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公开(公告)号：US20220065879A1
公开(公告)日：2022-03-03
申请号：US17009225
申请日：2020-09-01
Inventor： Zhaoping LIU , Stephen BARNES , Dominique PEREZ , Christopher SUSKI
Abstract: A method for automatic sampling of immune cells from a biological fluid sample, e.g., whole blood, deposited in a well of a microwell plate. The sample contains red blood cells (RBCs) and magnetic beads which are designed to bind to the RBCs. The microwell plate is placed on a shaker having a magnetic adapter including at least one magnet. The magnet causes the RBCs bound to the magnetic beads to be attracted to and migrate to a wall of the well (e.g., the bottom or side wall) and be held against the wall. The shaker is then operated to shake the microwell plate in a manner and for a time period so as to suspend substantially evenly or homogeneously the immune cells in the biological fluid sample within a region of the well but still retain the holding of the RBCs to the wall of the well such that the immune cells are substantially isolated from the RBCs in the region of the well. During or after shaking, a sample probe is then lowered into the region of the well to withdraw a portion of the sample containing the immune cells.
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公开(公告)号：US11169073B2
公开(公告)日：2021-11-09
申请号：US16422814
申请日：2019-05-24
Applicant: Essen Instruments, Inc.
Inventor： Stephen Barnes , Zachary Kane , Richard Bennett , Jason Barrett
Abstract: The disclosure provides example rinse station apparatus, cartridges, and methods for flow cytometry. The rinse station apparatus includes: (a) a cartridge docking station having a base with a recessed receptacle for a cartridge, a vertical support coupled to the base's first end and a top support coupled to the vertical support and cantilevered over the base, the top support has an opening that aligns with the cartridge's opening, (b) a locking arm coupled to the base's second end, the locking arm's free end has a ridge to cooperate with a detent coupled to the cartridge's rear wall to retain the cartridge in place, (c) a spring coupled to the vertical support's front face to apply force to the cartridge's front wall to bias the cartridge toward the locking arm, and (d) a load cell coupled to the base of the cartridge docking station, the load cell measure's the cartridge's weight.
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公开(公告)号：US20200249163A1
公开(公告)日：2020-08-06
申请号：US16264819
申请日：2019-02-01
Inventor： Paul Joseph Bierdz , Nevine Holtz , Eric William Endsley
Abstract: Systems and methods are provided for microscopically and fluorescently imaging cell-bearing biological samples or other samples of interest. A microscope objective or other optical elements that exhibits chromatic aberration can be used to obtain images of fluorophores or other contrast agents at different wavelengths. The obtained images are then used to correct each other, e.g., to remove artifacts in an image of a shorter-wavelength fluorophore that are caused by cross-talk from a longer-wavelength fluorophore. A longer-wavelength image, taken at a focal distance corresponding to the shorter-wavelength fluorophore, is taken and used to subtract the activity of the longer-wavelength fluorophore in the shorter-wavelength image. The longer-wavelength image may be taken using a microscope set to the shorter-wavelength focal distance. Alternatively, the longer-wavelength image may be simulated by applying a blurring filter or other methods to a longer-wavelength image taken when the microscope is set to the longer-wavelength focal distance.
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公开(公告)号：US10724956B1
公开(公告)日：2020-07-28
申请号：US16264819
申请日：2019-02-01
Applicant: Essen Instruments, Inc.
Inventor： Paul Joseph Bierdz , Nevine Holtz , Eric William Endsley
Abstract: Systems and methods are provided for microscopically and fluorescently imaging cell-bearing biological samples or other samples of interest. A microscope objective or other optical elements that exhibits chromatic aberration can be used to obtain images of fluorophores or other contrast agents at different wavelengths. The obtained images are then used to correct each other, e.g., to remove artifacts in an image of a shorter-wavelength fluorophore that are caused by cross-talk from a longer-wavelength fluorophore. A longer-wavelength image, taken at a focal distance corresponding to the shorter-wavelength fluorophore, is taken and used to subtract the activity of the longer-wavelength fluorophore in the shorter-wavelength image. The longer-wavelength image may be taken using a microscope set to the shorter-wavelength focal distance. Alternatively, the longer-wavelength image may be simulated by applying a blurring filter or other methods to a longer-wavelength image taken when the microscope is set to the longer-wavelength focal distance.
