利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

28 条记录 (耗时 0.033 秒)

    P16 expression constructs and their application in cancer therapy
    1.
    发明授权
    P16 expression constructs and their application in cancer therapy 失效
    P16表达构建及其在癌症治疗中的应用

    公开(公告)号：US06251871B1

    公开(公告)日：2001-06-26

    申请号：US08910722

    申请日：1997-08-13

    申请人： Xiaomei Jin ,  Jack A. Roth

    发明人： Xiaomei Jin ,  Jack A. Roth

    IPC分类号： A61K4800

    CPC分类号： C07K14/47 ,  A61K38/00 ,  A61K48/00 ,  C12N2799/022

    摘要： A variety of genetic constructs are disclosed that will find both in vitro and in vivo use in the area of tumor biology and cancer therapy. In particular, expression constructs are provided that contain a p16 encoding region and other regulatory elements necessary for the expression of a p16 transcript. One version of the expression construct is a replication-deficient adenoviral vector. Also provided are methods for the transformation of cell lines and the inhibition of cancer cell proliferation.

    摘要翻译： 公开了在肿瘤生物学和癌症治疗领域中体外和体内使用的多种遗传构建体。 特别地，提供了含有p16编码区和表达p16转录物所必需的其它调节元件的表达构建体。 表达构建体的一个版本是复制缺陷型腺病毒载体。 还提供了转化细胞系和抑制癌细胞增殖的方法。

    p16 expression constructs and their application in cancer therapy
    2.
    发明授权
    p16 expression constructs and their application in cancer therapy 失效
    p16表达构建体及其在癌症治疗中的应用

    公开(公告)号：US07163925B1

    公开(公告)日：2007-01-16

    申请号：US09080935

    申请日：1998-05-19

    申请人： Xiaomei Jin ,  Jack A. Roth

    发明人： Xiaomei Jin ,  Jack A. Roth

    IPC分类号： A61K48/00 ,  C12N15/63 ,  C12N15/86 ,  C12N15/861

    CPC分类号： C12N15/86 ,  A61K48/00 ,  A61K48/005 ,  C12N2710/10343

    摘要： A variety of genetic constructs are disclosed that will find both in vitro and in vivo use in the area of tumor biology and cancer therapy. In particular, expression constructs are provided that contain a p16 encoding region and other regulatory elements necessary for the expression of a p16 transcript. One version of the expression construct is a replication-deficient adenoviral vector. Also provided are methods for the transformation of cell lines and the inhibition of cancer cell proliferation.

    摘要翻译： 公开了在肿瘤生物学和癌症治疗领域中体外和体内使用的多种遗传构建体。 特别地，提供了含有p16编码区和表达p16转录物所必需的其它调节元件的表达构建体。 表达构建体的一个版本是复制缺陷型腺病毒载体。 还提供了转化细胞系和抑制癌细胞增殖的方法。

    p16 expression constructs and their application in cancer therapy
    3.
    发明授权
    p16 expression constructs and their application in cancer therapy 失效
    p16表达构建体及其在癌症治疗中的应用

    公开(公告)号：US5691198A

    公开(公告)日：1997-11-25

    申请号：US502881

    申请日：1995-07-17

    申请人： Xiaomei Jin ,  Jack A. Roth

    发明人： Xiaomei Jin ,  Jack A. Roth

    IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K48/00 ,  A61P35/00 ,  C07K14/47 ,  C12N5/10 ,  C12N15/00 ,  C12Q1/68 ,  C12R1/92 ,  C12N5/00

    CPC分类号： C07K14/47 ,  A61K38/00 ,  A61K48/00 ,  C12N2799/022

    摘要： A variety of genetic constructs are disclosed that will find both in vitro and in vivo use in the area of tumor biology and cancer therapy. In particular, expression constructs are provided that contain a p16 encoding region and other regulatory elements necessary for the expression of a p16 transcript. One version of the expression construct is a replication-deficient adenoviral vector. Also provided are methods for the transformation of cell lines and the inhibition of cancer cell proliferation.

