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    Process for producing dipeptide,l-amino acid amide hydrolase to be used therein and process for producing l-amino acid amide hydrolase
    1.
    发明申请
    Process for producing dipeptide,l-amino acid amide hydrolase to be used therein and process for producing l-amino acid amide hydrolase 失效
    用于生产二肽，用于其中的1-氨基酰胺水解酶的方法和用于生产1-氨基酸酰胺水解酶的方法

    公开(公告)号：US20050054067A1

    公开(公告)日：2005-03-10

    申请号：US10481087

    申请日：2002-07-26

    申请人： Hiroyuki Nozaki ,  Ikuo Kira ,  Sonoko Suzuki ,  Kenzo Yokozeki

    发明人： Hiroyuki Nozaki ,  Ikuo Kira ,  Sonoko Suzuki ,  Kenzo Yokozeki

    IPC分类号： C07K5/062 ,  C07K5/06 ,  C12N1/19 ,  C12N1/21 ,  C12N9/00 ,  C12N9/48 ,  C12N9/52 ,  C12N9/80 ,  C12N15/57 ,  C12N15/63 ,  C12P21/00 ,  C12P21/02 ,  C12P21/06 ,  C12P1/02

    CPC分类号： C12P21/02 ,  C07K5/06026 ,  C12N9/48

    摘要： A process for industrially advantageously producing a dipeptide via a convenient pathway starting with less expensive and easily available materials is provided. A dipeptide is produced from an L-amino acid amide and an L-amino acid by using a culture of a microbe capable of synthesizing the dipeptide from the L-amino acid amide and the L-amino acid, microbial cells separated from the culture or a treated microbial cell product from the microbe. An L-amino acid amide hydrolase is obtained from a microbe belonging to the genus erwinia, genus Rhodococcus, genus Chryseobacterium, genus Micrococcus, genus Cryptococcus, genus Trichosporion, genus Rhodosporidium, genus Sporobolomyces, genus Tremela, genus Torulaspora, genus Sterigmatomyces or genus Rhodotorula. The hydrolase catalyzes a reaction that produces a dipeptide from an L-amino acid amide and an L-amino acid.

    摘要翻译： 提供了一种工业上有利地通过便宜的途径产生二肽的方法，从较便宜且容易获得的材料开始。 通过使用能够从L-氨基酸酰胺和L-氨基酸合成二肽的微生物培养物从L-氨基酸酰胺和L-氨基酸产生二肽，从培养物分离的微生物细胞或 来自微生物的经处理的微生物细胞产物。 L-氨基酸酰胺水解酶是从属于欧文氏菌属，红球菌属，巧克氏杆菌属，微球菌属，隐球菌属，三孢球菌属，红球菌属，孢子属，金龟子属，紫花a属，紫锥体属或红酵母属的微生物 。 该水解酶催化从L-氨基酸酰胺和L-氨基酸产生二肽的反应。

    Dipeptide production method, L-amino acid amide hydrolase used therein, and production method of L-amino acid amide hydrolase
    2.
    发明申请
    Dipeptide production method, L-amino acid amide hydrolase used therein, and production method of L-amino acid amide hydrolase 审中-公开
    二肽生产方法，其中使用的L-氨基酸酰胺水解酶，以及L-氨基酸酰胺水解酶的制备方法

    公开(公告)号：US20070042459A1

    公开(公告)日：2007-02-22

    申请号：US11249429

    申请日：2005-10-14

    申请人： Hiroyuki Nozaki ,  Ikuo Kira ,  Sonoko Suzuki ,  Kenzo Yokozeki

    发明人： Hiroyuki Nozaki ,  Ikuo Kira ,  Sonoko Suzuki ,  Kenzo Yokozeki

    IPC分类号： C12P21/06 ,  C12N9/48

    CPC分类号： C12P21/02 ,  C07K5/06026 ,  C12N9/48

    摘要： A process for industrially advantageously producing a dipeptide via a convenient pathway starting with less expensive and easily available materials is provided. A dipeptide is produced from an L-amino acid amide and an L-amino acid by using a culture of a microbe capable of synthesizing the dipeptide from the L-amino acid amide and the L-amino acid, microbial cells separated from the culture or a treated microbial cell product from the microbe. An L-amino acid amide hydrolase is obtained from a microbe belonging to the genus erwinia, genus Rhodococcus, genus Chryseobacterium, genus Micrococcus, genus Cryptococcus, genus Trichosporion, genus Rhodosporidium, genus Sporobolomyces, genus Tremela, genus Torulaspora, genus Sterigmatomyces or genus Rhodotorula. The hydrolase catalyzes a reaction that produces a dipeptide from an L-amino acid amide and an L-amino acid.

