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    METHOD FOR PRODUCING LACTIC ACID AND METHOD FOR PRODUCING POLYLACTIC ACID
    3.
    发明申请
    METHOD FOR PRODUCING LACTIC ACID AND METHOD FOR PRODUCING POLYLACTIC ACID 审中-公开
    生产乳酸的方法和生产聚酸的方法

    公开(公告)号：US20110263811A1

    公开(公告)日：2011-10-27

    申请号：US13142062

    申请日：2009-12-25

    申请人： Kenji Sawai ,  Hideki Sawai ,  Takashi Mimitsuka ,  Ito Masateru ,  Katsushige Yamada ,  Kenji Kawamura ,  Shin-ichi Minegishi ,  Izumi Nakagawa ,  Tatsuya Nagano

    发明人： Kenji Sawai ,  Hideki Sawai ,  Takashi Mimitsuka ,  Ito Masateru ,  Katsushige Yamada ,  Kenji Kawamura ,  Shin-ichi Minegishi ,  Izumi Nakagawa ,  Tatsuya Nagano

    IPC分类号： C08G63/78 ,  C07D319/12 ,  C12P7/56

    摘要： Lactic acid is obtained by a method including (A) a step of continuous fermentation wherein a fermentation culture medium of a microorganism having an ability of lactic acid fermentation is filtered through a porous membrane having an average pore size of not less than 0.01 μm and less than 1 μm with a transmembrane pressure difference within the range of 0.1 to 20 kPa, and the permeate is collected, while retaining the non-permeated liquid in or returning the non-permeated liquid to the culture, and adding a fermentation feedstock to the culture; (B) a step of filtering the permeate obtained in Step (A) through a nanofiltration membrane; and (C) a step of distilling the permeate obtained in Step (B) under a pressure of not less than 1 Pa and not more than atmospheric pressure, at 25° C. to 200° C. to recover lactic acid.

    摘要翻译： 乳酸通过以下方法获得：（A）连续发酵步骤，其中具有乳酸发酵能力的微生物的发酵培养基通过平均孔径不小于0.01μm的多孔膜过滤 超过1μm，跨膜压力差在0.1〜20kPa的范围内，并收集渗透物，同时将非渗透液体保留在或将非渗透液体返回到培养物中，并向培养物中加入发酵原料 ; （B）通过纳滤膜对步骤（A）中得到的渗透物进行过滤的工序; 和（C）在25℃至200℃下，在不低于1Pa且不大于大气压的压力下，将步骤（B）中得到的渗透物蒸馏的步骤，以回收乳酸。

    Method for producing cadaverine
    5.
    发明授权
    Method for producing cadaverine 有权
    生成尸胺的方法

    公开(公告)号：US08999681B2

    公开(公告)日：2015-04-07

    申请号：US13991929

    申请日：2011-12-08

    申请人： Nanami Sasaki ,  Takashi Mimitsuka ,  Hideki Sawai ,  Kenji Sawai

    发明人： Nanami Sasaki ,  Takashi Mimitsuka ,  Hideki Sawai ,  Kenji Sawai

    IPC分类号： C12P13/00 ,  C12N9/04 ,  C12N9/88

    CPC分类号： C12P13/001 ,  C12N9/0006 ,  C12N9/88 ,  C12Y101/01003 ,  C12Y401/01018

    摘要： A method of producing cadaverine is more efficient and at a higher yield than production methods by the conventional fermentation methods. The method includes culturing coryneform bacterium/bacteria having a resistance to a pH of 5.5 or less. Preferably, the coryneform bacterium/bacteria has/have lysine decarboxylase activity and, preferably, the coryneform bacterium/bacteria has/have homoserine auxotrophy and/or a resistance to S-(2-aminoethyl)-L-cysteine.

