公开(公告)号：US4739171A

公开(公告)日：1988-04-19

申请号：US789994

申请日：1985-10-22

Applicant: Jonathan Briggs

Inventor： Jonathan Briggs

IPC: G01N21/64 ,  G01N33/542 ,  G01T1/10

CPC classification number: G01N33/542 ,  G01N21/6428 ,  G01N2201/08

Abstract: A method and apparatus for determining the presence of particles in a fluorescent dispersion which exceed either a predetermined fluorescence intensity or size are disclosed. The method and apparatus involve the irradiation of the dispersion with excitation light, the examination of the fluorescent light entering an optical fiber from a portion of the excited region small enough to permit only a low probability that more than one particle of interest is present in the volume during a predetermined period of time, and the measurement of the emitted fluorescence entering the fiber, substantially free of excitation light, to the extent that the fluorescence differs in intensity from that of the dispersion in the absence of the particle.

Abstract translation: 公开了一种用于确定荧光分散体中超过预定荧光强度或尺寸的颗粒的存在的方法和装置。 该方法和装置包括用激发光照射分散体，从激发区域的一部分进入光纤的荧光的检查小到足以允许仅有一个以上感兴趣的颗粒存在的可能性低 在预定时间段内的体积，以及基本上不含激发光的进入纤维的发射荧光的测量，使得在不存在颗粒的情况下荧光的强度与分散体的强度不同。

公开(公告)号：US4737464A

公开(公告)日：1988-04-12

申请号：US780485

申请日：1985-09-26

Applicant: Harden M. McConnell ,  Jonathan Briggs ,  John W. Parce

Inventor： Harden M. McConnell ,  Jonathan Briggs ,  John W. Parce

IPC: G01N21/25 ,  G01N21/27 ,  G01N21/00

CPC classification number: G01N21/253 ,  G01N21/272 ,  G01N21/6428 ,  G01N2201/0461 ,  Y10S436/805 ,  Y10S436/807 ,  Y10T436/11

Abstract: An apparatus for determining a characteristic of a signal modulating component related to an analyte of interest at an assay site by measuring light modulated by the component. A light source uniformly illuminates the assay site and a reference site. The light is directed from the sites onto a group of memory cells on the surface of a random access memory. The memory cells will decay from a logical 1 to a logical 0 due to the light over a period of time which is a function of the intensity of the light and the reference voltage of the memory cells marking the difference between a 1 and a 0. The digital state of each of the memory cells in the group is measured a predetermined exposure time. The measured states of a group of memory cells corresponding to the assay site are compared to the memory cell states for a reference group. The measured states are then analyzed to determine a characteristic of the analyte.

Abstract translation: 一种用于通过测量由该组分调制的光来确定在测定部位与感兴趣分析物相关的信号调节成分的特征的装置。 光源均匀地照射了测定部位和参考部位。 光从站点引导到随机存取存储器表面上的一组存储器单元上。 存储器单元将由于在一段时间内的光而从逻辑1衰减到逻辑0，该时间段是光的强度和记录单元的参考电压的函数，标记1和0之间的差。 在预定的曝光时间内测量组中的每个存储单元的数字状态。 将对应于测定位点的一组存储器单元的测量状态与参考组的存储器单元状态进行比较。 然后分析测量的状态以确定分析物的特征。

公开(公告)号：US5560811A

公开(公告)日：1996-10-01

申请号：US408683

申请日：1995-03-21

Applicant: Jonathan Briggs ,  Randy M. McCormick ,  David W. Hoyt

Inventor： Jonathan Briggs ,  Randy M. McCormick ,  David W. Hoyt

IPC: B82B1/00 ,  G01N27/447 ,  G01N35/00 ,  B01D57/02 ,  B01D59/42 ,  C07K1/26 ,  C25B7/00

CPC classification number: G01N27/44782 ,  B01L9/065 ,  G01N27/44743 ,  G01N2035/00237

Abstract: This invention involves method and apparatus for multiplexing electrophoresis analysis. An array of samples in multi well plates are simultaneously transferred to an array of electrophoresis column where electrophoresis is simultaneously carried out followed by analysis of the columns. The methods and apparatus of this invention are, for example, useful for DNA analysis, including sequencing, and for measuring reactions between specifically binding proteins and their binding partners.

Abstract translation: 本发明涉及多重电泳分析的方法和装置。 同时将多孔板中的样品阵列转移到电泳柱阵列，同时进行电泳，然后分析色谱柱。 本发明的方法和装置例如可用于DNA分析，包括测序，以及用于测量特异性结合蛋白质及其结合配偶体之间的反应。

公开(公告)号：US4956275A

公开(公告)日：1990-09-11

申请号：US38294

申请日：1987-04-14

Applicant: Robert F. Zuk ,  Richard D. Armenta ,  Jonathan Briggs

Inventor： Robert F. Zuk ,  Richard D. Armenta ,  Jonathan Briggs

IPC: G01N33/543 ,  G01N33/558 ,  G01N37/00

CPC classification number: G01N33/54386 ,  G01N33/558 ,  Y10S435/805 ,  Y10S435/97 ,  Y10S436/807 ,  Y10S436/81

Abstract: Novel methods and devices are provided for detecting analytes, where the sample is placed upstream from a reagent which is a component of a signal-producing system. The detection zone is provided upstream from the sample, where the amount of reagent which becomes bound at two sites is determined and the two values used for an accurate determination of an analyte.

