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公开(公告)号：US08546482B2
公开(公告)日：2013-10-01
申请号：US12928409
申请日：2010-12-10
CPC分类号： C08L33/04 , C08F6/006 , C08L2201/52 , C09D131/04 , C09D133/04 , C09D133/08 , C09D135/06 , C08L33/00
摘要： This invention provides stable aqueous compositions including stable coating compositions, and methods of their production. The stable aqueous compositions and stable aqueous coating compositions comprise one or more emulsion-polymerized addition polymer, one or more carboxylesterase enzyme, and one or more carboxylesterase deactivating agent, such that the ester hydrolysis activity in the composition is less than 0.010 micromole/minute, and wherein the aqueous composition has a headspace volatile organic compound (VOC) content, as measured by headspace gas chromatography-mass spectrometry (GC-MS) at 33° C., of less than 10 ppm of organic carboxylester with a normal boiling point of less than 150° C. and more than 50 ppm of mono-alcohol with a formula molecular weight of less than 76. The carboxylesterase enzyme is employed to minimize the free carboxylester content in the composition, thereby providing a low odor composition, and the carboxylesterase is deactivated in situ in order to minimize the residual carboxylesterase enzyme activity.
摘要翻译： 本发明提供稳定的含水组合物，包括稳定的涂料组合物及其生产方法。 稳定的水性组合物和稳定的水性涂料组合物包含一种或多种乳液聚合的加成聚合物，一种或多种羧酸酯酶和一种或多种羧酸酯酶失活剂，使得组合物中的酯水解活性小于0.010微摩尔/分钟， 并且其中所述含水组合物具有顶空挥发性有机化合物（VOC）含量，如通过顶空气相色谱 - 质谱（GC-MS）在33℃下测得的，小于10ppm的有机羧酸酯的正常沸点为 低于150℃和超过50ppm的具有式分子量小于76的单醇。羧酸酯酶用于使组合物中游离羧酸酯含量最小化，从而提供低气味组合物，并且羧酸酯酶 在原位失活，以使残留的羧酸酯酶活性最小化。
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公开(公告)号：US06559297B2
公开(公告)日：2003-05-06
申请号：US09895752
申请日：2001-06-29
IPC分类号： C12N900
CPC分类号： C12N9/1085 , C12N9/00 , C12N9/88 , C12N15/8243
摘要： Novel synthases and the corresponding nucleic acids encoding such synthases are disclosed herein. Such synthases possess an active site pocket that includes key amino acid residues that are modified to generate desired terpenoid reaction intermediates and products. Synthase modifications are designed based on, e.g., the three-dimensional coordinates of tobacco 5-epi-aristolochene synthase, with or without a substrate bound in the active site.
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公开(公告)号：US06495354B2
公开(公告)日：2002-12-17
申请号：US09887586
申请日：2001-06-22
IPC分类号： C12N900
CPC分类号： C12N9/1085 , C12N9/00 , C12N9/88 , C12N15/8243
摘要： Novel synthases and the corresponding nucleic acids encoding such synthases are disclosed herein. Such synthases possess an active site pocket that includes key amino acid residues that are modified to generate desired terpenoid reaction intermediates and products. Synthase modifications are designed based on, e.g., the three-dimensional coordinates of tobacco 5-epi-aristolochene synthase with or without a substrate bound in the active site.
摘要翻译： 本文公开了新型合酶和编码此类合酶的相应核酸。 这种合酶具有活性位点口袋，其包括被修饰以产生所需萜类化合物中间体和产物的关键氨基酸残基。 合成酶修饰是基于例如具有或不具有结合在活性位点中的底物的烟草5-epi-aristolochene合酶的三维坐标来设计的。
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公开(公告)号：US06468772B1
公开(公告)日：2002-10-22
申请号：US09398395
申请日：1999-09-17
IPC分类号： C12N900
CPC分类号： C12N9/1085 , C12N9/00 , C12N9/88 , C12N15/8243
摘要： Novel polypeptides and the corresponding nucleic acids encoding such polypeptides are disclosed herein. The invention provides methods of making modified polypeptides by altering one or more amino acid residues involved in the active site of a preselected polypeptide.
