公开(公告)号：US06454945B1

公开(公告)日：2002-09-24

申请号：US09703764

申请日：2000-11-01

申请人： Bernhard H. Weigl ,  Paul Yager ,  James P. Brody ,  Mark R. Holl ,  Fred K. Forster ,  Eric Altendorf ,  Paul C. Galambos ,  Margaret Kenny ,  David Schutte ,  Gregory Hixson ,  Diane Zebert ,  Andrew Kamholz ,  Caicai Wu

发明人： Bernhard H. Weigl ,  Paul Yager ,  James P. Brody ,  Mark R. Holl ,  Fred K. Forster ,  Eric Altendorf ,  Paul C. Galambos ,  Margaret Kenny ,  David Schutte ,  Gregory Hixson ,  Diane Zebert ,  Andrew Kamholz ,  Caicai Wu

IPC分类号： B02D1100

CPC分类号： B01D11/0492 ,  A61M1/14 ,  B01D11/0496 ,  B01L3/5027 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0487 ,  G01N15/1484 ,  Y10T436/25375

摘要： This invention provides microfabricated systems for extraction of desired particles from a sample stream containing desired and undesired particles. The sample stream is placed in laminar flow contact with an extraction stream under conditions in which inertial effects are negligible. The contact between the two streams is maintained for a sufficient period of time to allow differential transport of the desired particles from the sample stream into the extraction stream. In a preferred embodiment the differential transport mechanism is diffusion. The extraction system of this invention coupled to a microfabricated diffusion-based mixing device and/or sensing means allows picoliter quantities of fluid to be processed or analyzed on devices no larger than silicon wafers. Such diffusion-based mixing or sensing devices are preferably channel cell systems for detecting the presence and/or measuring the quantity of analyte particles in a sample stream.

摘要翻译： 本发明提供了用于从含有所需和不需要的颗粒的样品流中提取所需颗粒的微制造系统。 在惯性效应可忽略的条件下，将样品流与提取流层流接触。 保持两个物流之间的接触足够的时间，以便将期望的颗粒从样品流中差分输送到萃取物流中。 在优选实施例中，差分传送机构是扩散。 本发明的提取系统耦合到基于微制造的基于扩散的混合装置和/或感测装置允许在不大于硅晶片的装置上处理或分析皮升数量的流体。 这种基于扩散的混合或感测装置优选是用于检测样品流中分析物颗粒的存在和/或测量量的通道电池系统。

公开(公告)号：US5972710A

公开(公告)日：1999-10-26

申请号：US829679

申请日：1997-03-31

申请人： Bernhard H. Weigl ,  Paul Yager ,  James P. Brody ,  Mark R. Holl ,  Margaret Kenny ,  David Schutte ,  Gregory Hixson ,  M. Diane Zebert ,  Andrew Kamholz ,  Caicai Wu ,  Eric Altendorf

发明人： Bernhard H. Weigl ,  Paul Yager ,  James P. Brody ,  Mark R. Holl ,  Margaret Kenny ,  David Schutte ,  Gregory Hixson ,  M. Diane Zebert ,  Andrew Kamholz ,  Caicai Wu ,  Eric Altendorf

IPC分类号： G01N37/00 ,  B01F5/04 ,  B01F13/00 ,  B01J19/00 ,  B01L3/00 ,  G01N15/06 ,  G01N15/14 ,  G01N30/00 ,  G01N30/38 ,  G01N30/60 ,  G01N30/74 ,  G01N31/20 ,  G01N35/08 ,  G01N21/64

CPC分类号： B01F5/0403 ,  B01F13/0059 ,  B01F13/0093 ,  B01J19/0093 ,  B01L3/502776 ,  G01N30/0005 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00952 ,  B01L2200/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487 ,  G01N15/14 ,  G01N30/38 ,  G01N30/6095 ,  G01N30/74 ,  G01N35/085 ,  Y10S366/01 ,  Y10T436/25375 ,  Y10T436/2575

摘要： A channel-cell system is provided for detecting the presence and/or measuring the presence of analyte particles in a sample stream comprising: a) a laminar flow channel; b) two inlets in fluid connection with the laminar flow channel for respectively conducting into the laminar flow channel (1) an indicator stream which may comprise an indicator substance which indicates the presence of the analyte particles by a detectable change in property when contacted with the analyte particles, and (2) the sample stream; c) wherein the laminar flow channel has a depth sufficiently small to allow laminar flow of the streams and a length sufficient to allow particles of the analyte to diffuse into the indicator stream to the substantial exclusion of the larger particles in the sample stream to form a detection area; and d) an outlet for conducting the streams out of the laminar flow channel to form a single mixed stream.

摘要翻译： 提供了一种通道细胞系统，用于检测样品流中存在和/或测量分析物颗粒的存在，其包括：a）层流通道; b）与层流通道流体连接的两个入口，用于分别导入层流通道（1）中，指示物流可包含指示物质，其指示物质与当与 分析物颗粒，和（2）样品流; c）其中层流通道具有足够小的深度以允许流的层流，并且足够的长度允许分析物的颗粒扩散到指示物流中，从而基本排除样品流中的较大颗粒，形成 检测区; 和d）用于将物流从层流通道导出以形成单一混合流的出口。