公开(公告)号：US5795791A

公开(公告)日：1998-08-18

申请号：US780286

申请日：1997-01-08

申请人： Kikuo Hirai ,  Yoshihiko Makino

发明人： Kikuo Hirai ,  Yoshihiko Makino

IPC分类号： G01N33/52 ,  G01N33/48 ,  G01N33/53 ,  G01N33/96 ,  G01N35/00 ,  G01N33/566

CPC分类号： G01N33/96 ,  G01N33/48 ,  Y10S435/967

摘要： One sigmoid calibration curve is split into three parts of low concentration region represented by a high degree function, intermediate concentration region represented by an exponential function and high concentration region represented by a high degree function according to the present invention. The boundary condition of the adjacent two functions is set so that the two functions have an equal slope at the boundary point; thereby, regression functions of the calibration curves in respective regions are found. The number of standard samples for finding a calibration curve can be reduced while the calibration curve found is of high accuracy.

公开(公告)号：US5948368A

公开(公告)日：1999-09-07

申请号：US947658

申请日：1997-10-09

申请人： Kikuo Hirai ,  Yoshihiko Makino

发明人： Kikuo Hirai ,  Yoshihiko Makino

IPC分类号： G01N33/52 ,  G01N33/48 ,  G01N33/53 ,  G01N33/96 ,  G01N35/00

CPC分类号： G01N33/96 ,  G01N33/48 ,  Y10S435/967

摘要： One sigmoid calibration curve is split into three parts of low concentration region represented by a high degree function, intermediate concentration region represented by an exponential function and high concentration region represented by a high degree function according to the present invention. The boundary condition of the adjacent two functions is set so that the two functions have an equal slope at the boundary point; thereby, regression functions of the calibration curves in respective regions are found. The number of standard samples for finding a calibration curve can be reduced while the calibration curve found is of high accuracy.

摘要翻译： 一个S形校准曲线被分成由高度函数表示的低浓度区域三个部分，由指数函数表示的中间浓度区域和根据本发明的高度函数表示的高浓度区域。 相邻两个函数的边界条件被设置为使得两个函数在边界点具有相等的斜率; 从而发现各个区域的校准曲线的回归函数。 可以减少用于找到校准曲线的标准样品的数量，同时发现校准曲线具有高精度。

公开(公告)号：US5882935A

公开(公告)日：1999-03-16

申请号：US544133

申请日：1995-10-17

申请人： Kikuo Hirai ,  Hiroshi Shinoki ,  Masashi Ogawa ,  Yoshihiko Makino

发明人： Kikuo Hirai ,  Hiroshi Shinoki ,  Masashi Ogawa ,  Yoshihiko Makino

IPC分类号： G01N33/53 ,  G01N33/52 ,  G01N33/542 ,  G01N33/72 ,  H01L21/302 ,  H01L21/304 ,  H01L21/306 ,  H01L21/76

CPC分类号： G01N33/723 ,  G01N33/721

摘要： An analysis element for analyzing both amounts of glycated hemoglobin and total hemoglobin in an aqueous liquid sample to determine glycated hemoglobin content ratio in the sample. The element comprises a substrate layer for receiving a reaction mixture after the completion of an immunological reaction between the glycated hemoglobin in the sample and an enzyme-labelled antibody against the glycated hemoglobin, and a reagent layer. The substrate layer contains a non-diffusible substrate which forms a diffusible material in the presence of the enzyme of the enzyme-labelled antibody, the activity of the enzyme being effected relative to the steric hindrance due to the immunological reaction. The reagent layer contains a reagent composition for reacting with the diffusible material to form a dye detectable colorimetrically in a wavelength range which is not effected by an absorption spectrum of the hemoglobin. The total hemoglobin retained in the substrate layer and the dye formed in the reagent layer are colorimetrically analyzed separately, and the glycated hemoglobin content ratio is calculated from respective values. An analysis method using this analysis element is also provided.