    摘要翻译： 提供了一种工业上有利地通过便宜的途径产生二肽的方法，从较便宜且容易获得的材料开始。 通过使用能够从L-氨基酸酰胺和L-氨基酸合成二肽的微生物培养物从L-氨基酸酰胺和L-氨基酸产生二肽，从培养物分离的微生物细胞或 来自微生物的经处理的微生物细胞产物。 L-氨基酸酰胺水解酶是从属于欧文氏菌属，红球菌属，巧克氏杆菌属，微球菌属，隐球菌属，三孢球菌属，红球菌属，孢子属，金龟子属，紫花a属，紫锥体属或红酵母属的微生物 。 该水解酶催化从L-氨基酸酰胺和L-氨基酸产生二肽的反应。

    Dipeptide production method, L-amino acid amide hydrolase used therein, and production method of L-amino acid amide hydrolase
    3.
    发明授权
    Dipeptide production method, L-amino acid amide hydrolase used therein, and production method of L-amino acid amide hydrolase 失效
    二肽生产方法，其中使用的L-氨基酸酰胺水解酶，以及L-氨基酸酰胺水解酶的制备方法

    公开(公告)号：US07037673B2

    公开(公告)日：2006-05-02

    申请号：US10481087

    申请日：2002-07-26

    申请人： Hiroyuki Nozaki ,  Ikuo Kira ,  Sonoko Suzuki ,  Kenzo Yokozeki

    发明人： Hiroyuki Nozaki ,  Ikuo Kira ,  Sonoko Suzuki ,  Kenzo Yokozeki

    IPC分类号： C12P21/00 ,  C12N9/58

    CPC分类号： C12P21/02 ,  C07K5/06026 ,  C12N9/48

    摘要： A process for industrially advantageously producing a dipeptide via a convenient pathway starting with less expensive and easily available materials is provided. A dipeptide is produced from an L-amino acid amide and an L-amino acid by using a culture of a microbe capable of synthesizing the dipeptide from the L-amino acid amide and the L-amino acid, microbial cells separated from the culture or a treated microbial cell product from the microbe. An L-amino acid amide hydrolase is obtained from a microbe belonging to the genus erwinia, genus Rhodococcus, genus Chryseobacterium, genus Micrococcus, genus Cryptococcus, genus Trichosporion, genus Rhodosporidium, genus Sporobolomyces, genus Tremela, genus Torulaspora, genus Sterigmatomyces or genus Rhodotorula. The hydrolase catalyzes a reaction that produces a dipeptide from an L-amino acid amide and an L-amino acid.

    摘要翻译： 提供了一种工业上有利地通过便宜的途径产生二肽的方法，从较便宜且容易获得的材料开始。 通过使用能够从L-氨基酸酰胺和L-氨基酸合成二肽的微生物培养物从L-氨基酸酰胺和L-氨基酸产生二肽，从培养物分离的微生物细胞或 来自微生物的经处理的微生物细胞产物。 L-氨基酸酰胺水解酶是从属于欧文氏菌属，红球菌属，巧克氏杆菌属，微球菌属，隐球菌属，三孢球菌属，红球菌属，孢子属，金龟子属，紫花a属，紫锥体属或红酵母属的微生物 。 该水解酶催化从L-氨基酸酰胺和L-氨基酸产生二肽的反应。

    Mutant microorganism and method for producing peptide using the same
    4.
    发明申请
    Mutant microorganism and method for producing peptide using the same 审中-公开
    突变体微生物及其使用方法

    公开(公告)号：US20050176150A1

    公开(公告)日：2005-08-11

    申请号：US11050829

    申请日：2005-02-07

    申请人： Ikuo Kira ,  Kenzo Yokozeki ,  Sonoko Suzuki ,  Yasuhiro Mihara ,  Yoshinori Hirao

    发明人： Ikuo Kira ,  Kenzo Yokozeki ,  Sonoko Suzuki ,  Yasuhiro Mihara ,  Yoshinori Hirao

    IPC分类号： C12N1/21 ,  C12N9/88 ,  C12N15/74

    CPC分类号： C12N9/88 ,  C12N15/74

    摘要： The present invention provides a method for producing the peptides comprising: cultivating in medium the microorganisms wherein at least one gene selected from the group consisting of a gene encoding; aminoacylhistidine peptidase; a gene encoding leucylaminopeptidase; and a gene encoding isoaspartyldipeptidase, respectively; has been disrupted on the chromosome and wherein preferably transformed with the recombinant DNA, comprising polynucleotide encoding the proteins having peptide-synthesizing activity, mixing at least one of the cultivated microorganisms and the disrupted cells of the microorganisms with the carboxy and amine components for the peptide synthesis.

    摘要翻译： 本发明提供了一种生产肽的方法，包括：在培养基中培养微生物，其中至少一种选自下组的基因：基因编码; 氨酰基组氨酸肽酶; 编码亮氨酰氨基肽酶的基因; 和分别编码异天竺葵酰二肽酶的基因; 已经在染色体上被破坏，并且其中优选用重组DNA转化，包括编码具有肽合成活性的蛋白质的多核苷酸，将至少一种培养的微生物和微生物的破坏细胞与肽的羧基和胺组分混合 合成。