公开(公告)号：US09187774B2

公开(公告)日：2015-11-17

申请号：US13581287

申请日：2011-02-25

申请人： James C. Liao ,  Kechun Zhang ,  Kwang Myung Cho

发明人： James C. Liao ,  Kechun Zhang ,  Kwang Myung Cho

IPC分类号： C12P13/04 ,  C12N9/06

CPC分类号： C12N9/0016 ,  C12P13/04 ,  C12P13/06 ,  C12Y104/01002 ,  Y02P20/52

摘要： Healthcare costs are a significant worldwide, with many patients being denied medications because of their high prices. One approach to addressing this problem involves the biosynthesis of chiral drug intermediates, an environmentally friendly solution that can be used to generate pharmaceuticals at much lower costs than conventional techniques. In this context, embodiments of the invention comprise methods and materials designed to allow microorganisms to biosynthesize the nonnatural amino acid L-homoalanine. As is known in the art, L-homoalanine is a chiral precursor of a variety of pharmaceutically valuable compounds including the anticonvulsant medications levetiracetam (sold under the trade name Keppra®) and brivaracetam, as well as ethambutol, a bacteriostatic antimycobacterial drug used to treat tuberculosis. Consequently, embodiments of the invention can be used in low cost, environmentally friendly processes to generate these and other valuable compounds.

摘要翻译： 保健费用在世界范围内是重要的，许多患者由于价格高昂而被拒绝服用药物。 解决这一问题的一种方法涉及手性药物中间体的生物合成，这是一种环境友好的解决方案，可以以比传统技术低得多的成本生产药物。 在本文中，本发明的实施方案包括设计用于允许微生物生物合成非天然氨基酸L-高丙氨酸的方法和材料。 如本领域已知的，L-高丙氨酸是各种药学上有价值的化合物的手性前体，包括抗惊厥药物左乙拉西坦（商品名为Keppra）和布立西坦（brivaracetam）），以及乙胺丁醇，用于治疗的抑菌抗分支杆菌药物 结核。 因此，本发明的实施方案可以用于低成本，环境友好的方法以产生这些和其它有价值的化合物。

公开(公告)号：US09150889B2

公开(公告)日：2015-10-06

申请号：US13522288

申请日：2011-01-15

申请人： James C. Liao ,  Kwang Myung Cho

发明人： James C. Liao ,  Kwang Myung Cho

IPC分类号： C12P7/22 ,  C12P7/16 ,  C12N9/04 ,  C12N9/88 ,  C12P7/04

CPC分类号： C12P7/22 ,  C12N9/0006 ,  C12N9/88 ,  C12P7/04 ,  C12P7/16 ,  C12Y101/01001 ,  C12Y401/01072 ,  Y02E50/10 ,  Y02P20/134 ,  Y02P20/52 ,  Y02P20/59

摘要： The disclosure provides a process that converts CO2 to higher alcohols (e.g. isobutanol) using electricity as the energy source. This process stores electricity (e.g. from solar energy, nuclear energy, and the like) in liquid fuels that can be used as high octane number gasoline substitutes. Instead of deriving reducing power from photosynthesis, this process derives reducing power from electrically generated mediators, either H2 or formate. H2 can be derived from electrolysis of water. Formate can be generated by electrochemical reduction of CO2. After delivering the reducing power in the cell, formate becomes CO2 and recycles back. Therefore, the biological CO2 fixation process can occur in the dark.

