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公开(公告)号：US20130108773A1
公开(公告)日：2013-05-02
申请号：US13643326
申请日：2011-05-13
申请人： Tetsuji Odani , Hideyo Tadami , Keiichi Okutomi , Ichiro Shibuya
发明人： Tetsuji Odani , Hideyo Tadami , Keiichi Okutomi , Ichiro Shibuya
IPC分类号： A23L1/22
摘要： An object of the present invention is to provide a seasoning composition which is highly safe to the human body, has excellent sweet taste and first taste, and can be used as an alternative to an animal protein hydrolysate without causing any problem. Means for solving the problem is a seasoning composition comprising a yeast extract obtained from yeast fungus bodies cultured under conditions advantageous for production of alanine, wherein the seasoning composition contains 20% by weight or more of free alanine based on free amino acids.
摘要翻译： 本发明的目的是提供对人体高度安全的调味料组合物，具有优异的甜味和第一味道，并且可以用作动物蛋白水解产物的替代物而不引起任何问题。 解决问题的手段是包含从有利于生产丙氨酸的条件下培养的酵母真菌体获得的酵母提取物的调味料组合物，其中所述调味组合物含有20重量％以上的游离丙氨酸，基于游离氨基酸。
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2.发明授权Escherichia coli Candida maltosa Saccharomyces cerevisiae shuttle vectors and method for making 失效大肠杆菌麦芽糖酵母（Saccharomyces cerevisiae）梭菌载体及其制备方法
公开(公告)号：US4879230A
公开(公告)日：1989-11-07
申请号：US813193
申请日：1985-12-24
申请人： Masamichi Takagi , Keiji Yano , Ichiro Shibuya , Minoru Morikawa
发明人： Masamichi Takagi , Keiji Yano , Ichiro Shibuya , Minoru Morikawa
IPC分类号： C12N15/81
CPC分类号： C12N15/81
摘要： The present invention relates to plasmids whose hosts can be Escherichia coli and some kinds of yeasts, namely, shuttle vectors, as well as to processes for producing said plasmids. There are provided in the present invention (1) plasmids containing an autonomously replicating sequence of Candida maltosa, Leu 2 gene derived from Saccharomyces cerevisiae and an ampicillin resistance gene and (2) plasmids further containing a tetracycline resistance gene as well as the genes described in (1).The plasmids (shuttle vectors) of the present invention can be utilized as follows. A useful foreign gene is inserted into plasmids of the present invention; using the resulting new plasid, Escherichia coli is transformed and cultured in order to obtain the plasmid in a large amount; and using this plasmid, Saccharomyces cerevisiae or Candida maltosa as a host is allowed to produce useful substances such as hormones and enzymes on a large scale.
摘要翻译： 本发明涉及其宿主可以是大肠杆菌和一些种类的酵母的质粒，即穿梭载体，以及生产所述质粒的方法。 在本发明中提供了（1）含有自愿复制的假丝酵母（Candida maltosa）的质粒，来源于酿酒酵母的Leu 2基因和氨苄青霉素抗性基因的质粒和（2）进一步含有四环素抗性基因的质粒以及描述于 （1）。 本发明的质粒（穿梭载体）可以如下使用。 将有用的外源基因插入本发明的质粒中; 使用所得的新的质粒，转化和培养大肠杆菌以获得大量的质粒; 使用该质粒，可以将酿酒酵母或麦芽假丝酵母作为宿主大量产生有用的物质如激素和酶。
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公开(公告)号：US09005683B2
公开(公告)日：2015-04-14
申请号：US13128677
申请日：2009-10-30
IPC分类号： A23L1/28 , A23C9/12 , C12N1/00 , C12N1/16 , A23L1/228 , A23L1/30 , A23L1/305 , A23L1/39 , A23L2/56 , C12N1/18
CPC分类号： C12N1/16 , A23L2/56 , A23L23/00 , A23L27/22 , A23L33/145 , A23L33/175 , C12N1/18
摘要： Provided is a method for producing a yeast containing glutamic acid at a high concentration. In the method for culturing a yeast, a yeast in a stationary growth phase is subjected to liquid culture under the conditions that the pH of a liquid medium is 7.5 or higher and lower than 11. After the pH of the liquid medium for the yeast in a stationary growth phase is adjusted to 7.5 or higher and lower than 11, the yeast is further cultured, whereby a yeast with a high glutamic acid content can be produced. In the invention, as the yeast, Saccharomyces cerevisiae or Candida utilis can be used. Therefore, by using the yeast with a high glutamic acid content obtained by the above-said method and the extract obtained by extraction from the yeast, a seasoning composition and a food or drink with a high glutamic acid content can be provided.
