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    Rennin from recombinant microbial cells for preparation of cheese
    4.
    发明授权
    Rennin from recombinant microbial cells for preparation of cheese 失效
    来自重组微生物细胞的Rennin用于制备奶酪

    公开(公告)号：US4935354A

    公开(公告)日：1990-06-19

    申请号：US927778

    申请日：1986-11-05

    申请人： Kirk J. Hayenga ,  Virgil B. Lawlis

    发明人： Kirk J. Hayenga ,  Virgil B. Lawlis

    IPC分类号： A23C19/032 ,  C07K1/113 ,  C12N1/06 ,  C12N9/52 ,  C12N9/64 ,  C12N15/70

    CPC分类号： C12N9/6481 ,  A23C19/0326 ,  C07K1/1133 ,  C12N1/06 ,  C12N15/70 ,  C12N9/52

    摘要： Rennin for making cheese is obtained from insoluble refractile bodies of a recombinant microbial host cell. The rennin is obtained by rupturing the recombinant host cell, isolating and solubilizing the insoluble refractile bodies, and recovering active rennin. Recombinant techniques involve preparing cDNA corresponding to the coding sequence for calf rennin, introducing into an expression vector and expressing in a host cell. As much as 200 mg rennin per liter of culture may be recovered. Prorennin or preprorennin may be produced and rennin derived therefrom.

    摘要翻译： 用于制作奶酪的Rennin由重组微生物宿主细胞的不溶性折射体获得。 通过破坏重组宿主细胞，分离和溶解不溶性折射体，并回收活性肾素获得肾素。 重组技术涉及制备对应于小牛肾素编码序列的cDNA，引入表达载体并在宿主细胞中表达。 每升培养物可以回收多达200毫克的肾素。 可以产生丙氨宁或前肾上腺素，并从中衍生肾素。

    Heterologous polypeptides expressed in aspergillus
    10.
    发明授权
    Heterologous polypeptides expressed in aspergillus 失效
    在曲霉中表达的异源多肽

    公开(公告)号：US5364770A

    公开(公告)日：1994-11-15

    申请号：US413010

    申请日：1989-09-25

    申请人： Randy M. Berka ,  Daniel Cullen ,  Gregory L. Gray ,  Kirk J. Hayenga ,  Virgil B. Lawlis

    发明人： Randy M. Berka ,  Daniel Cullen ,  Gregory L. Gray ,  Kirk J. Hayenga ,  Virgil B. Lawlis

    IPC分类号： C12N1/15 ,  C12N1/19 ,  C12N9/34 ,  C12N9/58 ,  C12N9/64 ,  C12N15/62 ,  C12N15/80 ,  C12N15/09 ,  C12N15/56

    CPC分类号： C12N9/2428 ,  C12N15/62 ,  C12N15/80 ,  C12N9/58 ,  C12N9/6481 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/50 ,  C07K2319/61

    摘要： Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3' terminus of the DNA sequence encoding the heterologous polypeptides. Each of the above described vectors are used in novel processes to transform a filamentous fungus wherein the DNA sequences encoding the signal sequence and heterologous polypeptide are expressed. The thus synthesized polypeptide is thereafter secreted from the filamentous fungus.

    摘要翻译： 公开了用于从丝状真菌表达和分泌异源多肽的新型载体。 这些载体用于表达和分泌这些异源多肽的新方法中。 用于转化丝状真菌以表达和分泌异源多肽的载体包括编码异源多肽的DNA序列和编码在给定丝状真菌中在分泌系统中有功能的信号序列的DNA序列，并且可操作地连接到 编码异源多肽的序列。 这样的信号序列可以是通常与异源多肽相关的信号序列，或者可以来自其他来源。 载体还可以含有编码启动子序列的DNA序列，其由丝状真菌功能识别，并且可操作地连接于编码信号序列的DNA序列。 优选地，功能性多腺苷酸化序列可操作地连接到编码异源多肽的DNA序列的3'末端。 上述载体中的每一种用于转化丝状真菌的新方法，其中表达编码信号序列和异源多肽的DNA序列。 由此合成的多肽此后由丝状真菌分泌。