摘要翻译： 分析元素，用于分析含水液体样品中糖化血红蛋白和总血红蛋白的量，以测定样品中糖化血红蛋白含量的比例。 该元件包括用于在样品中的糖化血红蛋白和糖化血红蛋白的酶标记抗体完成后的反应混合物和试剂层的基底层。 底物层含有在酶标记抗体的酶存在下形成可扩散材料的非扩散性底物，该酶的活性相对于由于免疫反应引起的空间位阻影响。 试剂层含有用于与可扩散材料反应的试剂组合物，以形成可在不受血红蛋白的吸收光谱影响的波长范围内比色测定的染料。 分别保留在基材层中的总血红蛋白和形成在试剂层中的染料，并根据各值计算糖化血红蛋白含量比。 还提供了使用该分析元素的分析方法。

公开(公告)号：US20060147991A1

公开(公告)日：2006-07-06

申请号：US11377058

申请日：2006-03-16

申请人： Hiroshi Shinoki ,  Yoshihiko Makino ,  Yumiko Takeshita ,  Junichi Yamanouchi ,  Yukio Sudo ,  Osamu Seshimoto

发明人： Hiroshi Shinoki ,  Yoshihiko Makino ,  Yumiko Takeshita ,  Junichi Yamanouchi ,  Yukio Sudo ,  Osamu Seshimoto

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C08G63/91 ,  C12M1/34

CPC分类号： B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00533 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00711 ,  B01J2219/00722 ,  B82Y30/00 ,  C07B2200/11 ,  C07C317/08 ,  C07H21/00 ,  C40B40/00

摘要： A reactive solid carrier favorably employable for manufacturing DNA chip is composed of a solid carrier and a plurality of vinylsulfonyl groups or their reactive precursors each of which is fixed onto a surface of the solid carrier by covalent bonding via a linking group, and a method for producing a DNA chip is performed by bringing the reactive solid carrier into contact with nucleotide derivatives or their analogues having a reactive group which is reactive with the vinylsulfonyl group or reactive precursor fixed to the solid carrier.

公开(公告)号：US20050266450A1

公开(公告)日：2005-12-01

申请号：US11106612

申请日：2005-04-15

申请人： Yoshihiko Makino ,  Toshihiro Mori ,  Yoshihide Iwaki

发明人： Yoshihiko Makino ,  Toshihiro Mori ,  Yoshihide Iwaki

IPC分类号： C12P19/34 ,  C12Q1/42 ,  C12Q1/68 ,  G01N33/52

CPC分类号： G01N33/523 ,  C12Q1/42 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2565/625 ,  C12Q2565/301 ,  C12Q2521/543 ,  C12Q2533/101

摘要： An object of the present invention is to provide a method for analyzing a target nucleic acid fragment which can be simply and swiftly carried out by using a small apparatus, a kit for analyzing a target nucleic acid fragment using the method for analysis, and a dry analytical element for quantifying pyrophosphoric acid. The present invention provides a method for analyzing pyrophosphoric acid generated upon polymerase elongation reaction based on certain nucleotide sequence of a target nucleic acid, a kit for analysis for carrying out the above mentioned method for analysis, and a dry analytical element for quantifying pyrophosphoric acid.

摘要翻译： 本发明的目的是提供一种用于分析目标核酸片段的方法，其可以通过使用小型装置简单快速地进行，使用该分析方法分析靶核酸片段的试剂盒和干燥的 用于定量焦磷酸的分析元素。 本发明提供一种基于靶核酸的某些核苷酸序列分析聚合酶延伸反应产生的焦磷酸的方法，用于进行上述分析方法的分析用试剂盒和定量焦磷酸的干燥分析元素。

公开(公告)号：US08511482B2

公开(公告)日：2013-08-20

申请号：US11630135

申请日：2005-09-15

申请人： Hideaki Tanaka ,  Yoshihiko Makino

发明人： Hideaki Tanaka ,  Yoshihiko Makino

IPC分类号： B01D71/16 ,  B01D39/16 ,  B01D39/14 ,  B01D71/12 ,  C02F1/44

CPC分类号： B01D67/0013 ,  B01D15/00 ,  B01D15/08 ,  B01D67/0016 ,  B01D67/0093 ,  B01D71/12 ,  B01D2323/08 ,  B01D2323/22 ,  B01D2325/02 ,  B01D2325/022 ,  B01D2325/12 ,  B01J20/26 ,  B01J20/265 ,  B01J20/28033 ,  C08J9/28

摘要： A method of producing a porous membrane, the method comprising: casting a polymer solution, in which a polymer is dissolved in a mixture of a good solvent, a poor solvent and a non-solvent, over a support, so as to form a casted polymer solution; drying the casted polymer solution, so as to form a cast film; and subjecting the cast film to a phase separation, wherein the porous membrane is produced under a condition where a temperature of a casted surface is lower than a temperature of the polymer solution, and each of a temperature change of the polymer solution and a temperature change of the casted surface is kept within ±3.0° C.

