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公开(公告)号：US20100132067A1
公开(公告)日：2010-05-27
申请号：US12571471
申请日：2009-10-01
Applicant: MICHAEL GODWIN , FENG HAN , ALEC A. HAYES , XU HU , SOON-CHUN JEONG , GUIHUA LU , M.A. SAGHAI MAROOF
Inventor： MICHAEL GODWIN , FENG HAN , ALEC A. HAYES , XU HU , SOON-CHUN JEONG , GUIHUA LU , M.A. SAGHAI MAROOF
CPC classification number: A01H1/04 , C07K14/415 , C12N15/8282 , C12N15/8283 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/172
Abstract: The invention provides novel molecular genetic markers in soybean, where the markers are useful, for example, in the marker-assisted selection of gene alleles that impart disease-resistance, thereby allowing the identification and selection of a disease-resistant plant. The markers also find use in positional cloning of disease-resistance genes.
Abstract translation: 本发明在大豆中提供了新的分子遗传标记，其中标记可用于例如赋予抗病性的基因等位基因的标记辅助选择，从而允许鉴定和选择抗病植物。 标记也用于抗病基因的定位克隆。
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2.发明授权公开(公告)号：US07504558B2
公开(公告)日：2009-03-17
申请号：US11556211
申请日：2006-11-03
CPC classification number: C07K14/415 , C12N15/8227 , C12N15/8239 , C12N15/8285 , Y02A40/164
Abstract: The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred and inducible promoter for the gene encoding a soybean ripening-related protein. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
Abstract translation: 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于编码大豆成熟相关蛋白的基因的根优选和诱导型启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将可操作地连接到本发明的根优选启动子的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。
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公开(公告)号：US06709865B1
公开(公告)日：2004-03-23
申请号：US09714767
申请日：2000-11-16
Applicant: Dennis L. Bidney , Jonathan P. Duvick , Carol Hendrick , Xu Hu , Guihua Lu , Oswald R. Crasta
Inventor： Dennis L. Bidney , Jonathan P. Duvick , Carol Hendrick , Xu Hu , Guihua Lu , Oswald R. Crasta
IPC: C12N500
CPC classification number: C12N9/0073 , C07K14/415 , C12N9/0006 , C12N15/8216 , C12N15/8237 , C12N15/8239 , C12N15/8261 , C12N15/8282 , Y02A40/146
Abstract: Methods and compositions for modulating development and defense response are provided. Nucleotide sequences encoding a LOX protein are provided. Nucleotide sequences comprising the LOX promoter are also provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and the plant defense response. Transformed plants, plant cells, tissues, and seed are also provided.
Abstract translation: 提供了调节发育和防御反应的方法和组合物。 提供了编码LOX蛋白的核苷酸序列。 还提供了包含LOX启动子的核苷酸序列。 序列可用于表达盒中用于调节发育，发育途径和植物防御反应。 还提供了转化的植物，植物细胞，组织和种子。
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公开(公告)号：US08999712B1
公开(公告)日：2015-04-07
申请号：US12942549
申请日：2010-11-09
Applicant: Guihua Lu , Julie Qi , Jennifer S. Tallman , Xu Hu , Yaxin Li
Inventor： Guihua Lu , Julie Qi , Jennifer S. Tallman , Xu Hu , Yaxin Li
CPC classification number: C12Q1/025
Abstract: A method for improving the sensitivity of an assay to determine the pathogenicity of a plant root pathogen using a soil amendment is presented. The method involves growing the plant root in the presence of a soil amendment after exposure of the plant root to the pathogen. A method of breeding plants is also provided.
Abstract translation: 提出了一种提高测定灵敏度以确定植物根系病原体使用土壤修复的致病力的方法。 该方法涉及在将植物根部暴露于病原体之后，在土壤修饰物的存在下生长植物根。 还提供了育种植物的方法。
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公开(公告)号：US07196247B2
公开(公告)日：2007-03-27
申请号：US10104706
申请日：2002-03-22
Applicant: Joan T. Odell , Xiaodan Yu , Xu Hu , Guihua Lu
Inventor： Joan T. Odell , Xiaodan Yu , Xu Hu , Guihua Lu
CPC classification number: C12N15/8227 , C12N15/8238 , C12N15/8239
Abstract: This invention relates to an isolated isoflavone synthase 1 (IFS1) promoter nucleic acid fragment. The invention also relates to the construction of chimeric genes comprising all or a portion of the IFS1 promoter directing the expression of transgenes, in sense or antisense orientation.
Abstract translation: 本发明涉及分离的异黄酮合成酶1（IFS1）启动子核酸片段。 本发明还涉及包含有义或反义方向指导转基因表达的全部或部分IFS1启动子的嵌合基因的构建。
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Patent Agency Ranking
公开(公告)号：US20060041955A1
公开(公告)日：2006-02-23
申请号：US11208038
申请日：2005-08-19
Applicant: Michael Godwin , Feng Han , Alec Hayes , Xu Hu , Soon-Chun Jeong , Guihua Lu , M. A. Maroof
Inventor： Michael Godwin , Feng Han , Alec Hayes , Xu Hu , Soon-Chun Jeong , Guihua Lu , M. A. Maroof
CPC classification number: A01H1/04 , C07K14/415 , C12N15/8282 , C12N15/8283 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/172
Abstract: The invention provides novel molecular genetic markers in soybean, where the markers are useful, for example, in the marker-assisted selection of gene alleles that impart disease-resistance, thereby allowing the identification and selection of a disease-resistant plant. The markers also find use in positional cloning of disease-resistance genes.