    摘要翻译： 与通过常规发酵方法的生产方法相比，生产尸胺的方法更有效并且产量高。 该方法包括培养耐pH为5.5以下的棒状细菌/细菌。 优选地，棒状细菌/细菌具有/具有赖氨酸脱羧酶活性，优选棒状细菌/细菌具有高丝氨酸营养缺陷型和/或对S-（2-氨基乙基）-L-半胱氨酸的抗性。

    METHOD FOR PRODUCING A CHEMICAL PRODUCT AND CONTINUOUS FERMENTATION APPARATUS
    6.
    发明申请
    METHOD FOR PRODUCING A CHEMICAL PRODUCT AND CONTINUOUS FERMENTATION APPARATUS 审中-公开
    生产化学产品和连续发酵装置的方法

    公开(公告)号：US20110177551A1

    公开(公告)日：2011-07-21

    申请号：US13121727

    申请日：2009-09-16

    申请人： Takashi Mimitsuka ,  Kentaro Ishii ,  Ken Morita ,  Masashi Higasa ,  Kenji Sawai ,  Hideki Sawai ,  Katsushige Yamada ,  Shinichi Minegishi

    发明人： Takashi Mimitsuka ,  Kentaro Ishii ,  Ken Morita ,  Masashi Higasa ,  Kenji Sawai ,  Hideki Sawai ,  Katsushige Yamada ,  Shinichi Minegishi

    IPC分类号： C12P1/00 ,  C12M1/12

    CPC分类号： C12P7/56 ,  C12M29/18 ,  C12M47/10 ,  C12N9/00 ,  C12P1/00 ,  C12P7/02 ,  C12P7/40 ,  C12P13/001 ,  C12P13/04 ,  C12P13/08 ,  C12P19/34 ,  C12P21/02

    摘要： To control flow velocity of a culture liquid inside a membrane separation tank without giving influences to culture conditions in the fermentation tank, and also suppress precipitation of microorganisms or culture cells so that the production efficiency of the chemical product can be improved, in a method for producing a chemical product including the steps of: cultivating microorganisms or culture cells in a fermentation tank; transferring a culture liquid from the fermentation tank to a membrane separation tank so as to filter the culture liquid through a separation membrane; and collecting a fermentation product from a filtration liquid as the chemical product while refluxing an unfiltered culture liquid that has not been filtered so as to be joined to the culture liquid on an upstream side of the membrane separation tank, one portion of the culture liquid to be transferred from the fermentation tank is allowed to bypass the membrane separation tank depending on a pressure at the culture liquid flow-in side of the membrane separation tank.

    摘要翻译： 为了控制膜分离槽内的培养液的流速，不影响发酵罐中的培养条件，并且还抑制微生物或培养细胞的沉淀，从而可以提高化学品的生产效率 生产化学产品，包括以下步骤：在发酵罐中培养微生物或培养细胞; 将培养液从发酵罐转移到膜分离罐中，以通过分离膜过滤培养液; 并将来自过滤液的发酵产物作为化学产品收集，同时回流未过滤的未过滤的培养液，以便在膜分离罐的上游侧与培养液接合，将一部分培养液转移至 根据膜分离罐的培养液流入侧的压力，从发酵罐转移允许绕过膜分离罐。

    POLYPEPTIDE HAVING D-LACTATE DEHYDROGENASE ACTIVITY, POLYNUCLEOTIDE ENCODING THE POLYPEPTIDE, AND PROCESS FOR PRODUCTION OF D-LACTIC ACID
    9.
    发明申请
    POLYPEPTIDE HAVING D-LACTATE DEHYDROGENASE ACTIVITY, POLYNUCLEOTIDE ENCODING THE POLYPEPTIDE, AND PROCESS FOR PRODUCTION OF D-LACTIC ACID 有权
    具有D-脱水脱氢酶活性的多肽，编码多肽的多核苷酸以及D-乳酸的生产方法

    公开(公告)号：US20120070871A1

    公开(公告)日：2012-03-22

    申请号：US13375882

    申请日：2010-06-02

    申请人： Kenji Sawai ,  Kazumi Suda ,  Hideki Sawai ,  Katsushige Yamada ,  Junya Yamagishi

    发明人： Kenji Sawai ,  Kazumi Suda ,  Hideki Sawai ,  Katsushige Yamada ,  Junya Yamagishi