摘要翻译： 一种多孔膜的制造方法，其特征在于，在载体上将聚合物溶解在良溶剂，不良溶剂和非溶剂的混合物中的聚合物溶液中，形成铸塑 聚合物溶液; 干燥铸塑聚合物溶液，以形成流延膜; 并对流延膜进行相分离，其中在浇铸表面的温度低于聚合物溶液的温度的条件下制备多孔膜，并且聚合物溶液的温度变化和温度变化 的铸造表面保持在±3.0℃

公开(公告)号：US07572578B2

公开(公告)日：2009-08-11

申请号：US10568101

申请日：2004-09-08

申请人： Toshihiro Mori ,  Yoshihiko Makino ,  Rie Hando ,  Yumiko Takeshita ,  Hiroko Inomata

发明人： Toshihiro Mori ,  Yoshihiko Makino ,  Rie Hando ,  Yumiko Takeshita ,  Hiroko Inomata

IPC分类号： C12Q1/68

CPC分类号： C12N15/1006 ,  C12N15/1017

摘要： The present invention is a method for isolating and purifying a nucleic acid, where generation of foams is able to be suppressed whereby the isolation and purification of a nucleic acid are easily and efficiently carried out, the method for isolating and purifying a nucleic acid comprising the step of: (1) contacting a sample solution containing nucleic acid to a solid phase to adsorb the nucleic acid onto the solid phase; (2) contacting a washing solution to the solid phase to wash the solid phase in such a state that the nucleic acid is adsorbed; and (3) contacting an elution solution to the solid phase to desorb the nucleic acid, wherein the sample solution containing nucleic acid contains an antifoaming agent.

摘要翻译： 本发明是分离和纯化核酸的方法，其中能够抑制泡沫的产生，从而容易且有效地进行核酸的分离和纯化，分离和纯化包含 步骤：（1）将含有核酸的样品溶液与固相接触以将核酸吸附到固相上; （2）将洗涤溶液与固相接触以在核酸被吸附的状态下洗涤固相; 和（3）使洗脱溶液与固相接触以解吸核酸，其中含有核酸的样品溶液含有消泡剂。

公开(公告)号：US20050277152A1

公开(公告)日：2005-12-15

申请号：US11155469

申请日：2005-06-20

申请人： Yoshihiko Makino ,  Toshihiro Mori

发明人： Yoshihiko Makino ,  Toshihiro Mori

IPC分类号： G01N30/00 ,  C07H21/00 ,  C12M1/00 ,  C12N15/09 ,  C12N15/10 ,  C12Q1/68 ,  C12M1/34

CPC分类号： C12N15/1006 ,  C12Q1/6806 ,  C12Q2565/137

摘要： An object of the present invention is to provide a method for separation and purification of nucleic acid, which has excellent separating properties, high washing efficiency, and easy processability, which uses mass-producible solid phases with substantially uniform separating capacities, and wherein procedures can be automated, and a unit for separation and purification of nucleic acid suitable for implementing this method. The object of the present invention was attained by a method for separation and purification of nucleic acid comprising the steps of adsorbing nucleic acid onto a solid phase and desorbing the nucleic acid from the solid phase, wherein the solid phase comprises a base material in which a graft polymer chain having a hydrophilic group is bonded onto the surface; and a unit for separation and purification of nucleic acid which comprises, in a container having at least two openings, a solid phase of a base material in which a graft polymer chain having a hydrophilic group is bonded onto its surface.

摘要翻译： 本发明的目的是提供一种分离和纯化核酸的方法，其具有优异的分离性能，高洗涤效率和易加工性，其使用具有基本上均匀分离能力的大量生产的固相，并且其中程序可以 自动化，以及用于分离和纯化适用于实施该方法的核酸的单元。 本发明的目的是通过核酸的分离和纯化方法来实现的，其包括将核酸吸附到固相上并从固相中解吸核酸的步骤，其中固相包括基质材料，其中 具有亲水基团的接枝聚合物链被结合到表面上; 以及用于分离和纯化核酸的单元，其在具有至少两个开口的容器中包含其中具有亲水基团的接枝聚合物链键合到其表面上的基材的固相。

