公开(公告)号：US20100240051A1

公开(公告)日：2010-09-23

申请号：US12716031

申请日：2010-03-02

Applicant: Jeff Tza-Huei Wang ,  Yi Zhang

Inventor： Jeff Tza-Huei Wang ,  Yi Zhang

IPC: C12Q1/68 ,  C12M1/34 ,  B05D3/00

CPC classification number: B01L3/502715 ,  B01J2219/00313 ,  B01J2219/00722 ,  B01L3/502723 ,  B01L3/502769 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/0673 ,  B01L2200/0684 ,  B01L2200/141 ,  B01L2300/0803 ,  B01L2300/0864 ,  B01L2400/0406 ,  C12Q1/686 ,  C12Q2547/107 ,  C12Q2523/125

Abstract: The present invention relates to methods, kits and devices with multiple open reaction chambers having multiple pre-deposited primer compositions, and a basic sample loading mechanism that utilizes an immiscible companion fluid for preparing and performing multiple Polymerase Chain Reactions

Abstract translation: 本发明涉及具有多个预先沉积的底漆组合物的多个开放反应室的方法，试剂盒和装置，以及利用不混溶伴侣流体制备和进行多重聚合酶链反应的基本样品加载机理

公开(公告)号：US20130303394A1

公开(公告)日：2013-11-14

申请号：US13856320

申请日：2013-04-03

Applicant: Jeff Tza-Huei Wang ,  Yi Zhang

Inventor： Jeff Tza-Huei Wang ,  Yi Zhang

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  B01L3/5085 ,  B01L3/5088 ,  B01L2200/12 ,  B81B2201/058 ,  B81C1/00206 ,  C03C17/328

Abstract: A method of using a fluidic device includes providing a fluidic chip having a plurality of surface energy traps; depositing at least one fluid droplet on the fluidic chip, the fluid droplet including magnetic particles suspended therein; and moving at least a portion of the at least one fluid droplet across a surface of the fluidic chip by altering a magnetic interaction applied to the magnetic particles such that the at least one fluid droplet interacts with at least one of the plurality of surface energy traps.

Abstract translation: 使用流体装置的方法包括提供具有多个表面能量阱的流体碎片; 在流体芯片上沉积至少一个流体液滴，所述流体液滴包括悬浮在其中的磁性颗粒; 以及通过改变施加到所述磁性颗粒的磁相互作用来移动所述至少一个流体液滴的至少一部分穿过所述流体芯片的表面，使得所述至少一个流体液滴与所述多个表面能量阱中的至少一个相互作用 。

公开(公告)号：US20110287976A1

公开(公告)日：2011-11-24

申请号：US13147742

申请日：2010-03-02

Applicant: Jeff Tza-Huei Wang ,  Kelvin J. Liu ,  Christopher M. Puleo ,  Tushar D. Rane

Inventor： Jeff Tza-Huei Wang ,  Kelvin J. Liu ,  Christopher M. Puleo ,  Tushar D. Rane

IPC: C40B30/10 ,  C40B60/12

CPC classification number: C12Q1/6825 ,  B01L3/502753 ,  B01L3/502769 ,  B01L7/52 ,  B01L2200/0678 ,  B01L2200/10 ,  B01L2300/088 ,  B01L2400/0481 ,  B01L2400/0655 ,  G01N1/38 ,  G01N15/1484 ,  G01N21/6458 ,  G01N2001/4027 ,  G01N2015/0038 ,  G01N2015/1493 ,  G01N2021/6419 ,  G01N2021/6421 ,  Y10T436/143333

Abstract: A microfluidic device for a confocal fluorescence detection system has an input channel defined by a body of the microfluidic device, a sample concentration section defined by the body of the microfluidic device and in fluid connection with the input channel, a mixing section defined by the body of the microfluidic device and in fluid connection with the concentration section, and a detection region that is at least partially transparent to illumination light of the confocal fluorescence detection system and at least partially transparent to fluorescent light when emitted from a sample under observation as the sample flows through the detection region.