摘要翻译： 本发明提供高浓度含有谷氨酸的酵母的制造方法。 在培养酵母的方法中，在液体培养基的pH为7.5以上且低于11的条件下，将静止生长期的酵母进行液体培养。酵母的液体培养基的pH 将固定生长期调整为7.5以上且低于11，进一步培养酵母，由此可以生产具有高谷氨酸含量的酵母。 在本发明中，作为酵母，可以使用酿酒酵母或产朊假丝酵母。 因此，通过使用通过上述方法获得的高谷氨酸含量的酵母和通过从酵母中提取获得的提取物，可以提供具有高谷氨酸含量的调味组合物和食品或饮料。
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公开(公告)号：US20110206823A1
公开(公告)日：2011-08-25
申请号：US13126514
申请日：2009-11-17
申请人： Ichiro Shibuya , Tetsuji Odani
发明人： Ichiro Shibuya , Tetsuji Odani
CPC分类号： C12P13/04 , A23L33/145 , A23L33/175 , C12N1/16 , C12N1/18
摘要： The present invention provides: a method for producing an amino acid-rich yeast containing free amino acid in high concentration; a method for producing an amino acid-rich yeast, which includes liquid-culturing a yeast in a stationary phase of proliferation wherein the pH of a liquid medium is ≧7.5 and
摘要翻译： 本发明提供一种含有高浓度游离氨基酸的富含氨基酸的酵母的制造方法。 一种富含氨基酸的酵母的制造方法，其特征在于，在液体培养基的pH为7.5以上，增殖稳定期为液体培养酵母的情况下， 一种生产富含氨基酸的酵母的方法，其包括将液体培养基的pH调节至≥7.5和<11，并在该pH范围内进一步培养酵母; 一种生产富含氨基酸的酵母的方法，其中酵母是酿酒酵母或产朊假丝酵母; 通过上述方法得到的富含氨基酸的酵母; 富含氨基酸的酵母提取物，其中游离氨基酸的量为每干酵母细胞7.5至18.0重量％; 和从上述富含氨基酸的酵母提取的富含氨基酸的酵母提取物。
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5.发明授权Disposable body fluid filter unit, disposable body fluid sucking device, and body fluid sucking source 失效一次性体液过滤器单元，一次性体液吸引装置和体液吸引源公开(公告)号：US06589219B1
公开(公告)日：2003-07-08
申请号：US09914410
申请日：2001-08-28
申请人： Ichiro Shibuya
发明人： Ichiro Shibuya
IPC分类号： A61M100
CPC分类号： A61M1/0001 , A61G7/0503 , A61M1/0049 , A61M1/0052 , A61M1/0056 , A61M2205/076 , A61M2209/082
摘要： A disposable body fluid filter unit 10 of the present invention comprises a suction inlet 14 connected to a suction tube 20 for suctioning body fluids 100; a collection chamber 18 for storing the body fluids 100 sucked in together with air after being separated from air; a filter 12 for allowing introduced air to pass through; and a collection inlet 16 communicating with a suction source 24, wherein the suction inlet 14 is positioned at one end of the substantially cylindrical-shape collection chamber 18, the filter 12 is positioned at the other end that is opposite to the one end, and a collection piper 23 is provided for guiding air from the chamber 18 to the collection inlet 14 through the filter 12.
摘要翻译： 本发明的一次性体液过滤器单元10包括连接到抽吸管20以吸引体液100的吸入口14; 收集室18，用于储存在与空气分离后与空气一起吸入的体液100; 用于允许引入的空气通过的过滤器12; 以及与抽吸源24连通的收集入口16，其中吸入口14位于大致圆筒形收集室18的一端，过滤器12位于与一端相对的另一端，并且 收集吹瓶器23用于通过过滤器12将空气从室18引导到收集入口14。
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公开(公告)号：US4670979A
公开(公告)日：1987-06-09
申请号：US732308
申请日：1985-05-09
申请人： Shigeru Yoshino , Junzo Ueki , Ichiro Shibuya , Shigeo Kawada , Akira Iino , Hiromasa Tanzaki , Kazuo Murano
发明人： Shigeru Yoshino , Junzo Ueki , Ichiro Shibuya , Shigeo Kawada , Akira Iino , Hiromasa Tanzaki , Kazuo Murano
CPC分类号： H05K13/0439 , Y10T29/4914 , Y10T29/53183
摘要： A simplified method of and apparatus for mounting an electronic part such as a transistor on a circuit board. Two or more selected ones of terminals of an electronic part are angularly bent in prior, and when the electronic part is to be mounted on a circuit board, the terminals are forcibly inserted into through-holes of the circuit board to an extent wherein bent portions of the terminals are projected below the circuit board. As a result, if a weak force is applied to pull the part off, the bent portions of the terminals will frictionally engage with edges of the through-holes to prevent the part from being removed from the board.
