公开(公告)号：US12259308B2

公开(公告)日：2025-03-25

申请号：US17793208

申请日：2021-01-15

Applicant: HORIBA ABX SAS ,  UNIVERSITE DE MONTPELLIER ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)

Inventor： Pierre Taraconat ,  Damien Isebe ,  Simon Mendez ,  Franck Nicoud

IPC: G01N15/12 ,  G01N15/01 ,  G01N33/487

Abstract: A medical analysis device with cellular impedance signal processing features a memory arranged to receive pulse data sets, with each pulse data set including impedance value data that are associated each time with a time marker. The pulse data sets together represent a curve of cellular impedance values that are measured as a cell passes through a polarised opening. The device further includes a classifier in communication with the memory. The classifier may feature a convolutional neural network that receives the pulse data sets as input. The classifier is provided with at least one convolutional layer, which convolutional layer has a depth greater than or equal to 3, and at least two fully connected layers, in addition to an output layer rendering a cell classification from which a pulse data set is derived.

公开(公告)号：US20200041513A1

公开(公告)日：2020-02-06

申请号：US16303598

申请日：2017-05-22

Applicant: HORIBA ABX SAS ,  CENTRE HOSPITALIER UNIVERSITAIRE DE MONTPELLIER ,  INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM) ,  UNIVERSITE DE MONTPELLIER

Inventor： Guilhem REQUIRAND ,  Bernard KLEIN ,  Thierry REME ,  Jérôme MOREAUX ,  Sébastien RAIMBAULT ,  Philippe NERIN ,  Rosalie PLANTEFEVE ,  Elina ALATERRE ,  Jean-Michel GARCIA

IPC: G01N33/574 ,  G01N15/14 ,  G01N33/50

Abstract: The invention relates to a method for detecting, by means of flow cytometry, the presence of normal plasma cells and tumoral plasma cells in a sample of cells from a patient.

公开(公告)号：US10107801B2

公开(公告)日：2018-10-23

申请号：US14775886

申请日：2014-03-11

Applicant: HORIBA ABX SAS ,  Jerome Bibette

Inventor： Aurelien Daynes ,  Gilles Cauet ,  Jean-Philippe Gineys ,  Philippe Nerin ,  Jerome Bibette

IPC: G01N33/537 ,  G01N33/558 ,  G01N33/543 ,  G01N33/569

Abstract: The present invention relates to a flow assay method in a liquid medium for an object (or element) of interest via the formation of aggregates of particles that are surface-functionalized by at least one functionalizing molecule, or receptor, specific for said object of interest.

公开(公告)号：US20250076290A1

公开(公告)日：2025-03-06

申请号：US18722357

申请日：2022-12-13

Applicant: HORIBA ABX SAS

Inventor： Aurélien DAYNES ,  Nevzat TEMUROK

IPC: G01N33/543

Abstract: Method for assaying a target analyte in a biological sample in liquid medium, comprising: contacting the sample with first magnetic particles bearing a first receptor specific to a first analyte site of attachment to form first complexes; applying a first magnetic field to locally combine the formed complexes formed and optionally to agglomerate interfering complexes to form interfering aggregates; negating the applied magnetic field; adding second magnetic particles to a liquid medium that bear a second receptor specific to a second analyte site of attachment; measuring a first quantity of interfering aggregates; applying a second magnetic field to form second complexes; measuring a second quantity of the collective amount of interfering aggregates and second complexes to determine an amount of formed second complexes as a function of the first quantity, and deducing the amount of analyte present in the sample and, optionally, the amount of interfering analyte.

公开(公告)号：US20200011888A1

公开(公告)日：2020-01-09

申请号：US16479088

申请日：2018-01-17

Applicant: HORIBA ABX SAS

Inventor： Florent BEAUDUCEL ,  Philippe BENEZETH ,  Thibault CRES

IPC: G01N35/00 ,  G01N35/04 ,  B01L9/06

Abstract: A biological analysis system comprising at least one inlet and one outlet, at least two biological analysis devices connected to one another by a conveyor defining a closed circuit, each biological analysis device comprising a region for the exchange of tube holding racks with the conveyor. The conveyor and the at least one inlet of the biological analysis system comprise a reader of an identifier of a tube holding rack which reader is designed to communicate an identifier it has read to a controller of the biological analysis system, which controller is designed to apply a specific treatment to a tube holding rack identified by the reader of the conveyor and the identifier of which has not previously been read by the reader of the at least one inlet of the biological analysis system.

公开(公告)号：US10481076B2

公开(公告)日：2019-11-19

申请号：US15560694

申请日：2016-03-23

Applicant: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES ,  IPRASENSE SAS ,  HORIBA ABX SAS

Inventor： Cedric Allier ,  Geoffrey Esteban ,  Lionel Herve

IPC: G01N15/14 ,  C12M1/34 ,  G01N21/45 ,  G01N21/47 ,  G01N21/51 ,  G03H1/08 ,  G06K9/00 ,  G06T7/00 ,  G03H1/04 ,  G01N33/483 ,  G01N15/10 ,  G01N21/17 ,  G03H1/00 ,  G01N15/00

Abstract: A method for identifying a state of a cell contained in a sample, including: illuminating the sample using a light source by producing an incident light wave propagating toward the sample; then acquiring, using a matrix-array photodetector, an image of the sample, the sample being placed between the light source and the matrix-array photodetector such that the matrix-array photodetector is exposed to a light wave resulting from interference between the incident light wave and a diffraction wave produced by each cell; applying a numerical reconstruction algorithm to the image acquired by the matrix-array photodetector, to estimate a characteristic quantity of the light wave reaching the matrix-array detector, at a plurality of distances from the matrix-array photodetector. The value of the characteristic quantity, or its variation as a function of distance, allows the state of the cell to be determined from among predetermined states.