摘要翻译： 本公开提供了使用电作为能量源将CO 2转化为高级醇（例如异丁醇）的方法。 该方法在可用作高辛烷值汽油替代物的液体燃料中储存电力（例如来自太阳能，核能等）。 不是从光合作用中获得降低功率，而是从电生成的介质（H2或甲酸盐）中减少能量。 H2可以从电解水中得到。 甲酸可以通过电化学还原二氧化碳产生。 在细胞中提供还原能力后，甲酸成为二氧化碳并循环回收。 因此，生物二氧化碳固定过程可能发生在黑暗中。

公开(公告)号：US20130288325A1

公开(公告)日：2013-10-31

申请号：US13883531

申请日：2011-11-03

申请人： James C. Liao ,  Kwang Myung Cho ,  Yajun Yan ,  Yixin Huo

发明人： James C. Liao ,  Kwang Myung Cho ,  Yajun Yan ,  Yixin Huo

IPC分类号： C12N15/81 ,  C12N15/77 ,  C12N15/78 ,  C12N15/75 ,  C12N15/63 ,  C12N15/80

CPC分类号： C12N15/815 ,  C12N1/20 ,  C12N9/0016 ,  C12N9/1096 ,  C12N15/63 ,  C12N15/75 ,  C12N15/77 ,  C12N15/78 ,  C12N15/80 ,  C12N15/81 ,  C12P7/04 ,  C12P7/16 ,  C12P7/24 ,  C12P7/40 ,  C12P13/04 ,  Y02E50/10 ,  Y02E50/17 ,  Y02P20/582

摘要： Provided herein are metabolically modified microorganisms characterized by having an increased keto-acid flux when compared with the wild-type organism and comprising at least one polynucleotide encoding an enzyme that when expressed results in the production of a greater quantity of a chemical product when compared with the wild-type organism. The recombinant microorganisms are useful for producing a large number of chemical compositions from various nitrogen containing biomass compositions and other carbon sources. More specifically, provided herein are methods of producing alcohols, acetaldehyde, acetate, isobutyraldehyde, isobutyric acid, n-butyraldehyde, n-butyric acid, 2-methyl-1-butyraldehyde, 2-methyl-1-butyric acid, 3-methyl-1-butyraldehyde, 3-methyl-1-butyric acid, ammonia, ammonium, amino acids, 2,3-butanediol, 1,4-butanediol, 2-methyl-1,4-butanediol, 2-methyl-1,4-butanediamine, isobutene, itaconate, acetoin, acetone, isobutene, 1,5-diaminopentane, L-lactic acid, D-lactic acid, shikimic acid, mevalonate, polyhydroxybutyrate (PHB), isoprenoids, fatty acids, homoalanine, 4-aminobutyric acid (GABA), succinic acid, malic acid, citric acid, adipic acid, p-hydroxy-cinnamic acid, tetrahydrofuran, 3-methyl-tetrahydrofuran, gamma-butyrolactone, pyrrolidinone, n-methylpyrrolidone, aspartic acid, lysine, cadeverine, 2-ketoadipic acid, and/or S-adenosyl-methionine (SAM) from a suitable nitrogen rich biomass.

摘要翻译： 本文提供了代谢修饰的微生物，其特征在于与野生型生物体相比具有增加的酮酸通量，并且包含至少一种编码酶的多核苷酸，当表达导致产生更大量的化学产品时，与 野生型生物体。 重组微生物可用于从各种含氮生物质组合物和其它碳源生产大量化学成分。 更具体地，本文提供了生产醇，乙醛，乙酸酯，异丁醛，异丁酸，正丁醛，正丁酸，2-甲基-1-丁醛，2-甲基-1-丁酸，3-甲基-1-丁酸， 1-丁醛，3-甲基-1-丁酸，氨，铵，氨基酸，2,3-丁二醇，1,4-丁二醇，2-甲基-1,4-丁二醇，2-甲基-1,4-丁二醇， 丁二胺，异丁烯，衣康酸酯，乙偶姻，丙酮，异丁烯，1,5-二氨基戊烷，L-乳酸，D-乳酸，莽草酸，甲羟戊酸，聚羟基丁酸酯（PHB），类异戊二烯，脂肪酸，高丙氨酸，4-氨基丁酸 GABA），琥珀酸，苹果酸，柠檬酸，己二酸，对羟基肉桂酸，四氢呋喃，3-甲基 - 四氢呋喃，γ-丁内酯，吡咯烷酮，正 - 甲基吡咯烷酮，天冬氨酸，赖氨酸，仙茅素，2-酮己二酸 酸和/或S-腺苷 - 甲硫氨酸（SAM）。