摘要翻译： 用于将诸如晶体管的电子部件安装在电路板上的简化方法和装置。 电子部件的两个以上选择的端子在以前成角度地弯曲，并且当电子部件被安装在电路板上时，端子被强制地插入到电路板的通孔中，其中弯曲部分 的端子投影在电路板的下方。 结果，如果施加弱力以拉出部件，则端子的弯曲部分将与通孔的边缘摩擦接合，以防止部件从板上移除。
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公开(公告)号：US20110223287A1
公开(公告)日：2011-09-15
申请号：US13128677
申请日：2009-10-30
CPC分类号： C12N1/16 , A23L2/56 , A23L23/00 , A23L27/22 , A23L33/145 , A23L33/175 , C12N1/18
摘要： Provided is a method for producing a yeast containing glutamic acid at a high concentration. In the method for culturing a yeast, a yeast in a stationary growth phase is subjected to liquid culture under the conditions that the pH of a liquid medium is 7.5 or higher and lower than 11. After the pH of the liquid medium for the yeast in a stationary growth phase is adjusted to 7.5 or higher and lower than 11, the yeast is further cultured, whereby a yeast with a high glutamic acid content can be produced. In the invention, as the yeast, Saccharomyces cerevisiae or Candida utilis can be used. Therefore, by using the yeast with a high glutamic acid content obtained by the above-said method and the extract obtained by extraction from the yeast, a seasoning composition and a food or drink with a high glutamic acid content can be provided.
摘要翻译： 本发明提供高浓度含有谷氨酸的酵母的制造方法。 在培养酵母的方法中，在液体培养基的pH为7.5以上且低于11的条件下，将静止生长期的酵母进行液体培养。酵母的液体培养基的pH 将固定生长期调整为7.5以上且低于11，进一步培养酵母，由此可以生产具有高谷氨酸含量的酵母。 在本发明中，作为酵母，可以使用酿酒酵母或产朊假丝酵母。 因此，通过使用通过上述方法获得的高谷氨酸含量的酵母和通过从酵母中提取获得的提取物，可以提供具有高谷氨酸含量的调味组合物和食品或饮料。
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公开(公告)号：US4857460A
公开(公告)日：1989-08-15
申请号：US808121
申请日：1985-12-12
申请人： Masamichi Takagi , Keiji Yano , Ichiro Shibuya , Minoru Morikawa
发明人： Masamichi Takagi , Keiji Yano , Ichiro Shibuya , Minoru Morikawa
CPC分类号： C12N15/81 , C07K14/39 , C12N15/65 , C12N15/815
摘要： A cycloheximide resistant gene of the present invention can be produced by cultivating yeast belonging to Candida genus and by cleaving a cycloheximide resistant gene from the yeast using restriction enzymes XbaI and Sau3AI.By incorporating the cycloheximide resistant gene together with the gene for useful substance into a plasmid, the transformant producing useful substance can be readily detected.
摘要翻译： 可以通过培养属于假丝酵母属的酵母并使用限制酶XbaI和Sau3AI从酵母中切割含有放线菌酮抗性的基因来制备本发明的放线菌酮抗性基因。 通过将放线菌酮抗性基因与有用物质的基因一起并入质粒中，可以容易地检测产生有用物质的转化体。
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公开(公告)号：US20110223288A1
公开(公告)日：2011-09-15
申请号：US13128683
申请日：2009-11-17
申请人： Ichiro Shibuya , Tetsuji Odani
发明人： Ichiro Shibuya , Tetsuji Odani
CPC分类号： C12N1/16 , A23L27/21 , A23L33/145 , A23L33/175
摘要： The present invention relates to a method for producing an alanine-rich yeast containing alanine at a high concentration and includes: a method involving liquid-culturing a yeast in a stationary phase of proliferation under the condition that the pH of a liquid medium is ≧7.5 and
摘要翻译： 本发明涉及含有高浓度丙氨酸的富含丙氨酸的酵母的制造方法，其特征在于，在液体介质的pH为≥7.5的条件下，包括在增殖的固定相中液体培养酵母的方法 和<11; 通过将液体培养基的pH调节至≥7.5和<11，然后在该pH范围内培养酵母来生产富含丙氨酸的酵母的方法; 一种生产富含丙氨酸的酵母的方法，其中酵母是酿酒酵母或产朊假丝酵母; 通过任何一种所述方法获得的富含丙氨酸的酵母; 从富含丙氨酸的酵母提取的富含丙氨酸的酵母提取物; 包含所述富含丙氨酸的酵母提取物的调味料组合物; 以及含有富含丙氨酸的酵母，所述富含丙氨酸的酵母提取物或所述调味组合物的含丙氨酸的食品和饮料。
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