    摘要翻译： 公开了在肿瘤生物学和癌症治疗领域中体外和体内使用的多种遗传构建体。 特别地，提供了含有p16编码区和表达p16转录物所必需的其它调节元件的表达构建体。 表达构建体的一个版本是复制缺陷型腺病毒载体。 还提供了转化细胞系和抑制癌细胞增殖的方法。

    Anti-sense p21 k-ras
    7.
    发明授权
    Anti-sense p21 k-ras 失效
    反感p21 k-ras

    公开(公告)号：US06436700B1

    公开(公告)日：2002-08-20

    申请号：US07987235

    申请日：1992-12-07

    申请人： Jack A. Roth ,  Tapas Mukopadhyay ,  Michael Tainsky

    发明人： Jack A. Roth ,  Tapas Mukopadhyay ,  Michael Tainsky

    IPC分类号： C12N1500

    CPC分类号： C12N15/1135 ,  A61K38/1709 ,  A61K48/00 ,  C07K14/82 ,  A61K2300/00

    摘要： Disclosed are methods and compositions for the selective inhibition gene expression through the application of antisense RNA technology. Antisense RNA constructs of the present invention employ the use of antisense intron DNA corresponding to distinct intron regions of the gene whose expression is targeted for down-regulation. In an exemplary embodiment, a human lung cancer cell line (NCI-H460a) with a homozygous spontaneous K-ras mutation was transfected with a recombinant plasmid that synthesizes a genomic segment of K-ras in antisense orientation. Translation of the mutated K-ras mRNA was specifically inhibited, whereas expression of H-ras and N-ras was unchanged. A three-fold growth inhibition occurred in H460a cells when expression of the mutated ras p21 protein was down-regulated by antisense RNA and cells remained viable. The growth of H460a tumors in nu/nu mice was substantially reduced by expressed K-ras antisense RNA.

    摘要翻译： 公开了通过应用反义RNA技术选择性抑制基因表达的方法和组合物。 本发明的反义RNA构建体使用对应于其表达靶向于下调的基因的不同内含子区域的反义内含子DNA。 在一个示例性实施方案中，用重组质粒转染具有纯合自发K-ras突变的人肺癌细胞系（NCI-H460a），其以反义方向合成K-ras基因组片段。 突变的K-ras mRNA的翻译被特异性抑制，而H-ras和N-ras的表达不变。 当突变的ras p21蛋白的表达被反义RNA下调时，H460a细胞发生三倍的生长抑制，细胞仍然存活。 通过表达的K-ras反义RNA，nu / nu小鼠中H460a肿瘤的生长显着降低。

    Antihelminthic drugs as a treatment for hyperproliferative diseases
    10.
    发明授权
    Antihelminthic drugs as a treatment for hyperproliferative diseases 失效
    抗血栓药物作为过度增殖性疾病的治疗

    公开(公告)号：US07423015B1

    公开(公告)日：2008-09-09

    申请号：US10043877

    申请日：2002-01-09

    申请人： Tapas Mukhopadhyay ,  Sunil Chada ,  Abner Mhashilkar ,  Jack A. Roth

    发明人： Tapas Mukhopadhyay ,  Sunil Chada ,  Abner Mhashilkar ,  Jack A. Roth

    IPC分类号： A01K43/78

    CPC分类号： A61K31/4184 ,  A61K31/4439

    摘要： The present invention is directed to the use of benzimidazole derivatives for the treatment of tumors and in combination with tumor suppressor gene therapy. In a particular embodiment, treatment of p53-positive tumors with benzimidazole derivatives induces p53 expression and increases its half-life, resulting in apoptotic death of the tumor cells. Similarly, in conjunction with p53 gene therapy, benzimidazole derivatives induce p53 expression and accumulation in tumor cells regardless of their p53 status. The combination treatment subsequently elicits apoptosis of the tumor cells.

    摘要翻译： 本发明涉及苯并咪唑衍生物用于治疗肿瘤并与肿瘤抑制基因治疗相结合的用途。 在一个具体实施方案中，用苯并咪唑衍生物治疗p53阳性肿瘤诱导p53表达并增加其半衰期，导致肿瘤细胞的凋亡死亡。 类似地，结合p53基因治疗，苯并咪唑衍生物诱导肿瘤细胞中的p53表达和积累，而不管其p53状态如何。 随后的联合治疗引起肿瘤细胞凋亡。