公开(公告)号：US09448175B2

公开(公告)日：2016-09-20

申请号：US14423030

申请日：2013-08-19

Applicant: HORIBA ABX SAS

Inventor： Alexandra Urankar ,  Philippe Nerin ,  Moustapha Hariche ,  Gilles Cauet

IPC: G01N21/64 ,  G01N21/27 ,  G01N15/14 ,  G01N33/49 ,  G01N35/00 ,  G01N15/10

CPC classification number: G01N21/6486 ,  G01N15/147 ,  G01N21/274 ,  G01N21/6428 ,  G01N33/49 ,  G01N35/00663 ,  G01N2015/1006 ,  G01N2035/00673 ,  G01N2201/121

Abstract: The present invention relates to a method for compensating for the breakdown of a reagent stored in an aqueous phase comprising at least one fluorescent compound and enabling the identification of particles, including the steps of: (i) measuring the fluorescence level FLUOm(t) of particles marked with said reagent; (ii) measuring the absorbance at at least one wavelength of a solution of said reagent, at a time t close to the time of said fluorescence level FLUOm(t) measurements, so as to determine at least one current optical density DO(t) of the reagent; and (iii) calculating a correction of the fluorescent level measurements using said at least one current optical density DO(t) and at least one initial optical density DO(0) of the reagent that has not been broken down. The invention also relates to a biological analysis device implementing the method.

Abstract translation: 本发明涉及一种用于补偿存储在包含至少一种荧光化合物的水相中的试剂的分解的方法，并且能够鉴定颗粒，包括以下步骤：（i）测量荧光水平FLUOm（t） 用所述试剂标记的颗粒; （ii）在接近所述荧光水平FLUOm（t）测量时间的时间t测量所述试剂溶液的至少一个波长处的吸光度，以便确定至少一个当前光密度DO（t） 的试剂; 以及（iii）使用所述至少一个当前光密度DO（t）和至少一个未被分解的试剂的初始光密度DO（0）来计算荧光水平测量的校正。 本发明还涉及实现该方法的生物分析装置。

公开(公告)号：US20150241352A1

公开(公告)日：2015-08-27

申请号：US14423030

申请日：2013-08-19

Applicant: HORIBA ABX SAS

Inventor： Alexandra Urankar ,  Philippe Nerin ,  Moustapha Hariche ,  Gilles Cauet

IPC: G01N21/64 ,  G01N33/49

CPC classification number: G01N21/6486 ,  G01N15/147 ,  G01N21/274 ,  G01N21/6428 ,  G01N33/49 ,  G01N35/00663 ,  G01N2015/1006 ,  G01N2035/00673 ,  G01N2201/121

Abstract: The present invention relates to a method for compensating for the breakdown of a reagent stored in an aqueous phase comprising at least one fluorescent compound and enabling the identification of particles, including the steps of: (i) measuring the fluorescence level FLUOm(t) of particles marked with said reagent; (ii) measuring the absorbance at at least one wavelength of a solution of said reagent, at a time t close to the time of said fluorescence level FLUOm(t) measurements, so as to determine at least one current optical density DO(t) of the reagent; and (iii) calculating a correction of the fluorescent level measurements using said at least one current optical density DO(t) and at least one initial optical density DO(0) of the reagent that has not been broken down. The invention also relates to a biological analysis device implementing the method.

Abstract translation: 本发明涉及一种用于补偿存储在包含至少一种荧光化合物的水相中的试剂的分解的方法，并且能够鉴定颗粒，包括以下步骤：（i）测量荧光水平FLUOm（t） 用所述试剂标记的颗粒; （ii）在接近所述荧光水平FLUOm（t）测量时间的时间t测量所述试剂溶液的至少一个波长处的吸光度，以便确定至少一个当前光密度DO（t） 的试剂; 以及（iii）使用所述至少一个当前光密度DO（t）和至少一个未被分解的试剂的初始光密度DO（0）来计算荧光水平测量的校正。 本发明还涉及实现该方法的生物分析装置。

公开(公告)号：US10073086B2

公开(公告)日：2018-09-11

申请号：US14376270

申请日：2013-01-29

Applicant: HORIBA ABX SAS

Inventor： Jèrôme Bibette ,  Philippe Nerin ,  Jean-Philippe Gineys ,  Gilles Cauet

