公开(公告)号：US20180237815A1

公开(公告)日：2018-08-23

申请号：US15750792

申请日：2016-06-17

Applicant: KIKKOMAN CORPORATION

Inventor： Keiichi ICHIKAWA ,  Yasutomo SHINOHARA ,  Seiichi HARA ,  Keiko KUROSAWA ,  Yumiko YAMASHITA ,  Michiaki YAMASHITA

IPC: C12P17/10 ,  C12R1/685 ,  C12N1/14 ,  C12N15/09 ,  C12R1/665 ,  C12R1/69

CPC classification number: C12P17/10 ,  C12N1/14 ,  C12N15/09 ,  C12R1/66 ,  C12R1/665 ,  C12R1/685 ,  C12R1/69

Abstract: The purpose of the present invention is to provide a method for producing selenoneine that allows production of selenoneine at higher yields as compared with a conventional technology, and, therefore, enables selenoneine production on an industrial scale. This purpose can be achieved by a method for producing selenoneine, comprising the step of applying histidine and a selenium compound to a transformant that has a gene encoding an enzyme of (1) below introduced therein and that can overexpress the introduced gene, to obtain selenoneine. (1) An enzyme that catalyzes a reaction in which hercynylselenocysteine is produced from histidine and selenocysteine in the presence of S-adenosylmethionine and iron (II).

公开(公告)号：US5840570A

公开(公告)日：1998-11-24

申请号：US345018

申请日：1994-11-23

Applicant: Randy M. Berka ,  Kirk J. Hayenga ,  Virgil B. Lawlis ,  Michael Ward

Inventor： Randy M. Berka ,  Kirk J. Hayenga ,  Virgil B. Lawlis ,  Michael Ward

IPC: A23C19/04 ,  C12N1/15 ,  C12N9/62 ,  C12N9/64 ,  C12N15/80 ,  C12N15/09 ,  C12N15/12

CPC classification number: C12N9/6481 ,  A23C19/04 ,  C12N15/80 ,  C12N9/62 ,  C12R1/665

Abstract: This invention relates to novel mutant filamentous fungi which are deficient in the gene for the corresponding aspartic proteinase. These organisms are useful production hosts in the production of heterologous polypeptides such as chymosin.

公开(公告)号：US20170321235A1

公开(公告)日：2017-11-09

申请号：US15533215

申请日：2015-12-25

Applicant: KIKKOMAN CORPORATION

Inventor： Seiichi HARA ,  Keiko KUROSAWA ,  Keiichi ICHIKAWA

IPC: C12P13/04 ,  C12N9/10

CPC classification number: C12P13/04 ,  C12N9/10 ,  C12N9/1007 ,  C12N9/13 ,  C12N15/09 ,  C12R1/66 ,  C12R1/665 ,  C12R1/685 ,  C12R1/69 ,  C12Y201/01 ,  C12Y208/01007

Abstract: The purpose of the present invention is to provide an organism having an ergothioneine productivity that is capable of easily producing ergothioneine within a short period of time at a high yield, as compared with a conventional technology, and, therefore, enables ergothioneine production on an industrial scale. This purpose can be achieved by a transformed fungus into which a gene encoding enzyme (1) or genes encoding enzymes (1) and (2) have been inserted and in which the inserted gene(s) are overexpressed. (1) an enzyme catalyzing a reaction of synthesizing hercynyl cysteine sulfoxide from histidine and cysteine in the presence of S-adenosyl methionine, iron (II) and oxygen. (2) An enzyme catalyzing a reaction of synthesizing ergothioneine from hercynyl cysteine sulfoxide using pyridoxal 5′-phosphate as a coenzyme.

公开(公告)号：US06509171B1

公开(公告)日：2003-01-21

申请号：US09048473

申请日：1998-03-26

Applicant: Randy M. Berka ,  Kirk J. Hayenga ,  Virgil B. Lawlis ,  Michael Ward

Inventor： Randy M. Berka ,  Kirk J. Hayenga ,  Virgil B. Lawlis ,  Michael Ward

IPC: C12P2102

CPC classification number: C12N9/6481 ,  A23C19/04 ,  C12N9/62 ,  C12N15/80 ,  C12R1/665

Abstract: This invention relates to novel mutant filamentous fungi which are deficient in the gene for the corresponding aspartic proteinase. These organisms are useful production hosts in the production of heterologous polypeptides such as chymosin.

Abstract translation: 本发明涉及缺乏相应天冬氨酸蛋白酶基因的新型突变丝状真菌。 这些生物体是生产异源多肽如凝乳酶的有用生产宿主。