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33 条记录 (耗时 0.051 秒)

    Diminishing viral gene expression by promoter replacement
    1.
    发明授权
    Diminishing viral gene expression by promoter replacement 失效
    通过启动子置换减少病毒基因表达

    公开(公告)号：US07244617B2

    公开(公告)日：2007-07-17

    申请号：US10677727

    申请日：2003-10-02

    申请人： Bingliang Fang ,  Jack A. Roth

    发明人： Bingliang Fang ,  Jack A. Roth

    IPC分类号： C12N15/861 ,  C12N15/12 ,  C12N15/34

    CPC分类号： C12N7/00 ,  A61K38/00 ,  A61K48/00 ,  C07K14/005 ,  C07K14/39 ,  C07K14/475 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/002 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2830/702

    摘要： The present invention provides viral vectors that have been engineered to contain a synthetic promoter that controls at least one essential gene. The synthetic promoter is induced by a specific gene product not normally produced in the cells in which the viral vector is to be transferred. The vectors are propagated in producer or helper cells that express the inducing factor, thereby permitting the virus to replicate to high titer. The lack of the inducing factor in the target cells precludes viral replication, however, meaning that no vector toxicity or immunogenicity arises. Where the virus carries a gene of interest, this should provide for higher level expression for longer periods of time than with current vectors. Methods for making the vectors, helper cells, and their use in protein production, vaccines and gene therapy are disclosed.

    摘要翻译： 本发明提供已被工程化以含有控制至少一种必需基因的合成启动子的病毒载体。 合成启动子由病毒载体转移的细胞中通常不产生的特异性基因产物诱导。 载体在表达诱导因子的生产者或辅助细胞中繁殖，从而允许病毒复制至高滴度。 靶细胞缺乏诱导因子排除了病毒复制，但是意味着不会引起载体毒性或免疫原性。 当病毒携带感兴趣的基因时，这应该提供比当前载体更长的时间段的更高级别的表达。 公开了制备载体，辅助细胞及其在蛋白质生产，疫苗和基因治疗中的用途的方法。

    p16 expression constructs and their application in cancer therapy
    2.
    发明授权
    p16 expression constructs and their application in cancer therapy 失效
    p16表达构建体及其在癌症治疗中的应用

    公开(公告)号：US07163925B1

    公开(公告)日：2007-01-16

    申请号：US09080935

    申请日：1998-05-19

    申请人： Xiaomei Jin ,  Jack A. Roth

    发明人： Xiaomei Jin ,  Jack A. Roth

    IPC分类号： A61K48/00 ,  C12N15/63 ,  C12N15/86 ,  C12N15/861

    CPC分类号： C12N15/86 ,  A61K48/00 ,  A61K48/005 ,  C12N2710/10343

    摘要： A variety of genetic constructs are disclosed that will find both in vitro and in vivo use in the area of tumor biology and cancer therapy. In particular, expression constructs are provided that contain a p16 encoding region and other regulatory elements necessary for the expression of a p16 transcript. One version of the expression construct is a replication-deficient adenoviral vector. Also provided are methods for the transformation of cell lines and the inhibition of cancer cell proliferation.

    摘要翻译： 公开了在肿瘤生物学和癌症治疗领域中体外和体内使用的多种遗传构建体。 特别地，提供了含有p16编码区和表达p16转录物所必需的其它调节元件的表达构建体。 表达构建体的一个版本是复制缺陷型腺病毒载体。 还提供了转化细胞系和抑制癌细胞增殖的方法。

    Recombinant P53 adenovirus methods and compositions
    4.
    发明授权
    Recombinant P53 adenovirus methods and compositions 失效
    重组P53腺病毒方法和组合物

    公开(公告)号：US07033750B2

    公开(公告)日：2006-04-25

    申请号：US10170240

    申请日：2002-06-11

    申请人： Wei-Wei Zhang ,  Jack A Roth

    发明人： Wei-Wei Zhang ,  Jack A Roth

    IPC分类号： C12N15/861 ,  C12N7/01 ,  C12N7/02 ,  C12Q1/70

    CPC分类号： C12N15/86 ,  A61K38/1709 ,  A61K48/00 ,  C07K14/4746 ,  C12N7/00 ,  C12N2710/10343 ,  A61K2300/00

    摘要： Described are simplified and efficient methods for preparing recombinant adenovirus using liposome-mediated cotransfection and the direct observation of a cytopathic effect (CPE) in the transfected cells. Also disclosed are compositions and methods involving novel p53 adenovirus constructs, including methods for restoring p53 function and tumor suppression in cells and animals having abnormal p53.

