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公开(公告)号：US07608435B2
公开(公告)日：2009-10-27
申请号：US10582166
申请日：2004-11-18
申请人： Young-Hoon Park , Jea-Young Chang , Jin-Nam Lee , Ki-Hoon Oh , Jeong-Hwan Kim , Yoon-Suk Oh , Jae-Ick Sim
发明人： Young-Hoon Park , Jea-Young Chang , Jin-Nam Lee , Ki-Hoon Oh , Jeong-Hwan Kim , Yoon-Suk Oh , Jae-Ick Sim
CPC分类号： C12P19/32 , C12R1/15 , Y10S435/822 , Y10S435/843
摘要： The invention relates to a microorganism, obtained by treating Corynebacterium ammoniagenes KCCM 10488 producing 5′-Xanthylic acid as parent strain with UV radiation and mutation Derivatives such as N-methyl-N′nitro-N-nitrosoguanidine (NTG), Having a resistance to 5-fluorotryptophan which enhances Biosynthesis of N5-N10-tetrahydrofolate used for transferring Two formyl group during the process of puring biosynthesis, making It possible to accumulate 5′xanthylic acid in culture medium at a high Yield and high concentration rate same period of fermentation.
摘要翻译： 本发明涉及通过用UV辐射和突变衍生物如N-甲基-N'-硝基-N-亚硝基胍（NTG）处理产生5'-辛酸作为亲本菌株的产氨基酸棒杆菌KCCM10488得到的微生物，具有抗 5-fluorotryptophan，其增强用于转移的N5-N10-四氢叶酸的生物合成在生物合成过程中两个甲酰基，使得在相同发酵时间的高收率和高浓度下可以在培养基中积累5'叶酸。
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公开(公告)号：US07456010B2
公开(公告)日：2008-11-25
申请号：US10531971
申请日：2003-12-10
申请人： Young-Hyeon Kwag , Ki-Hoon Oh , Jeong-Hwan Kim , Yoon-Suk Oh , Jae-Ick Sim , Young-Hoon Park , Jea-Young Chang
发明人： Young-Hyeon Kwag , Ki-Hoon Oh , Jeong-Hwan Kim , Yoon-Suk Oh , Jae-Ick Sim , Young-Hoon Park , Jea-Young Chang
IPC分类号： C12N1/20
CPC分类号： C12P17/182 , C12R1/15
摘要： The invention relates to Corynebacterium ammoniagenes CJXSP 0201 KCCM 10448 producing 5′-xanthylic acid. More specifically, the invention relates to Corynebacterium ammoniagenes CJXSP 0201 KCCM 10448 which is a mutant strain of Corynebacterium ammoniagenes KCCM 10340 having a resistance to osmotic pressure. In order to obtain mutant strain having enhanced respiratory activity, the present invention adopted Corynebacterium ammoniagenes KCCM 10340 as parent strain and treated it with UV radiation and mutation derivatives such as N-methyl-N′-nitro-n-nitrosoguanidine (NTG) according to ordinary procedure. Therefore, Corynebacterium ammoniagenes CJXSP 0201 KCCM 10448 of the present invention makes it possible to overcome growth-standing phase rapidly on early culture and for same period of fermentation, can accumulate 5′-xanthylic acid in culture medium at a high yield and concentration rate.
摘要翻译： 本发明涉及产生5'-戊酸的产氨棒杆菌CJXSP 0201 KCCM 10448。 更具体地，本发明涉及具有抗渗透压的耐氨基酸棒杆菌KCCM 10340的突变菌株的产氨棒杆菌CJXSP 0201 KCCM 10448。 为了获得具有增强的呼吸活性的突变菌株，本发明使用产氨杆菌KCCM 10340作为亲本菌株，并用UV辐射和N-甲基-N'-硝基-N-亚硝基胍（NTG）等突变衍生物按照 普通程序。 因此，本发明的产氨酸棒杆菌CJXSP 0201 KCCM 10448可以在早期培养中快速克服生长稳定期，并且在相同的发酵期内，可以以高产率和浓度的速率在培养基中积累5'-叶酸。
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公开(公告)号：US20060154346A1
公开(公告)日：2006-07-13
申请号：US10531675
申请日：2003-12-10
申请人： Young-Hyeon Kwag , Ki-Hoon Oh , Jeong-Hwan Kim , Yoon-Suk Oh , Jae-Ick Sim , Young-Hoon Park , Jea-Young Chang
发明人： Young-Hyeon Kwag , Ki-Hoon Oh , Jeong-Hwan Kim , Yoon-Suk Oh , Jae-Ick Sim , Young-Hoon Park , Jea-Young Chang
摘要： The invention relates to Corynebacterium ammoniagenes CJXOL 0201 KCCM 10447 producing 5′-xanthylic acid. More specifically, the invention relates to Corynebacterium ammoniagenes CJXOL 0201 KCCM 10447 which is a mutant strain of Corynebacterium ammoniagenes KCCM 10340 having a resistance to oligomycin. In order to obtain mutant strain having enhanced respiratory activity, the present invention adopted Corynebacterium ammoniagenes KCCM 10340 as parent strain and treated it with UV radiation and mutation derivatives such as N-methyl-N′-nitro-n-nitrosoguanidine(NTG) according to ordinary procedure. Therefore, Corynebacterium ammoniagenes CJXOL 0201 KCCM 10447 of the present invention makes it possible to increase ATP reproducing activity for same period of fermentation and can accumulate 5′xanthylic acid in culture medium at a high yield and concentration rate.