公开(公告)号：US07847134B2

公开(公告)日：2010-12-07

申请号：US12594025

申请日：2009-02-27

申请人： Jung Ho Lee ,  Jong San Chang ,  Young Kyu Hwang ,  Hyun Kwan Shin ,  Kwang Myung Cho ,  Bong Keun Song ,  Yong Hwan Kim

发明人： Jung Ho Lee ,  Jong San Chang ,  Young Kyu Hwang ,  Hyun Kwan Shin ,  Kwang Myung Cho ,  Bong Keun Song ,  Yong Hwan Kim

IPC分类号： C07C29/147

CPC分类号： C07C29/149 ,  B01J23/626 ,  B01J23/6567 ,  B01J23/8966 ,  B01J37/0009 ,  B01J37/031 ,  B01J37/10 ,  B01J37/18 ,  B01J2523/00 ,  C07C31/12 ,  B01J2523/27 ,  B01J2523/43 ,  B01J2523/821 ,  B01J2523/74 ,  B01J2523/828 ,  B01J2523/845 ,  B01J2523/17 ,  B01J2523/47 ,  B01J2523/41

摘要： Disclosed herein is a method for producing monohydric alcohols from monocarboxylic acids or derivatives thereof using a catalyst comprising ruthenium (Ru) and tin (Sn) using zinc oxide (ZnO) as both a catalyst support and an active promoter; a catalyst prepared by adding an inorganic binder such as silica, alumina or titania in a limited range to the catalyst comprising the above components in order to impart a shaping ability to the catalyst; or, a modified catalyst reformed by adding at least one reducing component selected from the group consisting of Co, Ni, Cu, Ag, Rh, Pd, Re, Ir, and Pt to the catalyst in order to improve the reducing ability of the catalyst. By using such catalysts, the method according to the present invention is advantageous in that the monohydric alcohols can be prepared in high yield regardless of whether the monocarboxylic acids contain water or not, the monohydric alcohols can be economically prepared because the catalysts can be operated under mild reaction conditions and also exhibits high selectivity and productivity compared to conventional catalysts, and the catalysts have excellent long-term reaction stability so as to be advantageous for industrial applications.

摘要翻译： 本文公开了使用氧化锌（ZnO）作为催化剂载体和活性促进剂两者，使用包含钌（Ru）和锡（Sn）的催化剂由一元羧酸或其衍生物生产一元醇的方法; 通过在包含上述组分的催化剂的有限范围内加入无机粘合剂如二氧化硅，氧化铝或二氧化钛制备的催化剂，以赋予催化剂成型能力; 或者，通过向催化剂中添加选自Co，Ni，Cu，Ag，Rh，Pd，Re，Ir和Pt中的至少一种还原成分而改性的改性催化剂，以提高催化剂的还原能力 。 通过使用这样的催化剂，根据本发明的方法的优点在于，一元醇可以以高产率制备，而不管单羧酸是否含有水，一元醇可经济地制备，因为催化剂可以在 温和的反应条件，并且与常规催化剂相比表现出高选择性和生产率，并且催化剂具有优异的长期反应稳定性，从而有利于工业应用。

公开(公告)号：US20100081173A1

公开(公告)日：2010-04-01

申请号：US12522004

申请日：2008-01-15

申请人： Young-hoon Park ,  Kwang-myung Cho ,  Hee-jong Lee ,  Jin-nam Lee

发明人： Young-hoon Park ,  Kwang-myung Cho ,  Hee-jong Lee ,  Jin-nam Lee

IPC分类号： C12P19/40 ,  C12N1/21

CPC分类号： C12P19/32 ,  C12N9/16 ,  C12N9/2497 ,  C12P19/40 ,  C12Y302/02008

摘要： The present invention relates to a microorganism producing inosine, which is one of purine nucleoside, an important material for 5′-inosinic acid synthesis, and method for producing inosine using the same. More particularly, the present invention relates to a recombinant microorganism of Corynebacterium genus producing inosine at high concentration by inactivating the gene encoding nucleoside hydrolase II and by enhancing the expression of the gene encoding 5′-nucleotidase, which still retains the characteristics of Corynebacterium ammoniagenes CJIP2401 (KCCM-10610).

