利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

8 条记录 (耗时 0.011 秒)

    Method for detecting redispersion of beads
    5.
    发明授权
    Method for detecting redispersion of beads 有权
    检测珠子再分散的方法

    公开(公告)号：US08339608B2

    公开(公告)日：2012-12-25

    申请号：US12740148

    申请日：2008-10-30

    申请人： Harold Johannes Antonius Brans ,  Albert Hendrik Jan Immink ,  Hendrik Sibolt Van Damme ,  Mara Johanna Jacoba Sijbers

    发明人： Harold Johannes Antonius Brans ,  Albert Hendrik Jan Immink ,  Hendrik Sibolt Van Damme ,  Mara Johanna Jacoba Sijbers

    IPC分类号： G01N21/55

    CPC分类号： G01N21/552 ,  G01N15/0612 ,  G01N21/648 ,  G01N2015/0693

    摘要： A method of detecting redispersion of particles into a solution using for example FTIR. The method including providing a sensor surface with dry particles; illuminating the sensor surface with light along a first optical path and detecting the light reflected by the sensor surface; providing a liquid to a volume in contact with the sensor surface; and detecting the reflected light while the dry particles redisperse into the liquid. The angle between the first optical path and the sensor surface fulfils the condition of total internal reflection. Further, an FTIR cartridge may be provided for use in said method. The cartridge including a sensor surface accessible for FTIR detection including at least one binding area wherein label particles are situated.

    摘要翻译： 使用例如FTIR检测颗粒再次分散到溶液中的方法。 该方法包括向传感器表面提供干燥颗粒; 用沿着第一光路的光照射传感器表面并检测由传感器表面反射的光; 向与传感器表面接触的体积提供液体; 并且当干燥颗粒再分散到液体中时检测反射光。 第一光路和传感器表面之间的角度满足全内反射的条件。 此外，可以提供用于所述方法的FTIR盒。 该盒包括可用于FTIR检测的传感器表面，包括标签颗粒所在的至少一个结合区域。

    Moving droplet diagnostic assay
    7.
    发明授权
    Moving droplet diagnostic assay 有权
    移动液滴诊断测定

    公开(公告)号：US08586349B2

    公开(公告)日：2013-11-19

    申请号：US10344226

    申请日：2001-08-06

    申请人： Thomas Augustinus Maria Beumer ,  Petrus Franciscus Hendrikus M. Verheijden ,  Hendrik Sibolt van Damme

    发明人： Thomas Augustinus Maria Beumer ,  Petrus Franciscus Hendrikus M. Verheijden ,  Hendrik Sibolt van Damme

    IPC分类号： G01N33/00 ,  G01N33/53 ,  C12Q1/00 ,  C12Q1/68 ,  D21C3/00

    CPC分类号： G01N33/54393 ,  G01N33/54366 ,  Y10T436/2525

    摘要： The present invention concerns a method for the detection of an analyte in a droplet of fluid comprising the steps of providing a solid phase with a hydrophobic surface comprising at least one capture zone on which at least one binding agent with affinity for the analyte is immobilized, applying said droplet to the surface of said solid phase, applying a force that makes the droplet travel along the surface of said solid phase along a predetermined path thereby allowing the droplet to repeatedly contact said binding agent on the capture zone, applying conditions wherein said analyte is allowed to bind to said binding agent and detecting a complex of analyte and binding reagent at the position of the capture zone. The effect of a moving droplet is that the reactants in the droplet are well mixed which eliminates the risk of diffusion limitation. Such advantageous mixing is not obtained with conventional assays in which the surface is continuously exposed to the liquid. The use of a droplet eliminates the need for external mixing and also circumvents the need to dilute the sample in order to obtain sufficient volume to wet a large surface area.

    摘要翻译： 本发明涉及一种用于检测流体液滴中的分析物的方法，包括以下步骤：向固相提供包含至少一个捕获区的疏水表面，在该捕获区上固定有至少一种对分析物具有亲和力的结合剂， 将所述液滴施加到所述固相的表面，施加使液滴沿着预定路径沿着所述固相的表面行进的力，从而允许液滴在捕获区上反复接触所述粘合剂，施加条件，其中所述分析物 被允许结合到所述结合剂并且在捕获区的位置检测分析物和结合试剂的复合物。 移动液滴的作用是液滴中的反应物充分混合，消除了扩散限制的风险。 用表面连续暴露于液体的常规测定法不能获得这种有利的混合。 使用液滴消除了对外部混合的需要，并且还避免了稀释样品的需要，以便获得足够的体积以润湿大的表面积。