摘要翻译： 本文公开了新型多肽和编码此类多肽的相应核酸。 本发明提供了通过改变参与预选多肽的活性位点的一个或多个氨基酸残基来制备修饰的多肽的方法。
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公开(公告)号：US20110152406A1
公开(公告)日：2011-06-23
申请号：US12928409
申请日：2010-12-10
IPC分类号： C09D133/12 , C09D189/00 , C08J3/00
CPC分类号： C08L33/04 , C08F6/006 , C08L2201/52 , C09D131/04 , C09D133/04 , C09D133/08 , C09D135/06 , C08L33/00
摘要： This invention provides stable aqueous compositions including stable coating compositions, and methods of their production. The stable aqueous compositions and stable aqueous coating compositions comprise one or more emulsion-polymerized addition polymer, one or more carboxylesterase enzyme, and one or more carboxylesterase deactivating agent, such that the ester hydrolysis activity in the composition is less than 0.010 micromole/minute, and wherein the aqueous composition has a headspace volatile organic compound (VOC) content, as measured by headspace gas chromatography-mass spectrometry (GC-MS) at 33° C., of less than 10 ppm of organic carboxylester with a normal boiling point of less than 150° C. and more than 50 ppm of mono-alcohol with a formula molecular weight of less than 76. The carboxylesterase enzyme is employed to minimize the free carboxylester content in the composition, thereby providing a low odor composition, and the carboxylesterase is deactivated in situ in order to minimize the residual carboxylesterase enzyme activity.
摘要翻译： 本发明提供稳定的含水组合物，包括稳定的涂料组合物及其生产方法。 稳定的水性组合物和稳定的水性涂料组合物包含一种或多种乳液聚合的加成聚合物，一种或多种羧酸酯酶和一种或多种羧酸酯酶失活剂，使得组合物中的酯水解活性小于0.010微摩尔/分钟， 并且其中所述含水组合物具有顶空挥发性有机化合物（VOC）含量，如通过顶空气相色谱 - 质谱（GC-MS）在33℃下测得的，小于10ppm的有机羧酸酯的正常沸点为 低于150℃和超过50ppm的具有式分子量小于76的单醇。羧酸酯酶用于使组合物中游离羧酸酯含量最小化，从而提供低气味组合物，并且羧酸酯酶 在原位失活，以使残留的羧酸酯酶活性最小化。
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公开(公告)号：US06569656B2
公开(公告)日：2003-05-27
申请号：US09903012
申请日：2001-07-11
IPC分类号： C12N900
CPC分类号： C12N9/1085 , C12N9/00 , C12N9/88 , C12N15/8243
摘要： Novel synthases and the corresponding nucleic acids encoding such synthases are disclosed herein. Such synthases possess an active site pocket that includes key amino acid residues that are modified to generate desired terpenoid reaction intermediates and products. Synthase modifications are designed based on, e.g., the three-dimensional coordinates of tobacco 5-epi-aristolochene synthase, with or without a substrate bound in the active site.
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公开(公告)号：US08524809B2
公开(公告)日：2013-09-03
申请号：US12928408
申请日：2010-12-10
CPC分类号： C08F6/006 , C09D131/04 , C08L33/06 , C08L31/02
摘要： This invention provides stable aqueous compositions including stable coating compositions, and methods of their production. The stable aqueous compositions and stable aqueous coating compositions comprise i) an aqueous dispersion of one or more emulsion-polymerized addition polymer comprising polymerized units of one or more carboxylester monomer, wherein at least one carboxylester monomer is a vinyl ester monomer; ii) one or more carboxylesterase enzyme; iii) one or more mono-alcohol with a formula molecular weight of less than 76; iv) optionally, acetaldehyde; and v) optionally, one or more organic carboxylester with a normal boiling point of less than 150° C.; and wherein the aqueous composition has a headspace volatile organic compound (VOC) content, as measured by headspace gas chromatography-mass spectrometry (GC-MS) at 33° C., characterized by a content of less than 10 ppm of acetaldehyde, and less than 10 ppm of the one or more organic carboxylester, and more than 50 ppm of the one or more mono-alcohol. Preferably, the ester hydrolysis activity in the composition is less than 0.010 micromole/minute.