摘要翻译： 本发明涉及一种生产肌苷的微生物，其是嘌呤核苷之一，是5'-肌苷酸合成的重要材料，以及使用其的产生肌苷的方法。 更具体地说，本发明涉及通过使编码核苷水解酶II的基因失活而产生高浓度肌苷的棒杆菌属的重组微生物，并且通过增强仍保留产氨棒杆菌CJIP2401的特征的编码5'-核苷酸酶的基因的表达， （KCCM-10610）。

公开(公告)号：US09777297B2

公开(公告)日：2017-10-03

申请号：US14356386

申请日：2012-11-02

申请人： Kwang Myung Cho ,  Wendy Higashide ,  Chrissie Lee ,  Shahrooz Rabizadeh

发明人： Kwang Myung Cho ,  Wendy Higashide ,  Chrissie Lee ,  Shahrooz Rabizadeh

IPC分类号： C12P7/16 ,  C12P7/24 ,  C12N15/52 ,  C12N1/21 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N15/52 ,  C12P7/24 ,  C12Y101/01157 ,  C12Y102/01057 ,  C12Y103/01086 ,  C12Y203/01009

摘要： Microorganisms and methods of producing n-butyraldehyde with enhanced yields are presented in which a microorganism is engineered to enhance the conversion of a carbon source into n-butyraldehyde. The n-butyraldehyde is recovered by way of a gas stripping process that occurs during the conversion process, providing significantly greater product yield than post-fermentation recovery of n-butyraldehyde alone.

公开(公告)号：US08426171B2

公开(公告)日：2013-04-23

申请号：US12066111

申请日：2007-07-30

申请人： So-young Kim ,  Kwang-myung Cho ,  Yong-uk Shin ,  Hye-won Um ,  Kyung-oh Choi ,  Jin-sook Chang ,  Young-wook Cho ,  Young-hoon Park

发明人： So-young Kim ,  Kwang-myung Cho ,  Yong-uk Shin ,  Hye-won Um ,  Kyung-oh Choi ,  Jin-sook Chang ,  Young-wook Cho ,  Young-hoon Park

IPC分类号： C12P13/12 ,  C12P1/00 ,  C12P21/04

CPC分类号： C12P13/12 ,  C07C323/58 ,  C12N9/1029 ,  C12N9/1085 ,  C12P7/46 ,  C12P13/06

摘要： The present invention relates to a method for producing L-methionine and organic acid comprising the following steps: Step 1) preparing a strain producing L-methionine precursor and producing L-methionine precursor by the fermentation of the strain; Step 2) producing L-methionine and organic acid by the enzyme reaction process with the L-methionine precursor as a substrate, and microorganism strains used in each step.

摘要翻译： 本发明涉及L-甲硫氨酸和有机酸的制造方法，其特征在于，包括以下工序：工序1）制备生产L-甲硫氨酸前体的菌株，通过菌株的发酵生产L-甲硫氨酸前体; 步骤2）通过与L-甲硫氨酸前体作为底物的酶反应方法生产L-甲硫氨酸和有机酸，以及每个步骤中使用的微生物菌株。

公开(公告)号：US08426165B2

公开(公告)日：2013-04-23

申请号：US12522304

申请日：2008-01-22

申请人： Kwang-myung Cho ,  Kyung-oh Choi ,  Hyun-ae Bae

发明人： Kwang-myung Cho ,  Kyung-oh Choi ,  Hyun-ae Bae

IPC分类号： C12N15/00

CPC分类号： C12P13/14 ,  C12N9/0006 ,  C12N9/1205 ,  C12P13/08 ,  C12Y101/05003 ,  C12Y207/0103

摘要： The present invention relates to a method for producing fermentation product from various carbon sources containing glycerol using Corynebacteria. More particularly, the present invention relates to a method for producing fermentation product from carbon sources containing glycerol or a part of glycerol with high yield and high productivity, by fermenting Corynebacteria introduced with the foreign gene glpDFK facilitating the use of glycerol and accumulating industrially useful amino acids in the culture medium.

