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    FOUR-GENE PATHWAY FOR WAX ESTER SYNTHESIS
    3.
    发明申请
    FOUR-GENE PATHWAY FOR WAX ESTER SYNTHESIS 有权
    四基因路由合成

    公开(公告)号：US20130224811A1

    公开(公告)日：2013-08-29

    申请号：US13408270

    申请日：2012-02-29

    申请人： Erik Holtzapple ,  John H. Verruto

    发明人： Erik Holtzapple ,  John H. Verruto

    IPC分类号： C12P7/64 ,  C12N1/13 ,  C12N15/55 ,  C12N15/63

    CPC分类号： C12P7/6436 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/16

    摘要： The invention relates to methods for producing a wax ester in recombinant host cells engineered to express a thioesterase, an acyl-CoA synthetase, an alcohol-forming fatty acyl reductase, and a wax ester synthase. The methods of the invention may take place in photosynthetic microorganisms, and particularly in cyanobacteria. Isolated nucleotide molecules and vectors expressing the thioesterase, acyl-CoA synthetase, alcohol-forming fatty acyl reductase, and wax ester synthase, recombinant host cells expressing the thioesterase, acyl-CoA synthetase, alcohol-forming fatty acyl reductase, and wax ester synthase, and systems for producing a wax ester via a pathway using these four enzymes, are also provided.

    摘要翻译： 本发明涉及在工程化以表达硫酯酶，酰基辅酶A合成酶，成醇脂肪酰基还原酶和蜡酯合成酶的重组宿主细胞中生产蜡酯的方法。 本发明的方法可以在光合微生物中发生，特别是在蓝细菌中。 表达硫酯酶，酰基辅酶A合成酶，成醇脂肪酰基还原酶和蜡酯合成酶的分离的核苷酸分子和载体，表达硫酯酶，酰基辅酶A合成酶，成醇脂肪酰基还原酶和蜡酯合成酶的重组宿主细胞， 还提供了通过使用这四种酶生产蜡酯的途径。

    ACYL-ACP WAX ESTER SYNTHASES
    4.
    发明申请
    ACYL-ACP WAX ESTER SYNTHASES 有权

    公开(公告)号：US20130078684A1

    公开(公告)日：2013-03-28

    申请号：US13413426

    申请日：2012-03-06

    申请人： Erik Holtzapple ,  John H. Verruto

    发明人： Erik Holtzapple ,  John H. Verruto

    IPC分类号： C12N1/21 ,  C12P7/64

    CPC分类号： C12P7/6409 ,  C07K14/21 ,  C07K14/28 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/88 ,  C12N15/70 ,  C12N15/74 ,  C12P5/02 ,  C12P7/04 ,  C12P7/6436 ,  C12Y102/0108 ,  C12Y203/0102 ,  C12Y203/01075 ,  C12Y203/01084 ,  C12Y301/0202 ,  C12Y401/01 ,  C12Y401/99005

    摘要： The invention relates to acyl-CoA-independent methods of producing a wax ester in recombinant host cells engineered to express an acyl-ACP wax ester synthase, and an alcohol-forming acyl-ACP reductase. The methods of the invention may take place in photosynthetic microorganisms, and particularly in cyanobacteria. Isolated nucleotide molecules and vectors expressing an acyl-ACP wax ester synthase and/or an alcohol-forming acyl-ACP reductase, recombinant host cells expressing an acyl-ACP wax ester synthase and optionally an alcohol-forming acyl-ACP reductase, and systems for producing a wax ester via an acyl-CoA-independent pathway, are also provided.

    摘要翻译： 本发明涉及在工程化表达酰基-ACP蜡酯合酶的重组宿主细胞中产生蜡酯的酰基辅酶A独立方法和形成醇的酰基-ACP还原酶。 本发明的方法可以在光合微生物中发生，特别是在蓝细菌中。 分离的核苷酸分子和表达酰基-ACP蜡酯合酶和/或成醇酰基-ACP还原酶的载体，表达酰基-ACP蜡酯合成酶和任选的形成醇的酰基-ACP还原酶的重组宿主细胞，以及用于 还提供了通过酰基辅酶A独立途径产生蜡酯。

    Four-gene pathway for wax ester synthesis
    5.
    发明授权
    Four-gene pathway for wax ester synthesis 有权
    蜡酯合成的四基因途径

    公开(公告)号：US08962299B2

    公开(公告)日：2015-02-24

    申请号：US13408270

    申请日：2012-02-29

    申请人： Erik Holtzapple ,  John H. Verruto

    发明人： Erik Holtzapple ,  John H. Verruto

    IPC分类号： C12N1/12 ,  C12N15/00 ,  C12P7/64

    CPC分类号： C12P7/6436 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/16

    摘要： The invention relates to methods for producing a wax ester in recombinant host cells engineered to express a thioesterase, an acyl-CoA synthetase, an alcohol-forming fatty acyl reductase, and a wax ester synthase. The methods of the invention may take place in photosynthetic microorganisms, and particularly in cyanobacteria. Isolated nucleotide molecules and vectors expressing the thioesterase, acyl-CoA synthetase, alcohol-forming fatty acyl reductase, and wax ester synthase, recombinant host cells expressing the thioesterase, acyl-CoA synthetase, alcohol-forming fatty acyl reductase, and wax ester synthase, and systems for producing a wax ester via a pathway using these four enzymes, are also provided.

    摘要翻译： 本发明涉及在工程化以表达硫酯酶，酰基辅酶A合成酶，成醇脂肪酰基还原酶和蜡酯合成酶的重组宿主细胞中生产蜡酯的方法。 本发明的方法可以在光合微生物中发生，特别是在蓝细菌中。 表达硫酯酶，酰基辅酶A合成酶，成醇脂肪酰基还原酶和蜡酯合成酶的分离的核苷酸分子和载体，表达硫酯酶，酰基辅酶A合成酶，成醇脂肪酰基还原酶和蜡酯合成酶的重组宿主细胞， 还提供了通过使用这四种酶生产蜡酯的途径。