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公开(公告)号：US20120058511A1
公开(公告)日：2012-03-08
申请号：US12828772
申请日：2010-07-01
申请人： Ikuo KIRA , Kenzo Yokozeki , Sonoko Suzuki , Yasuhiro Mihara , Yoshinori Hirao
发明人： Ikuo KIRA , Kenzo Yokozeki , Sonoko Suzuki , Yasuhiro Mihara , Yoshinori Hirao
摘要： The present invention provides a method for producing the peptides comprising: cultivating in medium the microorganisms wherein at least one gene selected from the group consisting of a gene encoding; aminoacylhistidine peptidase; a gene encoding leucylaminopeptidase; and a gene encoding isoaspartyldipeptidase, respectively; has been disrupted on the chromosome and wherein preferably transformed with the recombinant DNA, comprising polynucleotide encoding the proteins having peptide-synthesizing activity, mixing at least one of the cultivated microorganisms and the disrupted cells of the microorganisms with the carboxy and amine components for the peptide synthesis.
摘要翻译： 本发明提供了一种生产肽的方法，包括：在培养基中培养微生物，其中至少一种选自下组的基因：基因编码; 氨酰基组氨酸肽酶; 编码亮氨酰氨基肽酶的基因; 和分别编码异天竺葵酰二肽酶的基因; 已经在染色体上被破坏，并且其中优选用重组DNA转化，包括编码具有肽合成活性的蛋白质的多核苷酸，将至少一种培养的微生物和微生物的破坏细胞与肽的羧基和胺组分混合 合成。
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公开(公告)号：US20050176150A1
公开(公告)日：2005-08-11
申请号：US11050829
申请日：2005-02-07
申请人： Ikuo Kira , Kenzo Yokozeki , Sonoko Suzuki , Yasuhiro Mihara , Yoshinori Hirao
发明人： Ikuo Kira , Kenzo Yokozeki , Sonoko Suzuki , Yasuhiro Mihara , Yoshinori Hirao
摘要： The present invention provides a method for producing the peptides comprising: cultivating in medium the microorganisms wherein at least one gene selected from the group consisting of a gene encoding; aminoacylhistidine peptidase; a gene encoding leucylaminopeptidase; and a gene encoding isoaspartyldipeptidase, respectively; has been disrupted on the chromosome and wherein preferably transformed with the recombinant DNA, comprising polynucleotide encoding the proteins having peptide-synthesizing activity, mixing at least one of the cultivated microorganisms and the disrupted cells of the microorganisms with the carboxy and amine components for the peptide synthesis.
摘要翻译： 本发明提供了一种生产肽的方法，包括：在培养基中培养微生物，其中至少一种选自下组的基因：基因编码; 氨酰基组氨酸肽酶; 编码亮氨酰氨基肽酶的基因; 和分别编码异天竺葵酰二肽酶的基因; 已经在染色体上被破坏，并且其中优选用重组DNA转化，包括编码具有肽合成活性的蛋白质的多核苷酸，将至少一种培养的微生物和微生物的破坏细胞与肽的羧基和胺组分混合 合成。
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3.发明申请Process for producing dipeptide,l-amino acid amide hydrolase to be used therein and process for producing l-amino acid amide hydrolase 失效用于生产二肽，用于其中的1-氨基酰胺水解酶的方法和用于生产1-氨基酸酰胺水解酶的方法公开(公告)号：US20050054067A1
公开(公告)日：2005-03-10
申请号：US10481087
申请日：2002-07-26
申请人： Hiroyuki Nozaki , Ikuo Kira , Sonoko Suzuki , Kenzo Yokozeki
发明人： Hiroyuki Nozaki , Ikuo Kira , Sonoko Suzuki , Kenzo Yokozeki
IPC分类号： C07K5/062 , C07K5/06 , C12N1/19 , C12N1/21 , C12N9/00 , C12N9/48 , C12N9/52 , C12N9/80 , C12N15/57 , C12N15/63 , C12P21/00 , C12P21/02 , C12P21/06 , C12P1/02
CPC分类号： C12P21/02 , C07K5/06026 , C12N9/48
摘要： A process for industrially advantageously producing a dipeptide via a convenient pathway starting with less expensive and easily available materials is provided. A dipeptide is produced from an L-amino acid amide and an L-amino acid by using a culture of a microbe capable of synthesizing the dipeptide from the L-amino acid amide and the L-amino acid, microbial cells separated from the culture or a treated microbial cell product from the microbe. An L-amino acid amide hydrolase is obtained from a microbe belonging to the genus erwinia, genus Rhodococcus, genus Chryseobacterium, genus Micrococcus, genus Cryptococcus, genus Trichosporion, genus Rhodosporidium, genus Sporobolomyces, genus Tremela, genus Torulaspora, genus Sterigmatomyces or genus Rhodotorula. The hydrolase catalyzes a reaction that produces a dipeptide from an L-amino acid amide and an L-amino acid.