    IPC分类号： C12P7/56 ,  C12N1/19 ,  C12N15/63 ,  C12N9/04 ,  C12N15/53

    CPC分类号： C12P7/56 ,  C12N9/0006

    摘要： Highly productive D-lactic acid fermentation uses a transformant obtained by introducing into a host cell a polynucleotide encoding a polypeptide according to any one of the following (A) to (C) in such a manner that the polypeptide is expressed, which polypeptide has a D-lactate dehydrogenase activity higher than those of conventional polypeptides: (A) a polypeptide having the amino acid sequence shown in SEQ ID NO:1 or 2; (B) a polypeptide having the same amino acid sequence as shown in SEQ ID NO:1 or 2 except that one or several amino acids are substituted, deleted, inserted and/or added, which polypeptide has a D-lactate dehydrogenase activity; and (C) a polypeptide having an amino acid sequence which has a sequence identity of not less than 80% to the amino acid sequence shown in SEQ ID NO:1 or 2, which polypeptide has a D-lactate dehydrogenase activity.

    摘要翻译： 高生产率D-乳酸发酵使用通过向宿主细胞中引入编码根据下列（A）至（C）中任一项所述的多肽的多核苷酸而获得的转化体，使得多肽表达， D-乳酸脱氢酶活性高于常规多肽的活性：（A）具有SEQ ID NO：1或2所示氨基酸序列的多肽; （B）具有与SEQ ID NO：1或2所示相同氨基酸序列的多肽，不同之处在于一个或几个氨基酸被取代，缺失，插入和/或添加，该多肽具有D-乳酸脱氢酶活性; 和（C）具有与SEQ ID NO：1或2所示的氨基酸序列具有不小于80％的序列同一性的氨基酸序列的多肽，该多肽具有D-乳酸脱氢酶活性。

    LACTIC ACID PRODUCTION METHOD
    10.
    发明申请
    LACTIC ACID PRODUCTION METHOD 有权
    柠檬酸生产方法

    公开(公告)号：US20100190222A1

    公开(公告)日：2010-07-29

    申请号：US12666690

    申请日：2008-06-18

    申请人： Masateru Ito ,  Shin-ichi Minegishi ,  Eri Shimizu ,  Kenji Sawai ,  Yohito Ito ,  Hideki Sawai ,  Katsushige Yamada

    发明人： Masateru Ito ,  Shin-ichi Minegishi ,  Eri Shimizu ,  Kenji Sawai ,  Yohito Ito ,  Hideki Sawai ,  Katsushige Yamada

    IPC分类号： C12P7/56

    CPC分类号： C12P7/56 ,  B01D61/027 ,  B01D71/56 ,  B01D2311/06 ,  B01D2311/18 ,  B01D2311/2669

    摘要： Disclosed is a lactic acid production method by separating lactic acid produced in a culture solution by means of the fermentation culture of a microorganism. Specifically disclosed is a lactic acid production method, which comprises: a step (A) of filtering the culture solution through a nano-filtration membrane; and a step (B) of distilling a lactic-acid-containing solution produced in the step (A) under a pressure ranging from 1 Pa to the atmospheric pressure (inclusive) at a temperature ranging from 25 to 200° C. (inclusive) to collect lactic acid. The method can effectively remove an inorganic salt dissolved in a fermentation culture solution or contained in the fermentation culture solution in the form of a poorly soluble solid material by a simple manipulation, enables to prevent the racemization or oligomerization of lactic acid during the process of producing lactic acid, and therefore can produce lactic acid in a high yield.

    摘要翻译： 公开了通过微生物的发酵培养分离培养液中产生的乳酸的乳酸生产方法。 具体公开的是乳酸的制造方法，其包括：通过纳滤膜过滤培养液的工序（A） 和（B）在步骤（A）中制备的含乳酸的溶液在25Pa至200℃的温度范围内，在1Pa至大气压（含）范围内， 收集乳酸。 该方法可以通过简单的操作有效地除去溶解在发酵培养液中的无机盐或发酵培养液中含有的难溶性固体物质的形式的无机盐，能够防止生产过程中乳酸的外消旋化或低聚反应 乳酸，因此可以高产率生产乳酸。