    摘要翻译： 描述了使用脂质体介导的共转染和直接观察转染细胞中细胞病变效应（CPE）的方法制备重组腺病毒的简化和有效的方法。 还公开了涉及新型p53腺病毒构建体的组合物和方法，包括在具有异常p53的细胞和动物中恢复p53功能和肿瘤抑制的方法。

    Methods for the administration of adenovirus p53
    6.
    发明授权
    Methods for the administration of adenovirus p53 失效
    腺病毒p53的施用方法

    公开(公告)号：US06805858B1

    公开(公告)日：2004-10-19

    申请号：US09413109

    申请日：1999-10-06

    申请人： Wei-Wei Zhang ,  Jack A. Roth

    发明人： Wei-Wei Zhang ,  Jack A. Roth

    IPC分类号： A61K4800

    CPC分类号： C12N15/86 ,  A61K38/00 ,  A61K38/1709 ,  A61K48/00 ,  C07K14/4746 ,  C12N2710/10343 ,  A61K2300/00

    摘要： Described are simplified and efficient methods for preparing recombinant adenovirus using liposome-mediated cotransfection and the direct observation of a cytopathic effect (CPE) in the transfected cells. Also disclosed are compositions and methods involving novel p53 adenovirus constructs, including methods for restoring p53 function and tumor suppression in cells and animals having abnormal p53.

    摘要翻译： 描述了使用脂质体介导的共转染和直接观察转染细胞中细胞病变效应（CPE）的方法制备重组腺病毒的简化和有效的方法。 还公开了涉及新型p53腺病毒构建体的组合物和方法，包括在具有异常p53的细胞和动物中恢复p53功能和肿瘤抑制的方法。

    Recombinant P53 adenovirus methods and compositions
    7.
    发明授权
    Recombinant P53 adenovirus methods and compositions 失效
    重组P53腺病毒方法和组合物

    公开(公告)号：US06740320B1

    公开(公告)日：2004-05-25

    申请号：US08459713

    申请日：1995-06-02

    申请人： Wei-Wei Zhang ,  Jack A. Roth

    发明人： Wei-Wei Zhang ,  Jack A. Roth

    IPC分类号： A61K4800

    CPC分类号： C12N15/86 ,  A61K38/1709 ,  A61K48/00 ,  C07K14/4746 ,  C12N7/00 ,  C12N2710/10343 ,  A61K2300/00

    摘要： Described are simplified and efficient methods for preparing recombinant adenovirus using liposome-mediated cotransfection and the direct observation of a cytopathic effect (CPE) in the transfected cells. Also disclosed are compositions and methods involving novel p53 adenovirus constructs, including methods for restoring p53 function and tumor suppression in cells and animals having abnormal p53.

    摘要翻译： 描述了使用脂质体介导的共转染和直接观察转染细胞中细胞病变效应（CPE）的方法制备重组腺病毒的简化和有效的方法。 还公开了涉及新型p53腺病毒构建体的组合物和方法，包括在具有异常p53的细胞和动物中恢复p53功能和肿瘤抑制的方法。

    Diminishing viral gene expression by promoter replacement
    8.
    发明授权
    Diminishing viral gene expression by promoter replacement 失效
    通过启动子置换减少病毒基因表达

    公开(公告)号：US06630344B1

    公开(公告)日：2003-10-07

    申请号：US09650946

    申请日：2000-08-29

    申请人： Bingliang Fang ,  Jack A. Roth

    发明人： Bingliang Fang ,  Jack A. Roth

    IPC分类号： C12N15861

    CPC分类号： C12N7/00 ,  A61K38/00 ,  A61K48/00 ,  C07K14/005 ,  C07K14/39 ,  C07K14/475 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/002 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2830/702

    摘要： The present invention provides viral vectors that have been engineered to contain a synthetic promoter that controls at least one essential gene. The synthetic promoter is induced by a specific gene product not normally produced in the cells in which the viral vector is to be transferred. The vectors are propagated in producer or helper cells that express the inducing factor, thereby permitting the virus to replicate to high titer. The lack of the inducing factor in the target cells precludes viral replication, however, meaning that no vector toxicity or immunogenicity arises. Where the virus carries a gene of interest, this should provide for higher level expression for longer periods of time than with current vectors. Methods for making the vectors, helper cells, and their use in protein production, vaccines and gene therapy are disclosed.

    摘要翻译： 本发明提供已被工程化以含有控制至少一种必需基因的合成启动子的病毒载体。 合成启动子由病毒载体转移的细胞中通常不产生的特异性基因产物诱导。 载体在表达诱导因子的生产者或辅助细胞中繁殖，从而允许病毒复制至高滴度。 靶细胞中缺乏诱导因子排除病毒复制，但是意味着不会引起载体毒性或免疫原性。 当病毒携带感兴趣的基因时，这应该提供比当前载体更长的时间段的更高级别的表达。 公开了制备载体，辅助细胞及其在蛋白质生产，疫苗和基因治疗中的用途的方法。