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公开(公告)号：US20200217776A1
公开(公告)日：2020-07-09
申请号：US16781382
申请日：2020-02-04
Abstract: A method for evaluating a biological material for unassociated virus-size particles having a particular epitope uses a fluorescent antibody stain specific for binding with the epitope and a fluid sample with the virus-size particles and fluorescent antibody stain is subjected to flow cytometry with identification of fluorescent emission detection events indicative of passage through a flow cell of a flow cytometer of unassociated labeled particles of virus size including such a virus-size particle and fluorescent antibody stain.
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Patent Agency Ranking
公开(公告)号：US10539497B2
公开(公告)日：2020-01-21
申请号：US15315698
申请日：2015-06-02
Applicant: Essen Instruments, Inc.
Inventor： Andrew W. Smolak , Erica Dawson Tenent , Kathy L. Rowlen , Garrett S. Wilson , Christopher H. Converse , Evan M. Toth
Abstract: A method and a flow cytometer system in which a light path is aligned to optimally direct light from a light source to a flow cell of a flow cytometer instrument are provided. The flow cytometer may include an orientable mirror disposed in the light path between the light source and the flow cell. By changing an orientation of the mirror through a sequence of different orientations, an optimal orientation of the mirror may be determined, and the mirror may be oriented accordingly before proceeding to conduct a flow cytometry investigation.
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7.发明授权公开(公告)号：US10416067B2
公开(公告)日：2019-09-17
申请号：US15616168
申请日：2017-06-07
Applicant: Essen Instruments, Inc.
Inventor： Aaron Bryce Kennington
Abstract: A method for detecting a separation gas in a fluid flow stream is provided herein. In one example, a voltage output signal is generated by a scatter detector of a flow cytometer as a flow stream of a plurality of gas-separated samples passes through the flow cytometer. The voltage output signal is sampled, and a timestamp and a voltage value are recorded for each sampled voltage of the voltage output signal that is greater than a separation gap threshold. In some examples the separation gap threshold is at least two times greater than a maximum voltage output of the samples. Flow cytometry systems including software configured to perform these method steps are also described.
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公开(公告)号：US11149242B2
公开(公告)日：2021-10-19
申请号：US16827609
申请日：2020-03-23
Inventor： Kirk S. Schroeder , Bradley D. Neagle , Eric Endsley , Daniel Appledorn , Keith Morton
Abstract: Systems, methods, and apparatuses of controlling fluid flow are disclosed. An apparatus includes a first microplate having a first open portion and defining one or more first wells therein, a second microplate having a second open portion and defining one or more second wells therein, and a pneumatic lid constructed of styrene ethylene butylene styrene (SEBS). The pneumatic lid extends over the first open portion and the second open portion and includes one or more microfluidic channels that fluidly couple the one or more first wells to the one or more second wells. The pneumatic lid provides an airtight seal over the first microplate and the second microplate.
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公开(公告)号：US11137337B2
公开(公告)日：2021-10-05
申请号：US16253060
申请日：2019-01-21
Applicant: Essen Instruments, Inc.
Inventor： Tyler Gates , Jeffrey Steaffens
Abstract: Flow cytometry investigation and flow cytometry result analysis to determine an optimized dilution factor range for flow cytometry investigation of a target sample fluid stock using a flow cytometer. The optimized dilution factor range may be determined using a screening assay module of a flow cytometry system to analyze flow cytometry results determined to fall within a dynamic range of a flow cytometer to determine the optimized dilution factor range for a given flow cytometer to investigate a given target sample fluid stock. In turn, a titer assay module may be executed to prepare particle titer results based on optimized target fluid samples provided within the optimized dilution factor range. The screening assay module and/or the titer assay module may provide automated data processing with data notifications and confirmations to provide robust data analysis.
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10.发明申请公开(公告)号：US20210270829A1
公开(公告)日：2021-09-02
申请号：US17255012
申请日：2019-08-28
Inventor： Zhaoping LIU
IPC: G01N33/543
Abstract: Disclosed herein are methods for determining a concentration of at least one low concentration protein and at least one high concentration protein in a biological sample.
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