a Mutated Yarrowia Lipolytica in Which YLMPO1 Gene is Disrupted 有权来自解脂耶氏酵母的新型YLMPO1基因和通过使用其中YLMPO1基因被破坏的突变型耶氏酵母属（Larrowia Lipolytica）而不是正羰基磷酸化的糖蛋白的制备方法公开(公告)号：US20100227362A1
公开(公告)日：2010-09-09
申请号：US12598887
申请日：2007-11-30
申请人： Jeong-Nam Park , Yunkyoung Song , Jeong-Yoon Kim , Doo-Byoung Oh , Hyun Ah Kang
发明人： Jeong-Nam Park , Yunkyoung Song , Jeong-Yoon Kim , Doo-Byoung Oh , Hyun Ah Kang
CPC分类号： C12N15/815 , C12N9/1081
摘要： The present invention relates to a novel YlMPOl gene which plays an important role in mannosylphosphorylation of an industrial yeast Yarrowia lipolytica, and to a method for preparing a host system capable of producing recombinant glycoproteins free of mannosylphosphate by disruption of the gene. The mannosylphosphorylation is suppressed by the disruption of YlMPOl gene according to the present invention, thereby achieving humanization of glycosylation pathway of Yarrowia lipolytica.
摘要翻译： 本发明涉及在工业酵母解脂耶氏酵母解脂耶氏酵母的甘油糖基磷酸化中起重要作用的新型YlMPO1基因，以及能够通过破坏基因产生不含磷酸甘露糖基的重组糖蛋白的宿主系统的方法。 通过根据本发明的YlMPO1基因的破坏来抑制甘露糖基磷酸化，从而实现解脂耶氏酵母的糖基化途径的人源化。
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公开(公告)号：US20080248019A1
公开(公告)日：2008-10-09
申请号：US11823333
申请日：2007-06-25
申请人： Hyun Ah Kang , Jae Kap Jeong , Ohsuk Kwon , Doo-Byoung Oh , Seonghun Kim
发明人： Hyun Ah Kang , Jae Kap Jeong , Ohsuk Kwon , Doo-Byoung Oh , Seonghun Kim
CPC分类号： C12Y302/01023 , A61K38/00 , C12N9/2471
摘要： The present invention relates to a novel beta-galactosidase derived from Streptococcus pneumoniae, a BgaC protein exhibiting the enzyme activity, and a method for using the same. The protein can be used in the modification and analysis of sugar chain and used as an anti-cancer agent.
摘要翻译： 本发明涉及来自肺炎链球菌的新型β-半乳糖苷酶，表现出酶活性的BgaC蛋白质及其使用方法。 该蛋白质可用于糖链的修饰和分析，并用作抗癌剂。
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8.发明授权Process for producing recombinant glycoproteins by culturing a Hansenula polymorpha mutant strain 有权通过培养汉逊酵母多形汉逊酵母突变菌株来生产重组糖蛋白的方法公开(公告)号：US08685671B2
公开(公告)日：2014-04-01
申请号：US13451223
申请日：2012-04-19
申请人： Hyun Ah Kang , Jeong Seok Park , Moo Woong Kim , Eun Jung Kim , Hye Yun Moon , Doo Byoung Oh , Joo Hyung Heo , Sang Ki Rhee
发明人： Hyun Ah Kang , Jeong Seok Park , Moo Woong Kim , Eun Jung Kim , Hye Yun Moon , Doo Byoung Oh , Joo Hyung Heo , Sang Ki Rhee
CPC分类号： C12N9/1051
摘要： The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a Hansenula polymorpha system. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent α-1,3-mannosyltransferase gene from H. polymorpha, constructing a H. polymorpha mutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan.
摘要翻译： 本发明涉及通过使用汉逊酵母多形汉逊酵母系统遗传操作参与糖基化的酶来生产具有降低的糖基化的人型糖蛋白的方法。 具体地说，本发明涉及通过鉴定多形汉逊酵母多糖基 - 磷酸 - 甘露糖依赖性α-1,3-甘露糖基转移酶基因来产生人型糖蛋白的方法，构建产生糖多糖的多形汉逊酵母突变菌株 通过破坏鉴定的基因来降低糖基化，并使突变株进行各种遗传操作以合成人型聚糖。
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9.发明申请RECOMBINANT BETA-GALACTOSIDASE DERIVED FROM STREPTOCOCCUS PNEUMONIAE 有权来自STREPTOCOCCUS PNEUMONIAE的重组β-GALACTOSIDASE公开(公告)号：US20110287463A1
公开(公告)日：2011-11-24
申请号：US12952116
申请日：2010-11-22
申请人： Hyun Ah Kang , Jae Kap Jeong , Ohsuk Kwon , Doo-Byoung Oh , Seonghun Kim
发明人： Hyun Ah Kang , Jae Kap Jeong , Ohsuk Kwon , Doo-Byoung Oh , Seonghun Kim
IPC分类号： C12Q1/34
CPC分类号： C12Y302/01023 , A61K38/00 , C12N9/2471
摘要： The present invention relates to a novel beta-galactosidase derived from Streptococcus pneumoniae, a BgaC protein exhibiting the enzyme activity, and a method for using the same. The protein can be used in the modification and analysis of sugar chain and used as an anti-cancer agent.
摘要翻译： 本发明涉及来自肺炎链球菌的新型β-半乳糖苷酶，表现出酶活性的BgaC蛋白质及其使用方法。 该蛋白质可用于糖链的修饰和分析，并用作抗癌剂。
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10.发明授权Gene from Hancenula polymorpha capable of controlling unfolded protein response and method for increasing effect of secretion using the same 有权能够控制未折叠蛋白质反应的Hancenula polymorpha的基因和使用其分泌增加效果的方法公开(公告)号：US08323926B2
公开(公告)日：2012-12-04
申请号：US12304354
申请日：2007-06-13
申请人： Hyun Ah Kang , Hye-Yun Moon , Doo-Byoung Oh , Sang Ki Rhee
发明人： Hyun Ah Kang , Hye-Yun Moon , Doo-Byoung Oh , Sang Ki Rhee
摘要： The present invention relates to a novel H. polymorpha gene regulating Unfolded Protein Response (UPR) and a method for improving the efficiency of secretory expression of a recombinant protein using the same, more particularly, a method for improving the efficiency of secretory expression of a recombinant protein by identifying, modifying, and optimizing the HpHAC1 gene, which encodes a key transcription regulatory factor of the UPR mechanism in H. polymorpha, and a regulatory mechanism mediated by the gene. According to the present invention, the secretory expression system of H. polymorpha can be used for the mass-production of secretory proteins for industrial and medical applications, thereby producing a large amount of the proteins at low cost. Accordingly, the method is employed in the production of the proteins for industrial and medical use, so as to reduce economic burden for a patient, and contribute to the improvement in the welfare of all mankind.
摘要翻译： 本发明涉及调节未折叠蛋白质反应（UPR）的新型多形汉逊酵母基因和提高重组蛋白的分泌表达效率的方法，更具体地说，涉及提高分泌表达效率的方法 重组蛋白通过鉴定，修饰和优化HpHAC1基因，其编码多形汉逊酵母中UPR机制的关键转录调节因子，以及由该基因介导的调节机制。 根据本发明，多形汉逊酵母的分泌表达系统可用于大规模生产用于工业和医疗应用的分泌蛋白质，从而以低成本生产大量的蛋白质。 因此，该方法被用于生产用于工业和医疗用途的蛋白质，以减少患者的经济负担，并有助于改善全人类的福利。
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