摘要翻译： 本发明涉及产生5'-戊酸的产氨棒杆菌CJXOL 0201 KCCM 10447。 更具体地，本发明涉及产氨杆菌CJXOL 0201 KCCM 10447，它是具有对寡霉素抗性的产氨棒杆菌KCCM10340的突变菌株。 为了获得具有增强的呼吸活性的突变菌株，本发明使用产氨杆菌KCCM 10340作为亲本菌株，并用UV辐射和N-甲基-N'-硝基-N-亚硝基胍（NTG）等突变衍生物按照 普通程序。 因此，本发明的产氨酸棒杆菌CJXOL 0201 KCCM 10447可以在相同的发酵期内增加ATP的再生活性，并且可以高产率和浓度的速率在培养基中积累5'-戊酸。
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4.发明申请MICROORGANISM PRODUCING GLUTAMIC ACID IN HIGH YIELD AND A PROCESS OF PRODUCING GLUTAMIC ACID USING THE SAME 有权在高产量下生产谷氨酸的微生物和使用其生产谷氨酸的方法公开(公告)号：US20110027840A1
公开(公告)日：2011-02-03
申请号：US12311871
申请日：2007-10-11
申请人： Chang hyun Jin , Ki-hoon Oh , Kwang Myung Cho , Young Hoon Park
发明人： Chang hyun Jin , Ki-hoon Oh , Kwang Myung Cho , Young Hoon Park
摘要： Disclosed herein are mutant strains, KCCM-10784P and KCCM-10785P, which are obtained through gene manipulation of Corynebacterium glutamicum KFCC-11074, and a process of producing L-glutamic acid using the mutant strains. The mutant strains are capable of producing L-Glutamic acid at high yield.
摘要翻译： 本文公开了通过谷氨酸棒杆菌KFCC-11074的基因操作获得的突变菌株KCCM-10784P和KCCM-10785P，以及使用突变菌株产生L-谷氨酸的方法。 突变株能以高产率生产L-谷氨酸。
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5.发明申请Escherichia Strain Capable of Converting Xmp to Gmp and Maintaining the Inactivated State of Gene(s) Associated with Gmp Degradation and Methods of Using the Same 有权能够将Xmp转化为Gmp并维持与Gmp降解有关的基因失活状态的大肠杆菌菌株及其使用方法公开(公告)号：US20080299620A1
公开(公告)日：2008-12-04
申请号：US11814417
申请日：2006-01-20
申请人： Young-hoon Park , Hyoung-suk Kim , Jin-nam Lee , Ki-hoon Oh , Jeong-hwan Kim , Yoon-suk Oh , Jae-ick Sim , Kyung-oh Choi
发明人： Young-hoon Park , Hyoung-suk Kim , Jin-nam Lee , Ki-hoon Oh , Jeong-hwan Kim , Yoon-suk Oh , Jae-ick Sim , Kyung-oh Choi
CPC分类号： C12N9/16 , C12N9/1077 , C12P19/32 , C12R1/19
摘要： Provided are mutant strains derived from Escherichia sp. GPU1114 (Accession No. KCCM-10536), having cumulative inactivation of deoD, aphA, appA, and hprt genes, and methods of using the same.