IPC: G01N33/49 ,  G01N33/50 ,  G01N1/38 ,  G01N35/00 ,  G01N35/10 ,  G01N21/82 ,  G01N15/14

CPC classification number: G01N33/5094 ,  G01N1/38 ,  G01N35/0098 ,  G01N35/10 ,  G01N2015/1486 ,  G01N2021/825 ,  G01N2333/46 ,  G01N2333/4737

Abstract: The present invention concerns a device for analyzing biological parameters from a sample (6) comprising (i) first transferring means (5, 20, 25), (ii) first preparing means (7), (iii) means for measuring cellular components (8), (iv) second preparing means (10, 11, 22, 23, 24) capable of carrying out, on a sample from the first preparing means (7), at least one dilution with an assay reagent (R3) comprising particles functionalized at the surface with at least one ligand specific to at least one analyte of interest, (v) immunodetection measurement means (30, 31) capable of assaying at least one analyte of interest by measuring the aggregation of functionalized particles, said device further comprising (i) second transferring means (4, 21, 22, 26) at least partially separate from the first transferring means (5, 20, 25) and (ii) means for applying a magnetic field (28) capable of causing, by magnetic interaction, an acceleration of the aggregation of said functionalized particles, which comprise magnetic colloidal particles. The invention also concerns a method implemented in said device.

公开(公告)号：US10107801B2

公开(公告)日：2018-10-23

申请号：US14775886

申请日：2014-03-11

Applicant: HORIBA ABX SAS ,  Jerome Bibette

Inventor： Aurelien Daynes ,  Gilles Cauet ,  Jean-Philippe Gineys ,  Philippe Nerin ,  Jerome Bibette

IPC: G01N33/537 ,  G01N33/558 ,  G01N33/543 ,  G01N33/569

Abstract: The present invention relates to a flow assay method in a liquid medium for an object (or element) of interest via the formation of aggregates of particles that are surface-functionalized by at least one functionalizing molecule, or receptor, specific for said object of interest.

公开(公告)号：US20140377771A1

公开(公告)日：2014-12-25

申请号：US14376270

申请日：2013-01-29

Applicant: HORIBA ABX SAS

Inventor： Jérôme Bibette ,  Philippe Nerin ,  Jean-Philippe Gineys ,  Gilles Cauet

IPC: G01N33/50

CPC classification number: G01N33/5094 ,  G01N1/38 ,  G01N35/0098 ,  G01N35/10 ,  G01N2015/1486 ,  G01N2021/825 ,  G01N2333/46 ,  G01N2333/4737

Abstract: The present invention concerns a device for analyzing biological parameters from a sample (6) comprising (i) first transferring means (5, 20, 25), (ii) first preparing means (7), (iii) means for measuring cellular components (8), (iv) second preparing means (10, 11, 22, 23, 24) capable of carrying out, on a sample from the first preparing means (7), at least one dilution with an assay reagent (R3) comprising particles functionalized at the surface with at least one ligand specific to at least one analyte of interest, (v) immunodetection measurement means (30, 31) capable of assaying at least one analyte of interest by measuring the aggregation of functionalized particles, said device further comprising (i) second transferring means (4, 21, 22, 26) at least partially separate from the first transferring means (5, 20, 25) and (ii) means for applying a magnetic field (28) capable of causing, by magnetic interaction, an acceleration of the aggregation of said functionalized particles, which comprise magnetic colloidal particles. The invention also concerns a method implemented in said device.

Abstract translation: 本发明涉及一种用于分析来自样品（6）的生物学参数的装置，包括（i）第一转移装置（5,20,25），（ii）第一制备装置（7），（iii）用于测量细胞组分的装置 8），（iv）能够在来自第一制备装置（7）的样品上进行至少一种稀释度的测定试剂（R3）的第二制备装置（10,11,22,23,24），其包含颗粒 在表面上官能化至少一种对至少一种目的分析物特异性的配体;（v）能够通过测量官能化颗粒的聚集来测定所述至少一种目的分析物的免疫检测测量装置（30,31），所述装置还包括 （i）与第一传送装置（5,20,25）至少部分分离的第二传送装置（4,21,22,26）和（ii）用于施加磁场（28）的装置，该磁场能够通过磁 相互作用，所述功能化颗粒的聚集加速 h包含磁性胶体颗粒。 本发明还涉及在所述设备中实现的方法。