摘要翻译： 提供了一种工业上有利地通过便宜的途径产生二肽的方法，从较便宜且容易获得的材料开始。 通过使用能够从L-氨基酸酰胺和L-氨基酸合成二肽的微生物培养物从L-氨基酸酰胺和L-氨基酸产生二肽，从培养物分离的微生物细胞或 来自微生物的经处理的微生物细胞产物。 L-氨基酸酰胺水解酶是从属于欧文氏菌属，红球菌属，巧克氏杆菌属，微球菌属，隐球菌属，三孢球菌属，红球菌属，孢子属，金龟子属，紫花a属，紫锥体属或红酵母属的微生物 。 该水解酶催化从L-氨基酸酰胺和L-氨基酸产生二肽的反应。
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4.发明申请Dipeptide production method, L-amino acid amide hydrolase used therein, and production method of L-amino acid amide hydrolase 审中-公开二肽生产方法，其中使用的L-氨基酸酰胺水解酶，以及L-氨基酸酰胺水解酶的制备方法公开(公告)号：US20070042459A1
公开(公告)日：2007-02-22
申请号：US11249429
申请日：2005-10-14
申请人： Hiroyuki Nozaki , Ikuo Kira , Sonoko Suzuki , Kenzo Yokozeki
发明人： Hiroyuki Nozaki , Ikuo Kira , Sonoko Suzuki , Kenzo Yokozeki
CPC分类号： C12P21/02 , C07K5/06026 , C12N9/48
摘要： A process for industrially advantageously producing a dipeptide via a convenient pathway starting with less expensive and easily available materials is provided. A dipeptide is produced from an L-amino acid amide and an L-amino acid by using a culture of a microbe capable of synthesizing the dipeptide from the L-amino acid amide and the L-amino acid, microbial cells separated from the culture or a treated microbial cell product from the microbe. An L-amino acid amide hydrolase is obtained from a microbe belonging to the genus erwinia, genus Rhodococcus, genus Chryseobacterium, genus Micrococcus, genus Cryptococcus, genus Trichosporion, genus Rhodosporidium, genus Sporobolomyces, genus Tremela, genus Torulaspora, genus Sterigmatomyces or genus Rhodotorula. The hydrolase catalyzes a reaction that produces a dipeptide from an L-amino acid amide and an L-amino acid.
摘要翻译： 提供了一种工业上有利地通过便宜的途径产生二肽的方法，从较便宜且容易获得的材料开始。 通过使用能够从L-氨基酸酰胺和L-氨基酸合成二肽的微生物培养物从L-氨基酸酰胺和L-氨基酸产生二肽，从培养物分离的微生物细胞或 来自微生物的经处理的微生物细胞产物。 L-氨基酸酰胺水解酶是从属于欧文氏菌属，红球菌属，巧克氏杆菌属，微球菌属，隐球菌属，三孢球菌属，红球菌属，孢子属，金龟子属，紫花a属，紫锥体属或红酵母属的微生物 。 该水解酶催化从L-氨基酸酰胺和L-氨基酸产生二肽的反应。
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5.发明授权Dipeptide production method, L-amino acid amide hydrolase used therein, and production method of L-amino acid amide hydrolase 失效二肽生产方法，其中使用的L-氨基酸酰胺水解酶，以及L-氨基酸酰胺水解酶的制备方法公开(公告)号：US07037673B2
公开(公告)日：2006-05-02
申请号：US10481087
申请日：2002-07-26
申请人： Hiroyuki Nozaki , Ikuo Kira , Sonoko Suzuki , Kenzo Yokozeki
发明人： Hiroyuki Nozaki , Ikuo Kira , Sonoko Suzuki , Kenzo Yokozeki
CPC分类号： C12P21/02 , C07K5/06026 , C12N9/48
摘要： A process for industrially advantageously producing a dipeptide via a convenient pathway starting with less expensive and easily available materials is provided. A dipeptide is produced from an L-amino acid amide and an L-amino acid by using a culture of a microbe capable of synthesizing the dipeptide from the L-amino acid amide and the L-amino acid, microbial cells separated from the culture or a treated microbial cell product from the microbe. An L-amino acid amide hydrolase is obtained from a microbe belonging to the genus erwinia, genus Rhodococcus, genus Chryseobacterium, genus Micrococcus, genus Cryptococcus, genus Trichosporion, genus Rhodosporidium, genus Sporobolomyces, genus Tremela, genus Torulaspora, genus Sterigmatomyces or genus Rhodotorula. The hydrolase catalyzes a reaction that produces a dipeptide from an L-amino acid amide and an L-amino acid.