摘要翻译： 提供源自大肠杆菌属的突变菌株。 具有deoD，aphA，appA和hprt基因的累积失活的GPU1114（登录号KCCM-10536）及其使用方法。
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公开(公告)号：US07217558B2
公开(公告)日：2007-05-15
申请号：US10531675
申请日：2003-12-10
申请人： Young-Hyeon Kwag , Ki-Hoon Oh , Jeong-Hwan Kim , Yoon-Suk Oh , Jae-Ick Sim , Young-Hoon Park , Jea-Young Chang
发明人： Young-Hyeon Kwag , Ki-Hoon Oh , Jeong-Hwan Kim , Yoon-Suk Oh , Jae-Ick Sim , Young-Hoon Park , Jea-Young Chang
摘要： The invention relates to Corynebacterium ammoniagenes CJXOL 0201 KCCM 10447 producing 5′-xanthylic acid. More specifically, the invention relates to Corynebacterium ammoniagenes CJXOL 0201 KCCM 10447 which is a mutant strain of Corynebacterium ammoniagenes KCCM 10340 having a resistance to oligomycin. In order to obtain mutant strain having enhanced respiratory activity, the present invention adopted Corynebacterium ammoniagenes KCCM 10340 as parent strain and treated it with UV radiation and mutation derivatives such as N-methyl-N′-nitro-n-nitrosoguanidine(NTG) according to ordinary procedure. Therefore, Corynebacterium ammoniagenes CJXOL 0201 KCCM 10447 of the present invention makes it possible to increase ATP reproducing activity for same period of fermentation and can accumulate 5′xanthylic acid in culture medium at a high yield and concentration rate.
摘要翻译： 本发明涉及产生5'-戊酸的产氨棒杆菌CJXOL 0201 KCCM 10447。 更具体地，本发明涉及产氨杆菌CJXOL 0201 KCCM 10447，它是具有对寡霉素抗性的产氨棒杆菌KCCM10340的突变菌株。 为了获得具有增强的呼吸活性的突变菌株，本发明使用产氨杆菌KCCM 10340作为亲本菌株，并用UV辐射和N-甲基-N'-硝基-N-亚硝基胍（NTG）等突变衍生物按照 普通程序。 因此，本发明的产氨酸棒杆菌CJXOL 0201 KCCM 10447可以在相同的发酵期内增加ATP的再生活性，并且可以以高产率和浓度的速率在培养基中积累5'-戊酸。
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7.发明授权Microorganism producing glutamic acid in high yield and a process of producing glutamic acid using the same 有权以高产率生产谷氨酸的微生物和使用其生产谷氨酸的方法公开(公告)号：US07955823B2
公开(公告)日：2011-06-07
申请号：US12311871
申请日：2007-10-11
申请人： Chang hyun Jin , Ki-Hoon Oh , Kwang Myung Cho , Young Hoon Park
发明人： Chang hyun Jin , Ki-Hoon Oh , Kwang Myung Cho , Young Hoon Park
摘要： Disclosed herein are mutant strains, KCCM-10784P and KCCM-10785P, which are obtained through gene manipulation of Corynebacterium glutamicum KFCC-11074, and a process of producing L-glutamic acid using the mutant strains. The mutant strains are capable of producing L-Glutamic acid at high yield.
摘要翻译： 本文公开了通过谷氨酸棒杆菌KFCC-11074的基因操作获得的突变菌株KCCM-10784P和KCCM-10785P，以及使用突变菌株产生L-谷氨酸的方法。 突变株能以高产率生产L-谷氨酸。
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公开(公告)号：US20070141682A1
公开(公告)日：2007-06-21
申请号：US10582166
申请日：2004-11-18
申请人： Young-Hoon Park , Jea-Young Chang , Jin-Nam Lee , Ki-Hoon Oh , Jeong-Hwan Kim , Yoon-Suk Oh , Jae-Ick Sim
发明人： Young-Hoon Park , Jea-Young Chang , Jin-Nam Lee , Ki-Hoon Oh , Jeong-Hwan Kim , Yoon-Suk Oh , Jae-Ick Sim
CPC分类号： C12P19/32 , C12R1/15 , Y10S435/822 , Y10S435/843
摘要： The invention relates to a microorganism, obtained by treating Corynebacterium ammoniagenes KCCM 10488 producing 5′-Xanthylic acid as parent strain with UV radiation and mutation Derivatives such as N-methyl-N′nitro-N-nitrosoguanidine (NTG), Having a resistance to 5-fluorotryptophan which enhances Biosynthesis of N5-N10-tetrahydrofolate used for transferring Two formyl group during the process of puring biosynthesis, making It possible to accumulate 5′xanthylic acid in culture medium at a high Yield and high concentration rate same period of fermentation.