Abstract translation: 提供了用于检测分析物的新方法和装置，其中将样品置于作为信号产生系统的组分的试剂的上游。 检测区设置在样品的上游，其中确定在两个位点处结合的试剂的量，并且将两个值用于准确测定分析物。

公开(公告)号：US4564598A

公开(公告)日：1986-01-14

申请号：US397285

申请日：1982-07-12

Applicant: Jonathan Briggs

Inventor： Jonathan Briggs

IPC: G01N15/14 ,  G01N15/02 ,  G01N15/06 ,  G01N21/64 ,  G01N33/48 ,  G01N33/49 ,  G01N33/542 ,  G01N33/543 ,  G01N33/50

CPC classification number: G01N21/6428 ,  G01N33/542 ,  G01N2015/0222 ,  G01N2021/6484 ,  G01N2201/08 ,  Y10S436/805

Abstract: A method and apparatus for determining the presence of particles in a fluorescent dispersion which exceed either a predetermined fluorescence intensity or size are disclosed. The method and apparatus involve the irradiation of the dispersion with excitation light, the examination of the fluorescent light entering an optical fiber from a portion of the excited region small enough to permit only a low probability that more than one particle of interest is present in the volume during a predetermined period of time, and the measurement of the emitted fluorescence entering the fiber, substantially free of excitation light, to the extent that the fluorescence differs in intensity from that of the dispersion in the absence of the particle.

Abstract translation: 公开了一种用于确定荧光分散体中超过预定荧光强度或尺寸的颗粒的存在的方法和装置。 该方法和装置包括用激发光照射分散体，从激发区域的一部分进入光纤的荧光的检查小到足以允许仅有一个以上感兴趣的颗粒存在的可能性低 在预定时间段内的体积，以及基本上不含激发光的进入纤维的发射荧光的测量，使得在不存在颗粒的情况下荧光的强度与分散体的强度不同。

公开(公告)号：US6043036A

公开(公告)日：2000-03-28

申请号：US977931

申请日：1997-11-24

Applicant: Randy M. McCormick ,  Jonathan Briggs

Inventor： Randy M. McCormick ,  Jonathan Briggs

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6869 ,  G06F19/22

Abstract: The present invention describes a method of sequencing nucleic acids in which mixtures of oligonucleotide fragments are derived from sequencing reactions using combinations of the 2',3'-dideoxynucleoside 5'-triphosphate or 3' deoxynucleoside 5'-triphosphate terminators and appropriate concentrations of four dNTPs (2'-deoxynucleoside 5' triphosphates, e.g., dATP, dCTP, dGTP, dTTP, dITP, 7-deaza-GTP). These fragments are generated by enzymatic extension of a primer hybridized to the single-stranded template DNA to be sequenced. In contrast to common slab gel sequencing methods, the method of the instant invention does not require precise alignment of the four separation sets of the terminated fragments to permit deduction of the DNA sequence. In addition, the method possesses inherent redundancy in the separations, which facilitates sequence assignment by resolving sequence uncertainties or anomalies.

Abstract translation: 本发明描述了使用2'，3'-双脱氧核苷5'-三磷酸终止子和3'脱氧核苷5'-三磷酸终止子的组合以及合适浓度的4个核苷酸的序列来测序核酸的方法，其中寡核苷酸片段的混合物来源于测序反应 dNTPs（2'-脱氧核苷5'三磷酸，例如dATP，dCTP，dGTP，dTTP，dITP，7-脱氮-GTP）。 这些片段通过与要测序的单链模板DNA杂交的引物的酶促延伸产生。 与普通平板凝胶测序方法相反，本发明的方法不需要对终止的片段的四个分离组进行精确的比对以允许推断DNA序列。 此外，该方法在分离中具有固有的冗余性，这有助于通过解析序列不确定性或异常来进行序列分配。

公开(公告)号：US5529752A

公开(公告)日：1996-06-25

申请号：US361980

申请日：1994-12-22

Applicant: George Pontis ,  Michael Lacy ,  Vladimir Drbal ,  Calvin Chow ,  Thomas Smith ,  Rick Smethers ,  Debra A. Ross ,  Gregory Kirk ,  Jonathan Briggs ,  Lev Leytes ,  Suresh N. Mehta

Inventor： George Pontis ,  Michael Lacy ,  Vladimir Drbal ,  Calvin Chow ,  Thomas Smith ,  Rick Smethers ,  Debra A. Ross ,  Gregory Kirk ,  Jonathan Briggs ,  Lev Leytes ,  Suresh N. Mehta

IPC: G01N33/53 ,  G01N33/543 ,  G01N35/00 ,  G01N35/10

CPC classification number: G01N35/00009 ,  G01N33/5302 ,  G01N33/54366 ,  G01N35/00029 ,  G01N2035/00138 ,  G01N2035/00485 ,  Y10S435/97 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/25 ,  Y10T436/25375

Abstract: This invention encompasses a work station for conducting assays which comprises filtering assembly, a slide reading assembly, and a slide with a porous filter membrane wherein the slide has a means for aligning the slide within the filter assembly to filter reagents on predetermined portions of the porous filter membrane and also to position the slide in the slide reading assembly so that detection will occur in those same predetermined locations.