公开(公告)号：US20190160442A1

公开(公告)日：2019-05-30

申请号：US16320956

申请日：2017-07-25

Applicant: HORIBA ABX SAS

Inventor： Jean Emmanuel LARAVINE ,  Charlène MUZELLIER

IPC: B01F11/00

Abstract: A device for shaking and sampling biological liquids that is able to take a sample of a biological liquid in a tube including a shaker designed to mix a rack holding one or more tubes by performing a succession of tilting movements between two shaking positions. The shaker is also arranged to tilt beyond the shaking position furthest away from the insertion position of a rack to discharge same by gravity.

公开(公告)号：US20190101482A1

公开(公告)日：2019-04-04

申请号：US16086922

申请日：2017-03-22

Applicant: COMMISSARIAT Á L'ÉNERGIE ATOMIQUE ET AUX ÉNERGIES AL TERNATIVES ,  HORIBA ABX SAS ,  IPRASENSE SAS

Inventor： Cedric ALLIER ,  Anais ALI CHERIF ,  Pierre BLANDIN ,  Geoffrey ESTEBAN ,  Lionel HERVE ,  Damien ISEBE

IPC: G01N15/02 ,  G01N15/14 ,  G03H1/08

Abstract: A method for holographic characterization of a particle contained in a sample, based on an image, or hologram, of the sample obtained by an image sensor when the sample is illuminated by a light source. The hologram is the subject of a holographic reconstruction, to obtain a reference complex image, representative of the light wave transmitted by the sample in a reconstruction plane. A holographic propagation operator is applied to the reference complex image, to obtain a plurality of secondary complex images, from which a profile is determined describing the change in an optical feature of the light wave transmuted by the sample along the axis of propagation of the light wave.

公开(公告)号：US20180340948A1

公开(公告)日：2018-11-29

申请号：US15775214

申请日：2016-11-09

Applicant: HORIBA ABX SAS

Inventor： Sylvain ANDLAUER ,  Florent BEAUDUCEL ,  Philippe BENEZETH

IPC: G01N35/00 ,  G01N1/00

CPC classification number: G01N35/00732 ,  G01N1/00 ,  G01N35/0092 ,  G01N35/0099 ,  G01N35/1083 ,  G01N2001/002 ,  G01N2035/00524 ,  G01N2035/041 ,  G01N2035/0412 ,  G01N2035/0472 ,  G01N2035/0493 ,  G01N2035/1058

Abstract: A device for agitating and collecting biological liquid samples comprises an agitator of racks of tubes and a sampling apparatus capable of collecting a biological liquid sample in a tube. The device also comprises a scheduler arranged to specify an order of sampling from the tubes independently of the order in which the tubes are positioned in the respective racks and the order in which the racks are inserted into the device. The scheduler is arranged to control the agitator and the sampling apparatus to process the tubes in accordance with the sampling order.

公开(公告)号：US09448175B2

公开(公告)日：2016-09-20

申请号：US14423030

申请日：2013-08-19

Applicant: HORIBA ABX SAS

Inventor： Alexandra Urankar ,  Philippe Nerin ,  Moustapha Hariche ,  Gilles Cauet

IPC: G01N21/64 ,  G01N21/27 ,  G01N15/14 ,  G01N33/49 ,  G01N35/00 ,  G01N15/10

CPC classification number: G01N21/6486 ,  G01N15/147 ,  G01N21/274 ,  G01N21/6428 ,  G01N33/49 ,  G01N35/00663 ,  G01N2015/1006 ,  G01N2035/00673 ,  G01N2201/121

Abstract: The present invention relates to a method for compensating for the breakdown of a reagent stored in an aqueous phase comprising at least one fluorescent compound and enabling the identification of particles, including the steps of: (i) measuring the fluorescence level FLUOm(t) of particles marked with said reagent; (ii) measuring the absorbance at at least one wavelength of a solution of said reagent, at a time t close to the time of said fluorescence level FLUOm(t) measurements, so as to determine at least one current optical density DO(t) of the reagent; and (iii) calculating a correction of the fluorescent level measurements using said at least one current optical density DO(t) and at least one initial optical density DO(0) of the reagent that has not been broken down. The invention also relates to a biological analysis device implementing the method.

Abstract translation: 本发明涉及一种用于补偿存储在包含至少一种荧光化合物的水相中的试剂的分解的方法，并且能够鉴定颗粒，包括以下步骤：（i）测量荧光水平FLUOm（t） 用所述试剂标记的颗粒; （ii）在接近所述荧光水平FLUOm（t）测量时间的时间t测量所述试剂溶液的至少一个波长处的吸光度，以便确定至少一个当前光密度DO（t） 的试剂; 以及（iii）使用所述至少一个当前光密度DO（t）和至少一个未被分解的试剂的初始光密度DO（0）来计算荧光水平测量的校正。 本发明还涉及实现该方法的生物分析装置。