摘要翻译： 本发明涉及使用棒杆菌从含有甘油的各种碳源生产发酵产物的方法。 更具体地说，本发明涉及通过发酵引入外源基因glpDFK的棒状杆菌来促进甘油的使用并累积工业上有用的氨基，从含有甘油或一部分甘油的甘油或一部分甘油产生发酵产物的方法 培养基中的酸。

公开(公告)号：US08168422B2

公开(公告)日：2012-05-01

申请号：US12522004

申请日：2008-01-15

申请人： Young-hoon Park ,  Kwang-myung Cho ,  Hee-jong Lee ,  Jin-nam Lee

发明人： Young-hoon Park ,  Kwang-myung Cho ,  Hee-jong Lee ,  Jin-nam Lee

IPC分类号： C12P19/40 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12P19/32 ,  C12N9/16 ,  C12N9/2497 ,  C12P19/40 ,  C12Y302/02008

摘要： The present invention relates to a microorganism producing inosine, which is one of purine nucleoside, an important material for 5′-inosinic acid synthesis, and method for producing inosine using the same. More particularly, the present invention relates to a recombinant microorganism of Corynebacterium genus producing inosine at high concentration by inactivating the gene encoding nucleoside hydrolase II and by enhancing the expression of the gene encoding 5′-nucleotidase, which still retains the characteristics of Corynebacterium ammoniagenes CJIP2401 (KCCM-10610).

摘要翻译： 本发明涉及一种生产肌苷的微生物，其是嘌呤核苷之一，是5'-肌苷酸合成的重要材料，以及使用其的产生肌苷的方法。 更具体地说，本发明涉及通过使编码核苷水解酶II的基因失活而产生高浓度肌苷的棒杆菌属的重组微生物，并且通过增强仍保留产氨棒杆菌CJIP2401的特征的编码5'-核苷酸酶的基因的表达， （KCCM-10610）。

公开(公告)号：US20110318783A1

公开(公告)日：2011-12-29

申请号：US13229166

申请日：2011-09-09

申请人： Young-hoon Park ,  Kwang-myung Cho ,  Hee-jong Lee ,  Jin-nam Lee

发明人： Young-hoon Park ,  Kwang-myung Cho ,  Hee-jong Lee ,  Jin-nam Lee

IPC分类号： C12P19/40

CPC分类号： C12P19/32 ,  C12N9/16 ,  C12N9/2497 ,  C12P19/40 ,  C12Y302/02008

摘要： The present invention relates to a microorganism producing inosine, which is one of purine nucleoside, an important material for 5′-inosinic acid synthesis, and method for producing inosine using the same. More particularly, the present invention relates to a recombinant microorganism of Corynebacterium genus producing inosine at high concentration by inactivating the gene encoding nucleoside hydrolase II and by enhancing the expression of the gene encoding 5′-nucleotidase, which still retains the characteristics of Corynebacterium ammoniagenes CJIP2401 (KCCM-10610).

摘要翻译： 本发明涉及一种生产肌苷的微生物，其是嘌呤核苷之一，是5'-肌苷酸合成的重要材料，以及使用其的产生肌苷的方法。 更具体地说，本发明涉及通过使编码核苷水解酶II的基因失活而产生高浓度肌苷的棒杆菌属的重组微生物，并且通过增强仍保留产氨棒杆菌CJIP2401的特征的编码5'-核苷酸酶的基因的表达， （KCCM-10610）。