Abstract translation: 用于共焦荧光检测系统的微流体装置具有由微流体装置的主体限定的输入通道，由微流体装置的主体限定并与输入通道流体连接的样品浓度部分，由身体限定的混合部分 的微流体装置，并且与浓缩部分流体连接;以及检测区域，其对于共聚焦荧光检测系统的照明光至少部分透明，并且当从作为样品的观察样品发射时对荧光至少部分透明 流过检测区域。

公开(公告)号：US08637301B2

公开(公告)日：2014-01-28

申请号：US13147742

申请日：2010-03-02

Applicant: Jeff Tza-Huei Wang ,  Kelvin J. Liu ,  Christopher M. Puleo ,  Tushar D. Rane

Inventor： Jeff Tza-Huei Wang ,  Kelvin J. Liu ,  Christopher M. Puleo ,  Tushar D. Rane

IPC: C12M1/00 ,  C12M1/36 ,  C12M1/34 ,  G01N15/06 ,  G01N21/64 ,  G02B21/06

CPC classification number: C12Q1/6825 ,  B01L3/502753 ,  B01L3/502769 ,  B01L7/52 ,  B01L2200/0678 ,  B01L2200/10 ,  B01L2300/088 ,  B01L2400/0481 ,  B01L2400/0655 ,  G01N1/38 ,  G01N15/1484 ,  G01N21/6458 ,  G01N2001/4027 ,  G01N2015/0038 ,  G01N2015/1493 ,  G01N2021/6419 ,  G01N2021/6421 ,  Y10T436/143333

Abstract: A microfluidic device for a confocal fluorescence detection system has an input channel defined by a body of the microfluidic device, a sample concentration section defined by the body of the microfluidic device and in fluid connection with the input channel, a mixing section defined by the body of the microfluidic device and in fluid connection with the concentration section, and a detection region that is at least partially transparent to illumination light of the confocal fluorescence detection system and at least partially transparent to fluorescent light when emitted from a sample under observation as the sample flows through the detection region.

Abstract translation: 用于共焦荧光检测系统的微流体装置具有由微流体装置的主体限定的输入通道，由微流体装置的主体限定并与输入通道流体连接的样品浓度部分，由身体限定的混合部分 的微流体装置，并且与浓缩部分流体连接;以及检测区域，其对于共焦荧光检测系统的照明光至少部分透明，并且当从作为样品的观察样品发射时对荧光至少部分透明 流过检测区域。

公开(公告)号：US20120135874A1

公开(公告)日：2012-05-31

申请号：US13260212

申请日：2010-05-06

Applicant: Jeff Tza-Huei Wang ,  Kelvin J. Liu ,  Christopher M. Puleo

Inventor： Jeff Tza-Huei Wang ,  Kelvin J. Liu ,  Christopher M. Puleo

IPC: G01N21/64 ,  B01L3/00 ,  C40B30/04 ,  B82Y15/00

CPC classification number: C12Q1/6844 ,  C12Q2563/143 ,  C12Q2549/119 ,  C12Q2537/149

Abstract: The present invention relates, e.g., to a method for detecting a nucleic acid molecule of interest in a sample comprising cell-free nucleic acids, comprising fluorescently labeling the nucleic acid molecule of interest, by specifically binding a fluorescently labeled nanosensor or probe to the nucleic acid of interest, or by enzymatically incorporating a fluorescent probe or dye into the nucleic acid of interest, illuminating the fluorescently labeled nucleic acid molecule, causing it to emit fluorescent light, and measuring the level of fluorescence by single molecule spectroscopy, wherein the detection of a fluorescent signal is indicative of the presence of the nucleic acid of interest in the sample.

Abstract translation: 本发明涉及例如用于检测包含无细胞核酸的样品中感兴趣的核酸分子的方法，其包括通过将荧光标记的纳米传感器或探针特异性结合到核酸上来荧光标记感兴趣的核酸分子 酸，或通过将荧光探针或染料酶促并入感兴趣的核酸，照射荧光标记的核酸分子，使其发射荧光，并通过单分子光谱法测量荧光水平，其中检测 荧光信号指示样品中感兴趣的核酸的存在。