公开(公告)号：US06399305B1

公开(公告)日：2002-06-04

申请号：US09588950

申请日：2000-06-07

申请人： Yoshihiko Makino ,  Yoshihiko Abe ,  Makoto Takagi ,  Shigeori Takenaka ,  Kenichi Yamashita ,  Masashi Ogawa

发明人： Yoshihiko Makino ,  Yoshihiko Abe ,  Makoto Takagi ,  Shigeori Takenaka ,  Kenichi Yamashita ,  Masashi Ogawa

IPC分类号： C12Q168

CPC分类号： C12Q1/6825 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2563/113

摘要： A method of analyzing a nucleic acid fragment sample to judge whether the nucleic acid fragment sample is uncomplementary, partly complementary or complementary to a DNA fragment in its specific base sequence is conducted by the steps of: bringing an aqueous solution of the nucleic acid fragment sample into contact with a DNA chip having an electroconductive substrate and the DNA fragment fixed onto the substrate in the presence of an electrochemical thread intercalator; measuring an electric current flowing from or to the electroconductive substrate along the DNA fragment under application of a potential to the substrate; and comparing the electric current measured above with a referential electric current which is prepared employing a DNA chip equivalent to the above DNA chip, the intercalator, and an aqueous solution of a nucleic acid fragment which is complementary to the DNA fragment of the DNA chip.

摘要翻译： 分析核酸片段样品以判断核酸片段样品是否与其特异性碱基序列中的DNA片段不互补，部分互补或互补的方法通过以下步骤进行：使核酸片段样品的水溶液 与具有导电性基体的DNA芯片接触，并且在电化学线性嵌入剂的存在下固定在基材上的DNA片段;测量在施加电位下沿着DNA片段向基片流向或流到导电基底的电流 ; 并使用与上述DNA芯片相当的DNA芯片，嵌入剂和与DNA芯片的DNA片段互补的核酸片段的水溶液制备的参考电流来比较上述电流。

公开(公告)号：US5393493A

公开(公告)日：1995-02-28

申请号：US993459

申请日：1992-12-15

申请人： Yoshihiko Makino ,  Kaoru Terashima ,  Toru Kitani ,  Naofumi Hora

发明人： Yoshihiko Makino ,  Kaoru Terashima ,  Toru Kitani ,  Naofumi Hora

IPC分类号： G01N31/22 ,  B01L3/00 ,  B01L99/00 ,  C12Q1/48 ,  C12Q1/60 ,  G01N21/78 ,  G01N33/49 ,  G01N33/52 ,  G01N33/92

CPC分类号： G01N33/525 ,  C12Q1/48 ,  C12Q1/60

摘要： An integral multilayer analytical element in which a first fibrous porous layer, a nonfibrous porous layer, and a second fibrous porous layer superposed in this order on a water-impermeable light-transmissive support the first fibrous porous-layer having a larger pore size than the nonfibrous porous layer. The above three porous layers are integrally laminated to each other substantially closely by an adhesive discontinuously disposed so as to form microspaces continuing through from one layer to the next so as not to interfere with the approximately uniform permeation of liquid. A reagent composition which produces an optically detectable change in the presence of an analyte is incorporated in at least one of said three porous layers. When a nonporous reagent layer is incorporated on the support in the above analytical element, the reagent composition is incorporated in the nonporous reagent layer. By using the analytical element of the invention, the analytical values of various analytes of blood samples can be obtained independently of hematocrit values from whole blood samples and blood plasma samples in the range of the hematocrit values of 0% to 55%.

摘要翻译： 一种整体的多层分析元件，其中第一纤维多孔层，非纤维多孔层和第二纤维多孔层依次叠加在不透水的透光性上，支撑第一纤维多孔层，其孔径大于 非纤维多孔层。 上述三个多孔层通过不连续地布置的粘合剂基本上紧密地彼此层压，以便形成从一层到下一层的连续通过的微孔，以便不干扰液体的大致均匀渗透。 在分析物存在下产生可光学检测的变化的试剂组合物结合在所述三个多孔层中的至少一个中。 当在上述分析元件中的载体上并入无孔试剂层时，将试剂组合物并入无孔试剂层。 通过使用本发明的分析元件，可以在血细胞比容值为0％至55％的范围内，独立于来自全血样品和血浆样品的血细胞比容值而独立地获得血液样品的各种分析物的分析值。