摘要翻译： 本发明涉及通过用UV辐射和突变衍生物如N-甲基-N'-硝基-N-亚硝基胍（NTG）处理产生5'-辛酸作为亲本菌株的产氨基酸棒杆菌KCCM10488得到的微生物，具有抗 5-fluorotryptophan，其增强用于转移的N5-N10-四氢叶酸的生物合成在生物合成过程中两个甲酰基，使得在相同发酵时间的高收率和高浓度下可以在培养基中积累5'叶酸。
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公开(公告)号：US08669078B2
公开(公告)日：2014-03-11
申请号：US12097104
申请日：2006-12-14
申请人： Jae-Gu Pan , Heung-Chae Jung , Eui-Joong Kim , Han-Seung Lee , Young Hoon Park , Hyoung Suk Kim , Jong-Kwon Han , Jin Nam Lee , Ki-Hoon Oh , Jeong Hwan Kim , Yoon-Suk Oh , Jae Ick Sim , Kuk-Ki Hong , Kyung Oh Choi , Hyun Soo Kim , Min-Ji Baek , Tae Sun Kang
发明人： Jae-Gu Pan , Heung-Chae Jung , Eui-Joong Kim , Han-Seung Lee , Young Hoon Park , Hyoung Suk Kim , Jong-Kwon Han , Jin Nam Lee , Ki-Hoon Oh , Jeong Hwan Kim , Yoon-Suk Oh , Jae Ick Sim , Kuk-Ki Hong , Kyung Oh Choi , Hyun Soo Kim , Min-Ji Baek , Tae Sun Kang
摘要： Disclosed herein are ammonia-specific 5′-XMP aminase mutants and a method for preparing the same. A mutation is introduced into the active site of glutamine-dependent catalysis in 5′-XMP aminase. The resulting 5′-XMP aminase mutant is devoid of the glutamine-dependent activity and specifically reacts with external ammonia in converting 5′-XMP into 5′-GMP. Thus, the ammonia-specific 5′-XMP aminase mutant is stabler within cells compared to the wild type, and can be useful in the industrial conversion of 5′-XMP into 5′-GMP.
摘要翻译： 本文公开了氨特异性5'-XMP氨基酶突变体及其制备方法。 在5'-XMP氨基酶中引入谷氨酰胺依赖性催化活性位点的突变。 所得到的5'-XMP氨基酶突变体缺乏谷氨酰胺依赖性活性，并特异性地与外部氨反应将5'-XMP转化为5'-GMP。 因此，与野生型相比，氨特异性5'-XMP氨基酶突变体在细胞内更稳定，可用于将5'-XMP工业转化为5'-GMP。
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公开(公告)号：US20080318278A1
公开(公告)日：2008-12-25
申请号：US12097104
申请日：2006-12-14
申请人： Jae-Gu Pan , Heung-Chae Jung , Eui-Joong Kim , Han-Seung Lee , Young Hoon Park , Hyoung Suk Kim , Jong-Kwon Han , Jin Nam Lee , Ki-Hoon Oh , Jeong Hwan Kim , Yoon-Suk Oh , Jae Ick Sim , Kuk-Ki Hong , Kyung Oh Choi , Hyun Soo Kim , Min-Ji Baek , Tae Sun Kang
发明人： Jae-Gu Pan , Heung-Chae Jung , Eui-Joong Kim , Han-Seung Lee , Young Hoon Park , Hyoung Suk Kim , Jong-Kwon Han , Jin Nam Lee , Ki-Hoon Oh , Jeong Hwan Kim , Yoon-Suk Oh , Jae Ick Sim , Kuk-Ki Hong , Kyung Oh Choi , Hyun Soo Kim , Min-Ji Baek , Tae Sun Kang
摘要： Disclosed herein are ammonia-specific 5′-XMP aminase mutants and a method for preparing the same. A mutation is introduced into the active site of glutamine-dependent catalysis in 5′-XMP aminase. The resulting 5′-XMP aminase mutant is devoid of the glutamine-dependent activity and specifically reacts with external ammonia in converting 5′-XMP into 5′-GMP. Thus, the ammonia-specific 5′-XMP aminase mutant is stabler within cells compared to the wild type, and can be useful in the industrial conversion of 5′-XMP into 5′-GMP.
摘要翻译： 本文公开了氨特异性5'-XMP氨基酶突变体及其制备方法。 在5'-XMP氨基酶中引入谷氨酰胺依赖性催化活性位点的突变。 所得到的5'-XMP氨基酶突变体缺乏谷氨酰胺依赖性活性，并特异性地与外部氨反应将5'-XMP转化为5'-GMP。 因此，与野生型相比，氨特异性5'-XMP氨基酶突变体在细胞内更稳定，可用于将5'-XMP工业转化为5'-GMP。
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