公开(公告)号：US10392578B2

公开(公告)日：2019-08-27

申请号：US13151014

申请日：2011-06-01

Applicant: Stephen Robert Cherinko ,  Robert Cody Kertis ,  Kirk E. Apt ,  Paul Warren Behrens ,  Jon Milton Hansen ,  Joseph W. Pfeifer, III ,  Tracey Lynn Stahl ,  Ross Zirkle ,  Nasrin Tabayeh Burke ,  Krishna Raman ,  Neil Francis Leininger

Inventor： Stephen Robert Cherinko ,  Robert Cody Kertis ,  Kirk E. Apt ,  Paul Warren Behrens ,  Jon Milton Hansen ,  Joseph W. Pfeifer, III ,  Tracey Lynn Stahl ,  Ross Zirkle ,  Nasrin Tabayeh Burke ,  Krishna Raman ,  Neil Francis Leininger

IPC: C11B1/10 ,  C11B3/02 ,  C11B3/16 ,  C12P7/64 ,  C11B1/00 ,  A01H5/10 ,  A23D9/00

Abstract: The present invention relates to processes for obtaining a lipid from a cell by lysing the cell, contacting the cell with a base and/or salt, and separating the lipid. The present invention is also directed to a lipid prepared by the processes of the present invention. The present invention is also directed to microbial lipids having a particular anisidine value, peroxide value, and/or phosphorus content.

公开(公告)号：US08309796B2

公开(公告)日：2012-11-13

申请号：US13170011

申请日：2011-06-27

Applicant: Craig A. Weaver ,  Ross Zirkle ,  Daniel H. Doherty ,  James G. Metz

Inventor： Craig A. Weaver ,  Ross Zirkle ,  Daniel H. Doherty ,  James G. Metz

IPC: A01H5/00 ,  C12N1/20

CPC classification number: C12P7/6427 ,  C12N9/93 ,  C12N15/52 ,  C12N15/8247 ,  C12N15/8273 ,  C12P7/62 ,  C12P7/625 ,  C12P7/6472

Abstract: Disclosed are chimeric polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) proteins and chimeric PUFA PKS systems, including chimeric PUFA PKS proteins and systems derived from Schizochytrium and Thraustochytrium. Disclosed are nucleic acids and proteins encoding such chimeric PUFA PKS proteins and systems, genetically modified organisms comprising such chimeric PUFA PKS proteins and systems, and methods of making and using such chimeric PUFA PKS proteins and systems.

Abstract translation: 公开了嵌合多不饱和脂肪酸（PUFA）聚酮化合物合成酶（PKS）蛋白和嵌合PUFA PKS系统，包括嵌合PUFA PKS蛋白和源自裂殖壶菌和破囊壶菌的系统。 公开了编码这种嵌合PUFA PKS蛋白质和系统的核酸和蛋白质，包含这种嵌合PUFA PKS蛋白质和系统的遗传修饰的生物体，以及制备和使用这种嵌合PUFA PKS蛋白质和系统的方法。

公开(公告)号：US07906706B2

公开(公告)日：2011-03-15

申请号：US11689450

申请日：2007-03-21

Applicant: Craig A. Weaver ,  Ross Zirkle ,  James G. Metz

Inventor： Craig A. Weaver ,  Ross Zirkle ,  James G. Metz

IPC: A01H9/00 ,  C12N1/12 ,  C12N15/87 ,  C12P7/64

CPC classification number: C12P7/6472 ,  C07K14/195 ,  C12N9/001 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/8247 ,  C12P7/62 ,  C12P7/6427

Abstract: Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

公开(公告)号：US07897392B2

公开(公告)日：2011-03-01

申请号：US11781875

申请日：2007-07-23

Applicant: Craig A. Weaver ,  Ross Zirkle ,  James G. Metz

Inventor： Craig A. Weaver ,  Ross Zirkle ,  James G. Metz

IPC: C12N15/82 ,  C12N5/12 ,  C12N1/20 ,  C07H21/04

CPC classification number: C12P7/6472 ,  C07K14/195 ,  C12N9/001 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/8247 ,  C12P7/62 ,  C12P7/6427

Abstract: Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

公开(公告)号：US20100239533A1

公开(公告)日：2010-09-23

申请号：US12407687

申请日：2009-03-19

Applicant: Kirk E. APT ,  Joseph W. Pfeifer, III ,  Jon Milton Hansen ,  Paul Warren Behrens ,  Ross Zirkle ,  Tracey Lynn Stahl

Inventor： Kirk E. APT ,  Joseph W. Pfeifer, III ,  Jon Milton Hansen ,  Paul Warren Behrens ,  Ross Zirkle ,  Tracey Lynn Stahl

IPC: A61K35/00 ,  C12N1/10 ,  C07C57/00 ,  C12P7/64 ,  C11B1/00 ,  A61K31/20 ,  A23D9/00 ,  A23C9/20 ,  A23L2/52 ,  A23K1/16 ,  A23K1/18

CPC classification number: A23K20/158 ,  A23D9/00 ,  A23K50/00 ,  A23K50/80 ,  A23L33/115 ,  A23L33/12 ,  A23L33/135 ,  A23V2002/00 ,  A61K8/99 ,  A61K31/20 ,  A61K31/202 ,  A61K36/02 ,  A61K2035/11 ,  A61K2800/74 ,  A61Q19/00 ,  C12P7/6427 ,  C12P7/6472 ,  C12R1/89

Abstract: The present invention is directed to isolated thraustochytrid microorganisms as well as strains and mutants thereof. The invention is further directed to biomasses, microbial oils, compositions, cultures, methods of producing microbial oils, and methods of using the isolated thraustochytrids, biomasses, and microbial oils.

