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    Hydrazine derivatized cells
    3.
    发明授权
    Hydrazine derivatized cells 失效
    肼衍生细胞

    公开(公告)号：US5637472A

    公开(公告)日：1997-06-10

    申请号：US391869

    申请日：1995-02-22

    申请人： Edward N. Granados ,  Daniel F. Heiman ,  Herbert S. Chow ,  Maria L. Thaler

    发明人： Edward N. Granados ,  Daniel F. Heiman ,  Herbert S. Chow ,  Maria L. Thaler

    IPC分类号： C12N5/06 ,  G01N33/50 ,  G01N33/543 ,  G01N33/554 ,  G01N33/556 ,  G01N33/569

    CPC分类号： G01N33/554 ,  G01N33/5005 ,  G01N33/54313 ,  G01N33/556 ,  G01N33/56977 ,  Y10T436/101666

    摘要： A method is provided for the preparation of semisolid particles or cells that have been chemically derivatized resulting in hydrazide functionalities covalently incorporated onto cell surface membrane molecules of the semisolid particles or cells, as well as a method for determining the levels of hydrazide groups on the particles or cells. The method involves the preparation and analysis of red blood cells chemically derivatized in such a manner that hydrazide functionalities have been covalently incorporated onto the cell membrane molecules, the preparation of hydrazine derivatized cells and the use thereof of the novel cells in agglutination reactions to promote the attachment of antigens and antibodies to the hydrazine derivatized cells without loss of antibody activity or the blocking of the etitope of interest.

    摘要翻译： 提供了一种制备半固体颗粒或细胞的方法，该半固体颗粒或细胞被化学衍生化，导致共价掺入到半固体颗粒或细胞的细胞表面膜分子上的酰肼官能团，以及确定颗粒上酰肼基团水平的方法 或细胞。 该方法涉及红细胞的制备和分析，其方法是将酰肼官能团共价掺入到细胞膜分子上，制备肼衍生化细胞，以及在凝集反应中使用新细胞促进 将抗原和抗体连接到肼衍生化的细胞上，而不会损失抗体活性或阻断目的蛋白酶。

    Nucleic acid primers and probes for detecting tumor cells
    5.
    发明授权
    Nucleic acid primers and probes for detecting tumor cells 有权
    用于检测肿瘤细胞的核酸引物和探针

    公开(公告)号：US06203992B1

    公开(公告)日：2001-03-20

    申请号：US09419212

    申请日：1999-10-15

    申请人： Edward N. Granados ,  Christi Powell Scheffel

    发明人： Edward N. Granados ,  Christi Powell Scheffel

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6886 ,  C12Q2600/16

    摘要： Nucleic acid sequences are provided that are useful as amplification primers and hybridization probes for detecting CK19 target sequence in a test sample. The primers and probes can be employed in amplification based methods for detecting the presence of CK19 sequences in a test sample. The amplification product can be detected with the assistance of microparticle capture techniques.

    摘要翻译： 提供了可用作检测样品中CK19靶序列的扩增引物和杂交探针的核酸序列。 引物和探针可用于基于扩增的检测样品中CK19序列存在的方法。 可以借助微粒捕获技术检测扩增产物。

    Method for quantitating hydrazide groups
    6.
    发明授权
    Method for quantitating hydrazide groups 失效
    定量酰肼基团的方法

    公开(公告)号：US5591598A

    公开(公告)日：1997-01-07

    申请号：US391876

    申请日：1995-02-22

    申请人： Edward N. Granados ,  Daniel F. Heiman ,  Herbert S. Chow ,  Maria L. Thaler

    发明人： Edward N. Granados ,  Daniel F. Heiman ,  Herbert S. Chow ,  Maria L. Thaler

    IPC分类号： C12N5/06 ,  G01N33/50 ,  G01N33/543 ,  G01N33/554 ,  G01N33/556 ,  G01N33/569 ,  C12Q1/02 ,  C12Q1/24

    CPC分类号： G01N33/554 ,  G01N33/5005 ,  G01N33/54313 ,  G01N33/556 ,  G01N33/56977 ,  Y10T436/101666

    摘要： A method is provided for the preparation of semisolid particles or cells that have been chemically derivatized resulting in hydrazide functionalities covalently incorporated onto cell surface membrane molecules of the semisolid particles or cells, as well as a method for determining the levels of hydrazide groups on the particles or cells. The method involves the preparation and analysis of red blood cells chemically derivatized in such a manner that hydrazide functionalities have been covalently incorporated onto the cell membrane molecules, the preparation of hydrazine derivatized cells and the use of the novel cells in agglutination reactions to promote the attachment of antigens and antibodies to the hydrazine derivatized cells without loss of antibody activity or the blocking of the epitope of interest.

    摘要翻译： 提供了一种制备半固体颗粒或细胞的方法，该半固体颗粒或细胞被化学衍生化，导致共价掺入到半固体颗粒或细胞的细胞表面膜分子上的酰肼官能团，以及确定颗粒上酰肼基团水平的方法 或细胞。 该方法包括以这样一种方式进行化学衍生的红细胞的制备和分析，即将酰肼官能团共价掺入到细胞膜分子上，肼衍生化细胞的制备以及新型细胞在凝集反应中的使用以促进附着 的抗原和针对肼衍生化细胞的抗体，而不损失抗体活性或阻断感兴趣的表位。