摘要翻译： 提供了一种工业上有利地通过便宜的途径产生二肽的方法，从较便宜且容易获得的材料开始。 通过使用能够从L-氨基酸酰胺和L-氨基酸合成二肽的微生物培养物从L-氨基酸酰胺和L-氨基酸产生二肽，从培养物分离的微生物细胞或 来自微生物的经处理的微生物细胞产物。 L-氨基酸酰胺水解酶是从属于欧文氏菌属，红球菌属，巧克氏杆菌属，微球菌属，隐球菌属，三孢球菌属，红球菌属，孢子属，金龟子属，紫花a属，紫锥体属或红酵母属的微生物 。 该水解酶催化从L-氨基酸酰胺和L-氨基酸产生二肽的反应。
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6.发明授权公开(公告)号：US07749742B2
公开(公告)日：2010-07-06
申请号：US11940555
申请日：2007-11-15
申请人： Kenzo Yokozeki , Sonoko Suzuki , Seiichi Hara , Isao Abe
发明人： Kenzo Yokozeki , Sonoko Suzuki , Seiichi Hara , Isao Abe
摘要： A method is disclosed that allows the production of peptides having three or more amino acid residues easily, inexpensively and at high yield without going through a complex synthesis method. A novel enzyme that efficiently produces a peptide from bacteria belonging to the genus Empedobacter or the genus Sphingobacterium is provided. The enzyme acts on a carboxy component and an amine component to form peptides having three or more amino acid residues by acting on a carboxy component and an amine component.
摘要翻译： 公开了一种能够容易地，低成本地以高产率制备具有三个或更多个氨基酸残基的肽而不经过复合合成方法的方法。 提供了一种新颖的酶，其有效地从属于Empedobacter属或Sphingobacterium属的细菌产生肽。 该酶作用于羧基组分和胺组分以通过作用于羧基组分和胺组分形成具有三个或更多个氨基酸残基的肽。
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公开(公告)号：US20070048838A1
公开(公告)日：2007-03-01
申请号：US11589842
申请日：2006-10-31
CPC分类号： C12P21/02 , C07K5/06026 , C12N9/48
摘要： The present invention provides a method for producing dipeptide using inexpensively acquirable starting materials by an industrially advantageous and simple pathway. Dipeptide is produced from L-amino acid ester and L-amino acid using a culture of microbes having the ability to produce a dipeptide from an L-amino acid ester and an L-amino acid, using microbial cells isolated from the culture, or a treated microbial cell product of the microbe.
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8.发明授权Peptide-forming enzyme gene, peptide-forming enzyme, and peptide producing method 失效肽形成酶基因，肽形成酶和肽生产方法公开(公告)号：US07618796B2
公开(公告)日：2009-11-17
申请号：US11589842
申请日：2006-10-31
IPC分类号： C12P21/02
CPC分类号： C12P21/02 , C07K5/06026 , C12N9/48
摘要： The present invention provides a method for producing dipeptide using inexpensively acquirable starting materials by an industrially advantageous and simple pathway. Dipeptide is produced from L-amino acid ester and L-amino acid using a culture of microbes having the ability to produce a dipeptide from an L-amino acid ester and an L-amino acid, using microbial cells isolated from the culture, or a treated microbial cell product of the microbe.
摘要翻译： 本发明提供了通过工业上有利且简单的途径使用廉价可获得的原料生产二肽的方法。 使用从培养物分离的微生物细胞，使用从L-氨基酸酯和L-氨基酸产生二肽的微生物培养物，由L-氨基酸酯和L-氨基酸产生二肽，或 处理微生物细菌产物。
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公开(公告)号：US07754466B2
公开(公告)日：2010-07-13
申请号：US10476885
申请日：2002-07-26
申请人： Kenzo Yokozeki , Sonoko Suzuki
发明人： Kenzo Yokozeki , Sonoko Suzuki
CPC分类号： C12P21/02 , C07K5/06026 , C12N9/48
摘要： The present invention provides a method of producing a dipeptide from starting materials that are available at low costs through a route industrially advantageous and simple. Dipeptides are produced from amino acid esters and amino acids by using a culture of a microbe having an ability to produce a dipeptide from an amino acid ester and an amino acid, microbial cells separated from the culture, or treated microbial cell product.
摘要翻译： 本发明提供了一种通过工业上有利和简单的途径以低成本获得的原料制备二肽的方法。 通过使用具有从氨基酸酯和氨基酸产生二肽的能力的微生物培养物，从培养物分离的微生物细胞或经处理的微生物细胞产物，由氨基酸酯和氨基酸产生二肽。
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10.发明授权公开(公告)号：US07338780B2
公开(公告)日：2008-03-04
申请号：US10849814
申请日：2004-05-21
申请人： Kenzo Yokozeki , Sonoko Suzuki , Seiichi Hara , Isao Abe
发明人： Kenzo Yokozeki , Sonoko Suzuki , Seiichi Hara , Isao Abe
摘要： A method is disclosed that allows the production of peptides having three or more amino acid residues easily, inexpensively and at high yield without going through a complex synthesis method. A novel enzyme that efficiently produces a peptide from bacteria belonging to the genus Empedobacter or the genus Sphingobacterium is provided. The enzyme acts on a carboxy component and an amine component to form peptides having three or more amino acid residues by acting on a carboxy component and an amine component.
摘要翻译： 公开了一种能够容易地，低成本地以高产率制备具有三个或更多个氨基酸残基的肽而不经过复合合成方法的方法。 提供了一种新颖的酶，其有效地从属于Empedobacter属或Sphingobacterium属的细菌产生肽。 该酶作用于羧基组分和胺组分以通过作用于羧基组分和胺组分形成具有三个或更多个氨基酸残基的肽。
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