摘要翻译： 本发明提供稳定的含水组合物，包括稳定的涂料组合物及其生产方法。 稳定的含水组合物和稳定的水性涂料组合物包含i）一种或多种乳液聚合的加成聚合物的水分散体，其包含一个或多个羧酸酯单体的聚合单元，其中至少一个羧酸酯单体是乙烯基酯单体; ii）一种或多种羧酸酯酶; iii）一种或多种具有式分子量小于76的单醇; iv）任选的乙醛; 和v）任选的一种或多种正常沸点低于150℃的有机羧酸酯; 并且其中所述含水组合物具有顶空挥发性有机化合物（VOC）含量，其通过顶空气相色谱 - 质谱（GC-MS）在33℃下测量，其特征在于小于10ppm的乙醛含量和较少 超过10ppm的一种或多种有机羧酸酯，以及大于50ppm的一种或多种单醇。 优选地，组合物中的酯水解活性小于0.010微摩尔/分钟。
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公开(公告)号：US06645762B2
公开(公告)日：2003-11-11
申请号：US09900797
申请日：2001-07-06
IPC分类号： C12N500
CPC分类号： C12N9/1085 , C12N9/00 , C12N9/88 , C12N15/8243
摘要： Novel synthases and the corresponding nucleic acids encoding such synthases are disclosed herein. Such synthases possess an active site pocket that includes key amino acid residues that are modified to generate desired terpenoid reaction intermediates and products. Synthase modifications are designed based on, e.g., the three-dimensional coordinates of tobacco 5-epi-aristolochene synthase, with or without a substrate bound in the active site.
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公开(公告)号：US20110166257A1
公开(公告)日：2011-07-07
申请号：US12928408
申请日：2010-12-10
IPC分类号： C09D131/04 , C09D189/00 , C08J3/00 , C09D133/06
CPC分类号： C08F6/006 , C09D131/04 , C08L33/06 , C08L31/02
摘要： This invention provides stable aqueous compositions including stable coating compositions, and methods of their production. The stable aqueous compositions and stable aqueous coating compositions comprise i) an aqueous dispersion of one or more emulsion-polymerized addition polymer comprising polymerized units of one or more carboxylester monomer, wherein at least one carboxylester monomer is a vinyl ester monomer; ii) one or more carboxylesterase enzyme; iii) one or more mono-alcohol with a formula molecular weight of less than 76; iv) optionally, acetaldehyde; and v) optionally, one or more organic carboxylester with a normal boiling point of less than 150° C.; and wherein the aqueous composition has a headspace volatile organic compound(VOC) content, as measured by headspace gas chromatography-mass spectrometry (GC-MS) at 33° C., characterized by a content of less than 10 ppm of acetaldehyde, and less than 10 ppm of the one or more organic carboxylester, and more than 50 ppm of the one or more mono-alcohol. Preferably, the ester hydrolysis activity in the composition is less than 0.010 micromole/minute.
摘要翻译： 本发明提供稳定的含水组合物，包括稳定的涂料组合物及其生产方法。 稳定的含水组合物和稳定的水性涂料组合物包含i）一种或多种乳液聚合的加成聚合物的水分散体，其包含一个或多个羧酸酯单体的聚合单元，其中至少一个羧酸酯单体是乙烯基酯单体; ii）一种或多种羧酸酯酶; iii）一种或多种具有式分子量小于76的单醇; iv）任选的乙醛; 和v）任选的一种或多种正常沸点低于150℃的有机羧酸酯; 并且其中所述含水组合物具有顶空挥发性有机化合物（VOC）含量，其通过顶空气相色谱 - 质谱（GC-MS）在33℃下测量，其特征在于小于10ppm的乙醛含量和较少 超过10ppm的一种或多种有机羧酸酯，以及大于50ppm的一种或多种单醇。 优选地，组合物中的酯水解活性小于0.010微摩尔/分钟。
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公开(公告)号：US06890752B2
公开(公告)日：2005-05-10
申请号：US09893820
申请日：2001-06-28
IPC分类号： A01H5/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/10 , C12N9/12 , C12N9/88 , C12N15/09 , C12N15/82 , C12Q1/25 , C12R1/91 , G01N33/68 , C12N5/00
CPC分类号： C12N9/1085 , C12N9/00 , C12N9/88 , C12N15/8243
摘要： Novel synthases and the corresponding nucleic acids encoding such synthases are disclosed herein. Such synthases possess an active site pocket that includes key amino acid residues that are modified to generate desired terpenoid reaction intermediates and products. Synthase modifications are designed based on, e.g., the three-dimensional coordinates of tobacco 5-epi-aristolochene synthase. with or without a substrate bound in the active site.
摘要翻译： 本文公开了新型合酶和编码此类合酶的相应核酸。 这种合酶具有活性位点口袋，其包括被修饰以产生所需萜类化合物中间体和产物的关键氨基酸残基。 基于例如烟草5-epi-aristolochene合酶的三维坐标设计合成酶修饰。 有或没有底物结合在活性部位。
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