Abstract translation: 本发明包括用于进行测定的工作站，其包括过滤组件，滑动读取组件和具有多孔过滤膜的滑块，其中滑块具有用于使过滤器组件内的滑块对准在多孔过滤器的预定部分上过滤试剂的装置 过滤膜并且还将滑块定位在滑动读取组件中，使得检测将发生在那些相同的预定位置。

公开(公告)号：US3940117A

公开(公告)日：1976-02-24

申请号：US480355

申请日：1974-06-18

Applicant: Charles Jonathan Briggs

Inventor： Charles Jonathan Briggs

IPC: B01F13/10 ,  B01F9/12

CPC classification number: B01F13/1058

Abstract: An agitating apparatus comprising a support, a container supported by the support and rotatable relative to the support about a substantially vertical axis, an agitator within the container and co-operating drive means between the support and the upper part of the container for driving said agitator when the container is rotated about said axis.

Abstract translation: 一种搅拌装置，包括支撑件，由支撑件支撑并且围绕基本垂直的轴线可相对于支撑件旋转的容器，容器内的搅拌器以及在支撑件和容器的上部之间的协同驱动装置，用于驱动所述搅拌器 当容器围绕所述轴线旋转时。

公开(公告)号：US20110233426A1

公开(公告)日：2011-09-29

申请号：US12949219

申请日：2010-11-18

Applicant: Jonathan Briggs CARROLL ,  Paul Martens

Inventor： Jonathan Briggs CARROLL ,  Paul Martens

IPC: G01J1/58 ,  F42B27/00

CPC classification number: F42B12/40

Abstract: A method and apparatus that are useful for tagging personnel for identification and tracking is disclosed. The apparatus may include a taggant, the taggant being a chemical substance that adheres to at least one of human skin, clothing and equipment of personnel and is undetectable by the human eye, and a taggant deployment mechanism that includes the taggant and is configured to release the taggant upon activation of a triggering mechanism, wherein the taggant deployment mechanism is configured such that when the triggering mechanism is activated, the taggant is released from the taggant deployment mechanism and adheres to at least one of human skin, clothing and equipment of personnel within a radius of a point that the taggant is released, the taggant being detectable only by a taggant detection unit.

Abstract translation: 公开了一种用于识别和跟踪人员标签的方法和装置。 该装置可以包括标签牌，该标签牌是粘附在人的皮肤，衣物和人员中的至少一种并且被人眼检测到的化学物质，以及包括标签牌并被配置为释放的标签牌展开机构 所述标签牌在激活触发机构时，其中所述标签牌展开机构构造成使得当所述触发机构被激活时，所述标签牌从所述标签牌展开机构中被释放并且粘附在所述标签牌展开机构中的人体皮肤，衣物和设备中的至少一个 标签牌被释放的点的半径，标签牌只能由标签牌检测单元检测。

公开(公告)号：US07662973B2

公开(公告)日：2010-02-16

申请号：US10182996

申请日：2001-02-01

Applicant: Nicholas Thomas ,  Nigel Paul Michael ,  Valerie Millar ,  Beth Davies ,  Mark Samuel Jonathan Briggs

Inventor： Nicholas Thomas ,  Nigel Paul Michael ,  Valerie Millar ,  Beth Davies ,  Mark Samuel Jonathan Briggs

IPC: C09B7/02

CPC classification number: C09B23/08 ,  C09B67/0033 ,  G01N33/84

Abstract: Disclosed is a method for increasing the fluorescence of a Cyanine dye molecule comprising at least one NO2 group characterized by the reduction of the at least one NO2 group to NHOH or NH2 by the action of a nitroreductase. The cyanine dye molecule comprising at least one NO2 group can be used as a substrate for detecting nitroreductase enzyme activity in a composition and allows for the use of a nitroreductase enzyme in an enzyme-reporter system for the detection of analytes, binding reactions or gene expression.

Abstract translation: 公开了增加包含至少一个NO 2基团的花青染料分子的荧光的方法，其特征在于通过硝基还原酶的作用将至少一种NO 2基团还原成NHOH或NH 2。 包含至少一个NO 2基团的花青染料分子可用作组合物中硝基还原酶活性检测的底物，并且允许在酶报告系统中使用硝基还原酶用于检测分析物，结合反应或基因表达 。