Abstract translation: 本发明在大豆中提供了新的分子遗传标记，其中标记可用于例如赋予抗病性的基因等位基因的标记辅助选择，从而允许鉴定和选择抗病植物。 标记也用于抗病基因的定位克隆。
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公开(公告)号：US06630615B1
公开(公告)日：2003-10-07
申请号：US09640419
申请日：2000-08-17
Applicant: Dennis L. Bidney , Oswald R. Crasta , Xu Hu , Guihua Lu
Inventor： Dennis L. Bidney , Oswald R. Crasta , Xu Hu , Guihua Lu
IPC: C12N1509
CPC classification number: C12N15/8216 , C07K14/415 , C12N9/00 , C12N15/8222 , C12N15/8226 , C12N15/8238 , C12N15/8239 , C12N15/8279 , C12N15/8282 , C12N15/8286 , Y02A40/162
Abstract: The invention relates to the genetic manipulation of plants, particularly to the expression of nucleotide sequences from defense-related signaling genes. Isolated nucleotide sequences encoding a neoxanthin cleavage enzyme, an amino acid permease, and a novel protein are provided. Also provided are isolated nucleotide sequences comprising promoters that drive expression in a plant in an inducible or tissue-preferred manner. The nucleotide sequences find use in increasing the resistance of plants to pathogens and other stresses, modifying ABA metabolism in plants, modifying amino acid transport and content in plants, and regulating gene expression in plants. Additionally provided are isolated proteins, and transformed plants and seeds thereof.
Abstract translation: 本发明涉及植物的遗传操作，特别涉及来自防御相关信号基因的核苷酸序列的表达。 提供了编码新黄素裂解酶，氨基酸通透酶和新型蛋白质的分离的核苷酸序列。 还提供了分离的核苷酸序列，其包含以诱导型或组织优选方式驱动植物表达的启动子。 核苷酸序列用于增加植物对病原体和其他胁迫的抗性，改变植物中的ABA代谢，修饰植物中的氨基酸转运和内容，以及调节植物中的基因表达。 另外提供了分离的蛋白质，以及转化的植物和种子。
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公开(公告)号：US06608240B1
公开(公告)日：2003-08-19
申请号：US09602472
申请日：2000-06-23
Applicant: Dennis L. Bidney , Xu Hu , Guihua Lu
Inventor： Dennis L. Bidney , Xu Hu , Guihua Lu
IPC: A01H500
CPC classification number: C07K14/415
Abstract: Compositions and methods for enhancing disease resistance in plants are provided. Compositions comprise sunflower resistance gene analogs. The methods involve transforming a plant with a resistance nucleotide sequence. The methods find use in enhancing broad-based resistance in plants to pathogens. Also provided are transformed plants, plant cells, tissues, and seeds having enhanced disease resistance. The R gene analogs can be used as molecular markers for disease resistance.
Abstract translation: 提供了增强植物抗病性的组合物和方法。 组合物包括向日葵抗性基因类似物。 该方法包括用抗性核苷酸序列转化植物。 该方法可用于增强植物对病原体的广泛抗性。 还提供了具有增强的抗病性的转化植物，植物细胞，组织和种子。 R基因类似物可用作抗病性的分子标记。
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公开(公告)号：US6140080A
公开(公告)日：2000-10-31
申请号：US150805
申请日：1998-09-10
Applicant: Wesley Bruce , Guihua Lu
Inventor： Wesley Bruce , Guihua Lu
CPC classification number: C12N15/1086 , C12N15/8222 , C12N15/8227 , C12N15/8286 , C12Q1/6811 , C12Q1/6895 , C12Q1/6897 , C12Q2600/158
Abstract: This invention relates generally to mechanisms of gene expression in plants and more specifically to regulation of expression of genes in plants in a "tissue-preferred" manner. A method for isolation of transcriptional regulatory elements that contribute to tissue-preferred gene expression is disclosed. Transcriptional regulatory elements isolated using the methods of this invention are demonstrated to direct tissue-preferred gene expression of genes within certain tissues of a plant. DNA molecules representing tissue-preferred transcriptional regulatory elements, vectors comprising said DNA molecules and transgenic plants comprising said vectors are demonstrated. These transcriptional regulatory units are utilized to drive tissue-preferred expression of a gene that confers a selective advantage upon a plant.
Abstract translation: 本发明一般涉及植物中基因表达的机制，更具体地涉及以“组织优选”方式调控植物中基因表达。 公开了一种用于分离有助于组织优选基因表达的转录调控元件的方法。 证明使用本发明的方法分离的转录调节元件可以指导植物某些组织内基因的组织优选基因表达。 证明代表组织优选的转录调节元件的DNA分子，包含所述DNA分子的载体和包含所述载体的转基因植物。 这些转录调控单元用于驱动赋予植物选择性优势的基因的组织优选表达。
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10.发明申请公开(公告)号：US20070192897A1
公开(公告)日：2007-08-16
申请号：US11556211
申请日：2006-11-03
CPC classification number: C07K14/415 , C12N15/8227 , C12N15/8239 , C12N15/8285 , Y02A40/164
Abstract: The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred and inducible promoter for the gene encoding a soybean ripening-related protein. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
Abstract translation: 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于编码大豆成熟相关蛋白的基因的根优选和诱导型启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将可操作地连接到本发明的根优选启动子的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。
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