Abstract translation: 本发明涉及分离的破釜状菌属微生物以及其菌株和突变体。 本发明进一步涉及生物量，微生物油，组合物，培养物，生产微生物油的方法，以及使用分离的破釜蛇疮，生物质和微生物油的方法。

公开(公告)号：US20080050791A1

公开(公告)日：2008-02-28

申请号：US11781879

申请日：2007-07-23

Applicant: Craig Weaver ,  Ross Zirkle ,  James Metz

Inventor： Craig Weaver ,  Ross Zirkle ,  James Metz

IPC: C12P7/64 ,  C07H21/00 ,  C12N1/00 ,  C12N1/12 ,  C12N1/21 ,  C12N5/04

CPC classification number: C12P7/6472 ,  C07K14/195 ,  C12N9/001 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/8247 ,  C12P7/62 ,  C12P7/6427

Abstract: Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

公开(公告)号：US20080032338A1

公开(公告)日：2008-02-07

申请号：US11781880

申请日：2007-07-23

Applicant: Craig Weaver ,  Ross Zirkle ,  James Metz

Inventor： Craig Weaver ,  Ross Zirkle ,  James Metz

IPC: C12P21/04 ,  C12N1/20 ,  C12N15/00 ,  C12N15/11 ,  C12N5/04

CPC classification number: C12P7/6472 ,  C07K14/195 ,  C12N9/001 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/8247 ,  C12P7/62 ,  C12P7/6427

Abstract: Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

公开(公告)号：US20080026437A1

公开(公告)日：2008-01-31

申请号：US11689459

申请日：2007-03-21

Applicant: Craig Weaver ,  Ross Zirkle ,  James Metz

Inventor： Craig Weaver ,  Ross Zirkle ,  James Metz

IPC: C12P7/64 ,  C07H21/00 ,  C12N1/00 ,  C12N1/21 ,  C12N5/04

CPC classification number: C12P7/6472 ,  C07K14/195 ,  C12N9/001 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/8247 ,  C12P7/62 ,  C12P7/6427

Abstract: Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

公开(公告)号：US20050100995A1

公开(公告)日：2005-05-12

申请号：US10965017

申请日：2004-10-13

Applicant: Craig Weaver ,  Ross Zirkle ,  James Metz

Inventor： Craig Weaver ,  Ross Zirkle ,  James Metz

IPC: C07H21/04 ,  C12N1/21 ,  C12N9/02 ,  C12N9/10 ,  C12N15/74 ,  C12P7/64

CPC classification number: C12P7/6472 ,  C07K14/195 ,  C12N9/001 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/8247 ,  C12P7/62 ,  C12P7/6427

Abstract: Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

Abstract translation: 公开了来自细菌微生物Shewanella japonica和Shewanella olleyana的完全多不饱和脂肪酸（PUFA）聚酮化合物（PKS）系统及其生物活性片段和同系物。 更具体地，本发明涉及编码这种PUFA PKS系统的核酸，其包含这种PUFA PKS系统的蛋白质和结构域到包含这种PUFA PKS系统的转基因生物（植物和微生物），以及制备和使用 本文公开的PUFA PKS系统。 本发明还涉及基因修饰的植物和微生物以及通过操作PUFA聚酮化合物合酶（PKS）系统来有效地产生富含多种不饱和脂肪酸（PUFA）以及其它生物活性分子的脂质的方法。

公开(公告)号：US06858404B2

公开(公告)日：2005-02-22

申请号：US10014717

申请日：2001-11-13

Applicant: Thomas Schupp ,  James Madison Ligon ,  Istvan Molnar ,  Ross Zirkle ,  Devon Dawn Cyr ,  Jörn Görlach

Inventor： Thomas Schupp ,  James Madison Ligon ,  Istvan Molnar ,  Ross Zirkle ,  Devon Dawn Cyr ,  Jörn Görlach

IPC: C07K14/195 ,  C12N1/21 ,  C12P17/18 ,  C12P1/00

CPC classification number: C07K14/195 ,  C12P17/181

Abstract: Nucleic acid molecules are isolated from Sorangium cellulosum that encode polypeptides necessary for the biosynthesis of epothilone. Disclosed are methods for the production of epothilone in recombinant hosts transformed with the genes of the invention. In this manner, epothilone can be produced in quantities large enough to enable their purification and use in pharmaceutical formulations such as those for the treatment of cancer.

Abstract translation: 核酸分子从编码为埃坡霉素生物合成所必需的多肽的纤维索兰分离。 披露了用本发明的基因转化的重组宿主中埃坡霉素生产的方法。 以这种方式，埃坡霉素可以以足够大的量生产，以使其能够在诸如用于治疗癌症的药物制剂中进行纯化和使用。